3,352 Works

One novel naphthalene derivative and other constituents with anti-complementary activities from the aerial parts of Dracocephalum moldavica

Jia-Ming Wang, Jin-Feng Sun, Long Jin, Meng-Jie Wang, Yan-Yan Huang, Mei Jin, Wei Zhou & Gao Li
One novel naphthalene derivative, 2-octa-2′,4′,6′-atriynenaphthalene (1), together with eighteen known compounds (2–19) were isolated from the aerial parts of Dracocephalum moldavica L. Compounds 2, 8, 10, 13, 15–17 and 19 were obtained from the family Lamiaceae for the first time, and compounds 11 and 18 were firstly identified from the genus of Dracocephalum. All the isolates were evaluated for anti-complementary activities through the classical and alternative pathways, and the targets of the most active compounds...

Aaptamine derivatives with CDK2 inhibitory activities from the South China Sea sponge Aaptos suberitoides

Qian-Qian He, Yu-Qing Man, Kun-Lai Sun, Li-Juan Yang, Yan Wu, Jing Du, Wei-Wei Chen, Juan-Juan Dai, Na Ni, Shuang Miao & Kai-Kai Gong
Three new aaptamines (1–3) together with two known derivatives (4–5) were isolated from the South China Sea sponge Aaptos suberitoides. The structures of all compounds were unambiguously elucidated by spectroscopic analyses as well as the comparison with literature data. All the compounds were evaluated for their cytotoxic activities against five human cancer cell lines including H1299, H520, SCG7901, CNE-2 and SW680 cells. As a result, compounds 3–5 showed moderate cytotoxicities against H1299 and H520 cells...

Analysis of the genetic diversity of grain legume germplasm resources in China and the development of universal SSR primers

Runnan Zhou, Siqi Yang, Bo Zhang, Zhaoming Qi, Dawei Xin, Anyu Su, Sinan Li, Peng Cheng, Yunqi Bai, Zhengong Yin, Binshuo Zhang, Yujing Zhao, Ying Zhao, Qingshan Chen & Xiaoxia Wu
The research objects of this paper are Vigna angularis, Vigna radiata and Phaseolus vulgaris, which are important grain legume resources in China. However, the genetic structure between the three species is not clear. Therefore, this study developed a set of universal gSSR (simple sequence repeat) primers based on the whole-genome sequences of adzuki beans, mung beans and common beans and analysed the genetic diversity of the test resources based on universal gSSR loci. A total...

Analysis of the genetic diversity of grain legume germplasm resources in China and the development of universal SSR primers

Runnan Zhou, Siqi Yang, Bo Zhang, Zhaoming Qi, Dawei Xin, Anyu Su, Sinan Li, Peng Cheng, Yunqi Bai, Zhengong Yin, Binshuo Zhang, Yujing Zhao, Ying Zhao, Qingshan Chen & Xiaoxia Wu
The research objects of this paper are Vigna angularis, Vigna radiata and Phaseolus vulgaris, which are important grain legume resources in China. However, the genetic structure between the three species is not clear. Therefore, this study developed a set of universal gSSR (simple sequence repeat) primers based on the whole-genome sequences of adzuki beans, mung beans and common beans and analysed the genetic diversity of the test resources based on universal gSSR loci. A total...

Mitochondrial-bound hexokinase 1 can affect the glucolipid metabolism and reactive oxygen species production in goose fatty liver

Minmeng Zhao, Qian Wang, Long Liu, Tuoyu Geng & Daoqing Gong
To investigate the functions of hexokinase 1 (HK1) in the formation of goose fatty liver, a total of 40 healthy 63-day-old male Landes geese were selected and randomly assigned to a control group and an overfeeding treatment. In addition, the overexpression or RNA interference assay of HK1, and transcriptome analysis after HK1 overexpression were performed in the goose primary hepatocytes. Data showed that the mRNA expression of hepatic HK1 was upregulated in overfed treatment compared...

Mitochondrial-bound hexokinase 1 can affect the glucolipid metabolism and reactive oxygen species production in goose fatty liver

Minmeng Zhao, Qian Wang, Long Liu, Tuoyu Geng & Daoqing Gong
To investigate the functions of hexokinase 1 (HK1) in the formation of goose fatty liver, a total of 40 healthy 63-day-old male Landes geese were selected and randomly assigned to a control group and an overfeeding treatment. In addition, the overexpression or RNA interference assay of HK1, and transcriptome analysis after HK1 overexpression were performed in the goose primary hepatocytes. Data showed that the mRNA expression of hepatic HK1 was upregulated in overfed treatment compared...

Formation and characterization of Pt-Cu-IPMC with high specific surface area and dendritic electrode

Aifen Tian, Jiahua Li, Xixi Wang, Yue Sun, Xinrong Zhang, Shanshan Yao & Huiling Du
Ionic polymer–metal composite (IPMC) is a kind of electroactive intelligent material. The dendritically interfacial electrodes (DIEs) is very important to the performance of IPMC. Herein, the copper–platinum composite electrode (Pt-Cu-IPMC) with a high specific area DIEs was prepared by combining impregnation-reduction plating platinum (IRP-Pt), autocatalytic plating platinum (ACP-Pt) and chemical reduction plating copper (CRP-Cu). The effects of DIEs and the cycles of ACP-Pt have been systematically investigated on the electrode’s thickness, micro-morphology and electromechanical response...

Identification of novel susceptibility methylation loci for pancreatic cancer in a two-phase epigenome-wide association study

Ziqiao Wang, Yue Lu, Myriam Fornage, Li Jiao, Jianjun Shen, Donghui Li & Peng Wei
The role of DNA methylation and its interplay with gene expression in the susceptibility to pancreatic cancer (PanC) remains largely unexplored. To fill in this gap, we conducted an integrative two-phase epigenome-wide association study (EWAS) of PanC using genomic DNA from 44 cases and 556 controls (20 local controls and 536 public controls in the Framingham Heart Study) in phase 1 and 23 cases and 22 controls in phase 2. We validated the findings using...

Short divalent ethacrynic amides as pro-inhibitors of glutathione S-transferase isozyme Mu and potent sensitisers of cisplatin-resistant ovarian cancers

Bangtian Xu, Tingting Tong, Xin Wang, Fang Liu, Xiang Zhang, Xiaolei Hu, Xinpeng Li, Xiaolan Yang & Fei Liao
The linking of ethacrynic acid with ethylenediamine and 1,4-butanediamine gave EDEA and BDEA, respectively, as membrane-permeable divalent pro-inhibitors of glutathione S-transferase (GST). Their divalent glutathione conjugates showed subnanomolar inhibition and divalence-binding to GSTmu (GSTM) (PDB: 5HWL) at ∼0.35 min−1. In cisplatin-resistant SK-OV-3, COC1, SGC7901 and A549 cells, GSTM activities probed by 15 nM BDEA or EDEA revealed 5-fold and 1.0-fold increases in cisplatin-resistant SK-OV-3 and COC1 cells, respectively, in comparison with the susceptible parental cells....

Short divalent ethacrynic amides as pro-inhibitors of glutathione S-transferase isozyme Mu and potent sensitisers of cisplatin-resistant ovarian cancers

Bangtian Xu, Tingting Tong, Xin Wang, Fang Liu, Xiang Zhang, Xiaolei Hu, Xinpeng Li, Xiaolan Yang & Fei Liao
The linking of ethacrynic acid with ethylenediamine and 1,4-butanediamine gave EDEA and BDEA, respectively, as membrane-permeable divalent pro-inhibitors of glutathione S-transferase (GST). Their divalent glutathione conjugates showed subnanomolar inhibition and divalence-binding to GSTmu (GSTM) (PDB: 5HWL) at ∼0.35 min−1. In cisplatin-resistant SK-OV-3, COC1, SGC7901 and A549 cells, GSTM activities probed by 15 nM BDEA or EDEA revealed 5-fold and 1.0-fold increases in cisplatin-resistant SK-OV-3 and COC1 cells, respectively, in comparison with the susceptible parental cells....

Two new cycloartane triterpenoid glycosides from the rhizomes of Actaea vaginata

Shunqin Zeng, Xiangyuan Li, Zixuan Zhao, Yan Lin, Yindi Zhu, Guoxu Ma, Xiaohong Zhao, Xudong Xu, Meichun Wu, Haifeng Wu, Qiongyu Zou & Yongtao Li
Two new cycloartane triterpenoid glycosides, soulieoside V (1) and 15-deacetylbeesioside O (2), together with one known compound, beesioside J (3), were isolated from the ethanolic extract of the rhizomes of Actaea vaginata. Their structures were elucidated by spectroscopic methods and by comparison with data reported in the literature. All the compounds were tested for their cytotoxic activities against human cancer cell lines.

LncRNA ZEB1-AS1/miR-1224-5p / MAP4K4 axis regulates mitochondria-mediated HeLa cell apoptosis in persistent Chlamydia trachomatis infection

Fangzhen Luo, Yating Wen, Lanhua Zhao, Shengmei Su, Wenbo Lei, Lili Chen, Chaoqun Chen, Qiulin Huang & Zhongyu Li
Persistent infection of Chlamydia trachomatis is thought to be responsible for the debilitating sequelae of blinding trachoma and infertility. Inhibition of host cell apoptosis is a persistent C. trachomatis infection mechanism. ZEB1-AS1 is a long non-coding RNA (lncRNA), which was up-regulated in persistent C. trachomatis infection in our previous work. In this study, we investigated the role of ZEB1-AS1 in persistent infection and the potential mechanisms. The results showed that ZEB1-AS1 was involved in the...

Abietane diterpenoids with neuroprotective activities from Phlegmariurus carinatus

Yang Liu, Qiang Wang, Dong-Kun Zheng, De Zhang, Zhen Xie, Jia-Wei Hu, Xin-Hua Xie, Jing Li & Shu-Ping Jiang
Two new abietane diterpenoids, phlecarinatone A (1) and phlecarinatone B (2), along with two known analogues (3 and 4), were isolated from Phlegmariurus carinatus. The structures of 1 − 4 were unambiguously elucidated by comprehensive spectroscopic analyses. Abietane diterpenoids were isolated from the plant for the first time. All isolates were tested for their neuroprotective activities against H2O2-induced SH-SY5Y cells injury, and compound 2 showed moderate effect at the concentrations ranging from 5 ∼ 20...

Upstream open reading frames mediate autophagy-related protein translation

Ying Yang, Damian Gatica Mizala, Xu Liu, Runliu Wu, Rui Kang, Daolin Tang & Daniel J. Klionsky
Macroautophagy/autophagy, a highly conserved catabolic pathway that maintains proper cellular homeostasis is stringently regulated by numerous autophagy-related (Atg) proteins. Many studies have investigated autophagy regulation at the transcriptional level; however, relatively little is known about translational control. Here, we report the upstream open reading frames (uORFs)-mediated translational control of multiple Atg proteins in Saccharomyces cerevisiae and in human cells. The translation of several essential autophagy regulators in yeast, including Atg13, is suppressed by canonical uORFs...

Conyza blinii responds to the changes of exogenous iron through auxin-terpenoids metabolism pathway

Ming Yang, Tianrun Zheng, Junyi Zhan, Maojia Wang, Wenjun Sun, Min Zhou, Zizhong Tang, TongLiang Bu, Qingfeng Li & Hui Chen
As an essential trace element, iron is a necessary micronutrient for most organisms. Conyza blinii (C. blinii) is a traditional Chinese medicine grown in Sichuan, China. We chose C. blinii as material to explore the way plants respond to iron. Our results showed that iron increased the content of endogenous auxin, glandular trichomes (GTs) density and the content of blinin, the characteristic diterpene in C. blinii. Most of the key enzymes in the blinin synthesis...

Aspartate aminotransferase-to-platelet ratio index for predicting late recurrence of hepatocellular carcinoma after radiofrequency ablation

Xinyuan Zhang, Yujing Xin, Yi Yang, Yi Chen, Xiao-jing Cao, Yanan Wang, Qingsheng Fan, Xiang Zhou & Xiao Li
Our study aimed to explore the prognostic value of the aspartate aminotransferase–platelet ratio index (APRI) and to develop a new nomogram for patients with hepatocellular carcinoma (HCC) who experience late recurrence after radiofrequency ablation (RFA). To date, no study has explored the value of APRI for assessing the late recurrence of HCC after RFA. The prognostic value of APRI was evaluated and validated in our multicenter retrospective analysis. A total of 466 HCC patients undergoing...

Aspartate aminotransferase-to-platelet ratio index for predicting late recurrence of hepatocellular carcinoma after radiofrequency ablation

Xinyuan Zhang, Yujing Xin, Yi Yang, Yi Chen, Xiao-jing Cao, Yanan Wang, Qingsheng Fan, Xiang Zhou & Xiao Li
Our study aimed to explore the prognostic value of the aspartate aminotransferase–platelet ratio index (APRI) and to develop a new nomogram for patients with hepatocellular carcinoma (HCC) who experience late recurrence after radiofrequency ablation (RFA). To date, no study has explored the value of APRI for assessing the late recurrence of HCC after RFA. The prognostic value of APRI was evaluated and validated in our multicenter retrospective analysis. A total of 466 HCC patients undergoing...

Extraintestinal pathogenic Escherichia coli utilizes the surface-expressed elongation factor Tu to bind and acquire iron from holo-transferrin

Yu Sun, Xuhang Wang, Jin Li, Feng Xue, Fang Tang & Jianjun Dai
Extraintestinal pathogenic Escherichia coli (ExPEC) is a common anthropozoonotic pathogen that causes systemic infections. To establish infection, ExPEC must utilize essential nutrients including iron from the host. Transferrin is an important iron source for multiple bacteria. However, the mechanism by which ExPEC utilizes transferrin remains unclear. In this study, we found that iron-saturated holo-transferrin rather than iron-free apo-transferrin promoted the vitality of ExPEC in heat-inactivated human serum. The multifunctional protein Elongation factor Tu (EFTu) worked...

Elucidating the structure and cytochrome P450-mediated mechanism for novel metabolites of GDC-0575 in rats

Chenghong Zhang, Sungjoon Cho, José G. Napolitano, David Russell, Christine Gu, Alan Deese, Chong Han, Yuan Chen & Shuguang Ma
1. GDC-0575 is an ATP-competitive small-molecule inhibitor of ChK1 that is being developed by Genentech for the treatment of various human malignancies. 2. In a radiolabeled mass balance study of GDC-0575 in rats, two novel metabolites, named M12 (-71 Da,) and M17 (+288 Da), were detected as abundant circulating metabolites. 3. Subsequent mass spectrometry and nuclear magnetic resonance analysis showed that M12 was a cyclized metabolite of GDC-0575, whereas M17 was its heterodimer to the...

Atg1-mediated Atg11 phosphorylation is required for selective autophagy by regulating its association with receptor proteins

Weijing Yao, Yixing Li, Yingcong Chen, Yuting Chen, Yu Xie, Miaojuan Ye, Ying Zhang, Xiangjun Chen, Xiaoyong Wu, Yuyao Feng, Zhi Hong, Yigang Wang, Wei Liu & Cong Yi
Atg11 is an adaptor protein required for the induction of selective autophagy via receptor binding. However, our understanding of the molecular mechanisms by which it regulates selective autophagy remains incomplete. Here, we show that Atg11 is phosphorylated by Atg1. Rapamycin treatment or starvation conditions induced slower electrophoretic mobility of Atg11 in an Atg1 kinase activity-dependent manner. Through in vitro kinase assays combined with mutagenesis, we determined that Atg1 phosphorylates S949, S1057, and S1064 residues in...

Atg1-mediated Atg11 phosphorylation is required for selective autophagy by regulating its association with receptor proteins

Weijing Yao, Yixing Li, Yingcong Chen, Yuting Chen, Yu Xie, Miaojuan Ye, Ying Zhang, Xiangjun Chen, Xiaoyong Wu, Yuyao Feng, Zhi Hong, Yigang Wang, Wei Liu & Cong Yi
Atg11 is an adaptor protein required for the induction of selective autophagy via receptor binding. However, our understanding of the molecular mechanisms by which it regulates selective autophagy remains incomplete. Here, we show that Atg11 is phosphorylated by Atg1. Rapamycin treatment or starvation conditions induced slower electrophoretic mobility of Atg11 in an Atg1 kinase activity-dependent manner. Through in vitro kinase assays combined with mutagenesis, we determined that Atg1 phosphorylates S949, S1057, and S1064 residues in...

Transcriptomic analysis of the innate immune signatures of a SARS-CoV-2 protein subunit vaccine ZF2001 and an mRNA vaccine RRV

Qian Wang, Ziyang Song, Jinghuan Yang, Qian He, Qunying Mao, Yu Bai, Jianyang Liu, Chaoqiang An, Xujia Yan, Bopei Cui, Lifang Song, Dong Liu, Miao Xu & Zhenglun Liang
Analysis of large-scale gene expression post vaccination can provide an overview of immune responses. We used transcriptional approaches to comprehensively analyze the innate immune response signatures elicited by protein subunit (PS) vaccine ZF2001 and an mRNA vaccine named RRV. A fine-grained time-dependent dissection of large-scale gene expression post immunization revealed that ZF001 induced MHC class II-related genes, including cd74 and H2-Aa, more expeditiously than the RRV. Notably, the RRV induced MHC class I-related genes such...

Identification of HPCAL1 as a specific autophagy receptor involved in ferroptosis

Xin Chen, Xinxin Song, Jingbo Li, Ruoxi Zhang, Chunhua Yu, Zhuan Zhou, Jiao Liu, Siyan Liao, Daniel J. Klionsky, Guido Kroemer, Jinbao Liu, Daolin Tang & Rui Kang
Selective macroautophagy/autophagy maintains cellular homeostasis through the lysosomal degradation of specific cellular proteins or organelles. The pro-survival effect of selective autophagy has been well-characterized, but the mechanism by which it drives cell death is still poorly understood. Here, we use a quantitative proteomic approach to identify HPCAL1 (hippocalcin like 1) as a novel autophagy receptor for the selective degradation of CDH2 (cadherin 2) during ferroptosis. HPCAL1-dependent CDH2 depletion increases susceptibility to ferroptotic death by reducing...

Upstream open reading frames mediate autophagy-related protein translation

Ying Yang, Damián Gatica, Xu Liu, Runliu Wu, Rui Kang, Daolin Tang & Daniel J. Klionsky
Macroautophagy/autophagy, a highly conserved catabolic pathway that maintains proper cellular homeostasis is stringently regulated by numerous autophagy-related (Atg) proteins. Many studies have investigated autophagy regulation at the transcriptional level; however, relatively little is known about translational control. Here, we report the upstream open reading frame (uORF)-mediated translational control of multiple Atg proteins in Saccharomyces cerevisiae and in human cells. The translation of several essential autophagy regulators in yeast, including Atg13, is suppressed by canonical uORFs...

Aetiology of severe community acquired pneumonia in adults identified by combined detection methods: a multi-centre prospective study in China

Jieming Qu, Jing Zhang, Yu Chen, Yi Huang, Yusang Xie, Min Zhou, Yuping Li, Dongwei Shi, Jinfu Xu, Qiuyue Wang, Bei He, Ning Shen, Bin Cao, Danyang She, Yi Shi, Xin Su, Hua Zhou, Hong Fan, Feng Ye, Qiao Zhang, Xinlun Tian & Guoxiang Lai
Severe Community Acquired Pneumonia (SCAP) challenges public health globally. Considerable improvements in molecular pathogen testing emerged in the last few years. Our prospective study combinedly used traditional culture, antigen tests, PCR and mNGS in SCAP pathogen identification with clinical outcomes. From June 2018 to December 2019, we conducted a multi-centre prospective study in 17 hospitals of SCAP patients within 48 hours of emergency room stay or hospitalization in China. All clinical data were uploaded into...

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Affiliations

  • Zhejiang University
    3,352
  • Chinese Academy of Medical Sciences & Peking Union Medical College
    2,194
  • Shanghai Jiao Tong University
    2,039
  • Fudan University
    1,753
  • Sichuan University
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  • Capital Medical University
    1,593
  • Huazhong University of Science and Technology
    1,565
  • Sun Yat-sen University
    1,549
  • Chinese Academy of Sciences
    1,464
  • Nanjing Medical University
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