The current coronavirus disease 2019 (COVID-19) pandemic was the result of the rapid transmission of a highly pathogenic coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), for which there is no efficacious vaccine or therapeutic. Toward the development of a vaccine, here we expressed and evaluated as potential candidates four versions of the spike (S) protein using an insect cell expression system: receptor binding domain (RBD), S1 subunit, the wild-type S ectodomain (S-WT), and the...

The current coronavirus disease 2019 (COVID-19) pandemic was the result of the rapid transmission of a highly pathogenic coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), for which there is no efficacious vaccine or therapeutic. Toward the development of a vaccine, here we expressed and evaluated as potential candidates four versions of the spike (S) protein using an insect cell expression system: receptor binding domain (RBD), S1 subunit, the wild-type S ectodomain (S-WT), and the...

Additional file 1. Completed Standard Protocol Items: Recommendation for Interventional Trials (SPIRIT) 2013 Checklist: items addressed in this clinical trial protocol.

Despite extensive studies on drivers for public sector’s green public procurement (GPP) practice, existing studies have not yet introduced configuration analysis based on holistic perspective into this field. This study, based on the Chinese background, focuses on the public sector and develops a conceptual model from a holistic perspective. Survey data are collected and we adopt the fsQCA approach for further testing, aiming to explore which combinations of GPP motivators can induce high-level GPP practice...

It was found that bisphenol F (BPF) could be anaerobically transformed to 4,4-dihydroxybenzophenone using nitrate as an electron acceptor by Pseudomonas sp. LS. However, BPF removal was a slow process under anaerobic conditions. In the present study, effects of graphene oxide (GO) and graphene on the anaerobic transformation of BPF were studied in detail. Results showed that GO (2–10 mg/L) and graphene (2–20 mg/L) could increase the anaerobic biotransformation rate of BPF. For GO-mediated system,...

Macroautophagy/autophagy is a conserved cellular process associated with tumorigenesis and aggressiveness, while mechanisms regulating expression of autophagic machinery genes in cancers still remain elusive. Herein, we identified E2F4 (E2F transcription factor 4) as a novel transcriptional activator of cytoprotective autophagy crucial for zinc homeostasis in cancer cells. Gain- and loss-of-function studies showed that E2F4 promoted autophagy in a cell cycle-dependent manner, resulting in facilitated degradation of MT (metallothionein) proteins, elevated distribution of Zn2+ within autophagosomes,...

Macroautophagy/autophagy is a conserved cellular process associated with tumorigenesis and aggressiveness, while mechanisms regulating expression of autophagic machinery genes in cancers still remain elusive. Herein, we identified E2F4 (E2F transcription factor 4) as a novel transcriptional activator of cytoprotective autophagy crucial for zinc homeostasis in cancer cells. Gain- and loss-of-function studies showed that E2F4 promoted autophagy in a cell cycle-dependent manner, resulting in facilitated degradation of MT (metallothionein) proteins, elevated distribution of Zn2+ within autophagosomes,...

Nitric oxide (NO) is involved not only in the regulation of plant growth, development, and stress responses but also in the regulation of plant-microbe interactions. Here, we demonstrate that Piriformospora indica can induce tobacco nitrate reductase to produce a NO signal in roots which enhances nitrogen uptake capacity by inducing the expression of ammonium and nitrate transporter genes and the development of lateral root and root hair, thereby promoting tobacco growth. In addition, the NO...

Persulfate-based advanced oxidation processes (P-AOPs), as the commonly used technologies, attract intensive attention, because persulfate is stable and safe with various activation methods, and the generated reactive oxygen species (ROS) are highly active. Density functional theory (DFT) calculations can analyze the intrinsic mechanism and specific pathways of P-AOPs at microscopic level, which is difficult to be realized in experiments. Recent DFT discoveries for P-AOPs have induced tremendous interest in persulfate activation or pollutant degradation pathways,...

Persulfate-based advanced oxidation processes (P-AOPs), as the commonly used technologies, attract intensive attention, because persulfate is stable and safe with various activation methods, and the generated reactive oxygen species (ROS) are highly active. Density functional theory (DFT) calculations can analyze the intrinsic mechanism and specific pathways of P-AOPs at microscopic level, which is difficult to be realized in experiments. Recent DFT discoveries for P-AOPs have induced tremendous interest in persulfate activation or pollutant degradation pathways,...

Although cardiac disorder-related adverse events (AEs) have been reported in patients treated with aryl hydrocarbon receptor (AHR) agonists, their safety profiles remain unknown. Here, we identified significant cardiac disorders associated with AHR agonists and further evaluated their relevance. Database queries were performed using OpenVigil 2.1 and AEs voluntarily submitted to Food and Drug Administration Adverse Event Reporting System (FAERS) between 2004 and 2020 were included. This study based on the Medical Dictionary for Regulatory Activities...

Model averaging has a rich history dating from its use for combining forecasts from time-series models (Bates and Granger) and presents a compelling alternative to model selection methods. We propose a frequentist model averaging procedure defined over categorical regression splines (Ma, Racine, and Yang) that allows for mixed-data predictors, as well as nonnested and heteroscedastic candidate models. We demonstrate the asymptotic optimality of the proposed model averaging estimator, and develop a post-averaging inference theory for...

Additional file 2: Figure S1. Demarcation of patients using a SNN modularity optimization-based clustering algorithm. A Unsupervised clustering of NSCLC samples with four clusters. B Unsupervised clustering of LUAD and LUSC samples. C Distribution of LUAD and LUSC samples in four different clusters. Figure S2. Associations between NSCLC subtypes and TME features. A Levels of stromal score, immune score, ESTIMATE score, and tumor purity in LUAD (n = 512), LUSC (n = 430), and LASC...

Supplemental material, sj-pdf-1-imr-10.1177_03000605221128092 for Clinical analysis of 78 pulmonary sarcomatoid carcinomas with surgical treatment by Ting Gong, Bin Jia, Chen Chen, Zhenfa Zhang, Changli Wang in Journal of International Medical Research

Additional file 3: Age-specific incidence, mortality and survival rates of breast cancer.

Additional file 1: Supplemental Figure.

Supplemental Material for Ischemic stroke and intracranial hemorrhage in extracorporeal membrane oxygenation for COVID-19: A systematic review and meta-analysis by Yu Jin, Yang Zhang, and Jinping Liu in Perfusion

Supplementary Material 1

Additional file 2: Table S1. Table of abbreviations in the TCGA database. Table S2. Summary of sample size of public datasets. Table S3. Detailed information of immunotherapy-related gene signatures. Table S4. Detailed information of BC patients receiving Paclitaxel-dependent neoadjuvant chemotherapy. Table S5. GO and KEGG pathway enrichment analyses of ACE2 in BC. Table S6. Association between ACE2 expression and clinic-pathological features in BC.

Additional file 1: eAppendix 1. Information of participating clinics. eAppendix 2. Details of APOE genotyping. eAppendix 3. MRI parameters and review. Table S1. Sex-specific characteristics of the four groups

Supplemental Material for Garmultin-A Incites Apoptosis in CB3 Cells Through miR-17-5p by Attenuating Poly (ADP-Ribose) Polymerase-1 by Jianfei Qiu, Li Chen, Jue Yang, Krishnapriya M. Varier, Babu Gajendran, Yao Yao, Wuling Liu, Jingrui Song, Qing Rao, Qun Long, Chunmao Yuan, Xiaojiang Hao, and Yanmei Li in Dose-Response

Additional file 1. Systematic Reviews and Meta-Analyses (PRISMA) Checklist.

Additional file 5. The Code Sheet of Integration of Medical Services.

Additional file 6. The Code Sheet of Stakeholders’ Acceptability.

We identified relapsing fever (RF) Borrelia in 1.45% (145/10426) of the ticks and 1.40% (40/2850) of the wild mammals in a field investigation in China. Three RF Borrelia species, including human-pathogenic Borrelia miyamotoi, Borrelia persica and unclassified Babesia sp. were determined. Main species determined from ticks was B. miyamotoi (44.14%), followed by the unclassified Borrelia sp. (42.76%), and Borrelia theileri (13.10%). In wild mammals, main species found was B. persica (57.50%), followed by the unclassified...