2,578 Works

Anti-gastric cancer effect of Salidroside through elevating miR-99a expression

Lin Yang, Yanan Yu, Qi Zhang, Xiaoyu Li, Cuiping Zhang, Tao Mao, Siliang Liu & Zibin Tian
Salidroside is an active ingredient extracted from Rhodiola rosea that has anti-tumor activities. The current paper attempted to assess the impact of Salidroside on gastric cancer (GC) and explore the potential mechanism. GC cell lines (SNU-216 and MGC803) and gastric epithelial cell line GES-1 were treated with Salidroside. CCK-8 assay, colony formation assay, flow cytometry and Transwell assay were respectively performed to evaluate GC cells phenotype. qRT-PCR and western blot were conducted to reveal the...

Enhanced cytotoxicity of a redox-sensitive hyaluronic acid-based nanomedicine toward different oncocytes via various internalization mechanisms

Yunai Du, Sheng Wang, Tianhao Zhang, Dongsheng He, Jiasheng Tu & Yan Shen
Receptor-mediated active targeting and tumor microenvironment responsive systems from polymeric micelles have been studied for rapid cellular internalization and triggered drug release. Previously we have constructed redox-responsive polymeric micelles composed of vitamin E succinate conjugated hyaluronic acid (HA-ss-TOS), which are able to actively target CD44 proteins and quickly release loaded drugs upon exposure to high levels of glutathione (GSH) in tumor cells. In the present study, we found that despite different cellular internalization mechanisms, micelles...

SPA: a peptide antagonist that acts as a cell-penetrating peptide for drug delivery

Jingjing Song, Sujie Huang, Zhengzheng Zhang, Bo Jia, Huan Xie, Ming Kai & Wei Zhang
Although cell-penetrating peptides (CPPs) has been proven to be efficient transporter for drug delivery, ideal peptide vectors for tumor therapy are still being urgently sought. Peptide antagonists have attracted substantial attention as targeting molecules because of their high tumor accumulation and antitumor activity compared with agonists. SPA, a derivative of substance P, is a potent antagonist that exhibits antitumor activity. Based on the amino acid composition of SPA, we speculate that it can translocate across...

Involvement of NF-κB signaling pathway in the regulation of PRKAA1-mediated tumorigenesis in gastric cancer

Yangmei Zhang, Xichang Zhou, Qinglin Zhang, Youwei Zhang, Xiang Wang & Long Cheng
AMP-activated alpha 1 catalytic subunit (PRKAA1) is one of the subunits of the mammalian 5′-AMP-activated protein kinase (AMPK) playing an important role in maintaining intracellular energy metabolism and associating with the risk of gastric cancer (GC). This paper aims to uncover the influences of PRKAA1 on the tumorigenesis of GC, as well as the underlying mechanisms. We found that Helicobacter pylori (H. pylori) infection markedly increased p-NF-κBp50 and NF-κBp50 expression, along with the PRKAA1 expression,...

Sequential delivery of dual drugs with nanostructured lipid carriers for improving synergistic tumor treatment effect

Man Xu, Guangmeng Li, Haoxiang Zhang, Xiaoming Chen, Yi Li, Qianming Yao & Maobin Xie
To improve synergistic anticancer efficacy and minimize the adverse effects of chemotherapeutic drugs, temozolomide (TMZ) and curcumin (CUR) co-loaded nanostructured lipid carriers (NLCs) were prepared by microemulsion in this study. And the physicochemical properties, drug release behavior, intracellular uptake efficiency, in vitro and in vivo anticancer effects of TMZ/CUR-NLCs were evaluated. TMZ/CUR-NLCs showed enhanced inhibitory effects on glioma cells compared to single drug loaded NLCs, which may be owing to that the quickly released CUR...

Hydrous icaritin nanorods with excellent stability improves the in vitro and in vivo activity against breast cancer

Yian Wang, Tiantian Huang, Haowen Li, Jingxin Fu, Hui Ao, Likang Lu, Meihua Han, Yifei Guo, Feng Yue & Xiangtao Wang
Due to their various biological activities that are beneficial to human health and antitumor effect, flavonoid compounds have attracted much attention in recent years. Hydrous icaritin (HICT) was such a flavonoid that can inhibit the growth of breast cancer and cancer stem cells. In order to overcome the insolubility problem, HICT was fabricated into nanorods (NRs) through anti-solvent precipitation in this paper using D-α tocopherol acid polyethylene glycol succinate and sodium oleate as a co-stabilizer...

Photoelectrochemical Immunosensor Based on ZnIn2S4/Bi2Se3 Nanocomposite for the Determination of Cardiac Troponin I

Wanxin Dong, Xinxin Mo, Yu Wang, Qin Lei & He Li
In this work, a novel flexible photoelectrochemical immunosensor is developed to determine cardiac troponin I (cTnI) in serum. The flower-like ZnIn2S4 nanospheres (ZIS), as signal amplification material, were successfully synthesized by a solvothermal method. The ZIS and Bi2Se3 were modified on the indium tin oxide- polyethylene terephthalate (ITO-PET) electrode, which effectively accelerates the electronic transition and improved the photocurrent conversion efficiency (signal on). However, the binding of antigen–antibody proteins blocks the electron transfer, resulting in...

ddPCR: a more accurate tool for SARS-CoV-2 detection in low viral load specimens

Tao Suo, Xinjin Liu, Jiangpeng Feng, Ming Guo, Wenjia Hu, Dong Guo, Hafiz Ullah, Yang Yang, Qiuhan Zhang, Xin Wang, Muhanmmad Sajid, Zhixiang Huang, Liping Deng, Tielong Chen, Fang Liu, Ke Xu, Yuan Liu, Qi Zhang, Yingle Liu, Yong Xiong, Guozhong Chen, Ke Lan & Yu Chen
Quantitative real time PCR (RT-PCR) is widely used as the gold standard for clinical detection of SARS-CoV-2. However, due to the low viral load specimens and the limitations of RT-PCR, significant numbers of false negative reports are inevitable, which results in failure to timely diagnose, cut off transmission, and assess discharge criteria. To improve this situation, an optimized droplet digital PCR (ddPCR) was used for detection of SARS-CoV-2, which showed that the limit of detection...

Robust neutralization assay based on SARS-CoV-2 S-protein-bearing vesicular stomatitis virus (VSV) pseudovirus and ACE2-overexpressing BHK21 cells

Hua-Long Xiong, Yang-Tao Wu, Jia-Li Cao, Ren Yang, Ying-Xia Liu, Jian Ma, Xiao-Yang Qiao, Xiang-Yang Yao, Bao-Hui Zhang, Ya-Li Zhang, Wang-Heng Hou, Yang Shi, Jing-Jing Xu, Liang Zhang, Shao-Juan Wang, Bao-Rong Fu, Ting Yang, Sheng-Xiang Ge, Jun Zhang, Quan Yuan, Bao-Ying Huang, Zhi-Yong Li, Tian-Ying Zhang & Ning-Shao Xia
The global pandemic of coronavirus disease 2019 (COVID-19) is a disaster for human society. A convenient and reliable neutralization assay is very important for the development of vaccines and novel drugs. In this study, a G protein-deficient vesicular stomatitis virus (VSVdG) bearing a truncated spike protein (S with C-terminal 18 amino acid truncation) was compared to that bearing the full-length spike protein of SARS-CoV-2 and showed much higher efficiency. A neutralization assay was established based...

Co-delivery of paclitaxel and anti-VEGF siRNA by tripeptide lipid nanoparticle to enhance the anti-tumor activity for lung cancer therapy

Chuanmin Zhang, Yinan Zhao, Enxia Zhang, Meilin Jiang, Defu Zhi, Huiying Chen, Shaohui Cui, Yuhong Zhen, Jingnan Cui & Shubiao Zhang
The combination of chemotherapeutic drug paclitaxel (PTX) and VEGF siRNA could inhibit cancer development with synergistic efficacy. However, efficient and safe delivery systems with high encapsulation efficiency of PTX and a long-time release of drugs are urgently needed. In this study, novel nanoparticles (PTX/siRNA/FALS) were constructed by using tripeptide lipid (L), sucrose laurate (S), and folate-PEG2000-DSPE (FA) to co-deliver PTX and siRNA. The cancer cell targeting nanoparticle carrier (PTX/siRNA/FALS) showed anticipated PTX encapsulation efficiency, siRNA...

Tranilast attenuates neuropathic pain during type-2 diabetes by inhibiting hypoxia-induced pro-inflammatory cytokines in Zucker diabetic fatty rat model

Wei Zhang, Jun Ma, Shan Wang, Tao Huang & Min Xia
The modulatory effect of tranilast on neuropathic pain in type-2 diabetes (T2DM) remains unclear. We monitored interleukin (IL)-1β, nuclear factor-κB (NF-κB) and tumour necrosis factor-α (TNF-α) levels during the progression of T2DM induced neuropathic pain in rats, and assessed the impact of tranilast treatment of increasing concentrations (0, 200 and 400 mg/kg/day via oral gavage in 1% NaHCO3 delivered as 100 mg/kg twice a day) on the levels of cytokine production, as well as on...

Immune restoration in HIV-1-infected patients after 12 years of antiretroviral therapy: a real-world observational study

Jiaye Liu, Lifeng Wang, Yuying Hou, Yan Zhao, Zhihui Dou, Ye Ma, Dawei Zhang, Yasong Wu, Decai Zhao, Zhongfu Liu, Fujie Zhang, Lei Jin, Ji-Yuan Zhang, Ruonan Xu, Ming Shi, Lei Huang, Zunyou Wu, Mengjie Han, George F. Gao & Fu-Sheng Wang
Using normalization of CD4 counts as the main evaluation parameter of complete immune restoration for HIV-1 patients under antiretroviral therapy (ART) might be not enough. A comprehensive evaluation system more accurately reflecting immune restoration are urgently needed. Totally, 91,805 HIV-1 patients from 17 tertiary hospitals in China during 2005–2018 were included in this study. Immune restoration and mortality were assessed. Patients initiated ART with baseline CD4 counts <50, 50–199, 200–349, 350–499, and ≥500 cells/μL, and...

Immune restoration in HIV-1-infected patients after 12 years of antiretroviral therapy: a real-world observational study

Jiaye Liu, Lifeng Wang, Yuying Hou, Yan Zhao, Zhihui Dou, Ye Ma, Dawei Zhang, Yasong Wu, Decai Zhao, Zhongfu Liu, Fujie Zhang, Lei Jin, Ji-Yuan Zhang, Ruonan Xu, Ming Shi, Lei Huang, Zunyou Wu, Mengjie Han, George F. Gao & Fu-Sheng Wang
Using normalization of CD4 counts as the main evaluation parameter of complete immune restoration for HIV-1 patients under antiretroviral therapy (ART) might be not enough. A comprehensive evaluation system more accurately reflecting immune restoration are urgently needed. Totally, 91,805 HIV-1 patients from 17 tertiary hospitals in China during 2005–2018 were included in this study. Immune restoration and mortality were assessed. Patients initiated ART with baseline CD4 counts <50, 50–199, 200–349, 350–499, and ≥500 cells/μL, and...

Prolonged shedding of severe acute respiratory syndrome coronavirus 2 in patients with COVID-19

Qian Li, Xiao-Shuang Zheng, Xu-Rui Shen, Hao-Rui Si, Xi Wang, Qi Wang, Bei Li, Wei Zhang, Yan Zhu, Ren-Di Jiang, Kai Zhao, Hui Wang, Zheng-Li Shi, Hui-Lan Zhang, Rong-Hui Du & Peng Zhou
Following acute infection, individuals COVID-19 may still shed SARS-CoV-2 RNA. However, limited information is available regarding the active shedding period or whether infectious virus is also shed. Here, we monitored the clinical characteristics and virological features of 38 patients with COVID-19 (long-term carriers) who recovered from the acute disease, but still shed viral RNA for over 3 months. The median carrying history of the long-term carriers was 92 days after the first admission, and the...

Prolonged shedding of severe acute respiratory syndrome coronavirus 2 in patients with COVID-19

Qian Li, Xiao-Shuang Zheng, Xu-Rui Shen, Hao-Rui Si, Xi Wang, Qi Wang, Bei Li, Wei Zhang, Yan Zhu, Ren-Di Jiang, Kai Zhao, Hui Wang, Zheng-Li Shi, Hui-Lan Zhang, Rong-Hui Du & Peng Zhou
Following acute infection, individuals COVID-19 may still shed SARS-CoV-2 RNA. However, limited information is available regarding the active shedding period or whether infectious virus is also shed. Here, we monitored the clinical characteristics and virological features of 38 patients with COVID-19 (long-term carriers) who recovered from the acute disease, but still shed viral RNA for over 3 months. The median carrying history of the long-term carriers was 92 days after the first admission, and the...

A polyoxyethylene sorbitan oleate modified hollow gold nanoparticle system to escape macrophage phagocytosis designed for triple combination lung cancer therapy via LDL-R mediated endocytosis

Yan Shen, Yun Xia, Ershuang Yang, Zixuan Ye, Yuan Ding, Jiasheng Tu, Yong Zhang & Pengcheng Xu
Presently, a combination of chemotherapy, radiotherapy, thermotherapy, and other treatments has become a hot topic of research for the treatment of cancer, especially lung cancer. In this study, novel hollow gold nanoparticles (HGNPs) were used as drug carriers, and in order to improve the targeting ability of HGNPs to a lung tumor site, polyoxyethylene sorbitol oleate (PSO) was chosen here as a target ligand since it can be specifically recognized by the low-density lipoprotein (LDL)...

Supplemental Material, CONSORT_a - Effectiveness of Continuous Positive Airway Pressure Versus Mandibular Advancement Device in Severe Obstructive Sleep Apnea Patients With Mandibular Retrognathia: A Prospective Clinical Trial

Haichun Lai, Wei Huang, Wei Chen & Desheng Wang
Supplemental Material, CONSORT_a for Effectiveness of Continuous Positive Airway Pressure Versus Mandibular Advancement Device in Severe Obstructive Sleep Apnea Patients With Mandibular Retrognathia: A Prospective Clinical Trial by Haichun Lai, Wei Huang, Wei Chen and Desheng Wang in Ear, Nose & Throat Journal

Viral RNA level, serum antibody responses, and transmission risk in recovered COVID-19 patients with recurrent positive SARS-CoV-2 RNA test results: a population-based observational cohort study

Chao Yang, Min Jiang, Xiaohui Wang, Xiujuan Tang, Shisong Fang, Hao Li, Le Zuo, Yixiang Jiang, Yifan Zhong, Qiongcheng Chen, Chenli Zheng, Lei Wang, Shuang Wu, Weihua Wu, Hui Liu, Jing Yuan, Xuejiao Liao, Zhen Zhang, Xiaolu Shi, Yijie Geng, Huan Zhang, Huanying Zheng, Min Wan, Linying Lu, Xiaohu Ren … & Qinghua Hu
Managing recovered COVID-19 patients with recurrent-positive SARS-CoV-2 RNA test results is challenging. We performed a population-based observational study to characterize the viral RNA level and serum antibody responses in recurrent-positive patients and evaluate their viral transmission risk. Of 479 recovered COVID-19 patients, 93 (19%) recurrent-positive patients were identified, characterized by younger age, with a median discharge-to-recurrent-positive length of 8 days. After readmission, recurrent-positive patients exhibited mild (28%) or absent (72%) symptoms, with no disease progression....

Viral RNA level, serum antibody responses, and transmission risk in recovered COVID-19 patients with recurrent positive SARS-CoV-2 RNA test results: a population-based observational cohort study

Chao Yang, Min Jiang, Xiaohui Wang, Xiujuan Tang, Shisong Fang, Hao Li, Le Zuo, Yixiang Jiang, Yifan Zhong, Qiongcheng Chen, Chenli Zheng, Lei Wang, Shuang Wu, Weihua Wu, Hui Liu, Jing Yuan, Xuejiao Liao, Zhen Zhang, Xiaolu Shi, Yijie Geng, Huan Zhang, Huanying Zheng, Min Wan, Linying Lu, Xiaohu Ren … & Qinghua Hu
Managing recovered COVID-19 patients with recurrent-positive SARS-CoV-2 RNA test results is challenging. We performed a population-based observational study to characterize the viral RNA level and serum antibody responses in recurrent-positive patients and evaluate their viral transmission risk. Of 479 recovered COVID-19 patients, 93 (19%) recurrent-positive patients were identified, characterized by younger age, with a median discharge-to-recurrent-positive length of 8 days. After readmission, recurrent-positive patients exhibited mild (28%) or absent (72%) symptoms, with no disease progression....

Association study of hypertension susceptibility genes ITGA9, MOV10, and CACNB2 with preeclampsia in Chinese Han population

Qian Xin, Gang Xin, Li Li, Wenjuan Sun, Wen Jiang, Jue Wang, Yun Luan, Ying Zhang, Ling Cheng, Shuhong Duan, Fanzhen Hong, Qinghong Ji & Weihong Ma
Preeclampsia (PE) is a disorder that occurs during the pregnancy and could affect the maternal and perinatal mortality as well as morbidity. The aim of our study is to investigate the associations between the hypertension susceptibility genes ITGA9, MOV10 and CACNB2 with PE in Chinese Han population. A case–control study including 178 PE patients and 202 healthy controls was conducted to assess the associations between three loci (ITGA9 rs155524, MOV10 rs2932538 and CACNB2 rs4373814) and...

High Child-Pugh and CRUB65 scores predict mortality of decompensated cirrhosis patients with COVID-19: A 23-center, retrospective study

Yong Xiao, Dongwen Wu, Xiao Shi, Shuzhong Liu, Xudong Hu, Chenliang Zhou, Xia Tian, Huimin Liu, Hui Long, Zhihong Li, Ji Wang, Tao Tan, Ying Xu, Bitao Chen, Ting Liu, Heng Zhang, Shihua Zheng, Shunlin Hu, Jun Song, Jie Tang, Jichun Song, Zhengwei Cheng, Weitian Xu, Yongxiang Shen, Wenhu Yu … & Mingkai Chen
Background: COVID-19 has rapidly become a major health emergency worldwide. The characteristic, outcome, and risk factor of COVID-19 in patients with decompensated cirrhosis remain unclear. Methods: Medical records were collected from 23 Chinese hospitals. Patients with decompensated cirrhosis and age- and sex-matched non-liver disease patients were enrolled with 1:4 ratio using stratified sampling. Results: There were more comorbidities with higher Chalson Complication Index (p < 0.001), higher proportion of patients having gastrointestinal bleeding, jaundice, ascites,...

High Child-Pugh and CRUB65 scores predict mortality of decompensated cirrhosis patients with COVID-19: A 23-center, retrospective study

Yong Xiao, Dongwen Wu, Xiao Shi, Shuzhong Liu, Xudong Hu, Chenliang Zhou, Xia Tian, Huimin Liu, Hui Long, Zhihong Li, Ji Wang, Tao Tan, Ying Xu, Bitao Chen, Ting Liu, Heng Zhang, Shihua Zheng, Shunlin Hu, Jun Song, Jie Tang, Jichun Song, Zhengwei Cheng, Weitian Xu, Yongxiang Shen, Wenhu Yu … & Mingkai Chen
Background: COVID-19 has rapidly become a major health emergency worldwide. The characteristic, outcome, and risk factor of COVID-19 in patients with decompensated cirrhosis remain unclear. Methods: Medical records were collected from 23 Chinese hospitals. Patients with decompensated cirrhosis and age- and sex-matched non-liver disease patients were enrolled with 1:4 ratio using stratified sampling. Results: There were more comorbidities with higher Chalson Complication Index (p < 0.001), higher proportion of patients having gastrointestinal bleeding, jaundice, ascites,...

Potent immunosuppressive and anti-inflammatory bisindole alkaloids from Melodinus fusiformis

Lu Liu, Ming-Jun Xie, Wen-Bing Zhou, Jin-Tang Wang, Jia-Yi Wang, Afsar Khan, Ya-Ping Liu, Jian-Xin Cao, Gui-Guang Cheng & Wen-Yuan Mao
Phytochemical investigation of Melodinus fusiformis led to a new aspidosperma-aspidosperma bisindole alkaloid (BIA), bis-19β-hydroxyvenalstonidine (1), together with three known BIAs (2–4). The structures were established by extensive analysis of their HRESIMS, NMR data, and comparing with the reported data. BIA 1 is an almost symmetrical structure, linked by C3–C14’ bond, while BIAs 2–4 are reported for the first time from the plant. The cytotoxic, immunosuppressive and anti-inflammatory activities of BIAs 1–4 were evaluated in vitro....

Potent immunosuppressive and anti-inflammatory bisindole alkaloids from Melodinus fusiformis

Lu Liu, Ming-Jun Xie, Wen-Bing Zhou, Jin-Tang Wang, Jia-Yi Wang, Afsar Khan, Ya-Ping Liu, Jian-Xin Cao, Gui-Guang Cheng & Wen-Yuan Mao
Phytochemical investigation of Melodinus fusiformis led to a new aspidosperma-aspidosperma bisindole alkaloid (BIA), bis-19β-hydroxyvenalstonidine (1), together with three known BIAs (2–4). The structures were established by extensive analysis of their HRESIMS, NMR data, and comparing with the reported data. BIA 1 is an almost symmetrical structure, linked by C3–C14’ bond, while BIAs 2–4 are reported for the first time from the plant. The cytotoxic, immunosuppressive and anti-inflammatory activities of BIAs 1–4 were evaluated in vitro....

Acute kidney injury in idiopathic membranous nephropathy with nephrotic syndrome

Tianxin Chen, Ying Zhou, Xinxin Chen, Bo Chen & Jingye Pan
The impact of acute kidney injury (AKI) on the progression of renal function in idiopathic membranous nephropathy (iMN) with nephrotic syndrome (NS) patients have not yet been reported, we sought to investigate the incidence, clinical features and prognosis of AKI in iMN with NS patients and determine clinical predictors for progression from AKI to advanced chronic kidney disease (CKD) stage. We analyzed clinical and pathological data of iMN with NS patients retrospectively collected from Jan...

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