Harnessing the rhizobia-legume symbiosis is a promising strategy to confront sustainability in agriculture. Agricultural research on legume nodulation has mostly focused on abiotic parameters, yet the importance of biotic variables on legumes nodulation is still ambiguous. We explored the variation of bacterial communities in peanut rhizosphere soil under different agricultural practices (peanut fallow rotation and Vicia villosa Roth-peanut rotation) using Illumina sequencing. Samples were collected in 2020 from a 9-year study (2012–2020) located at Dongxiang...

Harnessing the rhizobia-legume symbiosis is a promising strategy to confront sustainability in agriculture. Agricultural research on legume nodulation has mostly focused on abiotic parameters, yet the importance of biotic variables on legumes nodulation is still ambiguous. We explored the variation of bacterial communities in peanut rhizosphere soil under different agricultural practices (peanut fallow rotation and Vicia villosa Roth-peanut rotation) using Illumina sequencing. Samples were collected in 2020 from a 9-year study (2012–2020) located at Dongxiang...

Three new ergosterols, colletosterols A-C (1–3), together with two known analogues 4 and 5, were isolated from the endophytic fungus Colletotrichum magnisporum associated with the leaves of Rauvolfia verticillata by a bioassay-guided fractionation method. The new structures were elucidated on the basis of extensive spectroscopic analyses and electronic circular dichroism (ECD) calculations. All the ergosterols were evaluated for their cytotoxic activities against A549 and HeLa cell lines. Compounds 1–3 exhibited notable cytotoxicity with the IC50...

Three new ergosterols, colletosterols A-C (1–3), together with two known analogues 4 and 5, were isolated from the endophytic fungus Colletotrichum magnisporum associated with the leaves of Rauvolfia verticillata by a bioassay-guided fractionation method. The new structures were elucidated on the basis of extensive spectroscopic analyses and electronic circular dichroism (ECD) calculations. All the ergosterols were evaluated for their cytotoxic activities against A549 and HeLa cell lines. Compounds 1–3 exhibited notable cytotoxicity with the IC50...

Patients with chronic obstructive pulmonary disease (COPD) suffer from persistent cough and breathlessness, which can be ameliorated by the Chinese herbal medicine glycyrrhiza. Furthermore, the SRC/MAPK pathway is activated in the process of oxidative stress and inflammation, which afflict COPD progression. Thus, this research aimed at dissecting the mechanism of compound glycyrrhiza oral solution (CGOS) relieving oxidative stress and inflammation in COPD via the SRC/MAPK pathway. After a COPD rat model was established using lipopolysaccharide...

Resistance to BCR-ABL tyrosine kinase inhibitor (TKI) is the cause of treatment failure in blast phase chronic myeloid leukaemia (BP-CML). Agents that act synergistically with BCR-ABL TKI are required to improve response. This work investigated the effects of stachydrine in CML. CML cells were treated with control or stachydrine at 20, 40 and 80 µM. Proliferation and apoptosis were examined after 72 h treatment. Combination studies were performed in four groups: control, TKI, stachydrine and...

Currently, human papillomavirus (HPV) vaccines are in short supply, so the development of HPV vaccines has a broad market prospect. The 3-, 9-, and 15-valent HPV vaccines developed by ourselves all contain HPV58-derived antigen components. It is important to detect HPV 58 during vaccine production. Here, we introduced a development process of HPV58 type-specific antibodies and a detection kit. Briefly, HPV58 L1-Virus Like Particles (VLPs) were used as antigens to immunize mice, followed by extraction...

Additional file 2: Figure S1. Original blot and images. Figure S2. HMGB2 expression is not correlated with survival of patients with LUSC.

Waning of neutralizing titres along with decline of protection efficacy after the second dose of COVID-19 vaccines was observed, including China-made inactivated vaccines. Efficacy of a heterologous boosting using one dose of a recombinant SARS-CoV-2 fusion protein vaccine (V-01) in inactivated vaccine-primed population was studied, aimed to restore the immunity. A randomized, double-blind and placebo-controlled phase III trial was conducted in healthy people aged 18 years or older in Pakistan and Malaysia. Each eligible participant...

The aim of the study was to determine molecular genetic and clinical characterization of acute myeloid leukemia (AML) with trisomy 8 as the sole chromosome abnormality, a recurrent but rare chromosomal abnormality in AML. Interphase fluorescence in situ hybridization, reverse transcriptase-quantitative polymerase chain reaction for gene rearrangement and next-generation sequencing (NGS) were performed on sole trisomy 8 AML patients. A total of 35 AML patients with trisomy 8 as the sole chromosome abnormality were screened....

The aim of the study was to determine molecular genetic and clinical characterization of acute myeloid leukemia (AML) with trisomy 8 as the sole chromosome abnormality, a recurrent but rare chromosomal abnormality in AML. Interphase fluorescence in situ hybridization, reverse transcriptase-quantitative polymerase chain reaction for gene rearrangement and next-generation sequencing (NGS) were performed on sole trisomy 8 AML patients. A total of 35 AML patients with trisomy 8 as the sole chromosome abnormality were screened....

The constituents of Ceratocarpus arenarius L., as a traditional anticancer medicine of Kazakh, were firstly profiled with UHPLC-QTOF-MS/MS. The potential compounds against EGFR-TK were virtually screened. As a result, forty-four compounds were analyzed, including 18 flavonoids, 8 steroids, 4 phenolic acids, 9 fatty acids, 1 coumarin and 4 other compounds. Among them, 9 flavonoids, N-trans-Feruloyltyramine (5), stigmasterol (11) and carthamone (38) were recognized as potential key anti-tumor constituents of C. arenarius through docking to active...

The present work aims to prove the concept of tumor-targeted drug delivery mediated by platelets. Doxorubicin (DOX) attached to nanodiamonds (ND-DOX) was investigated as the model payload drug of platelets. In vitro experiments first showed that ND-DOX could be loaded in mouse platelets in a dose-dependent manner with a markedly higher efficiency and capacity than free DOX. ND-DOX-loaded platelets (Plt@ND-DOX) maintained viability and ND-DOX could be stably held in the platelets for at least 4...

To develop and validate an ultrasonic radiomics model for predicting the recurrence and differentiation of hepatocellular carcinoma (HCC). Convolutional neural network (CNN) ResNet 18 and Pyradiomics were used to analyze gray-scale-ultrasonic images to predict the prognosis and degree of differentiation of HCC. This retrospective study enrolled 513 patients with HCC who underwent preoperative grayscale-ultrasonic imaging, and their clinical characteristics were observed. Patients were randomly divided into training (n = 413) and validation (n = 100)...

To investigate the molecular mechanism of the ADVENTITIOUS ROOTING RELATED OXYGENASE 1 (ARRO-1) gene in adventitious rooting of tree peony, the PsARRO-1 gene was isolated from Paeonia ostii. Subsequently, a yeast two-hybrid (Y2H) cDNA library using the split-ubiquitin yeast membrane system was constructed, the insert fragments lengths of pPR3-N membrane Y2H library were mainly concentrated between 500 and 3,000 bp, and the library capacity reached 1.3 × 107 CFUs, with an efficiency of recombinant of...

In this article, six new Fe3S2 cluster complexes Fe3(µ3S)2(CO)7[(Ph2P)2NR] (1-6) (R = (CH2)2CH3, CH(CH3)2, (CH2)3CH3, CH2CH(CH3)2, p-C6H4CCH and m-C6H4CCH) were successfully synthesized by reactions of Fe3S2(CO)9 and the corresponding aminodiphosphines under refluxing toluene. Meanwhile, treatment of Fe3S2(CO)9 and (Ph2P)2NCH(CH3)Ph afforded two new Fe3S2 cluster complexes Fe3(µ3-S)2(CO)7[(Ph2P)2NCH(CH3)Ph] (7) and Fe3(µ3-S)2(CO)7[(κ2-Ph2P)2NCH(CH3)(Ph)] (8). Complexes 1–8 were characterized by elemental analysis, FT-IR, NMR spectroscopy, and especially X-ray crystallography. The elctrochemical properties were investigated by cyclic voltammetry, showing that they...

In this article, six new Fe3S2 cluster complexes Fe3(µ3S)2(CO)7[(Ph2P)2NR] (1-6) (R = (CH2)2CH3, CH(CH3)2, (CH2)3CH3, CH2CH(CH3)2, p-C6H4CCH and m-C6H4CCH) were successfully synthesized by reactions of Fe3S2(CO)9 and the corresponding aminodiphosphines under refluxing toluene. Meanwhile, treatment of Fe3S2(CO)9 and (Ph2P)2NCH(CH3)Ph afforded two new Fe3S2 cluster complexes Fe3(µ3-S)2(CO)7[(Ph2P)2NCH(CH3)Ph] (7) and Fe3(µ3-S)2(CO)7[(κ2-Ph2P)2NCH(CH3)(Ph)] (8). Complexes 1–8 were characterized by elemental analysis, FT-IR, NMR spectroscopy, and especially X-ray crystallography. The elctrochemical properties were investigated by cyclic voltammetry, showing that they...

We fabricated an ultrasound activated ‘nanobomb’ as a noninvasive and targeted physical therapeutic strategy for sonodynamic therapy and priming cancer immunotherapy. This ‘nanobomb’ was rationally designed via the encapsulation of indocyanine green (ICG) and perfluoropentane (PFP) into cRGD peptide-functionalized nano-liposome. The resulting Lip-ICG-PFP-cRGD nanoparticle linked with cRGD peptide could actively targeted ID8 and TC-1 cells and elicits ROS-mediated apoptosis after triggered by low-intensity focused ultrasound (LIFU). Moreover, the phase change of PFP (from droplets to...

Additional file 1.

Additional file 1: Fig. S1. Genotyping, PGR levels fertility of AF1 mutant mice. Fig. S2. AF1_FFF mutation did not affect nuclear localization of PGR. Fig. S3. Serum concentrations of progesterone on GD3 and GD4.5 between the genotypes are not significantly different. Fig. S4. AF1 mediates progesterone suppression of estrogen regulated cell proliferation genes. Fig. S5. AF1 mediates progesterone suppression of estrogen regulated Mtorc1 signaling, interferon gamma response, and hypoxia Hallmark gene sets. Fig. S6. The...

Aeromonas caviae, an important food-borne pathogen, induces serious invasive infections and inflammation. The pro-inflammatory IL-1β functions against pathogenic infections and is elevated in various Aeromonas infection cases. However, the molecular mechanism of A. caviae-mediated IL-1β secretion remains unknown. In this study, mouse macrophages (PMs) were used to establish A. caviae infection model and multiple strategies were utilized to explore the mechanism of IL-1β secretion. IL-1β was elevated in A. caviae infected murine serum, PMs lysates...

Additional file 1: Table S1. Unadjusted Cox regression analysis investigating predictors of MACCE. Figure S1. Calculation of Gensini score of representative case.

Additional file 2: Table S2. Akaike information criterion for the prognostic signature.

Topological inverse design of fabrication-constrained nanophotonic devices via adaptive projection method: supplemental document

The structure design, performance analysis, and process optimization of CNT/MOF-derived hierarchical composite play an important role in the development of high-performance microwave absorbing materials. Herein, the preparation, morphology evolution, and electromagnetic wave absorption mechanism of CNT/MOF-derived hierarchical composite were systematically investigated. The regulation mechanism was revealed by studying the changes in the morphological characteristics, electromagnetic properties, and microwave absorbing performance of CNT/MOF-derived hierarchical composite under different process parameters. The results show that the morphological characteristics...