2 Works

MosTI single-copy transgene insertion in C. elegans using constitutive Cas9 expression

Sonia El Mouridi & Christian Frøkjær-Jensen
Transgene expression in C. elegans is an important tool for understanding regulatory elements (e.g., promoters and enhancers), for controlling cells using optogenetic effector proteins, and for editing the (epi)genome. Insertion of single-copy transgenes into well-defined safe-harbor locations can be used when consistent expression is required (e.g., to compare expression from different transgenes) or when expression is required in germ cells. Here, we describe a detailed protocol for inserting single-copy transgenes using Modular Safe-harbor Transgene Insertion...

Protocol for the continuous generation of single-copy MosTI insertions in C. elegans from a multiplex mix.

Sonia El Mouridi & Christian Frøkjær-Jensen
Precise expression of transgenes in C. elegans can be used to understand gene regulation, control cells, or as a starting point for genetic screens. Inserting singlecopy transgenes into well-defined safe-harbor locations is useful when consistent expression levels are required (e.g., to compare expression from different transgenes) or when expression is desired in germ cells. Here, we describe a detailed protocol for the simultaneous injection of several different plasmids ("multiplex mix"). Heat-shock inducible Cas9 expression allows...

Registration Year

  • 2022
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Resource Types

  • Other
    2

Affiliations

  • King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia
    2