This study aimed to assess the consequences of reducing the concentrates supply level (CSL) in the lactating cows’ diet on Alpine dairy system’s GHG emissions. Consequential-based Life Cycle Assessment (cLCA) was adopted to assess the consequences within the ‘dairy_system’ (farm plus milk processing) and outside (‘expanded_system’). The functional unit was 1 kg of protein and fat (ProtFat). Data (1-year average) originated from 40 dairy farms in the Alps, collected through farm questionnaires during farm visits....

Additional file 10: Figure S10. Inhibition of transforming growth factor beta (TGF-β) signaling blocks LAMC2-induced metastasis. (a) Enrichment plots for pancreatic cancer, focal adhesion, hypoxia, MAPK signaling, glycolysis and gluconeogenesis pathways in EGFP+ versus EGFP- cells isolated by FACS from subcutaneous tumors. (b) Western blot analysis for pSMAD2 and SMAD2 in EGFP- versus EGFP+ PANC-1 and #253 cells. Parallel GAPDH immunoblotting was performed. (c) Flow cytometry quantification of EGFP and ALK5-TGFβR1 in EGFP- and EGFP+...

Additional file 1: Regression analyses and English version of the Social Relationship Coping Efficacy Scale.

Additional file 1: Regression analyses and English version of the Social Relationship Coping Efficacy Scale.

Supplemental material, sj-docx-2-tai-10.1177_20499361221141771 for The impact of Antimicrobial Stewardship Programmes in paediatric emergency department and primary care: a systematic review by Giulia Brigadoi, Sara Rossin, Davide Visentin, Elisa Barbieri, Carlo Giaquinto, Liviana Da Dalt and Daniele Donà in Therapeutic Advances in Infectious Disease

Supplemental material, sj-docx-4-tai-10.1177_20499361221141771 for The impact of Antimicrobial Stewardship Programmes in paediatric emergency department and primary care: a systematic review by Giulia Brigadoi, Sara Rossin, Davide Visentin, Elisa Barbieri, Carlo Giaquinto, Liviana Da Dalt and Daniele Donà in Therapeutic Advances in Infectious Disease

Many primary tumours have low levels of molecular oxygen (hypoxia), and hypoxic tumours respond poorly to therapy. Pan-cancer molecular hallmarks of tumour hypoxia remain poorly understood, with limited comprehension of its associations with specific mutational processes, non-coding driver genes and evolutionary features. Here, as part of the ICGC/TCGA Pan-Cancer Analysis of Whole Genomes (PCAWG) Consortium, which aggregated whole genome sequencing data from 2658 cancers across 38 tumour types, we quantify hypoxia in 1188 tumours spanning...

Additional file 8: Figure S8. Characterization of human LAMC2-EGFP PDAC cells in vitro and in vivo. (a) Representative images of EGFP+ and EGFP-cells grown in Matrigel. (b) Organoid formation capacity of EGFP+ and EGFP-cells. (c) Quantification of organoid size of EGFP+ and EGFP-cells. (d) qPCR analysis for LAMC2, EMT, MMP2 and MMP10 gene expression in EGFP+ and EGFP-cells. Data are normalized to GAPDH and are presented as fold change in gene expression relative to the...

Additional file 2: Figure S2. Increased LAMC2 expression is associated with aggressive signature. (a) Dimensional reduction plot (DimPlot) of multiple cell types identified in PDAC primary tumors by single-cell RNA sequencing (scRNA-Seq). The clusters are color-coded based on cell types identified using known cell type-specific markers and are visualized using t-Distributed Stochastic Neighbor embedding (t-SNE). (b) Violin plot showing expression levels of LAMC2 in stromal versus ductal (tumor) cells identified in PDAC primary tumors by...

Additional file 5: Figure S5. Loss of LAMC2 reduces stemness. (a) Flow cytometry for apoptotic cells as determined by AnnexinV/PI staining in control and LAMC2 knockdown cells. (b) Flow cytometry quantification of CD44 in sh empty and LAMC2 knockdown cells. (c) qPCR analysis of CD133 in sh empty and LAMC2 knockdown cells. Data are normalized to GAPDH and are presented as fold change in gene expression relative to sh empty. (d) Flow cytometry analysis of...

Supplemental material, sj-docx-1-jbm-10.1177_03936155231154663 for Overordering of tumor marker for outpatients revealed by performance indicators and the impact of a health policy intervention: An observational study using administrative records by Massimo Gion, Roberto De Gobbi, Manuel Zorzi, Giovanni Carretta, Luca Leonardi, Stefano Guzzinati, Chiara Trevisiol, Maurizio Cancian, Giulia Cardinali, Federica Michieletto, Ruggero Dittadi, Aline S.C. Fabricio, Massimo Rugge and Francesca Russo in The International Journal of Biological Markers

Background: Recent evidence showed adverse mental health outcomes associated with the COVID-19 pandemic, including trauma-related symptoms. The Global Psychotrauma Screen (GPS) is a brief instrument designed to assess a broad range of trauma-related symptoms with no available validation in the Italian population. Aims: This study aimed to examine the factor structure of the Italian version of the GPS in a general population sample exposed to the COVID-19 pandemic and to evaluate trauma-related symptoms in the...

Appendix 1

Supplemental Table

The traditional tale, which later became a legend, told of mysterious footprints imprinted “in the rock” or “in the lava”, left by a mysterious, supernatural being, perhaps “the devil”, who was able to walk on the burning volcanic deposit without damage. Over time, this legend has been gradually replaced first by a series of unfounded assumptions and finally by an even more fascinating scientific reality: the “Devil's Trails” (“Ciampate del diavolo”) are fossilized footprints of...

A first determination of the value of the annual growth factor (0.766 mm/yr) of Aspicilia cinerea has been done through the application of a lichenometric system calibrated in the surroundings of the “Devil’s Trails” palaeontological site (Roccamonfina volcano). The application of this parameter to some samples within this site allowed researchers to know that the re-exposure of the surface preserving fossil human and animal footprints did not start before the early 1800s. This seems perfectly...

The world-famous Roccamonfina site represents one among the very few opportunities to investigate about the evolution of bipedal locomotion of Middle Pleistocene humans. The site includes at least 80, finely preserved human footprints in anatomical position, divided into four trackways. We applied a biomechanical model to verify if the Roccamonfina’s individuals minimized energy expenditure during locomotion, moving downhill along an oblique route cutting the slope in order to avoid the steepest gradient. In keeping with...

Fig. S1 related to Figs. 3, 4. (A) (left) PSD modulation for the minimum contrast level (K = 0) of experimental (black) and simulated data for WT (orange, left) and FHM1 (green, right). $${\chi}_r^2=0.34$$ χ r 2 = 0.34 for WT and $${\chi}_r^2=0.28$$ χ r 2 = 0.28 for FHM1. (B) Cortical narrow band power modulation as a function of thalamic narrow band strength in the FHM1 model. The model was set with (red) and...

Supplemental material, sj-docx-4-tai-10.1177_20499361221141771 for The impact of Antimicrobial Stewardship Programmes in paediatric emergency department and primary care: a systematic review by Giulia Brigadoi, Sara Rossin, Davide Visentin, Elisa Barbieri, Carlo Giaquinto, Liviana Da Dalt and Daniele Donà in Therapeutic Advances in Infectious Disease

Supplemental material, sj-docx-5-tai-10.1177_20499361221141771 for The impact of Antimicrobial Stewardship Programmes in paediatric emergency department and primary care: a systematic review by Giulia Brigadoi, Sara Rossin, Davide Visentin, Elisa Barbieri, Carlo Giaquinto, Liviana Da Dalt and Daniele Donà in Therapeutic Advances in Infectious Disease

Additional file 8: Supplementary Table 2. Molecular validation of RFL_Contig3228_2154)associated with SCSb with HRM and rhAMP assays.

Additional file 8: Supplementary Table 2. Molecular validation of RFL_Contig3228_2154)associated with SCSb with HRM and rhAMP assays.

Networks of Unmanned Aerial Vehicles (UAVs) are emerging in many application domains, e.g., military surveillance. To perform collaborative tasks, the involved UAVs exchange several types of information, e.g., sensor data and commands. The major question here is how to schedule the tasks under dynamic traffic flows to provide network services. Existing solutions use the Round-Robin Strategy (RRS), where the tasks are scheduled statistically by dividing the time into fixed-length rounds. However, the RRS wastes significant...

Additional file 1. Classification of treatment center countries of JIA patients into geographic regions.

Additional file 2. Classification of reported autoimmune diseases in parents of included JIA patients.