283 Works

Study on the roles of melatonin in regulating dermal fibroblast growth in Liaoning cashmere goats by transcriptome sequencing

Mei Jin, Xinyue Qiu, Jing'ai Piao, Lijuan Zhang, Jun Piao & Fengqin Zhao
In this study, the genes related to the Downy growth of Liaoning cashmere goats were screened for their expression with simultaneous melatonin administration, so as to investigate the effects of target genes on the proliferation of skin fibroblasts in this animal species. Genes related to the villus growth of skin fibroblasts were screened by in vitro transcriptome sequencing and verified by qPCR. In addition, gene overexpression and interference were used to study the effects of...

Cytotoxicity and polyol pathway inhibitory activities of chemical constituents isolated from the pericarp of Toona sinensis

Rongshen Wang, Di Liu, Xiaoxiao Liu, Fan Liu, Lulu Xuan, Ying Tang & Wanzhong Li
Toona sinensis is a medicinal and edible plant that belongs to the genus Toona of family Meliaceae. Phytochemical investigations carried out on this plant, seven apotirucallane-type triterpenoids (1–7), two cycloartane-type triterpenoids (8–9), four sterols (10–13), two sesquiterpenes (14–15), four phenols (16–19), and one lignin (20) were isolated from the pericarp of T. sinensis by silica gel column and preparative middle pressure liquid chromatography. Their structures were identified by interpretation of NMR and comparison with those...

Association study of hypertension susceptibility genes ITGA9, MOV10, and CACNB2 with preeclampsia in Chinese Han population

Qian Xin, Gang Xin, Li Li, Wenjuan Sun, Wen Jiang, Jue Wang, Yun Luan, Ying Zhang, Ling Cheng, Shuhong Duan, Fanzhen Hong, Qinghong Ji & Weihong Ma
Preeclampsia (PE) is a disorder that occurs during the pregnancy and could affect the maternal and perinatal mortality as well as morbidity. The aim of our study is to investigate the associations between the hypertension susceptibility genes ITGA9, MOV10 and CACNB2 with PE in Chinese Han population. A case–control study including 178 PE patients and 202 healthy controls was conducted to assess the associations between three loci (ITGA9 rs155524, MOV10 rs2932538 and CACNB2 rs4373814) and...

The feasibility of one-stage flexible ureteroscopy lithotripsy in solitary kidney patients with 1–3 cm renal stones and risk factors of renal function changes

Yang Pan, Han Chen, Hualin Chen, Xiaoxiang Jin, Yunxiao Zhu & Gang Chen
To compare perioperative outcomes and long-term renal function changes between prior stenting (PS) and not prior stenting (NPS) before flexible ureteroscopy lithotripsy (f-URS) for solitary kidney patients. Solitary kidney patients with 10–30 mm renal stones were enrolled in this historical control study. Perioperative parameters and complications were compared. Stone-free was defined as the absence of any residual stones on a CT scan. Renal function changes were evaluated by estimated glomerular filtration rate (eGFR) and adjusted...

Post-translational knockdown and post-secretional modification of EsxA determine contribution of EsxA membrane permeabilizing activity for mycobacterial intracellular survival

Yanqing Bao, Lin Wang & Jianjun Sun
Current genetic studies (e.g. gene knockout) have suggested that EsxA and EsxB function as secreted virulence factors that are essential for Mycobaterium tuberculosis (Mtb) intracellular survival, specifically in mediating phagosome rupture and translocation of Mtb to the cytosol of host cells, which further facilitates Mtb intracellular replicating and cell-to-cell spreading. The EsxA-mediated intracellular survival is presumably achieved by its pH-dependent membrane-permeabilizing activity (MPA). However, the data from other studies have generated a discrepancy regarding the...

Vagus nerve plays a pivotal role in CD4+ T cell differentiation during CVB3-induced murine acute myocarditis

Li Yue-Chun, Xiao-Hong Gu, Ge Li-Sha, De-Pu Zhou, Chao Xing, Xiao-Ling Guo, Lu-Lu Pan, Shi-Yang Song, Li-Li Yu, Guang-Yi Chen, Jia-Feng Lin & Mao-Ping Chu
Abnormalities in CD4+ T cell (Th cell) differentiation play an important role in the pathogenesis of viral myocarditis (VMC). Our previous studies demonstrated that activation of the cholinergic anti-inflammatory pathway (CAP) alleviated the inflammatory response. In addition, we observed that right cervical vagotomy aggravates VMC by inhibiting CAP. However, the vagus nerve’s effect on differentiation of CD4+ T cells has not been studied in VMC mice to date. In this study, we investigated the effects...

Microbial profiling identifies potential key drivers in gastric cancer patients

Yonghong Li, Jia Wang, Mengge Wang, Yongshun Gao, Cheng-Yun Jin, Xiaojing Shi, Boyang Ji, Yongjun Wei & Hongmin Liu
Gastric cancer (GC) is the fifth most commonly diagnosed cancer and the third leading cause of cancer-related death in the world. Microbiota is believed to be associated with GC. Growing evidences showed Helicobacter pylori played a key role in GC development. However, little was known about the microbiota in gastric juices and tissues in GC patients, and thus it was difficult to understand other potential microbial causation for GC. Here, we collected the gastric juice...

Tumor-targeted Gd-doped mesoporous Fe3O4 nanoparticles for T1/T2 MR imaging guided synergistic cancer therapy

Shaohui Zheng, Shang Jin, Min Jiao, Wenjun Wang, Xiaoyu Zhou, Jie Xu, Yong Wang, Peipei Dou, Zhen Jin, Changyu Wu, Jingjing Li, Xinting Ge & Kai Xu
In this study, a novel intelligent nanoplatform to integrate multiple imaging and therapeutic functions for targeted cancer theranostics. The nanoplatform, DOX@Gd-MFe3O4 NPs, was constructed Gd-doped mesoporous Fe3O4 nanoparticles following with the doxorubicin (DOX) loading in the mesopores of the NPs. The DOX@Gd-MFe3O4 NPs exhibited good properties in colloidal dispersity, photothermal conversion, NIR triggered drug release, and high T1/T2 relaxicity rate (r1=9.64 mM−1s−1, r2= 177.71 mM−1s−1). Benefiting from the high MR contrast, DOX@Gd-MFe3O4 NPs enabled simultaneous...

The synergistic antitumor activity of 3-(2-nitrophenyl) propionic acid-paclitaxel nanoparticles (NPPA-PTX NPs) and anti-PD-L1 antibody inducing immunogenic cell death

Xiao-Chuan Duan, Li-Yuan Peng, Xin Yao, Mei-Qi Xu, Hui Li, Shuai-Qiang Zhang, Zhuo-Yue Li, Jing-Ru Wang, Zhen-Han Feng, Guang-Xue Wang, Ai Liao, Ying Chen & Xuan Zhang
Cancer immunotherapy is a strategy that is moving to the frontier of cancer treatment in the current decade. In this study, we show evidence that 3-(2-nitrophenyl) propionic acid-paclitaxel nanoparticles (NPPA-PTX NPs), act as immunogenic cell death (ICD) inducers, stimulating an antitumor response which results in synergistic antitumor activity by combining anti-PD-L1 antibody (aPD-L1) in vivo. To investigate the antitumor immunity induced by NPPA-PTX NPs, the expression of both ICD marker calreticulin (CRT) and high mobility...

Upregulated HMGB1 levels in maternal–fetal interface of patients with unexplained recurrent spontaneous abortion from different sources

Jing Wang, Damin Zhu, Jiaqian Yin, Cong Ma, Xiaoqing Peng, Huijuan Zou, Yunxia Cao & Xiaofeng Xu
To investigate the expression and sources of high mobility group box 1 (HMGB1) protein in the maternal–fetal interface of patients with unexplained recurrent spontaneous abortion (URSA), and further to verify the role of HMGB1 in the etiology of URSA. 55 women at early pregnancy with URSA and 55 women undergoing selective termination of normal early pregnancy as control were included. The abortion tissues including villi and decidua were collected. The expression of HMGB1, CD45, CK7,...

On the Quantification of Boundary Layer Effects on Flame Temperature Measurements Using Line-of-sight Absorption Spectroscopy

Liuhao Ma, Kin-Pang Cheong, Min Yang, Chaokai Yuan & Wei Ren
We studied the boundary layer effects on flame temperature measurements using line-of-sight (LOS) absorption spectroscopy. The primary objective is to quantify the measurement uncertainty of the LOS-determined temperature, which is influenced by the thermal and species boundary layers existing in standard laminar premixed flames. The boundary layer thickness (δ), central flame temperature (Tc) and species concentration (Xc) are the major factors investigated in this work. Typical absorption lines of H2O in the wavelength range of...

Vinegar-processed Curcuma phaeocaulis promotes anti-angiogenic activity and reduces toxicity in zebrafish and rat models

Wan Liao, Yi Chen, Zongping Zhu, Jiao Chen, Tianhui Gao, Boonjai Limsila, Yenchit Techadamrongsin, Lei Wang, Jiali Yu, Chaomei Fu & Rui Li
Processing with vinegar could enhance the efficacy and reduce the toxicity of Curcuma phaeocaulis Valeton. (Zingiberaceae), a Chinese herbal medicine with anti-inflammatory and antitumor activities. This study investigated the vinegar processing effects by evaluating anti-angiogenic effect and toxicity of C. phaeocaulis through zebrafish and rat models. Zebrafish embryos (AB and FLk-GFP strain) were applied to evaluate toxicity, cardiotoxicity and anti-angiogenic activity of volatile oil, and water decoction of the raw and vinegar-processed C. phaeocaulis. Meanwhile,...

Vinegar-processed Curcuma phaeocaulis promotes anti-angiogenic activity and reduces toxicity in zebrafish and rat models

Wan Liao, Yi Chen, Zongping Zhu, Jiao Chen, Tianhui Gao, Boonjai Limsila, Yenchit Techadamrongsin, Lei Wang, Jiali Yu, Chaomei Fu & Rui Li
Processing with vinegar could enhance the efficacy and reduce the toxicity of Curcuma phaeocaulis Valeton. (Zingiberaceae), a Chinese herbal medicine with anti-inflammatory and antitumor activities. This study investigated the vinegar processing effects by evaluating anti-angiogenic effect and toxicity of C. phaeocaulis through zebrafish and rat models. Zebrafish embryos (AB and FLk-GFP strain) were applied to evaluate toxicity, cardiotoxicity and anti-angiogenic activity of volatile oil, and water decoction of the raw and vinegar-processed C. phaeocaulis. Meanwhile,...

Removal of harmful algae by Shigella sp. H3 and Alcaligenes sp. H5: algicidal pathways and characteristics

Gang Xue, Xiaonuan Wang, Chenlan Xu, Binxue Song & Hong Chen
Application of algicidal bacteria is a promising technology to control harmful algal blooms (HABs). In this study, algicidal bacteria strains Shigella sp. H3 and Alcaligenes sp. H5 were obtained via two different isolation methods from the same lake water sample, with optimal algicidal efficiencies 96% and 74% against algae mixture. The Shigella sp. H3 and Alcaligenes sp. H5 lysed algae cells through cells-to-cells direct contact and secretion of algicidal metabolites, respectively. The stronger algicidal capability...

A novel immune-related long non-coding RNA signature improves the prognosis prediction in the context of head and neck squamous cell carcinoma

Lin Chen, Zhimou Cai, Kexing Lyu, Zhiwei Cai & Wenbin Lei
The tumor immune microenvironment plays an important role in head and neck squamous cell carcinoma (HNSCC). Reliable prognostic signatures able to accurately predict the immune landscape and survival rate of HNSCC patients are crucial to ensure an individualized/effective treatment. Here, we used HNSCC transcriptomic and clinical data retrieved from The Cancer Genome Atlas and identified differentially expressed immune-related long non-coding RNAs (DEirlncRNAs). DEirlncRNA pairs were recognized using univariate analysis. Cox and Lasso regression analyses were...

Novel modified nano-silica/polymer composite in water-based drilling fluids to plug shale pores

Fei Liu, Zheng Zheng, Xiuying Wang, Xiaqing Li, Zhaoxiang Zhang, Xuewu Wang, Xiaodong Dai, Yanping Xin, Qingxue Liu, Hailei Yao, Shunyao Jiang, Chao Liu & Xingyan Li
The rapid growth of global energy demand necessitates high-performance water-based drilling fluids (WDFs) with excellent-plugging performance in the deep excavation of shale gas formation. Herein, we report an organic-inorganic nanocomposite (NS-D) as a plugging agent in WDFs to plug the nanoporous of shale and abate the hydration expansion of shale by integrating the advantages of inorganic and polymer nano plugging agents. The results of Fourier Transform infrared spectroscopy (FTIR) and proton nuclear magnetic resonance (H-NMR)...

Extraction and characterization of a functional protein from Millettia speciosa Champ. leaf

Si-Yuan Luo, Zhi Huang, Xi Chen, Min-Hua Zong & Wen-Yong Lou
Natural plant-derived protein with excellent bioactivities has attracted much attention so a functional protein with molecular weight of 15.2 kDa was extracted from Millettia speciosa Champ. leaf for the first time. Under the pH of 12.0, solid-liquid ratio of 1:40 (w/v), extraction time of 2.0 h, and extraction temperature of 50 °C, the highest extracting efficiency (79.25 ± 0.78%) of the Millettia speciosa Champ. leaf protein (MLP) was achieved. The main structure of MLP contained...

Prenylaromadendrane-type diterpenoids from the gum resin of Boswellia sacra flueck and their cytotoxic effects

Jia-Jia Wang, Xin-Yue Suo, Hao-Ran Sun, Xue Wang, Ming-Bao Lin, Jin-Hua Wang, Jian-Dong Jiang & Teng-Fei Ji
Two new prenylaromadendrane-type diterpenoids, and three known analogues, were isolated from the ethanol extract of the gum resin of B. sacra Flueck. The structures of the new compounds were elucidated using 1 D and 2 D NMR spectroscopic analyses, mass spectrometric data, circular dichroism spectra, and comparison with the other compounds in the literature. One diterpenoid represents the first example of an acetoxyl-substituted prenylaromadendranoid in frankincense. All compounds exhibited notable cytotoxicity against human malignant glioma...

Prenylaromadendrane-type diterpenoids from the gum resin of Boswellia sacra flueck and their cytotoxic effects

Jia-Jia Wang, Xin-Yue Suo, Hao-Ran Sun, Xue Wang, Ming-Bao Lin, Jin-Hua Wang, Jian-Dong Jiang & Teng-Fei Ji
Two new prenylaromadendrane-type diterpenoids, and three known analogues, were isolated from the ethanol extract of the gum resin of B. sacra Flueck. The structures of the new compounds were elucidated using 1 D and 2 D NMR spectroscopic analyses, mass spectrometric data, circular dichroism spectra, and comparison with the other compounds in the literature. One diterpenoid represents the first example of an acetoxyl-substituted prenylaromadendranoid in frankincense. All compounds exhibited notable cytotoxicity against human malignant glioma...

The safety and tolerability of alkaloids from Alstonia scholaris leaves in healthy Chinese volunteers: a single-centre, randomized, double-blind, placebo-controlled phase I clinical trial

Zhong-Ping Gou, Yun-Li Zhao, Lin-Ling Zou, Ying Wang, Shi-Qing Shu, Xiao-Hong Zhu, Li Zheng, Qi Shen, Zhu Luo, Jia Miao, Yong-Sheng Wang, Xiao-Dong Luo & Ping Feng
Capsule of alkaloids from the leaf of Alstonia scholaris (L.) R.Br. (Apocynaceae) (CALAS) is a new investigational botanical drug (No. 2011L01436) for bronchitis, post-infectious cough and asthma. To observe the clinical safety and tolerability of CALAS. Subjects were assigned to eight cohorts, and each received randomly CALAS or placebo in one of single ascending dose (SAD) of 8, 40, 120, 240, 360, 480, or in one of multiple ascending dose (MAD) of 40 or 120...

sj-pdf-4-prf-10.1177_02676591211031095 – Supplemental material for Del Nido cardioplegia for myocardial protection in adult cardiac surgery: a systematic review and update meta-analysis

Kerong Zhai, Xingdong Cheng, Pengbin Zhang, Shilin Wei, Jian Huang, Xiangyang Wu, Bingren Gao & Yongnan Li
Supplemental material, sj-pdf-4-prf-10.1177_02676591211031095 for Del Nido cardioplegia for myocardial protection in adult cardiac surgery: a systematic review and update meta-analysis by Kerong Zhai, Xingdong Cheng, Pengbin Zhang, Shilin Wei, Jian Huang, Xiangyang Wu, Bingren Gao and Yongnan Li in Perfusion

sj-pdf-4-prf-10.1177_02676591211031095 – Supplemental material for Del Nido cardioplegia for myocardial protection in adult cardiac surgery: a systematic review and update meta-analysis

Kerong Zhai, Xingdong Cheng, Pengbin Zhang, Shilin Wei, Jian Huang, Xiangyang Wu, Bingren Gao & Yongnan Li
Supplemental material, sj-pdf-4-prf-10.1177_02676591211031095 for Del Nido cardioplegia for myocardial protection in adult cardiac surgery: a systematic review and update meta-analysis by Kerong Zhai, Xingdong Cheng, Pengbin Zhang, Shilin Wei, Jian Huang, Xiangyang Wu, Bingren Gao and Yongnan Li in Perfusion

Comprehensive Analysis of Monocarboxylate Transporter 4 (MCT4) expression in breast cancer prognosis and immune infiltration via integrated bioinformatics analysis

Chen Yuan, Jie Zhang, Jianjuan Lou, Siqi Wang, Yanni Jiang, Feiyun Wu & Shouju Wang
Lactate blunts the anticancer immune response in breast cancer (BC). However, little is known about the exact effect of lactate transporters such as monocarboxylate transporter 4 (MCT4) on immunotherapy. In this study, we investigated the expression status and prognostic value of MCT4 in BC through large-scale transcriptome data. Our results showed that MCT4 was overexpressed in BC, particularly in the basal-like molecular subtype. Overexpression of MCT4 was significantly correlated with high BC lesion grade and...

Effects of percutaneous balloon mitral valvuloplasty in patients with mitral stenosis and atrial fibrillation: a systematic review and meta-analysis

Bojiang Liu, Yunyun Wang, Yingwu Liu, Peng Wu & Tong Li
The study aimed to systematically review the existing literature and assess the effects of percutaneous balloon mitral valvuloplasty (PBMV) in patients with mitral stenosis and atrial fibrillation (AF) as opposed to sinus rhythm (SR). Eligible studies were identified from six electronic databases before June 2021. The primary outcome was mitral valve area (MVA), and secondary outcomes were hemodynamic measurements, in-hospital complications, and long-term outcomes. Relative risks (RRs) or weighted mean differences (WMDs) with 95% confidence...

Additional file 2 of Using a WeChat mini-program-based lactation consultant intervention to increase the consumption of mother’s own milk by preterm infants in the neonatal intensive care unit: a study protocol for a cluster randomized controlled trial

Jie Huo, Xinping Wu, Chuanli Gu, Zhangbin Yu, Jun Zhang, Xiaohui Chen, Jingai Zhu, Feng Liu, Beibei Liu, Qianqian Li & Shuping Han
Additional file 2.

Registration Year

  • 2021
    283

Resource Types

  • Text
    283

Affiliations

  • Zhejiang University
    283
  • Chinese Academy of Medical Sciences & Peking Union Medical College
    163
  • Shanghai Jiao Tong University
    153
  • Fudan University
    150
  • Sun Yat-sen University
    143
  • Sichuan University
    130
  • Chinese Academy of Sciences
    123
  • Huazhong University of Science and Technology
    119
  • Capital Medical University
    116
  • Nanjing Medical University
    114