Additional file 2.

Additional file 1. Figure S1. APE1 involves in DSBs formation at early phase following IR stress. (a–b) HeLa or SiHa NC and shAPE1 cells were mock-treated or treated with dose-depended IR as indicated in figures and allowed to recover for 1 h. Whole cell lysates were obtained and immunoblotting was performed to assess the γ-H2AX. H2AX and tubulin or actin was used as a loading control. (c) Representative images of Figure 1H-I were shown. Figure...

Additional file 2: Table S2. All PCR primers used in this research.

Additional file 1: Table S1. Sequences of proteasomal β5 subunits used for phylogenetic and alignment analysis. Table S2. Oligonucleotide primers (5’-3’) used in the present study.

Additional file 3. Fig S3. Normalised peak intensity of potential metabolites which influence fish muscle quality (A) L-cysteine. (B) L-lysine. (C) L-threonine. (D) glutamine. (E) 5’-IMP. (F) L-glutamate. Significance levels with *, **, and *** represent FDR < 0.05, 0.01, and 0.001 between groups, respectively (Kruskal-Wallis test).

Additional file 2: Fig. S1. Sample collection, single-cell clustering and marker gene visualization for major lineages. Fig. S2. Immunohistochemistry (IHC) staining of immune cells. Fig. S3. Cluster marker genes, tumor cell identification, cellular fractions and hematoxylin eosin (H&E) staining for epithelial cells. Fig. S4. Upregulated genes and signatures for malignant cells among TN, MPR and NMPR patients and serum estradiol dynamics. Fig. S5. Marker genes, differentially expressed genes and RNA velocity for T/NK subsets. Fig....

Additional file 4. Fig S4. Potential microbes synthesising amino acids. The curves represent the correlation between metabolites and microbes, with greener colours representing stronger positive correlations and yellower colours representing stronger negative correlations.

Additional file 1: Table S1. Demographic data for the enrolled cohorts. Table S2. In-silico pathogenicity predictions for rare variants in SPTLC1 and SPTLC2. Table S3. Enrichment analysis of rare variants in SPTLC1 and SPTLC2 in amyotrophic lateral sclerosis.

Supplementary Material 1

Additional file 12: Figure S6. Functional enrichment analysis of DSG-specific, DEG-specific and overlapping DEGs&DSGs in NY-17.

Additional file 5. Review history.

Additional file 4. Uncropped western blot images. Related to Fig. S4h in Additional file 1.

Additional file 4: Table S3. List of primers used for q-PCR analysis.

Additional file 3: Table S2. The sequences of siRNA for the knockdown analysis.

Additional file 1: Table S1. Ingredients and nutrients composition of the Control diet.

Additional file 1: Table S1. All siRNAs/shRNAs used in this research.

Additional file 1: Supplementary Table 1. Amino acid sequences of selectedSnRK2s inthe phylogenetic tree.

Additional file 3: Supplementary Table 3. Primers and oligos used in this study.

Additional file 1.

Additional file 4: Figure S4. Construction of nomogram using TCGA-STAD cohort. A A nomogram was constructed based on risk score and related clinical characteristics. B ROC curves and AUC for 1-, 3-, and 5-year OS of the nomogram. C Calibration curves of 1-, 3-, and 5-year OS in the nomogram and ideal model. D DCA results of risk score and clinical characteristics in the TCGA cohort.

Additional file 1: Fig. S1. Normalized LINP1 expression levels in a variety of tumors analyzed in TCGA database. The original data is from The Cancer Genome Atlas Program (TCGA) database and the diagram shows the expression levels of LINP1 in different types of cancer analyzed by Gene Expression Profiling Interactive Analysis (GEPIA, http://gepia.cancer-pku.cn/ ). Fig. S2. LINP1 promotes cell proliferation, migration and invasiveness in HSC-1 cells. (A) LINP1 expression was detected after LINP1 depletion in...

Additional file 7: Table S4. The influence of IKZF1 mutation on AML with different CSF3R-mutated status.

Additional file 5. Review history.

Additional file 4. Uncropped western blot images. Related to Fig. S4h in Additional file 1.

Additional file 1: Table S1. Sequences of primers used for the quantitative real-time PCR analysis1.