57 Works

Corticosteroid prevents COVID-19 progression within its therapeutic window: a multicenter, proof-of-concept, observational study.

Yang Li, Xian Zhou, Tao Li, Shiji Chan, Yiqi Yu, Jing-Wen Ai, Haocheng Zhang, Feng Sun, Qiran Zhang, Lei Zhu, Lingyun Shao, Bin Xu & Wenhong Zhang
Background: Critically ill patients with COVID-19 are of grave concern. Those patients usually underwent a stage of excessive inflammation before developing acute respiratory distress syndrome. In this study, we test the hypothesis that short-term, low-to-moderate-dose corticosteroids would benefit patients when used in the early phase of excessive inflammation namely, the therapeutic window. Methods: Among a Shanghai cohort and a validation cohort, we enrolled COVID-19 patients showing marked radiographic progression. Short-term, low-to-moderate-dose corticosteroids were considered for...

Identification of mite-specific eosinophils in the colon of patients with ulcerative colitis

Shu-Wang Peng, Jiang-Ming Sheng, Bai-Sui Feng, Ke-Ping Peng, Gui-Xiang Tian, Cheng-Bai Liang, Ming-Hui Liu, Hai-Qing Xie, Qing Shu, Yan Li & Ping-Chang Yang
The pathogenesis of ulcerative colitis (UC) is unclear. House dust mite (HDM) is associated with immune inflammation in the body. This study is designed to identify the association between HDM and UC clinical symptoms. UC patients (n = 86) and non-UC control (NC) subjects (n = 64) were recruited. Colon lavage fluids (CLF) were collected from HDM skin prick test positive patients during colonoscopy, and analyzed by immunological approaches. HDM was detected in fecal samples,...

Getting the ROC into Sync

Liu Yang, Kajal Lahiri & Adrian Pagan
Judging the conformity of binary events in macroeconomics and finance has often been done with indices that measure synchronization. In recent years, the use of Receiver Operating Characteristic (ROC) curve has become popular for this task. This paper shows that the ROC and synchronization approaches are closely related, and each can be derived from a decision-making framework. Furthermore, the resulting global measures of the degree of conformity can be identified and estimated using the standard...

Transcriptomic analysis of the innate immune signatures of SARS-CoV-2 protein subunit vaccine ZF2001 and a mRNA vaccine RRV

Qian Wang, Ziyang Song, Jinghuan Yang, Qian He, Qunying Mao, Yu Bai, Jianyang Liu, Chaoqiang An, Xujia Yan, Bopei Cui, Lifang Song, Dong Liu, Miao Xu & Zhenglun Liang
Analysis of large-scale gene expression post vaccination can provide an overview of immune responses. We used transcriptional approaches to comprehensively analyze the innate immune response signatures elicited by protein subunit (PS) vaccine ZF2001 and a mRNA vaccine named RRV. A fine-grained time-dependent dissection of large-scale gene expression post immunization revealed that ZF001 induced MHC class II-related genes, including cd74 and H2-Aa, more expeditiously than RRV. Notably, RRV induced MHC class I-related genes like Tap1/2, B2m...

A tandem-repeat dimeric RBD protein-based COVID-19 vaccine ZF2001 protects mice and nonhuman primates

Yaling An, Shihua Li, Xiyue Jin, Jian-bao Han, Kun Xu, Senyu Xu, Yuxuan Han, Chuanyu Liu, Tianyi Zheng, Mei Liu, Mi Yang, Tian-zhang Song, Baoying Huang, Li Zhao, Wen Wang, A Ruhan, Yingjie Cheng, Changwei Wu, Enqi Huang, Shilong Yang, Gary Wong, Yuhai Bi, Changwen Ke, Wenjie Tan, Jinghua Yan … & George F. Gao
Safe, efficacious and deployable vaccines are urgently needed to control COVID-19 in the large-scale vaccination campaigns. We report here the preclinical studies of an approved protein subunit vaccine against COVID-19, ZF2001, which contains tandem-repeat dimeric receptor-binding domain (RBD) protein with alum-based adjuvant. We assessed vaccine immunogenicity and efficacy in both mice and non-human primates (NHPs). ZF2001 induced high levels of RBD-binding and SARS-CoV-2 neutralizing antibody in both mice and non-human primates, and elicited balanced TH1/TH2...

Experimental research about moisture transfer, burning behaviour and charring behaviour of glue laminated larch under fire heating using cone calorimeter

Anyang Sun, Kazunori Harada & Daisaku Nii
Cone calorimeter tests were conducted to investigate the moisture transfer, burning behaviour and charring behaviour of timber material under fire heating. 100 x 100 x 50mm glue laminated larch specimens were made in cross grain and straight grain directions. The initial surface moisture contents were 15.1 wt. % (cross grain specimens) and 13.5 wt.% (straight grain specimens). Twenty specimens were heated at 50kW/m2 of irradiance. The heating duration was varied between 10 to 50 minutes....

Structural basis of HCoV-19 fusion core and an effective inhibition peptide against virus entry

Huan Sun, Yan Li, Peipei Liu, Chengpeng Qiao, Xiaomin Wang, Lianao Wu, Kefang Liu, Yu Hu, Chao Su, Shuguang Tan, Shumei Zou, Guizhen Wu, Jinghua Yan, George Fu Gao, Jianxun Qi & Qihui Wang
Structural basis of HCoV-19 fusion core and an effective inhibition peptide against virus entry

Nickel (ii) effects on Anammox reaction: reactor performance, dehydrogenase, sludge morphology and microbial community changes

Jian Cheng, Liang Qiao, Wen Xu, Yulan Qian, Yiyang Ge, Ting Xia & Yan Li
Nickel (ii) (Ni2+) is considered as one of the necessary trace elements in the process of Anammox culture, but it may have toxic effects at high concentration. This study explored the long-term influence of Ni2+ on the denitrification efficiency of Anammox bioreactors. The results showed that when the concentration of Ni2+ was 0.5 mg/L, the bioreactor had the highest denitrification efficiency, while the removal efficiency of ammonia nitrogen and nitrite nitrogen gradually decreased at concentrations...

A novel viral vaccine platform based on engineered transfer RNA

Tong-Yun Wang, Fan-Dan Meng, Guo-Ju Sang, Hong-Liang Zhang, Zhi-Jun Tian, Hao Zheng, Xue-Hui Cai & Yan-Dong Tang
In recent years, an increasing number of emerging and remerging virus outbreaks have occurred and the rapid development of vaccines against these viruses has been crucial. Controlling the replication of premature termination codon (PTC)-containing viruses is a promising approach to generate live but replication-defective viruses that can be used for potent vaccines. Here, we used anticodon-engineered transfer RNAs (ACE-tRNAs) as powerful precision switches to control the replication of PTC-containing viruses. We showed that ACE-tRNAs display...

Locating and sizing tumor nodules in human prostate using instrumented probing – computational framework and experimental validation

Antonio Candito, Daniel W. Good, Javier Palacio-Torralba, Steven Hammer, Olufemi Johnson, S. Alan McNeill, Robert L. Reuben & Yuhang Chen
Detection of tumor nodules is key to early cancer diagnosis. This study investigates the potential of using the mechanical data, acquired from probing the prostate for detecting the existence, and, more importantly, characterizing the size and depth, from the posterior surface, of the prostate cancer (PCa) nodules. A computational approach is developed to quantify the uncertainty of nodule detectability and is based on identifying stiffness anomalies in the profiles of point force measurements across transverse...

Facile in situ fabrication of a direct Z-scheme BiOCl/bismuth niobate heterojunction and its effective photodegradation of RhB

Huining Zhang, Yankui Xiao, Zhongyu Shi, Lihong Tian, Yuling Tang, Xingmao Liu, Yangyi Tian & Yan Lin
Bismuth-based photocatalytic technology has been a promising way to degrade contaminants in the aqueous system. In this work, a direct Z-scheme BiOCl/Bi3NbO7 heterojunction with a different molar ratio of Bi and Nb elements was fabricated through a facile in situ growth method. The structural and photoelectric characteristics of these as-prepared samples were investigated by SEM, XPS, TEM, XRD, BET, UV-vis DRS, PL, EIS and TPC. The photocatalytic activity was evaluated by the degradation of RhB,...

The causal relationship between Thyroid function and Lung cancer: A mendelian randomization study

Xinhui Wang, Xue Liu, Jie Yuan, Huizhi Zhou, Yuyao Wang, Guoyu Tian, Xueying Liu, Mulin Tang, Xue Meng, Yuchen Li, Yuwei Chai, Chunjia Kou, Qingqing Yang, Juyi Li, Li Zhang & Haiqing Zhang
The role of thyroid hormones in tumors has been discussed in observational studies; however, the causal relationship between them remains controversial. Six different Mendelian randomization (MR) Methods, including Inverse variance weighted (IVW), MR-Egger, Simple mode, MR-Pleiotropy Residual Sum and Outlier methods (MR-PRESSO), Weighted mode and Weighted median were used to Two-Sample MR analysis. Our research shows that hypothyroidism genetically has a protective causal association with lung cancer.

SWATH Proteomics analysis of placental tissue with intrahepatic cholestasis of pregnancy_part2

Yuxuan Jiang, Xiaoping Yin, Qian Xu, Xiaoxiao Tang, Huajie Zhang, Xueshan Cao, Jing Lin, Yi Wang, Fei Yang, NaseerUllah Khan, Liming Shen & Danqing Zhao
Introduction: Intrahepatic cholestasis of pregnancy (ICP) usually occurs in the second and third trimesters. The disease's etiology and diagnostic criteria are currently unknown. Based on a sequence window to obtain all theoretical fragment ions (SWATH) proteomic approach, this study sought to identify potential proteins in placental tissue that may be involved in the pathogenesis of ICP and adverse fetal pregnancy outcomes. Methods: The postpartum placental tissue of pregnant women with ICP were chosen as the...

SWATH Proteomics analysis of placental tissue with intrahepatic cholestasis of pregnancy_part2

Yuxuan Jiang, Xiaoping Yin, Qian Xu, Xiaoxiao Tang, Huajie Zhang, Xueshan Cao, Jing Lin, Yi Wang, Fei Yang, NaseerUllah Khan, Liming Shen & Danqing Zhao
Introduction: Intrahepatic cholestasis of pregnancy (ICP) usually occurs in the second and third trimesters. The disease's etiology and diagnostic criteria are currently unknown. Based on a sequence window to obtain all theoretical fragment ions (SWATH) proteomic approach, this study sought to identify potential proteins in placental tissue that may be involved in the pathogenesis of ICP and adverse fetal pregnancy outcomes. Methods: The postpartum placental tissue of pregnant women with ICP were chosen as the...

Heterologous Omicron-adapted vaccine as a secondary booster promotes neutralizing antibodies against Omicron and its sub-lineages in mice

Jianyang Liu, Qian He, Fan Gao, Lianlian Bian, Qian Wang, Chaoqiang An, Lifang Song, Jialu Zhang, Dong Liu, Ziyang Song, Lu Li, Yu Bai, Zhongfang Wang, Zhenglun Liang, Qunying Mao & Miao Xu
Over one billion people have received 2–3 dosages of an inactivated COVID-19 vaccine for basic immunization. Whether a booster dose should be delivered to protect against the Omicron variant and its sub-lineages, remains controversial. Here, we tested different vaccine platforms targeting the ancestral or Omicron strain as a secondary booster of the ancestral inactivated vaccine in mice. We found that the Omicron-adapted inactivated viral vaccine promoted a neutralizing antibody response against Omicron in mice. Furthermore,...

Interactions between host epithelial cells and Acinetobacter baumannii promote the emergence of highly antibiotic resistant and highly mucoid strains

Wang Zhang, Yue Yao, Hua Zhou, Jintao He, Jingfen Wang, Li Li, Minsong Gao, Xiaochen Liu, Ya Shi, Jinzhong Lin, Jianzhao Liu, Huan Chen, Yu Feng, Zhihui Zhou, Yunsong Yu & Xiaoting Hua
Acinetobacter baumannii is an important nosocomial pathogen. Upon colonizing a host, A. baumannii are subjected to selective pressure by immune defenses as they adapt to the host environment. However, the mechanism of this pathoadaptation is unknown. Here, we established an in vitro system to evolve A. baumannii driven by the continuous selective pressure exerted by epithelial cells, and we used a combination of experimental evolution, phenotypic characterization and multi-omics analysis to address the underlying mechanism....

Complement receptor 3 mediates Aspergillus fumigatus internalization into alveolar epithelial cells with the increase of intracellular phosphatidic acid by activating FAK

Xuelin Han, Xueting Su, Zhiqian Li, Yanxi Liu, Shuo Wang, Miao Zhu, Changjian Zhang, Fan Yang, Jingya Zhao, Xianping Li, Fangyan Chen & Li Han
Complement receptor 3 (CD11b/CD18) is an important receptor that mediates adhesion, phagocytosis and chemotaxis in various immunocytes. The conidia of the medically-important pathogenic fungus, Aspergillus fumigatus can be internalized into alveolar epithelial cells to disseminate its infection in immunocompromised host; however, the role of CR3 in this process is poorly understood. In the present study, we investigated the potential role of CR3 on A. fumigatus internalization into type II alveolar epithelial cells and its effect...

Transcriptional Regulation of the Chicken ASMT Gene- A Preliminary Analysis

Jun Teng, Dan Wang, Wenwen Wang, Chao Ning, Qin Zhang & Hui Tang
1. Melatonin is an indole hormone that, among its myriad biological functions, regulates circadian and seasonal rhythms in animals. The ASMT gene plays an essential role in melatonin synthesis. However, in chickens, little is known about the regulatory elements governing its transcription. 2. The following study identified the transcription start site of the chicken ASMT gene by 5’-RACE. Then, the proximal minimal promoter was identified using a series of 5′ truncations of the ASMT promoter...

SDH5 down-regulation mitigates the damage of osteoporosis via inhibiting the MyD88/NF-κB signaling pathway

Hongzi Wu, Dehua Zhang, Haijun Xia, Yongqi Li, Feng Mao & Yi Liao
Osteoporosis has become a serious public health problem especially in postmenopausal women. This work aims to assess both the function and mechanism of SDH5 in osteoporosis. The animal model of osteoporosis in sprague-dawley rats was established by ultilizing Ovariectomy (OVX). The trabecular bone morphometry had been determined by micro-CT, and tibia injury of rats was detected through HE and alcian blue staining. Meanwhile, the levels of oxidative stress factors, including MDA, CAT, GSH-Px, and SOD,...

Cell membrane-anchored anti-HIV single-chain antibodies and bifunctional inhibitors targeting the gp41 fusion protein: new strategies for HIV gene therapy

Yue Chen, Hongliang Jin, Xiaoran Tang, Li Li, Xiuzhu Geng, Yuanmei Zhu, Huihui Chong & Yuxian He
Emerging studies indicate that infusion of HIV-resistant cells could be an effective strategy to achieve a sterilizing or functional cure. We recently reported that glycosylphosphatidylinositol (GPI)-anchored nanobody or a fusion inhibitory peptide can render modified cells resistant to HIV-1 infection. In this study, we comprehensively characterized a panel of newly isolated HIV-1-neutralizing antibodies as GPI-anchored inhibitors. Fusion genes encoding the single-chain variable fragment (scFv) of 3BNC117, N6, PGT126, PGT128, 10E8, or 35O22 were constructed with...

Burning behaviour of GLT walls during cooling period after ISO 834 exposure in a small furnace

Kazunori Harada, Anyang Sun, Shoma Makino & Daisaku Nii
Fire resistance of timber structures is mainly investigated by ISO 834 standard fire resistance tests. In the tests, specimens are heated in accordance with prescribed standard time-temperature curve and the time to failure is measured, but no information is available with regard to the behaviours during post-heating period. In case of timber constructions, post-heating behaviour is important because the self-burning continues during cooing period. To investigate the behaviour during cooling period, fire resistance tests of...

Reuse of composted rural food waste in China as vermicomposting substrate: Effects on earthworms, associated microorganisms, and economic benefits

Huabao Zheng, Min Wang, Yueqin Fan, Jian Yang, Zhuoqun Zhao, Hengyuan Chen, Zhenwei Ye, Zhanwang Zheng & Kefei Yu
Aerobic composting of rural food waste (FW) in China using a composting device results in a substantial financial burden on the government. This study aimed to assess the feasibility of mitigating this cost using vermicomposting of composted FW. The specific aims were to elucidate the effects of composted FW on earthworm growth and reproduction, reveal the changes in the physical and chemical properties of earthworm casts during vermicomposting, identify the microbial community structure associated with...

Development of a naringenin microemulsion as a prospective ophthalmic delivery system for the treatment of corneal neovascularization: in vitro and in vivo evaluation

Yu Ma, Jingjing Yang, Yali Zhang, Chunyan Zheng, Zhen Liang, Ping Lu, Fei Song, Yuwei Wang & Junjie Zhang
Naringenin, a flavonoid, possesses antiangiogenic potential and inhibits corneal neovascularization (CNV); however, its therapeutic use is restricted due to poor solubility and limited bioavailability. In this study, we developed a naringenin microemulsion (NAR-ME) for inhibiting CNV. NAR-ME formulation was composed of triacetin (oil phase), Cremophor RH40 (CRH40), PEG400, and water, its droplet size was 13.22 ± 0.13 nm with a narrow size distribution (0.112 ± 0.0014). The results demonstrated that NAR-ME released higher and permeated...

Sevelamer arsenite nanoparticle as a Pi-responsive drug carrier and embolic agent for chemoembolization

Qiu-Chen Bi, Jian-Jun Tang, Jun Zhao, Yang-Feng Lv, Zhi-Qiang Deng, Hong Chen, Yu-Hua Xu, Chuan-Sheng Xie, Qing-Rong Liang, Rong-Guang Luo & Qun Tang
Arsenic trioxide (As2O3, ATO) has limited therapeutic benefit to treat solid tumors, whether used alone or in combination. Nanoscale drug delivery vehicles have great potential to overcome the limitation of the utility of ATO by rapid renal clearance and dose-limiting toxicity. Polymeric materials ranging from gelatin foam to synthetic polymers such as poly(vinyl alcohol) were developed for vascular embolic or chemoembolic applications. Recently, we have introduced sevelamer, an oral phosphate binder, as a new polymeric...

The demulsification of oily wastewater by a hyperbranched polymer grafted SiO2

Yu Zhang, Jiazhe Kuang, Bin Li, Yuanzhu Mi, Ying Yang & Xuening Feng
In this work, a PAMAM@SiO2 demulsifier was synthesized by the reaction of a hyperbranched poly (amido amine) (PAMAM) and KH570 modified SiO2. The structure, morphology and composition of PAMAM@SiO2 were characterized by FT-IR, SEM and EDS. The effect of the concentration of PAMAM@SiO2, salinity and temperature on the demulsification performance was systematically studied. The results showed that the transmittance of aqueous phase after demulsification could reach as high as 92.1% at optimal conditions, and corresponding...

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  • Sichuan Agricultural University
  • Zhejiang University
  • Fudan University
  • Capital Medical University
  • Chinese Academy of Medical Sciences & Peking Union Medical College
  • Shanghai Jiao Tong University
  • Shandong University
  • Sun Yat-sen University
  • Sichuan University
  • Tongji University