148 Works

Daidzein stimulates fatty acid-induced fat deposition in C2C12 myoblast cells via the G protein-coupled receptor 30 pathway

Chengjian Zhou, Ping Li, Meihong Han & Xuejun Gao
Fat deposition in skeletal muscle is an important aspect of improving meat quality. Isoflavones can promote animal anabolism, but whether and how they regulate muscle fat deposition remain largely unclear. In this study, we explored the role and corresponding molecular mechanism of one of the major isoflavones, daidzein, in fat deposition in C2C12 myoblast cells. In the absence of fatty acids (FAs), daidzein did not promote triglyceride synthesis and lipid droplet formation in cells but...

Nanoparticle-mediated delivery of Tanshinone IIA reduces adverse cardiac remodeling following myocardial infarctions in a mice model: role of NF-κB pathway

Shuai Mao, Lei Wang, Peipei Chen, Yong Lan, Rui Guo & Minzhou Zhang
Our previous works have shown that tanshinone IIA inhibited maladaptive extracellular matrix remodeling in cardiac fibroblasts implicating its potential role in treating of pathologic cardiac remodeling. However, the intrinsically poor solubility and bioavailability of tanshinone IIA hindered its clinical application. Here we develop monomethoxy-poly (ethylene glycol)-poly (lactic acid)-D-α-Tocopheryl polyethylene glycol 1000 succinate (mPEG-PLA-TPGS) nanoparticle incorporating tanshinone IIA (tanshinone IIA-NPs) and study its efficacy in post-infarction left ventricular (LV) remodeling. Male C57BL/6 mice underwent left coronary...

Angiopoietin-like protein 8, molecular cloning and regulating lipid accumulation in goat intramuscular preadipocytes

Wei Huang, Yaqiu Lin, Hua Xiang, Jiangjiang Zhu & Yong Wang
This study aimed to clone the full-length open reading frame (ORF) of goat ANGPTL8 gene sequence, reveal its molecular and expression characteristics, and explore its effect on the differentiation of goat intramuscular preadipocytes. The full-length ORF sequence of goat ANGPTL8 gene was cloned by RT-PCR technology, and bioinformatics analysis was performed by related biological software. RT-qPCR was used to detect the expression of ANGPTL8 mRNA in goat tissues. Further use of RNA interference to study...

Perchlorate bioreduction in UASB reactor: S2--autotrophic granular sludge formation and sulfate generation control

Rui Zhao, Huaqiang Tao, Yuanyuan Song, Jianbo Guo, Zhi Chen, Caicai Lu, Yi Han, Haibo Li & Yanan Hou
Perchlorate (ClO4-) industrial wastewater requires efficient removal to prevent adverse environmental impacts, however, high concentration and low biodegradability give rise to poor ClO4- bioreduction performance. S2--autotrophic granular sludge (S2--AuGS) was firstly cultivated for high concentration perchlorate (ClO4-) removal in the Upflow Anaerobic Sludge Blanket (UASB) reactor (ClO4-: 150 mg·L-1). Simultaneously, the S2- was utilized to control the SO42- generation as electron donor, the effluent SO42- concentration (190 mg·L-1) was satisfied with drinking water standard (250...

Batch-to-batch consistency trial of an adenovirus type-5 vector-based COVID-19 vaccine in adults aged 18 years and above

Zhuo-Pei Li, Yun-Feng Shi, Li-Hua Hou, Peng-Fei Jin, Shi-Hua Ma, Hong-Xing Pan, Jin-Long Zhang, Yong-Mei Shan, Hai-Tao Huang, Shi-Po Wu, Pan Du, Xue Wang, Li-Li Wang, Rui-Jie Wang, Ying Wang, Xue-Wen Wang, Feng-Cai Zhu & Jing-Xin Li
The demonstration of batch-to-batch consistency is indispensable for quality control of vaccines. We did a randomized, double-blind, parallel-controlled trial to evaluate the immunogenicity consistency of one shot of Ad5-nCoV in healthy adults who had not received any COVID-19 vaccine before. All eligible participants were randomly assigned equally to receive one of three consecutive batches of Ad5-nCoV (5×1010 viral particles/vial, 0.5 mL). The primary endpoint was geometric mean titers (GMTs) of serum SARS-CoV-2 receptor binding domain...

A Population Pharmacokinetic-Pharmacodynamic Model of Navtemadlin, its Major Active Metabolite (M1) and Serum Macrophage Inhibitory Cykokine-1 (MIC-1)

Lu Zhang, Bill Poland, Michelle Green, Shekman Wong & J. Greg Slatter
Navtemadlin is a potent, selective, orally available inhibitor of murine double minute 2 that restores p53 activity to induce apoptosis in TP53 wild-type malignancies. Using richly sampled pharmacokinetic (PK) and pharmacodynamic (PD) data from healthy volunteers, a population PK/PD model was developed. A population PK (PPK) model described the PK characteristics of navtemadlin and its major metabolite acyl glucuronide (M1) and quantified enterohepatic recirculation (EHR). Post hoc individual PK parameters from this model were coupled...

Surface Thermal and Moisture Behavior of Wool Fabrics Treated with Hydrogen Peroxide

Ling Lin, Chunxia Wang, Haiyan Mao, Huanling Wu & Zhipeng Ma
Understanding the surface comfort of wool fabrics with modified surface is essential for the application of wool. This work aims to reveal the surface wettability and the surface thermal and moisture behavior of wool fabrics modified by hydrogen peroxide with different concentrations. The water absorbing time and wetting area of the treated fabrics were recorded, and the wick heights were measured to understand the surface wettability of the modified wool fabrics. The thermal resistance and...

Omicron infections profile and vaccination status among 1881 liver transplant recipients: a multi-center retrospective cohort

Ensi Ma, Jingwen Ai, Yi Zhang, Jianming Zheng, Xiaogang Gao, Junming Xu, Hao Yin, Zhiren Fu, Hao Xing, Li Li, Liying Sun, Heyu Huang, Quanbao Zhang, Linlin Xu, Yanting Jin, Rui Chen, Guoyue Lv, Zhijun Zhu, Wenhong Zhang & Zhengxin Wang
A wave of Omicron infections rapidly emerged in China in 2022, but large-scale data concerning the safety profile of vaccines and Coronavirus disease 2019 (COVID-19) infection features in liver transplant (LT) recipients have not been collected. Therefore, the aim of this study was to assess the protectiveness and safety profile of the inactivated vaccines in LT patients against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron variant infections. A multi-center retrospective study was conducted in...

Booster vaccination is required to elicit and maintain COVID-19 vaccine-induced immunity in SIV-infected macaques

Pingchao Li, Qian Wang, Yizi He, Chenchen Yang, Zhengyuan Zhang, Zijian Liu, Bo Liu, Li Yin, Yilan Cui, Peiyu Hu, Yichu Liu, Pingqian Zheng, Wei Wang, Linbing Qu, Caijun Sun, Suhua Guan, Liqiang Feng & Ling Chen
Prolonged infection and possible evolution of SARS-CoV-2 in patients living with uncontrolled HIV-1 infection highlight the importance of an effective vaccination regimen, yet the immunogenicity of COVID-19 vaccines and predictive immune biomarkers have not been well investigated. Herein, we report that the magnitude and persistence of antibody and cell-mediated immunity (CMI) elicited by an Ad5-vectored COVID-19 vaccine are impaired in SIV-infected macaques with high viral loads (> 105 genome copies per ml plasma, SIVhi) but...

Charge-reversal nanomedicine based on black phosphorus for the development of A Novel photothermal therapy of oral cancer

Zimu Li, Qingyun Liu, Yi Zhang, Yao Yang, Xiaomin Zhou, Wei Peng, Zhigang Liang, Xiaowei Zeng, Qiuxu Wang & Nansha Gao
Driven by the lifestyle habits of modern people, such as excessive smoking, drinking, and chewing betel nut and other cancer-causing foods, the incidence of oral cancer has increased sharply and has a trend of becoming younger. Given the current mainstream treatment means of surgical resection will cause serious damage to many oral organs, so that patients lose the ability to chew, speak, and so on, it is urgent to develop new oral cancer treatment methods....

Aconiti lateralis Radix Praeparata inhibits Alzheimer’s disease by regulating the complex regulation network with the core of GRIN1 and MAPK1

Yutao Wang, Huixiang Zhang, Jing Wang, Ming Yu, Qianqian Zhang, Shan Yan, Dingyun You, Lanlan Shi, Lihuan Zhang, Limei Wang, Hongxiang Wu & Xue Cao
Current medicine for Alzheimer’s disease (AD) cannot effectively reverse or block nerve injury. Traditional Chinese Medicine practice and research imply Aconiti lateralis Radix Praeparata (Fuzi) may meet this goal. Analysing the anti-AD effect of Fuzi and its potential molecular mechanism. AD model cells were treated with Fuzi in 0-300 mg/mL for 24 h in 37 °C. The cell viability (CV) and length of cell projections (LCP) for each group were observed, analysed, and standardised using...

Logistics choices in a platform supply chain: A co-opetitive perspective

Pei Wang, Shaofu Du, Li Hu & Wenzhi Tang
With the popularity and development of the platform economy, numerous enterprises are choosing to cooperate with e-commerce platforms to expand online market, and the cooperation modes have exhibited innovation and diversification. This paper focuses on a new platform-based co-opetitive supply chain whereby a flagship store operated by the manufacturer (MFS) and a self-run store operated by the platform (PSS) coexist in the platform market. The manufacturer acts as the platform’s upstream contract manufacturer and downstream...

Fracture behavior of high-strength bolts and connections in the whole process of fire

Qiao Chen, Jian Jiang, Wei Chen & Jihong Ye
High-strength bolts have been widely used in steel bolted connections, and fire-induced high temperatures will affect its basic mechanical properties and fracture behavior, leading to failure of the connection and even collapse of the overall structure. Tensile experiments are conducted on Grade 10.9 high-strength bolts to investigate the effect of stress triaxiality and heating history on their mechanical and fracture properties in the whole process of fire. The true stress-strain curves are obtained to determine...

Numerical studies on post-fire residual capacity of axially restrained high strength steel columns under eccentric compression

Linxin Song & Guoqiang Li
For steel structures, steel columns are the critical load-bearing components and they are usually restrained by other components connected to them. At and after fire, the restraints will induce the deflection and stress in columns, and the mechanical properties of high strength steels may reduce significantly. These two aspects have further effects on the post-fire residual capacity of the restrained columns. This paper presents the details of numerical studies on post-fire residual capacity of axially...

Elective single versus double blastocyst-stage embryo transfer in women aged 36 years or older: a retrospective cohort study

He Cai, Ben Mol, Stephan Gordts, Hui Wang & Juanzi Shi
We evaluated if elective single-blastocyst transfer (eSBT) could be adopted in women aged 36 or older. In this retrospective cohort, women aged ≥36 years received IVF ovarian stimulation cycles and had ≥ two blastocysts. A total of 240 women underwent eSBT and 189 double-blastocyst transfer (DBT) in the first transfer cycle. The subsequent frozen-thawed embryo transfer cycles were a combination of single- and double- blastocyst transfers. Analysis was stratified for patients in age groups 36–37,...

Decreased echinocandin susceptibility in Candida parapsilosis causing candidemia and emergence of a pan-echinocandin resistant case in China

Yating Ning, Meng Xiao, David S. Perlin, Yanan Zhao, Minya Lu, Yi Li, Zhengyu Luo, Rongchen Dai, Shengjie Li, Jiajun Xu, Lingli Liu, Hong He, Yun Liu, Fushun Li, Yuguang Guo, Zhongju Chen, Yingchun Xu, Tianshu Sun & Li Zhang
Candida parapsilosis is becoming a predominant non-albicans cause of invasive candidiasis (IC). Echinocandins are the preferred choice for IC treatment and prophylaxis. Resistance to echinocandins in C. parapsilosis has emerged in several countries, but little is known about the susceptibility profile in China or about mechanisms of resistance. Here, we investigated the echinocandin susceptibilities of 2523 C. parapsilosis isolates collected from China and further explored the resistance mechanism among echinocandin-resistant isolates. Anidulafungin exhibited the highest...

Omicron variants escape the persistent SARS-CoV-2-specific antibody response in 2-year COVID-19 convalescents regardless of vaccination

Miao Wang, Bing Zhou, Qing Fan, Xinrong Zhou, Xuejiao Liao, Jingyan Lin, Zhenghua Ma, Jingke Dong, Haiyan Wang, Xiangyang Ge, Bin Ju & Zheng Zhang
With the ongoing COVID-19 pandemic and the emergence of various SARS-CoV-2 variants, a comprehensive evaluation of long-term efficacy of antibody response in convalescent individuals is urgently needed. Several longitudinal studies had reported the antibody dynamics after SARS-CoV-2 acute infection, but the follow-up was mostly limited to 1 year or 18 months at the maximum. In this study, we investigated the durability, potency, and susceptibility to immune evasion of SARS-CoV-2-specific antibody in COVID-19 convalescents for 2...

Bimetallic Mn-Ce loaded on different zeolite carriers applied in the toluene abatement in air by non–thermal plasma DDBD Reactor

Su Liu, Jiabin Zhou, Dan Liu, Xianjie Liu & Wenbo Liu
A sequence of zeolite carriers (Carrier = ZSM-5, Small crystal ZSM-5, MCM-41, SBA-15) were used to support active metals Mn-Ce, which have presented an enormous potential for plasma oxidation of toluene in air. The prepared samples were detected by means of N2 adsorption-desorption, SEM, XPS, H2-TPR, etc. Through the activity evaluation in the Non-thermal Plasma Reactor, we found that the catalysts with different carriers showed distinct degradation activities. The performance of mesoporous supported catalysts was...

Complete genome sequencing and comparative genomic analyses of a new spotted-fever Rickettsia heilongjiangensis strain B8

Maozhang He, Lifeng Zhang, Haoran Hu, Xiaohan Liu, Cong Zhang, Yu Xin, Boyu Liu, Zhen Chen, Kehan Xu & Yan Liu
Rickettsia heilongjiangensis, a tick-borne obligate intracellular bacterium and causative agent of spotted fever in China, has attracted increasing concern regarding its capability in causing human rickettsiosis. Here, we conducted a genomic analysis of a new Rickettsia heilongjiangensis strain B8 (B8) isolated from the serum of a patient who had been bitten by a Haemaphysalis longicornis tick in Anhui Province, China. The present study sought to identify exclusive genes that might be associated with the pathogenicity...

Structural insights into broadly neutralizing antibodies against SARS-CoV-2 elicited by hybrid immunity

Mengxiao Luo, Biao Zhou, Eswar R. Reddem, Bingjie Tang, Bohao Chen, Runhong Zhou, Hang Liu, Lihong Liu, Phinikoula S. Katsamba, Ka-Kit Au, Hiu-On Man, Kelvin Kai-Wang To, Kwok-Yung Yuen, Lawrence Shapiro, Shangyu Dang, David D Ho & Zhiwei Chen
Increasing spread by SARS-CoV-2 Omicron variants challenges existing vaccines and broadly reactive neutralizing antibodies (bNAbs) against COVID-19. Here we determine the diversity, potency, breadth and structural insights of bNAbs derived from memory B cells of BNT162b2-vaccinee after homogeneous Omicron BA.1 breakthrough infection. The infection activates diverse memory B cell clonotypes for generating potent class I/II or III bNAbs with new epitopes mapped to receptor-binding domain (RBD). The top eight bNAbs neutralize wildtype and BA.1 potently...

Different cellular mechanisms from low- and high-dose zinc oxide nanoparticles-induced heart tube malformation during embryogenesis

Mengwei Wang, Ping Zhang, Zeyu Li, Yu Yan, Xin Cheng, Guang Wang & Xuesong Yang
With the wide application of nanometer materials in daily life, people pay more attention to the potential toxicity of nanoparticles to human fetal development once the nanoparticles are absorbed into the human body during pregnancy. However, there was no directly solid evidence for ZnO NPs-caused congenital heart defects. Hence, we investigated the effect of ZnO NPs exposure on early cardiogenesis using the chicken/mouse embryo models. First, we showed ZnO NPs reduced H9c2 cell viability in...

Comparisons on fluid-solid heat transfer simulation for localized fire resistance

Xuanyi Zhou, Jinggang Zhou, Beihua Cong & Wei Wang
A research idea of multi-physics field coupling can be used to solve the structural fire resistance problem. Most of the previous CFD-FEM numerical simulations in structural fire resistance studies apply adiabatic surface temperature (AST) to accurately describe the complex fire analysis boundary conditions, and this methodology belongs to the use of iterative coupling methods to achieve fluid-solid heat transfer coupling. However, there is less application for another direct two-way coupling method. In this paper, a...

Clinical prognostic risk analysis and progression factor exploration of primary breast lymphoma

Jili Deng, Lan Mi, Xiaopei Wang, Jun Zhu, Chen Zhang & Yuqin Song
: A primary breast lymphomais a rare form of extranodal lymphoma type. We aimed to analyze prognosticriskfactors and explore relapse factors in primary breast diffuse large B cell lymphoma (PB-DLBCL). : From November 2003 to September 2020, sixty-three patients from two medical centers newly diagnosed with PB-DLBCL patients were analyzed retrospectively. : The median age was 52, and >50% of patients were post-menopausal. The international prognostics index (IPI) (0–1) was mainlyin the low-risk group (84%),...

Study on inhibition effect and mechanism of NH4H2PO4 on explosion of titanium powder

Wenjiao Dai, Xiangbao Meng, Shunxiang Yao, Jiqing Liu, Zhifeng Wang, Yang Liu, Panpan Yang & Fang Li
In order to effectively inhibit the explosion of titanium powder, this study used the Hartmann tube experimental system and the 20 L explosion tank experimental system to carry out flame propagation and explosion overpressure experiments, respectively, and the effect of NH4H2PO4 on the explosion suppression of titanium powder was studied. The results show that when the addition of NH4H2PO4 powder is 50%, the flame propagation velocity and explosion pressure can be reduced to the minimum,...

Study on inhibition effect and mechanism of NH4H2PO4 on explosion of titanium powder

Wenjiao Dai, Xiangbao Meng, Shunxiang Yao, Jiqing Liu, Zhifeng Wang, Yang Liu, Panpan Yang & Fang Li
In order to effectively inhibit the explosion of titanium powder, this study used the Hartmann tube experimental system and the 20 L explosion tank experimental system to carry out flame propagation and explosion overpressure experiments, respectively, and the effect of NH4H2PO4 on the explosion suppression of titanium powder was studied. The results show that when the addition of NH4H2PO4 powder is 50%, the flame propagation velocity and explosion pressure can be reduced to the minimum,...

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  • Shanghai Jiao Tong University
  • Chinese Academy of Medical Sciences & Peking Union Medical College
  • Zhejiang University
  • Fudan University
  • Sichuan University
  • Huazhong University of Science and Technology
  • University of Chinese Academy of Sciences
  • Capital Medical University
  • Sun Yat-sen University
  • Nanjing Medical University