51 Works

Healthy Outcomes of Patients with COVID-19 Two Years after the Infection: A Prospective Cohort Study

Dapeng Li, Xuejiao Liao, Zhi Liu, Zhenghua Ma, Jingke Dong, Guoqin Zheng, Mei Zi, Fang Wang, Qing He, Guobao Li, Zheng Zhang & Lei Liu
The long-term effect of coronavirus disease 2019 (COVID-19) has been rarely known. This study aimed to investigate healthy outcomes of COVID-19 survivors up to 2 years after the infection. A total of 155 COVID-19 patients, who were discharged from Shenzhen Third People's Hospital from February 2020 to April 2020, were enrolled and followed up until March 4, 2022. COVID-19 survivors received questionnaires of long COVID symptoms and psychological symptoms, pulmonary function tests, chest computed tomography...

Identification of mite-specific eosinophils in the colon of patients with ulcerative colitis

Shu-Wang Peng, Jiang-Ming Sheng, Bai-Sui Feng, Ke-Ping Peng, Gui-Xiang Tian, Cheng-Bai Liang, Ming-Hui Liu, Hai-Qing Xie, Qing Shu, Yan Li & Ping-Chang Yang
The pathogenesis of ulcerative colitis (UC) is unclear. House dust mite (HDM) is associated with immune inflammation in the body. This study is designed to identify the association between HDM and UC clinical symptoms. UC patients (n = 86) and non-UC control (NC) subjects (n = 64) were recruited. Colon lavage fluids (CLF) were collected from HDM skin prick test positive patients during colonoscopy, and analyzed by immunological approaches. HDM was detected in fecal samples,...

Semaphorin 3A Inhibits Endoplasmic Reticulum Stress Induced by High Glucose in Müller Cells

Mengyang Li, Enzhong Jin, Li Zhu, Chi Ren, Zhiqiao Liang, Mingwei Zhao & Jinfeng Qu
This study aimed to explore the effect of the Semaphorin3A (Sema3A)/Neuropilin-1 (Nrp-1) pathway on Müller cell activities and endoplasmic reticulum (ER) stress induced by high glucose (HG) in vitro. The primary Müller cells of C57BL/6J mice were isolated and cultured in normal or high glucose medium. The expression of endogenous Sema3A and its coreceptor Nrp-1 was measured by Western blot. Müller cells were incubated with exogenous recombinant Sema3A protein or transfected with lentiviral vectors expressing...

Cloning, homology modeling, heterologous expression and bioinformatic analysis of Ure2pA glutathione S-transferase gene from white rot fungus Trametes gibbosa

Jian Zhang, Yujie Chi, Shuxuan Li, Xinzhi Gu & Yi Ye
Glutathione S-transferases (GSTs) are a class of enzymes with detoxification function. GSTs can be used for the degradation of toxic aromatic compounds, such as dyes. In order to promote their application and cost-effective dye degradation and to understand their structure and function more deeply, we cloned and expressed a gst2 gene from Trametes gibbosa—a white rot fungus. T.gibbosa gst2 has a 672-bp open reading frame (ORF) and encodes 223 amino acids. The recombinant Tg-gst2 in...

Identification of key candidate genes for wing length-related traits by whole-genome resequencing in 772 geese

Jing Ouyang, Yongfei Wu, Yaxi Li, Junjie Miao, Sumei Zheng, Hongbo Tang, Cong Wang, Yanpeng Xiong, Yuren Gao, Luping Wang, Xueming Yan & Hao Chen
1. A total of 772, 420-day-old Xingguo gray geese (XGG) were sequenced using a low-depth (~1×) whole-genome resequencing strategy to reveal the genetic mechanism of wing length-related traits by genome-wide association analysis (GWAS). 2. The results showed that 119 SNPs had genome-wide significance for wing length in five regions of chromosome 4, of which the most significant locus (P=7.95E-11) was located upstream of RBM47 and explained 7.3% of phenotypic variation. 3. A total of 219...

Using spectrum allocations to address indigenous policy obligations: the case of New Zealand

Bronwyn Howell & Xin Tang
Radio spectrum is fundamental to the operation of wireless communications services. In order to allocate radio spectrum for different services, spectrum is regulated via national laws, coordinated by an international body, the International Telecommunications Union (ITU). For the most part, national (state) governments undertake the legal definition and allocation of spectrum. The most common method of allocation is auction, where competing parties bid for the rights to use specific bundles of spectrum, ensuring that the...

A carbon felt cathode modified by acidic oxidized carbon nanotubes for the high H2O2 generation and its application in electro-Fenton

Ying Gao, Fangshu Xie, Huiling Bai, Li Zeng, Jingbin Zhang, Meiyu Liu & Weihuang Zhu
Herein, a carbon felt (CF) cathode modified by the acidic oxidized carbon nanotubes (OCNTs) exhibited a high yield of the H2O2 generation in electro-Fenton. Rotating disk electrode (RDE) measurements showed that the selective generation of H2O2 occurred on the CF cathode coated by OCNTs (OCNTs/CF), which was attributed to the high amount of the oxygen-containing functional groups in OCNTs. Moreover, the pollutant degradation efficiency could almost reach 100% within 60 min in electro-Fenton with OCNTs/CF...

Logistics choices in a platform supply chain: A co-opetitive perspective

Pei Wang, Shaofu Du, Li Hu & Wenzhi Tang
With the popularity and development of the platform economy, numerous enterprises are choosing to cooperate with e-commerce platforms to expand online market, and the cooperation modes have exhibited innovation and diversification. This paper focuses on a new platform-based co-opetitive supply chain whereby a flagship store operated by the manufacturer (MFS) and a self-run store operated by the platform (PSS) coexist in the platform market. The manufacturer acts as the platform’s upstream contract manufacturer and downstream...

Total RNA sequencing of Phlebotomus chinensis, a neglected vector in China, simultaneously revealed viral, bacterial, and eukaryotic microbes that are potentially pathogenic to humans

Jing Wang, Qin-yu Gou, Geng-yan Luo, Xin Hou, Guodong Liang & Mang Shi
Phlebotomus chinensis sandfly is a neglected insect vector in China which is well-known for carrying Leishmania. Recent studies have expanded its pathogen repertoire with two novel arthropod-borne phleboviruses capable of infecting human and animals. Despite these discoveries, our knowledge on the general pathogen diversity and overall microbiome composition of this vector species are still very limited. Here we carried out a meta-transcriptomics analysis which simultaneously revealed the actively replicating/transcribing RNA viruses, DNA viruses, bacteria and...

Efficacy of heterologous boosting against SARS-CoV-2 using a recombinant interferon-armed fusion protein vaccine (V-01): a randomized, double-blind and placebo-controlled phase III trial

Xuan-Yi Wang, Syed Faisal Mahmood, Fang Jin, Wee Kooi Cheah, Muhammad Ahmad, Mian Amjad Sohail, Waheed Ahmad, Vijaya K Suppan, Muneeba Ahsan Sayeed, Shobha Luxmi, Aik-Howe Teo, Li Yuan Lee, Yang-Yang Qi, Rong-Juan Pei, Wei Deng, Zhong-Hui Xu, Jia-Ming Yang, Yan Zhang, Wu-Xiang Guan & Xiong Yu
Waning of neutralizing titers along with decline of protection efficacy after the second dose of COVID-19 vaccines was observed, including China-made inactivated vaccines. Efficacy of a heterologous boosting using one dose of a recombinant SARS-CoV-2 fusion protein vaccine (V-01) in inactivated vaccine-primed population was studied, aimed to restore the immunity. A randomized, double-blind and placebo-controlled phase Ⅲ trial was conducted in healthy people aged 18 years or older in Pakistan and Malaysia. Each eligible participant...

RT-LAMP assay for rapid detection of the R203M mutation in SARS-CoV-2 Delta variant

Jianing Yang, Xuejiao Hu, Wenzhuo Wang, Yujing Yang, Xinqiang Zhang, Wei Fang, Lei Zhang, Shan Li & Bing Gu
The highly infectious Delta variant strain of SARS-CoV-2 remains globally dominant and undermines COVID-19 vaccines. Rapid detection of the Delta variant is crucial for the identification and quarantine of infected individuals. In this study, our aim was to design and validate a genotyping RT-LAMP method to detect Delta variants specifically. R203M in the N gene of SARS-CoV-2 was chosen as the Delta variant-specific mutation for genotyping. To target the R203M-harboring region and the conserved sequence...

MERS-CoV nsp1 regulates autophagic flux via mTOR signaling and dysfunctional lysosomes

Yujie Feng, Zhaoyi Pan, Zhihui Wang, Zhengyang Lei, Songge Yang, Huajun Zhao, Xueyao Wang, Yating Yu, Qiuju Han & Jian Zhang
Autophagy, a cellular surveillance mechanism, plays an important role in combating invading pathogens. However, viruses have evolved various strategies to disrupt autophagy and even hijack it for replication and release. Here, we demonstrated that Middle East respiratory syndrome coronavirus (MERS-CoV) non-structural protein 1(nsp1) induces autophagy but inhibits autophagic activity. MERS-CoV nsp1 expression increased ROS and reduced ATP levels in cells, which activated AMPK and inhibited the mTOR signaling pathway, resulting in autophagy induction. Meanwhile, as...

Myostain is involved in ginsenoside Rb1-mediated anti-obesity

Hong-Shi Li, Jiang-Ying Kuang, Gui-Jun Liu, Wei-Jie Wu, Xian-Lun Yin, Hao-Dong Li, Lei Wang, Tao Qin, Wen-Cheng Zhang & Yuan-Yuan Sun
Obesity, one of the major public health problems worldwide, has attracted increasing attention. Ginsenoside Rb1 is the most abundant active component of Panax ginseng C.A.Mey (Araliaceae) and is reported to have beneficial effects on obesity and diabetes. However, the mechanisms by which Rb1 regulates obesity remain to be explored. This paper intends to further explore the mechanism of Rb1 in regulating obesity. The C57BL/6 obese mice were divided into two groups: the control (CTR) and...

Impact of vaccination on kinetics of neutralizing antibodies against SARS-CoV-2 by Serum live neutralization test based on a prospective cohort

Liguo Zhu, Naiying Mao, Changhua Yi, Aidibai Simayi, Jialu Feng, Yi Feng, Min He, Songning Ding, Yin Wang, Yan Wang, Mingwei Wei, Jie Hong, Chuchu Li, Hua Tian, Lu Zhou, Jiefu Peng, Shihan Zhang, Ci Song, Hui Jin, Fengcai Zhu, Wenbo Xu, Jun Zhao & Changjun Bao
How much the vaccine contributes to the induction and development of neutralizing antibodies (NAbs) of breakthrough cases relative to those unvaccinated-infected cases is not fully understood. We conducted a prospective cohort study and collected serum samples from 576 individuals who were diagnosed with SARS-CoV-2 Delta strain infection, including 245 breakthrough cases and 331 unvaccinated-infected cases. NAbs were analyzed by live virus microneutralization test and transformation of NAb titer. NAbs titers against SARS-CoV-2 ancestral and Delta...

Integrated analysis of long non-coding RNAs and mRNA expression profiles identified potential interactions regulating melanogenesis in chicken skin

Shigang Yu, Gang Wang, Juan Liao, Xuemei Shen & Jia Chen
1. Long non-coding RNAs (lncRNAs) play important roles in various physiological functions. However, the mechanisms underlying the regulation of lncRNAs in melanogenesis remain unclear. To determine the molecular mechanisms involved in the melanogenesis of skin melanogenesis, the present study depicted the expression profiles of lncRNAs and messenger RNAs (mRNAs) in black- (B group) and white- (W group) skinned chickens using RNA sequencing. 2. In total, 373 differentially expressed lncRNAs (DELs; 203 up-regulated and 170 down-regulated)...

Effect of wounding and wound age on infection of canola cotyledons by Leptosphaeria maculans, interacting with leaf wetness

Shuanglong Huang, Chun Zhai, Zhongwei Zou, Fei Liu, Paula Parks, Linda Mcgregor, W. G. Dilantha Fernando & Gary Peng
Blackleg, caused by the fungus Leptosphaeria maculans, is an important disease of canola. Both ascospores and pycnidiospores of the fungus can infect intact leaves under conditions of extended leaf wetness, with infection then progressing further into the stem. In western Canada, spring is typically cool and dry and additional factors may be involved for successful infection. This study was designed to assess the effect of wounding and wound age on cotyledon infection, to evaluate the...

Booster vaccination is required to elicit and maintain COVID-19 vaccine-induced immunity in SIV-infected macaques

Pingchao Li, Qian Wang, Yizi He, Chenchen Yang, Zhengyuan Zhang, Zijian Liu, Bo Liu, Li Yin, Yilan Cui, Peiyu Hu, Yichu Liu, Pingqian Zheng, Wei Wang, Linbing Qu, Caijun Sun, Suhua Guan, Liqiang Feng & Ling Chen
Prolonged infection and possible evolution of SARS-CoV-2 in patients living with uncontrolled HIV-1 infection highlight the importance of an effective vaccination regimen, yet the immunogenicity of COVID-19 vaccines and predictive immune biomarkers have not been well investigated. Herein, we report that the magnitude and persistence of antibody and cell-mediated immunity (CMI) elicited by an Ad5-vectored COVID-19 vaccine are impaired in SIV-infected macaques with high viral loads (> 105 genome copies per ml plasma, SIVhi) but...

Logistics choices in a platform supply chain: A co-opetitive perspective

Pei Wang, Shaofu Du, Li Hu & Wenzhi Tang
With the popularity and development of the platform economy, numerous enterprises are choosing to cooperate with e-commerce platforms to expand online market, and the cooperation modes have exhibited innovation and diversification. This paper focuses on a new platform-based co-opetitive supply chain whereby a flagship store operated by the manufacturer (MFS) and a self-run store operated by the platform (PSS) coexist in the platform market. The manufacturer acts as the platform’s upstream contract manufacturer and downstream...

Decreased echinocandin susceptibility in Candida parapsilosis causing candidemia and emergence of a pan-echinocandin resistant case in China

Yating Ning, Meng Xiao, David S. Perlin, Yanan Zhao, Minya Lu, Yi Li, Zhengyu Luo, Rongchen Dai, Shengjie Li, Jiajun Xu, Lingli Liu, Hong He, Yun Liu, Fushun Li, Yuguang Guo, Zhongju Chen, Yingchun Xu, Tianshu Sun & Li Zhang
Candida parapsilosis is becoming a predominant non-albicans cause of invasive candidiasis (IC). Echinocandins are the preferred choice for IC treatment and prophylaxis. Resistance to echinocandins in C. parapsilosis has emerged in several countries, but little is known about the susceptibility profile in China or about mechanisms of resistance. Here, we investigated the echinocandin susceptibilities of 2523 C. parapsilosis isolates collected from China and further explored the resistance mechanism among echinocandin-resistant isolates. Anidulafungin exhibited the highest...

Bimetallic Mn-Ce loaded on different zeolite carriers applied in the toluene abatement in air by non–thermal plasma DDBD Reactor

Su Liu, Jiabin Zhou, Dan Liu, Xianjie Liu & Wenbo Liu
A sequence of zeolite carriers (Carrier = ZSM-5, Small crystal ZSM-5, MCM-41, SBA-15) were used to support active metals Mn-Ce, which have presented an enormous potential for plasma oxidation of toluene in air. The prepared samples were detected by means of N2 adsorption-desorption, SEM, XPS, H2-TPR, etc. Through the activity evaluation in the Non-thermal Plasma Reactor, we found that the catalysts with different carriers showed distinct degradation activities. The performance of mesoporous supported catalysts was...

Different cellular mechanisms from low- and high-dose zinc oxide nanoparticles-induced heart tube malformation during embryogenesis

Mengwei Wang, Ping Zhang, Zeyu Li, Yu Yan, Xin Cheng, Guang Wang & Xuesong Yang
With the wide application of nanometer materials in daily life, people pay more attention to the potential toxicity of nanoparticles to human fetal development once the nanoparticles are absorbed into the human body during pregnancy. However, there was no directly solid evidence for ZnO NPs-caused congenital heart defects. Hence, we investigated the effect of ZnO NPs exposure on early cardiogenesis using the chicken/mouse embryo models. First, we showed ZnO NPs reduced H9c2 cell viability in...

Spectral correlation between wheat genotype replications over the visible and near-infrared spectrum

Xuebin Yang, Xu Wang, Byron Evers, Yaokui Cui & Jesse Poland
The growth stage best suitable for wheat yield phenotyping has been a hot topic. This study provides a fresh insight into it, from the perspective of spectral correlation between wheat genotype replications. A number of 340 wheat genotypes and their replication were distributed in separate parts (west and east) of an experiment field (2019–2020) in Ashland, Kansas, USA. Unmanned aerial vehicle based hyperspectral images (400–100 nm) of the experiment field were taken over the late...

A Time-Varying Network for Cryptocurrencies

Li Guo, Wolfgang Karl Härdle & Yubo Tao
Cryptocurrencies return cross-predictability and technological similarity yield information on risk propagation and market segmentation. To investigate these effects, we build a time-varying network for cryptocurrencies, based on the evolution of return cross-predictability and technological similarities. We develop a dynamic covariate-assisted spectral clustering method to consistently estimate the latent community structure of cryptocurrencies network that accounts for both sets of information. We demonstrate that investors can achieve better risk diversification by investing in cryptocurrencies from different...

METTL21C mediates lysine trimethylation of IGF2BP1 to regulate chicken myoblast proliferation

Shanshan Wang, Jiarong Zhao, Ling Wang, Tao Zhang, Wenxian Zeng & Hongzhao Lu
1. Methyltransferase-like 21C (METTL21C) and insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1) play important roles in the proliferation of chicken myoblasts. However, it remains unclear whether there is protein-protein interaction between METTL21C and IGF2BP1 to regulate proliferation of chicken myoblasts. 2. In this study, the Igf2bp1 gene was amplified from cDNA of liver tissue of Lueyang black-bone chicken to construct the overexpression vector HA-Igf2bp1. The HA-Igf2bp1 and Flag-Mettl21c vectors were individually transfected and co-transfected...

FBXO34 promotes latent HIV-1 activation by post-transcriptional modulation

Xinyi Yang, Xiaying Zhao, Yuqi Zhu, Jingna Xun, Qin Wen, Hanyu Pan, Jinlong Yang, Jing Wang, Zhimin Liang, Xiaoting Shen, Yue Liang, Qinru Lin, Huitong Liang, Min Li, Jun Chen, Shibo Jiang, Jianqing Xu, Hongzhou Lu & Huanzhang Zhu
Acquired immunodeficiency syndrome (AIDS) cannot be completely cured, mainly due to the existence of a latent HIV-1 reservoir. However, our current understanding of the molecular mechanisms underlying the establishment and maintenance of HIV-1 latent reservoir is not comprehensive. Here, using a genome-wide CRISPR-Cas9 activation library screening, we identified E3 ubiquitin ligase F-box protein 34 (FBXO34) and the substrate of FBXO34, heterogeneous nuclear ribonucleoprotein U (hnRNP U) was identified by affinity purification mass spectrometry, as new...

Registration Year

  • 2022

Resource Types

  • Other


  • Sichuan University
  • Shanghai Jiao Tong University
  • Chinese Academy of Medical Sciences & Peking Union Medical College
  • Zhejiang University
  • Fudan University
  • Sun Yat-sen University
  • Huazhong University of Science and Technology
  • Shandong University
  • University of Chinese Academy of Sciences
  • Capital Medical University