Additional file 3: Figure S3. LAMC2 expression correlates with CSC content and function. (a) qPCR analysis of LAMC2 gene expression in adherent cells versus spheres. Data are normalized to GAPDH and are presented as fold change in gene expression relative to adherent cells (indicated as Adh). (b) Western blot analysis of LAMC2 in adherent cells versus spheres. Parallel β-ACTIN immunoblotting was performed. (c) qPCR analysis for CD44, CD133 and L1CAM genes in adherent cells versus...

Additional file 4: Figure S4. Knockdown of LAMC2 does not affect cell growth. (a) Western blot analysis of LAMC2 in sh empty and LAMC2 knockdown cells. Parallel β-ACTIN immunoblotting was performed. (b) qPCR analysis for LAMC2 and CD44 gene expression in sh empty and LAMC2 knockdown cells. Data are normalized to GAPDH and are presented as fold change in gene expression relative to sh empty. (c) Representative images of sh empty and LAMC2 knockdown cells...

Additional file 6: Figure S6. Knockdown of LAMC2 affects tumorigenicity. (a) Representative images from a gelatin degradation assay of sh empty and LAMC2 knockdown cells. Nuclei were stained with Hoechst 33342 (blue), green represents actin (Alexa Fluor™ 488 Phalloidin) and red illustrates gelatin (Rodhamine). The white dashed line circles indicate the areas of degradation. (b) Degradation potential of sh empty and LAMC2 knockdown cells. (c) qPCR analysis for EMT genes in sh empty and LAMC2...

Additional file 7: Figure S7. Generation of LAMC2-EGFP knock-in human PDAC cells. (a) Flow cytometry for IRFP in PDAC cells 48 hours post-nucleofection. All cytometry gates were established based on isotype controls. (b) Flow cytometry for EGFP in PDAC cells 20 days post-nucleofection. All cytometry gates were established based on isotype controls. (c) PCR gDNA specific integration analysis. The positions of primers are indicated by arrows. (d) FACS profiles showing the expression of EGFP in...

Additional file 5: Figure S5. Loss of LAMC2 reduces stemness. (a) Flow cytometry for apoptotic cells as determined by AnnexinV/PI staining in control and LAMC2 knockdown cells. (b) Flow cytometry quantification of CD44 in sh empty and LAMC2 knockdown cells. (c) qPCR analysis of CD133 in sh empty and LAMC2 knockdown cells. Data are normalized to GAPDH and are presented as fold change in gene expression relative to sh empty. (d) Flow cytometry analysis of...

Additional file 9: Figure S9. Global gene expression profiles of LAMC2-EGFP+ and EGFP--derived tumors. Heat map of differentially expressed genes in EGFP+ and EGFP- cells isolated from tumors.

Additional file 2: Figure S2. Increased LAMC2 expression is associated with aggressive signature. (a) Dimensional reduction plot (DimPlot) of multiple cell types identified in PDAC primary tumors by single-cell RNA sequencing (scRNA-Seq). The clusters are color-coded based on cell types identified using known cell type-specific markers and are visualized using t-Distributed Stochastic Neighbor embedding (t-SNE). (b) Violin plot showing expression levels of LAMC2 in stromal versus ductal (tumor) cells identified in PDAC primary tumors by...

Additional file 1: Figure S1. Increased LAMC2 expression is associated with an unfavorable outcome in PDAC. (a) Representative images of IHC staining for LAMC2 (brown) in tissue sections from pancreatic islets. (b) H-score for LAMC2 expression. (c) Number of PDAC tumors (Human Protein Atlas database) classified based on LAMC2 IHC staining, intensity and quantity. (d) Patients distribution according to gender, age and TNM. (e) H-score and grade distribution according to gender and age. (f) Kaplan-Meier...

Additional file 1: Figure S1. Increased LAMC2 expression is associated with an unfavorable outcome in PDAC. (a) Representative images of IHC staining for LAMC2 (brown) in tissue sections from pancreatic islets. (b) H-score for LAMC2 expression. (c) Number of PDAC tumors (Human Protein Atlas database) classified based on LAMC2 IHC staining, intensity and quantity. (d) Patients distribution according to gender, age and TNM. (e) H-score and grade distribution according to gender and age. (f) Kaplan-Meier...

Cancers require telomere maintenance mechanisms for unlimited replicative potential. They achieve this through TERT activation or alternative telomere lengthening associated with ATRX or DAXX loss. Here, as part of the ICGC/TCGA Pan-Cancer Analysis of Whole Genomes (PCAWG) Consortium, we dissect whole-genome sequencing data of over 2500 matched tumor-control samples from 36 different tumor types aggregated within the ICGC/TCGA Pan-Cancer Analysis of Whole Genomes (PCAWG) Consortium to characterize the genomic footprints of these mechanisms. While the...

Endoplasmic reticulum stress is an emerging significant player in the molecular pathology of connective tissue disorders. In response to endoplasmic reticulum stress, cells can upregulate macroautophagy/autophagy, a fundamental cellular homeostatic process used by cells to degrade and recycle proteins or remove damaged organelles. In these scenarios, autophagy activation can support cell survival. Here we demonstrated by in vitro and in vivo approaches that megakaryocytes derived from col6a1−⁄− (collagen, type VI, alpha 1) null mice display...

Supplemental material, sj-docx-1-tej-10.1177_20417314231151826 for Preclinical and clinical orthotopic transplantation of decellularized/engineered tracheal scaffolds: A systematic literature review by Elena Stocco, Silvia Barbon, Marco Mammana, Giovanni Zambello, Martina Contran, Pier Paolo Parnigotto, Veronica Macchi, Maria Teresa Conconi, Federico Rea, Raffaele De Caro and Andrea Porzionato in Journal of Tissue Engineering

glass brain images using uncorrected voxel-level analysis of regions

To characterize COVID-19 epidemiology, numerous population-based studies have been undertaken to model the risk of SARS-CoV-2 infection. Less is known about what may drive the probability to undergo testing. Understanding how much testing is driven by contextual or individual conditions is important to delineate the role of individual behavior and to shape public health interventions and resource allocation. In the Val Venosta/Vinschgau district (South Tyrol, Italy), we conducted a population-representative longitudinal study on 697 individuals...

To characterize COVID-19 epidemiology, numerous population-based studies have been undertaken to model the risk of SARS-CoV-2 infection. Less is known about what may drive the probability to undergo testing. Understanding how much testing is driven by contextual or individual conditions is important to delineate the role of individual behavior and to shape public health interventions and resource allocation. In the Val Venosta/Vinschgau district (South Tyrol, Italy), we conducted a population-representative longitudinal study on 697 individuals...

Appendix 1

Supplemental Table

Initiation of protein-primed (-) strand DNA synthesis in hepatitis B virus (HBV) requires interaction of the viral polymerase with a cis-acting regulatory signal, designated epsilon (ε), located at the 5′-end of its pre-genomic RNA (pgRNA). Binding of polymerase to ε is also necessary for pgRNA encapsidation. While the mechanistic basis of this interaction remains elusive, mutagenesis studies suggest its internal 6-nt “priming loop” provides an important structural contribution. ε might therefore be considered a promising...

The details and in-depth descriptions provided in this careful report give a complete description of what is considered the oldest human pathway up to now know in the world. The so-called “P1 Pathway” has the same direction of the ledge overlooking the slope on which the so-called "Ciampate del diavolo" are preserved and some fossil footprints have been recognized also inside its space. People and animals walked along this path during prehistoric times. In the...

This supplementary paper provides the complete dimensional dataset of the fossil human footprints well known as “Ciampate del diavolo” or “Devil’s Trails”, which are preserved on the Brown Leucitic Tuff of the Roccamonfina volcano (Central-Southern Italy). All these data have been recorded through direct surveys and a careful analysis of 3D models created from high-detailed photogrammetry. We think that this rich dataset will be useful for each morphometric analysis of human fossil footprints.

This supplementary paper provides the complete visual atlas of what is considered the oldest fossil human pathway known so far in the world (~350 ka). This structure, called “P1 Pathway” is located inside the “Devil’s Trails” (“Ciampate del diavolo”) palaeontological site (Tora e Piccilli, Roccamonfina volcano, Central-Southern Italy). High detailed planimetric maps (1 mm steps), depth maps, oblique views, photographic and light controlled images created from photogrammetric 3D-models give a complete view of each part...

Introduzione critica all'elamita lineare, risorse elettroniche e bibliografia.

Grazing behaviour influences animal productivity and the conservation of grassland ecosystem services. We used GPS tracking and remote sensing (NDVI index) to monitor the grazing patterns of lactating cows on the ‘Malga Ombretta’ summer farm (1,957 m asl) in the Dolomites, eastern Italian Alps, from 5th July to 5th August 2018. The pasture area (35 ha) was grazed by a mixed herd of Simmental and Alpine grey cows (stocking density = 0.6 LU/ha) under traditional...