18 Works

Comparable antigen-specific T cell responses in vaccinees with diverse humoral immune responses after primary and booster BBIBP-CorV vaccination

Dong Wei, Yingying Chen, Xiaoqi Yu, Yang-dian Lai, Wenxin Xu, Ping Ji, Zhitao Yang, Erzhen Chen, Xinxin Zhang & Ying Wang
BBIBP-CorV exerts efficient protection against SARS-CoV-2 infection. However, waning vaccine-induced humoral immune responses after two-dose vaccination has significantly undermined durable immuno-protection. In this study, we have demonstrated that although anti-spike (S) antibody responses in BBIBP-CorV vaccinees exhibited three serotypes after 6 months including de novo sero-negative, sero-positive and sero-decay features, S-specific interferon-γ release as well as Th1 cytokine production in CD4+ and CD8+ T cells were comparable, especially in vaccinees without detectable neutralizing antibodies. Notably,...

Effect of ocular magnification on macular choroidal thickness measurements made using optical coherence tomography in children

Junjie Deng, Jiali Jin, Bo Zhang, Siqi Zhang, Jingjing Wang, Shuyu Xiong, Tianyu Cheng, Kun Liu, Jiannan Huang, Xiangui He & Xun Xu
To evaluate the relationship between ocular magnification correction and macular choroidal thickness (ChT) measurements in children, and to demonstrate when ocular magnification correction is necessary. Chinese children aged 6–9 years with various refractive statuses were included. Swept-source optical coherence tomography was used to measure macular ChT. A self-designed program was adopted to simulate ChT changes in each sector of the ETDRS grid in the macula under various simulated axial lengths (ALs). ChT measurements were not...

Transcriptional Regulation of the Chicken ASMT Gene- A Preliminary Analysis

Jun Teng, Dan Wang, Wenwen Wang, Chao Ning, Qin Zhang & Hui Tang
1. Melatonin is an indole hormone that, among its myriad biological functions, regulates circadian and seasonal rhythms in animals. The ASMT gene plays an essential role in melatonin synthesis. However, in chickens, little is known about the regulatory elements governing its transcription. 2. The following study identified the transcription start site of the chicken ASMT gene by 5’-RACE. Then, the proximal minimal promoter was identified using a series of 5′ truncations of the ASMT promoter...

Molecular detection and identification of relapsing fever Borrelia in ticks and wild small mammals in China

Xiao-Ai Zhang, Feng Tian, Yue Li, Xiao-Long Zhang, Bao-Gui Jiang, Bao-Cheng Liu, Jing-Tao Zhang, Shen Tian, Heng Ding, Shuang Li, Hao Li, Li-Qun Fang & Wei Liu
We identified relapsing fever (RF) Borrelia in 1.45% (145/10426) of the ticks and 1.40% (40/2850) of the wild mammals in a field investigation in China. Three RF Borrelia species, including human-pathogenic Borrelia miyamotoi, Borrelia persica and unclassified Babesia sp. were determined. Main species determined from ticks was B. miyamotoi (44.14%), followed by the unclassified Borrelia sp. (42.76%), and Borrelia theileri (13.10%). In wild mammals, main species found was B. persica (57.50%), followed by the unclassified...

RT-LAMP assay for rapid detection of the R203M mutation in SARS-CoV-2 Delta variant

Jianing Yang, Xuejiao Hu, Wenzhuo Wang, Yujing Yang, Xinqiang Zhang, Wei Fang, Lei Zhang, Shan Li & Bing Gu
The highly infectious Delta variant strain of SARS-CoV-2 remains globally dominant and undermines COVID-19 vaccines. Rapid detection of the Delta variant is crucial for the identification and quarantine of infected individuals. In this study, our aim was to design and validate a genotyping RT-LAMP method to detect Delta variants specifically. R203M in the N gene of SARS-CoV-2 was chosen as the Delta variant-specific mutation for genotyping. To target the R203M-harboring region and the conserved sequence...

Efficacy of heterologous boosting against SARS-CoV-2 using a recombinant interferon-armed fusion protein vaccine (V-01): a randomized, double-blind and placebo-controlled phase III trial

Xuan-Yi Wang, Syed Faisal Mahmood, Fang Jin, Wee Kooi Cheah, Muhammad Ahmad, Mian Amjad Sohail, Waheed Ahmad, Vijaya K Suppan, Muneeba Ahsan Sayeed, Shobha Luxmi, Aik-Howe Teo, Li Yuan Lee, Yang-Yang Qi, Rong-Juan Pei, Wei Deng, Zhong-Hui Xu, Jia-Ming Yang, Yan Zhang, Wu-Xiang Guan & Xiong Yu
Waning of neutralizing titers along with decline of protection efficacy after the second dose of COVID-19 vaccines was observed, including China-made inactivated vaccines. Efficacy of a heterologous boosting using one dose of a recombinant SARS-CoV-2 fusion protein vaccine (V-01) in inactivated vaccine-primed population was studied, aimed to restore the immunity. A randomized, double-blind and placebo-controlled phase Ⅲ trial was conducted in healthy people aged 18 years or older in Pakistan and Malaysia. Each eligible participant...

Healthy Outcomes of Patients with COVID-19 Two Years after the Infection: A Prospective Cohort Study

Dapeng Li, Xuejiao Liao, Zhi Liu, Zhenghua Ma, Jingke Dong, Guoqin Zheng, Mei Zi, Fang Wang, Qing He, Guobao Li, Zheng Zhang & Lei Liu
The long-term effect of coronavirus disease 2019 (COVID-19) has been rarely known. This study aimed to investigate healthy outcomes of COVID-19 survivors up to 2 years after the infection. A total of 155 COVID-19 patients, who were discharged from Shenzhen Third People's Hospital from February 2020 to April 2020, were enrolled and followed up until March 4, 2022. COVID-19 survivors received questionnaires of long COVID symptoms and psychological symptoms, pulmonary function tests, chest computed tomography...

Identification of mite-specific eosinophils in the colon of patients with ulcerative colitis

Shu-Wang Peng, Jiang-Ming Sheng, Bai-Sui Feng, Ke-Ping Peng, Gui-Xiang Tian, Cheng-Bai Liang, Ming-Hui Liu, Hai-Qing Xie, Qing Shu, Yan Li & Ping-Chang Yang
The pathogenesis of ulcerative colitis (UC) is unclear. House dust mite (HDM) is associated with immune inflammation in the body. This study is designed to identify the association between HDM and UC clinical symptoms. UC patients (n = 86) and non-UC control (NC) subjects (n = 64) were recruited. Colon lavage fluids (CLF) were collected from HDM skin prick test positive patients during colonoscopy, and analyzed by immunological approaches. HDM was detected in fecal samples,...

Decreased echinocandin susceptibility in Candida parapsilosis causing candidemia and emergence of a pan-echinocandin resistant case in China

Yating Ning, Meng Xiao, David S. Perlin, Yanan Zhao, Minya Lu, Yi Li, Zhengyu Luo, Rongchen Dai, Shengjie Li, Jiajun Xu, Lingli Liu, Hong He, Yun Liu, Fushun Li, Yuguang Guo, Zhongju Chen, Yingchun Xu, Tianshu Sun & Li Zhang
Candida parapsilosis is becoming a predominant non-albicans cause of invasive candidiasis (IC). Echinocandins are the preferred choice for IC treatment and prophylaxis. Resistance to echinocandins in C. parapsilosis has emerged in several countries, but little is known about the susceptibility profile in China or about mechanisms of resistance. Here, we investigated the echinocandin susceptibilities of 2523 C. parapsilosis isolates collected from China and further explored the resistance mechanism among echinocandin-resistant isolates. Anidulafungin exhibited the highest...

Omicron infections profile and vaccination status among 1881 liver transplant recipients: a multi-center retrospective cohort

Ensi Ma, Jingwen Ai, Yi Zhang, Jianming Zheng, Xiaogang Gao, Junming Xu, Hao Yin, Zhiren Fu, Hao Xing, Li Li, Liying Sun, Heyu Huang, Quanbao Zhang, Linlin Xu, Yanting Jin, Rui Chen, Guoyue Lv, Zhijun Zhu, Wenhong Zhang & Zhengxin Wang
A wave of Omicron infections rapidly emerged in China in 2022, but large-scale data concerning the safety profile of vaccines and Coronavirus disease 2019 (COVID-19) infection features in liver transplant (LT) recipients have not been collected. Therefore, the aim of this study was to assess the protectiveness and safety profile of the inactivated vaccines in LT patients against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron variant infections. A multi-center retrospective study was conducted in...

Booster vaccination is required to elicit and maintain COVID-19 vaccine-induced immunity in SIV-infected macaques

Pingchao Li, Qian Wang, Yizi He, Chenchen Yang, Zhengyuan Zhang, Zijian Liu, Bo Liu, Li Yin, Yilan Cui, Peiyu Hu, Yichu Liu, Pingqian Zheng, Wei Wang, Linbing Qu, Caijun Sun, Suhua Guan, Liqiang Feng & Ling Chen
Prolonged infection and possible evolution of SARS-CoV-2 in patients living with uncontrolled HIV-1 infection highlight the importance of an effective vaccination regimen, yet the immunogenicity of COVID-19 vaccines and predictive immune biomarkers have not been well investigated. Herein, we report that the magnitude and persistence of antibody and cell-mediated immunity (CMI) elicited by an Ad5-vectored COVID-19 vaccine are impaired in SIV-infected macaques with high viral loads (> 105 genome copies per ml plasma, SIVhi) but...

Effect of acute acidic stress on survival and metabolic activity of zooplankton from Lake Biwa, Japan

Huanan Gao, Xin Liu & Syuhei Ban
Acute acidic stress from sources such as acid rain might impact on lake ecosystems in many aspects. Because zooplankton is sensitive to environmental change, it could be a good biotic indicator of the effects of acidification. We investigated survival and metabolic rates of three dominant zooplankton taxa in Lake Biwa, the cladoceran Daphnia pulicaria, and the copepods Eodiaptomus japonicus and Cyclopoida spp., when subjected to different pH values (pH 4.0−8.0) at low (10 °C) and...

Batch-to-batch consistency trial of an adenovirus type-5 vector-based COVID-19 vaccine in adults aged 18 years and above

Zhuo-Pei Li, Yun-Feng Shi, Li-Hua Hou, Peng-Fei Jin, Shi-Hua Ma, Hong-Xing Pan, Jin-Long Zhang, Yong-Mei Shan, Hai-Tao Huang, Shi-Po Wu, Pan Du, Xue Wang, Li-Li Wang, Rui-Jie Wang, Ying Wang, Xue-Wen Wang, Feng-Cai Zhu & Jing-Xin Li
The demonstration of batch-to-batch consistency is indispensable for quality control of vaccines. We did a randomized, double-blind, parallel-controlled trial to evaluate the immunogenicity consistency of one shot of Ad5-nCoV in healthy adults who had not received any COVID-19 vaccine before. All eligible participants were randomly assigned equally to receive one of three consecutive batches of Ad5-nCoV (5×1010 viral particles/vial, 0.5 mL). The primary endpoint was geometric mean titers (GMTs) of serum SARS-CoV-2 receptor binding domain...

Identification of mite-specific eosinophils in the colon of patients with ulcerative colitis

Shu-Wang Peng, Jiang-Ming Sheng, Bai-Sui Feng, Ke-Ping Peng, Gui-Xiang Tian, Cheng-Bai Liang, Ming-Hui Liu, Hai-Qing Xie, Qing Shu, Yan Li & Ping-Chang Yang
The pathogenesis of ulcerative colitis (UC) is unclear. House dust mite (HDM) is associated with immune inflammation in the body. This study is designed to identify the association between HDM and UC clinical symptoms. UC patients (n = 86) and non-UC control (NC) subjects (n = 64) were recruited. Colon lavage fluids (CLF) were collected from HDM skin prick test positive patients during colonoscopy, and analyzed by immunological approaches. HDM was detected in fecal samples,...

Cadomian crust of Eastern Iran: evidence from the Tapeh Tagh granitic gneisses

Habib Mollai, Rahim Dabiri, Habib Allah Torshizian, Georgia Pe-Piper & Wei Wang
Late Neoproterozoic to Early Cambrian (Cadomian) magmatic rocks are abundant in Iran, but their occurrence in east-central Iran is voluminous. This paper reports, for the first time, on the Cadomian rocks of the eastern Iranian region known as Tapeh Tagh and deals with their geochemistry and uranium-lead (U-Pb) zircon ages. The Tapeh Tagh region contains masses of Cadomian meta-igneous rocks (including various types of granitic gneiss) and overlying meta-sedimentary rocks. The granitic gneisses show tectonic...

Genetic, biological and epidemiological study on a cluster of H9N2 avian influenza virus infections among chickens, a pet cat, and humans at a backyard farm in Guangxi, China

Jing Yang, Jianhua Yan, Cheng Zhang, Shanqin Li, Manhua Yuan, Chunge Zhang, Chenguang Shen, Yang Yang, Lifeng Fu, Guanlong Xu, Weifeng Shi, Zhenghai Ma, Ting Rong Luo & Yuhai Bi
During an investigation in October 2018, two people with diarrhoea, mild abdominal pain, and mild arthralgia symptoms in Guangxi, China, were identified as infected by H9N2 avian influenza virus (AIV). Four H9N2 AIVs were isolated from one of two patients, a pet cat, and a dead chicken (two respective isolates from its lung and kidney tissues) bred by the patients at a backyard farm. Epidemiological investigation indicated that the newly bought chicken died first, and...

Human parainfluenza 3 and respiratory syncytial viruses detected in pangolins

Tengcheng Que, Jing Li, Yugan He, Panyu Chen, Wei Lin, Meihong He, Lei Yu, Aiqiong Wu, Luohao Tan, Yingjiao Li, Yanling Hu & Yigang Tong
Pangolins have gained increasing global attention owing to their public health significance as potential zoonotic hosts since the identification of SARS-CoV-2-related viruses in them. Moreover, these animals could carry other respiratory viruses. In this study, we investigated the virome composition of 16 pangolins that died in 2018 with symptoms of pneumonia using metagenomic approaches. A total of eight whole virus sequences belonging to the Paramyxoviridae or Pneumoviridae families were identified, including one human parainfluenza virus...

Antibody persistence and safety after heterologous boosting with orally aerosolised Ad5-nCoV in individuals primed with two-dose CoronaVac previously: twelve-month analyses of a randomized controlled trial

Lairun Jin, Rong Tang, Shipo Wu, Xiling Guo, Haitao Huang, Lihua Hou, Xiaoqin Chen, Tao Zhu, Jinbo Gou, Jin Zhong, Hongxing Pan, Lunbiao Cui, Yin Chen, Xin Xia, Jialu Feng, Xue Wang, Qi Zhao, XiaoYu Xu, Zhuopei Li, Xiaoyin Zhang, Wei Chen, Jingxin Li & Fengcai Zhu
Antibody persistence and safety up to 12 months of heterologous orally administered adenovirus type-5 vector-based COVID-19 vaccine (Ad5-nCoV) in individuals who were primed with two-dose inactivated SARS-CoV-2 vaccine (CoronaVac) previously, has not been reported yet. This randomized, open label, single-center trial included Chinese adults who have received two-dose CoronaVac randomized to low-dose or high-dose aerosolised Ad5-nCoV group, or CoronaVac group. In this report, we mainly evaluated the geometric mean titres (GMTs) of neutralising antibodies (NAbs)...

Registration Year

  • 2022

Resource Types

  • Other


  • Chinese Academy of Medical Sciences & Peking Union Medical College
  • Xuzhou Medical College
  • Zhejiang University
  • Fudan University
  • Shanghai Jiao Tong University
  • Huazhong University of Science and Technology
  • Chinese Academy of Agricultural Sciences
  • Sichuan University
  • China Agricultural University
  • West China Hospital of Sichuan University