Additional file 2: Fig. S1. Single cell lipid yield and fatty acid profile of S.podzolica zwy-2-3 cultivated in rapamycin treatment and different C/N ratio mediums. a Single cell lipid yield stained by Nile red. b Fatty acid profiles analyzed by GC-MS. Fig. S2. Results of SpGAT1 mutant identification. a Results of amplifying hygromycin gene, homologous arm, and part of SpGAT1 gene (lane 1: marker 5000 bp, lane 2: amplifying hygromycin gene and LB homologous arm...

Additional file 20. Analysis of variance (ANOVA) results for the estimated proportions of the “Neurons” cell type in the genes differentially expressed in the medial preoptic area (MPOA), nucleus accumbens (NAc), and lateral septum (LS).)

Additional file 27. Functional enrichment of pathways from the Kyoto Encyclopedia of Genes and Genomes (KEGG) in gene co-expression modules derived from the weighted gene coexpression network analysis (WGCNA).

Additional file 1: Demographic, breast cancer epidemiological and screening factors of the female populations in China.

Additional file 3: Age-specific incidence, mortality and survival rates of breast cancer.

Supplemental Material for Construction of a Carcinoembryonic Antigen Surface-Enhanced Raman Spectroscopy (SERS) Aptamer Sensor Based on the Silver Nanorod Array Chip by Rui Li, Lijun Li, Yan Zhang, Xin Lin, Heyuanxi Guo, Chubing Lin, and Jun Feng in Applied Spectroscopy

About how to get the criterion to judge the maximum limitation of the dispersed fringe

About how to get the criterion to judge the maximum limitation of the dispersed fringe

Supplemental material, sj-docx-4-tct-10.1177_15330338221142400 for Combined Radiomics–Clinical Model to Predict Radiotherapy Response in Inoperable Stage III and IV Non-Small-Cell Lung Cancer by Wenrui Chen, Li Wang, Yu Hou, Lan Li, Li Chang, Yunfen Li, Kun Xie, Linbo Qiu, Dan Mao, Wenhui Li and Yaoxiong Xia in Technology in Cancer Research & Treatment

Additional file 21: Fig. S21. Protein expression changes in PANC-1 cell lines after HOGA1 knocked down and over expressed, related to Fig. 8. A The schematic work flow of validation experiments for the protein expression changes after HOGA1 knocked down and over expressed. B The volcano plot showing the proteins that significantly altered between PANC-1 cells with HOGA1 KD (left)/HOGA1 OE (right) and WT. The GO processes enriched by proteins upregulated in HOGA1 KD cell...

One new 6a,11a-dehydropterocarpan derivative, 6-O-methyl-anhydrotuberosin (1), one new 6a-hydroxypterocarpan, (6aR,11aR,11bR)-hydroxytuberosone (7), and seven known compounds including two 6a,11a-dehydropterocarpans (2 and 4), two coumestans (3 and 5), one isoflavonoid (6) and two other phenolic compounds (8 and 9) were isolated from the roots of Pueraria lobata. The structures of the isolated compounds were elucidated with spectroscopic and spectrometric methods (1 D and 2DNMR, HRESIMS). Compounds 1, 2, 4–5 showed potent LSD1 inhibitory activities with IC50 values...

The resistance of Staphylococcus aureus (S. aureus) to various antibiotics has increased dramatically due to the misuse of antibiotics, and thus the development of new anti-infective drugs with new targets is urgently needed to combat resistance. Caseinolytic peptidase P is a case in hydrolase that regulates the virulence level of S. aureus. Here, we found that nepetin, a small-molecule compound from traditional Chinese herbal flavonoids, effectively inhibits ClpP activity. Nepetin suppressed the virulence of S....

When used in clinical trial adaptive designs, conditional power (CP) is most commonly calculated assuming the current trend, but this approach has been criticized for substantially deviating from the actual CP due to “double-dipping” the interim data (i.e., using the interim data as part of the final analysis test statistic and as the assumption for the remainder of the trial). By contrast, CP assuming the design assumption preserves the shape of the CP curve by...

Osteoarthritis (OA) is a well-known chronic degenerative joint disease, with multiple changes in the phenotype of chondrocytes. Circular RNAs (circRNAs) have been shown to be involved in various human diseases, including OA. The purpose of this study was to determine the role of circ_0020093 in OA pathological changes in vitro. C28/I2 cells were treated with interleukin-1 beta (IL-1β) to mimic OA pathological conditions. The expression levels of circ_0020093, miR-181a-5p and ETS-related gene (ERG) mRNA were...

Supplemental Document

Additional file 2. Fig. S1: Pictures of plant strains or leaves collected in the present study.

Additional file 4: Supplementary Table 1. Composition and nutrient levels of basal diet.

A novel and efficient iodine-promoted bimolecular annulation of 2-benzylidene 1,3-indandiones has been developed. Following this protocol, a series of spirocyclopropane derivatives were furnished in good to excellent yields. The present protocol features mild conditions, short reaction time, high efficiency, good chemoselectivity and remarkable functional group tolerance.

Additional file 1: Table S1. Correlation between age, CD4+ T cells counts and score of questionnaires. Table S2. Sensitivity analysis restricted to 10 and 8 years on ART for the correlation analysis.

Additional file 1: Methods. Table S1. Participant characteristics in the GNHS. Table S2. Participant characteristics of the CHNS. Table S3. Associations between α-diversity and cognitive impairment in the GNHS (n = 1430). Table S4. Associations between α-diversity and cognitive decline in the CHNS (n = 1300). Table S5. Weights for genus features contributing to the LASSO models. Table S6. Associations between bacterial taxonomy and cognitive performance in the CHNS (n = 1300). Table S7. Associations...

Additional file 1. Supplemental methods, tables, and figures.

Additional file 6: Fig. S6. Proteomic features in tumor tissues compared with NATs of the PDAC in CPTAC cohort, related to Fig. 3. A Venn diagram summary of the number of up-regulated (left) and down-regulated (right) proteins between Fudan cohort and CPTAC cohort. B Differentially expressed proteins in tumors and NATs in CPTAC cohort (right panel). The left panel shows KEGG pathway analysis of identified differentially expressed proteins. Red, up-regulated pathways; blue, down-regulated pathways. C...

Additional file 9: Fig. S9. 8p11.22 amplification is associated with PDAC metastasis, related to Fig. 5. A Western blot assays were conducted for detecting ADAM9 protein in PANC-1 cells after si-ADAM9-1 and si-ADMA9-2 knocked down. B The scatter plot depicting the correlation between ADAM9 expression and expression of ADAM9-interacting proteins (Spearman’s correlation). C The scatter plot depicting the correlation between the abundance of SRC and pathways (GSVA score). D The boxplots indicating the protein expression...

Additional file 14: Fig. S14. Proteomic subtypes of CPTAC PDAC patients based on Fudan’s proteomic stratification, related to Fig. 6. A Consensus matrices of identified clusters (k = 2 to 5) of proteomic subtypes in CPTAC cohort based on Fudan’s proteomic stratification. B, C The consensus CDF (B) and delta area (change in CDF area) plots (C) are shown. D The heatmap depicting the relative abundance of signature proteins among three proteomic subtypes. E The...

Additional file 18: Fig. S18. Characterization of ECM subtypes in PDAC, related to Fig. 7. A H&E-stained slides of samples of deserted, inter, reactive ECM subtypes. B The expression heatmap describing differentially expressed proteins and phosphoproteins among three ECM subtypes. The enriched biological pathways were annotated on the right. C Boxplot illustrating the purity of sample among ECM subtypes. D Boxplots showing the expression of ECM markers among ECM subtypes. E IHC profiling of ECM...