1,167 Works

Pathogenicity factors of Phytophthora melonis revealed by comparative proteomics

Aqeel Ahmad, Waheed Akram, Rui Wang, Iqra Shahzadi, Muhammad Umer, Nasim Ahmad Yasin & Tingquan Wu
Deciphering the transcriptional activities of pathogenicity genes is critical to understand pathogenesis mechanisms of Phytophthora melonis, a devastating pathogen of cucurbits. In this study, we have performed comparative proteomics among P. melonis mutants to evaluate the interacting pathogenicity proteins. Structural characterization of all detected proteins discovered the physicochemical factors behind their pathogenic behavior. Results revealed that gamma radiations have an extreme potential to mutate P. melonis spores, and can be a safe alternative of chemical...

Primary graft failure following allogeneic hematopoietic stem cell transplantation: risk factors, treatment and outcomes

Juan Chen, Aiming Pang, Yuanqi Zhao, Li Liu, Runzhi Ma, Jialin Wei, Xin Chen, Yi He, Donglin Yang, Rongli Zhang, Weihua Zhai, Qiaoling Ma, Erlie Jiang, Mingzhe Han, Jiaxi Zhou & Sizhou Feng
Graft failure (GF) is an intractable complication of transplantation, which can severely affect the efficacy of the graft; however, the characteristics, incidence, and risk factors of primary GF have not been well described. This study aimed to analyze the risk factors and outcomes of primary GF to swiftly identify high-risk patients for GF. We performed a case-control study with a case-control ratio of 1:4 with 869 patients who underwent allogeneic hematopoietic stem cell transplantation (allo-HSCT)...

AFAP1 antisense RNA 1 promotes retinoblastoma progression by sponging microRNA miR-545-3p that targets G protein subunit beta 1

Wenting Tang, Li Zhang, Jing Li & Yu Guan
The oncogenic role of actin filament-associated protein 1 antisense RNA 1 (AFAP1-AS1) has been reported in retinoblastoma (RB). However, the underlying regulatory mechanisms remain poorly understood. In this study, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and Western blotting were performed to analyze the expression of AFAP1-AS1, microRNA miR-545-3p, or G protein subunit beta 1 (GNB1). Cell Counting Kit-8 (CCK-8) and Transwell migration assays were used to detect cell proliferation and migration. In addition, caspase-3 activity...

Classification and expression analysis of cucumber (Cucumis sativus L.) callose synthase (CalS) family genes and localization of CsCalS4, a protein involved in pollen development

Hongyun Wang, Shoujun Cao, Tao Li, Lili Zhang, Jiangang Yao, Xiubo Xia & Ruiqing Zhang
Callose plays an important role in plant development and response to multiple biotic and abiotic stresses. Callose synthase (CalS; EC 2.4.1.34), the main enzyme in callose synthesis, has been studied in a number of crops but not in cucumber (Cucumis sativus L.). In this study, 10 genes encoding putative callose synthase were identified and classified into four clades. Expression levels of the 10 CsCalS genes, which varied considerably, were detected in most analyzed organs. Transcription...

A transcriptomic analysis of neuropathic pain in the anterior cingulate cortex after nerve injury

Yu Zhang, Shiwei Jiang, Fei Liao, Zhifeng Huang, Xin Yang, Yu Zou, Xin He, Qulian Guo & Changsheng Huang
The anterior cingulate cortex (ACC) is a core brain region processing pain emotion. In this study, we performed RNA sequencing analysis to reveal transcriptomic profiles of the ACC in a rat chronic constriction injury (CCI) model. A total of 1628 differentially expressed genes (DEGs) were identified by comparing sham-operated rats with rats of 12 hours, 1, 3, 7, and 14 days after surgery, respectively. Although these inflammatory-related DEGs were generally increased after CCI, different kinetics...

Kavalactones isolated from Alpinia zerumbet (Pers.) Burtt. et Smith with protective effects against human umbilical vein endothelial cell damage induced by high glucose

Hualin You, Min He, Di Pan, Guanqin Fang, Yan Chen, Xu Zhang, Xiangchun Shen & Nenling Zhang
A new kavalactone, 4'-hydroxyl dihydro-5, 6-dehydrokavain (1) was isolated from the petroleum ether partition of leaves of Alpinia zerumbet (Pers.) Burtt. et Smith, together with four known kavalactone dimers, rel-1,trans-3-bis-(4-methoxy-2-oxopyran-6-yl)-cis-2,trans-4-diphenyl cyclobutene (2), aniba dimer A (3), aniba dimer C (4), 6,6'-(3,4-diphenylcyclobutane-1,2-diyl)bis(4-methoxy-2H-pyran-2-one (5). The structure of compound 1 was characterized by its MS, 1D-NMR, and 2D-NMR data, and the structures of the known compounds were determined by comparison of their spectroscopic data with those reported by...

Implementation of novel boolean logic gates for IMPLICATION and XOR functions using riboregulators

Chaoxin Chen, Qi Wu, Qingying Ke, Ting Wang, Yifan Zhang, Feiwen Wei, Xiaolong Wang & Guanglei Liu
To date, several different types of synthetic genetic switches, including riboregulators, riboswitches, and toehold switches, have been developed to construct AND, OR, NOT, NAND, NOR, and NOT IMPLICATION (NIMP) gates. The logic gate can integrate multiple input signals following a set of algorithms and generate a response only if strictly defined conditions are met. However, there are still some logic gates that have not been implemented but are necessary to build complex genetic circuits. Here,...

TET2 regulates osteoclastogenesis by modulating autophagy in OVX-induced bone loss

Chen Yang, Huaqiang Tao, Haifeng Zhang, Yu Xia, Jiaxiang Bai, Gaoran Ge, Wenming Li, Wei Zhang, Long Xiao, Yaozeng Xu, Zhirong Wang, Ye Gu, Huilin Yang, Yu Liu & Dechun Geng
Increased bone resorption by osteoclasts after estrogen deficiency is the main cause of postmenopausal osteoporosis. TET2 (tet methylcytosine dioxygenase 2) is a DNA demethylase that regulates cellular function and differentiation potential. Macroautophagy/autophagy maintains cellular homeostasis by recycling unnecessary and damaged organelles. This study revealed that TET2 promoted bone loss in oophorectomized (OVX) mice and that TET2 promoted osteoclast differentiation by regulating autophagy. Tet2 knockdown inhibited autophagy and osteoclast differentiation in vitro. Mechanistically, Tet2 knockdown increased...

The response surface optimization of β-mannanase produced by Weissella cibaria F1 and its potential in juice clarification

Hairui Ji, Huiying Cao, Li Zhao, Ruiying Na, Wenxiang Ping, Jingping Ge & Dan Zhao
A β-mannanase-producing lactic acid bacteria (LAB) was identified as Weissella cibaria F1 according to physiological and biochemical properties, morphological observations, partial sequence of 16S rRNA gene and API 50 CHL test. In order to improve the yield of β-mannanase, the response surface methodology (RSM) was originally used to optimize the fermentation conditions. The optimization results showed that when the konjac powder, glucose, and initial pH were 9.46 g/L, 14.47 g/L and 5.67, respectively, the β-mannanase...

Long intergenic non-protein coding RNA 1273 confers sorafenib resistance in hepatocellular carcinoma via regulation of methyltransferase 3

Huifang Kong, Jie Sun, Wei Zhang, Huixin Zhang & Hong Li
Hepatocellular carcinoma (HCC) is often diagnosed in patients with advanced disease who are ineligible for curative surgical therapies. Sorafenib is a first-line agent approved for the treatment of advanced HCC. However, the frequent resistance of HCC cells to sorafenib greatly reduces its efficacy. Herein, we describe a novel long non-coding RNA (lncRNA) conferring sorafenib resistance. Long intergenic non-protein coding RNA 1273 (LINC01273) was significantly overexpressed in human HCC and sorafenib-resistant tissues, linking it to poor...

Synthesis, herbicidal activity study, and molecular docking of novel acylthiourea derivatives

Binbin Jiang, Yunlong Chai, Xu He, Yan Wang, Bo Chen, Yang Li & Ranhong Li
A series of new acylthiourea derivatives were synthesized and characterized. The results of preliminary herbicidal activity tests showed that most compounds had good herbicidal activity against Digitaria adscendens and Amaranthus retroflexus, especially for compounds 4b (N-(2-chloro-4-methylsulfonylbenzoyl)-N’-(2-chlorophenyl) thiourea) and 4f (N-(2-chloro-4-methylsulfonylbenzoyl)-N’-(2,3-dichlorophenyl) thiourea). In vivo acetylhydroxylate synthase (AHAS) enzyme activity inhibition tests showed that all compounds had inhibitory activities against the enzyme at a concentration of 100 mg·L−1, with the highest inhibition rate up to 37.05% (compound...

E74 Like ETS Transcription Factor 3 is a Negative Regulator of Pathogenic Lamina Propria T Helper 17.1 Cells in Murine Colitis

Bo Hu, Hao Wang & Fei Xiao
Interleukin-17A (IL-17A)-expressing T cells, including T helper 17 (Th17) and T helper 17.1 (Th17.1) cells, play a significant role in inflammatory bowel diseases (IBDs). Identifying the mechanisms underlying the heterogeneity and plasticity of IL-17A-expressing T cells is crucial for understanding and controlling their pathogenicity. The role of E74 like ETS transcription factor 3 (ELF3) in regulating the pathogenicity of IL-17A-expressing T cells has not been studied before. Dextran sulfate sodium was used to induce acute...

Modulation of Atg genes expression in aged rat liver, brain, and kidney by caloric restriction analyzed via single-nucleus/cell RNA sequencing

Chuanbin Yang, Siyu Xia, Wei Zhang, Han-Ming Shen & Jigang Wang
Dysregulation of macroautophagy/autophagy has been closely implicated in aging. Caloric restriction (CR) is an effective intervention of aging partially via activation of autophagy. Recently, a high-throughput single-cell RNA-seq technique has been employed to detect the comprehensive transcriptomes of individual cells. However, the transcriptional networks of ATG (autophagy related) genes in the aging process and the modulation of ATG genes expression by CR at the single-cell level have not been elucidated. Here, by performing data analysis...

Discovery and SAR analysis of 5-chloro-4-((substituted phenyl)amino)pyrimidine bearing histone deacetylase inhibitors

Lin Zhang, Yiming Chen, Fahui Li, Lihui Zhang, Jinhong Feng & Lei Zhang
Histone deacetylases (HDACs) are validated targets for the development of anticancer drugs in epigenetics. In the discovery of novel HDAC inhibitors with anticancer potency, the 5-chloro-4-((substituted phenyl)amino)pyrimidine fragment is assembled as a cap group into the structure of HDAC inhibitors. The SAR revealed that presence of small groups (such as methoxy substitution) is beneficial for the HDAC inhibitory activity. In the enzyme inhibitory selectivity test, compound L20 exhibited class I selectivity with IC50 values of...

Discovery and SAR analysis of 5-chloro-4-((substituted phenyl)amino)pyrimidine bearing histone deacetylase inhibitors

Lin Zhang, Yiming Chen, Fahui Li, Lihui Zhang, Jinhong Feng & Lei Zhang
Histone deacetylases (HDACs) are validated targets for the development of anticancer drugs in epigenetics. In the discovery of novel HDAC inhibitors with anticancer potency, the 5-chloro-4-((substituted phenyl)amino)pyrimidine fragment is assembled as a cap group into the structure of HDAC inhibitors. The SAR revealed that presence of small groups (such as methoxy substitution) is beneficial for the HDAC inhibitory activity. In the enzyme inhibitory selectivity test, compound L20 exhibited class I selectivity with IC50 values of...

A plant nonenveloped double-stranded RNA virus activates and co-opts BNIP3-mediated mitophagy to promote persistent infection in its insect vector

Qifu Liang, Jiajia Wan, Huan Liu, Dongsheng Jia, Qian Chen, Aiming Wang & Taiyun Wei
Mitophagy that selectively eliminates damaged mitochondria is an essential mitochondrial quality control mechanism. Recently, mitophagy has been shown to be induced in host cells infected by a few animal viruses. Here, we report that southern rice black-streaked dwarf virus (SRBSDV), a plant nonenveloped double-stranded RNA virus, can also trigger mitophagy in its planthopper vector to prevent mitochondria-dependent apoptosis and promote persistent viral propagation. We find that the fibrillar structures constructed by the nonstructural protein P7-1...

LncRNA DLEU2 silencing impedes the migration, invasion and EMT in gastric cancer cell by suppressing PI3K/AKT signaling pathway

Jun Hu, Mingyun Wang, Yang Yang, Yajun Xing & Shuanggen Li
Context: The high expression of long non-coding RNA deleted in lymphocytic leukaemia 2 (lncRNA DLEU2) has been confirmed in gastric cancer (GC). Objective: However, the detailed mechanism concerning its involvement in GC remained unclear, which we aimed to explore in this study. Materials and methods: LncRNA DLEU2 expression in GC was estimated by bioinformatic analysis, and the relationship between the expression of DLEU2 and the clinicopathological characteristics of patients with GC was performed. qRT-PCR was...

LncRNA DLEU2 silencing impedes the migration, invasion and EMT in gastric cancer cell by suppressing PI3K/AKT signaling pathway

Jun Hu, Mingyun Wang, Yang Yang, Yajun Xing & Shuanggen Li
Context: The high expression of long non-coding RNA deleted in lymphocytic leukaemia 2 (lncRNA DLEU2) has been confirmed in gastric cancer (GC). Objective: However, the detailed mechanism concerning its involvement in GC remained unclear, which we aimed to explore in this study. Materials and methods: LncRNA DLEU2 expression in GC was estimated by bioinformatic analysis, and the relationship between the expression of DLEU2 and the clinicopathological characteristics of patients with GC was performed. qRT-PCR was...

Circ_0001955 promotes the progression of non-small cell lung cancer via miR-769-5p/EGFR axis

Li Ding, Yinan Feng & Longguang Li
To elaborate on the role of circular RNA 0001955 (circ_0001955) on the proliferation and apoptosis of non-small cell lung cancer (NSCLC) cells and its underlying mechanism. Circ_0001955 expression in NSCLC was screened out through bioinformatics analysis based on GEO database. Circ_0001955, microRNA-769-5p (miR-769-5p), and epidermal growth factor receptor (EGFR) expression in NSCLC tissues and cell lines was examined using quantitative real-time PCR (qRT-PCR) and Western blot. Cell proliferation and apoptosis were examined using the CCK-8...

Effects of selenium levels on placental oxidative stress and inflammation during pregnancy: a prospective cohort study

Jianqing Wang, Chunmei Liang, Yabin Hu, Xun Xia, Zhijuan Li, Hui Gao, Jie Sheng, Kun Huang, Sufang Wang, Peng Zhu, Jiahu Hao & Fangbiao Tao
Studies on the impact of Se levels in different pregnancy periods on placental function are limited. This cohort study sought to investigate the levels of the trace element Se and to assess their effects on placental oxidative stress (OS) and mRNA expression of inflammatory genes during pregnancy. The study population consisted of 2519 pregnant women from the Ma’anshan birth cohort. Se levels were measured in the first and second trimesters of pregnancy and in cord...

Hypoglycemic activity and mechanisms of myricetin

Junqing Qian, Jinqiu Zhang, Yan Chen, Chengen Dai, Jing Fan & Hui Guo
Myricetin has been reported to have a wide variety of beneficial physiological functions. The present study was designed to investigate the mechanism of high purity myricetin, as a hypoglycemic functional component on high fat diet (HFD) fed streptozotocin (STZ) induced diabetic rats. Four-week antihyperglycemic effects of myricetin were assayed. The results showed that continuous administration of myricetin (50 and 200 mg/kg body weight) in HFD/STZ induced diabetic rats dose-dependently reduced the body serum glucose and...

Preparation of hollow Aux-Cu2O nanospheres by galvanic replacement to enhance the selective electrocatalytic CO2 reduction to ethanol

Lijie Zhang, Ying Zhang, Hongtao Wang, Jianbing Chen & Zhongqi Cao
Electrocatalytic CO2 reduction to fuel is one of the important ways to solve energy and environmental problems. In this work, the preparation of hollow Aux-Cu2O electrocatalyst and the performance of electrocatalytic CO2 reduction to ethanol were studied. Hollow Cu2O nanospheres were prepared by a soft template method, and Aux-Cu2O composites were prepared by galvanic replacement. The characterization results of XRD and XPS reveal that Cu+ is the main chemical state of Cu in the catalysts....

Population pharmacokinetics of methotrexate in paediatric patients with acute lymphoblastic leukaemia and malignant lymphoma

Min Zhan, Yiqi Sun, Fang Zhou, Honghong Wang, Zebin Chen, Lianzhen Yan & Xingang Li
This study aimed to identify physiological and pharmacogenomic covariates and develop a population pharmacokinetic model of high-dose methotrexate (HD-MTX) in Chinese paediatric patients with acute lymphoblastic leukaemia (ALL) and malignant lymphoma.A total of 731 MTX courses and 1658 MTX plasm concentrations from 205 paediatric patients with ALL and malignant lymphoma were analysing using a non-linear mixed-effects model technique. 47 SNPs in 16 MTX-related genes were genotyped and screened as covariates. A PPK model was established...

CircMAP3K4 protects human lens epithelial cells from H2O2-induced dysfunction by targeting miR-193a-3p/PLCD3 axis in age-related cataract

Yu Ma, Yi Liu, Baotong Shu, Jianguo Yang, Liang Lv, Lixiao Zhou, Lichun Wang & Zongli Shi
Circular RNAs (circRNAs) have shown pivotal regulatory roles in multiple human ocular diseases, including age-related cataract (ARC). Here, we explored the role of circRNA mitogen-activated protein kinase kinase kinase 4 (circMAP3K4, hsa_circ_0078619) in ARC pathology and its associated mechanism. The expression of RNAs and proteins was examined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blot assay. Cell viability, senescence, proliferation, and apoptosis were analyzed by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, senescence-associated-β-galactosidase (SA-β-Gal) staining, 5-ethynyl-20-deoxyuridine...

Additional file 1 of Intravoxel incoherent motion imaging combined with diffusion kurtosis imaging to assess the response to radiotherapy in a rabbit VX2 malignant bone tumor model

Jia Guo, Weikai Sun, Cheng Dong, Zengjie Wu, Xiaoli Li, Ruizhi Zhou & Wenjian Xu
Additional file 1: Supplemental Fig. 1. Chemical and physical design of quantitative IVIM-DKI phantom. Supplemental Fig. 2. Bland–Altman plots of differences between different concentrations measurements are shown, further illustrating high similarity in IVIM-DKI parameters quantification between test and retest group. Supplemental Table 1. The coefficient of variation (CV) Values for Phantom Test-Retest Acquisitions.

Registration Year

  • 2022
    1,167

Resource Types

  • Text
    1,167

Affiliations

  • North West Agriculture and Forestry University
    1,167
  • Fudan University
    811
  • Chinese Academy of Medical Sciences & Peking Union Medical College
    804
  • Sichuan University
    765
  • Shanghai Jiao Tong University
    726
  • Zhejiang University
    712
  • Sun Yat-sen University
    677
  • Chinese Academy of Sciences
    659
  • Capital Medical University
    656
  • University of Chinese Academy of Sciences
    602