In mammalian cells, cohesin acetyltransferases Esco1 and Esco2 acetylate cohesin subunit Smc3 to establish chromosome cohesion, ensuring the accurate chromosome segregation. However, we have previously documented that both Esco1 and Esco2 have unique substrates and roles in mouse oocyte meiosis I to orchestrate the meiotic progression, but whether these functions are conserved among species is still not determined. Here, we used porcine oocytes as a model to illustrate that Esco1 and Esco2 exerted conserved functions...

Since the outbreak of coronavirus disease 2019 (COVID-19) in Wuhan, China, it has rapidly spread across many other countries. While the majority of patients were considered mild, critically ill patients involving respiratory failure and multiple organ dysfunction syndrome are not uncommon, which could result death. We hypothesized that cytokine storm is associated with severe outcome. We enrolled 102 COVID-19 patients who were admitted to Renmin Hospital (Wuhan, China). All patients were classified into moderate, severe...

The establishment of a simple and effective method for determination of heavy metal ions has great significance due to the high hazard of heavy metal ions to public health and environments. Herein, we developed a facile way to synthesise a nanocomposite comprised of carbon nanotubes (CNTs) and metal-organic frameworks (MOFs), and explored its application in constructing an electrochemical (EC) sensor for detection of Cd2+ in aqueous solutions. The sensing surface was simply fabricated by casting...

Different primers/probes sets have been developed all over the world for the nucleic acid detection of SARS-CoV-2 by quantitative real time polymerase chain reaction (qRT-PCR) as a standard method. In our recent study, we explored the feasibility of droplet digital PCR (ddPCR) for clinical SARS-CoV-2 nucleic acid detection compared with qRT-PCR using the same primer/probe sets issued by Chinese Center for Disease Control and Prevention (CDC) targeting viral ORF1ab or N gene, which showed that...

Supplemental Material, CONSORT_b for Effectiveness of Continuous Positive Airway Pressure Versus Mandibular Advancement Device in Severe Obstructive Sleep Apnea Patients With Mandibular Retrognathia: A Prospective Clinical Trial by Haichun Lai, Wei Huang, Wei Chen and Desheng Wang in Ear, Nose & Throat Journal

Four new cembrane diterpenes, 1,13-di-epi-13-acetoxy launine P (1), 13-oxo-thunbergol (2), isocrassumol B (3) and 7α, 8α-sarcophine (4), together with two known launine P (5) and sarcophytonin B (6), were isolated from the extract of the South China Sea soft coral Sarcophyton trocheliophorum. The structures were elucidated by spectroscopic analysis, ECD analysis and comparison with literature data. All compounds were tested for their antimicrobial activity and antiviral activity. Compounds 1, 2 and 5 were found to...

5-Methylcytosine (m5C) is a type of RNA modification that exists in tRNAs and rRNAs and was recently found in mRNA. Although mRNA m5C modification has been reported to regulate diverse biological process, its function in adipogenesis remains unknown. Here, we demonstrated that knockdown of NOL1/NOP2/Sun domain family member 2 (NSUN2), a m5C methyltransferase, increased lipid accumulation of 3T3-L1 preadipocytes through accelerating cell cycle progression during mitotic clonal expansion (MCE) at the early stage of adipogenesis....

Supplemental material, sj-pdf-1-ajs-10.1177_03635465211030259 for Quadriceps Tendon Autograft Versus Bone–Patellar Tendon–Bone and Hamstring Tendon Autografts for Anterior Cruciate Ligament Reconstruction: A Systematic Review and Meta-analysis by Wenli Dai, Xi Leng, Jian Wang, Jin Cheng, Xiaoqing Hu and Yingfang Ao in The American Journal of Sports Medicine

It was found that bisphenol F (BPF) could be anaerobically transformed to 4,4-dihydroxybenzophenone using nitrate as an electron acceptor by Pseudomonas sp. LS. However, BPF removal was a slow process under anaerobic conditions. In the present study, effects of graphene oxide (GO) and graphene on the anaerobic transformation of BPF were studied in detail. Results showed that GO (2–10 mg/L) and graphene (2–20 mg/L) could increase the anaerobic biotransformation rate of BPF. For GO-mediated system,...

Cerebral infarction induces angiogenesis in the thalamus and influences functional recovery. The mechanisms underlying angiogenesis remain unclear. This study aimed to investigate the role of RTN4/Nogo-A in mediating macroautophagy/autophagy and angiogenesis in the thalamus following middle cerebral artery occlusion (MCAO). We assessed secondary neuronal damage, angiogenesis, vascular autophagy, RTN4 and S1PR2 signaling in the thalamus. The effects of RTN4-S1PR2 on vascular autophagy and angiogenesis were evaluated using lentiviral and pharmacological approaches. The results showed that...

Supplementary file 1. SPIRIT Checklist for Trials.

Patients with chronic obstructive pulmonary disease (COPD) suffer from persistent cough and breathlessness, which can be ameliorated by the Chinese herbal medicine glycyrrhiza. Furthermore, the SRC/MAPK pathway is activated in the process of oxidative stress and inflammation, which afflict COPD progression. Thus, this research aimed at dissecting the mechanism of compound glycyrrhiza oral solution (CGOS) relieving oxidative stress and inflammation in COPD via the SRC/MAPK pathway. After a COPD rat model was established using lipopolysaccharide...

We introduce a nonparametric graphical model for discrete node variables based on additive conditional independence. Additive conditional independence is a three-way statistical relation that shares similar properties with conditional independence by satisfying the semi-graphoid axioms. Based on this relation we build an additive graphical model for discrete variables that does not suffer from the restriction of a parametric model such as the Ising model. We develop an estimator of the new graphical model via the...

Phase 2 Oncology clinical trials are increasingly designed with multiple primary endpoints, such as progression-free survival (PFS) and overall survival (OS). While this gives a clearer picture of treatment benefit than trials with a single endpoint, it complicates the decision to begin a registration (phase 3) trial following a phase 2 trial. Methods for calculating the probability of study success (PrSS) assume the distribution of OS is well understood, but rarely consider the case of...

Additional file 1: Table S1. Clinicopathological characteristics of patients with gastric cancer in this study. Table S2. Different microorganisms in Proximal T and Distal T compared to their respective non tumor samples. Table S3. Significant differences in metabolites between Distal T and Distal N. Table S4. Significant differences in metabolites between Proximal T and Proximal N. Table S5. Metabolic pathway enrichment of differential metabolites between Distal T and Distal N. Table S6. Metabolic pathway enrichment...

Additional file 2: Figure S2. The microbial relative abundance of proximal, distal tumor tissues and matched non-tumor tissues at the genus level. Proximal T,proximal GC tumor tissues; Proximal N,proximal GC non-tumor tissues; Distal T, distal GC tumor tissues; Distal N, distal GC non-tumor tissues.

The perception of people with chronic kidney disease towards exercise and physical activity: a literature review

The driver’s instantaneous situation awareness in the process of take-over of vehicle control in automated driving has not yet been thoroughly investigated. The proposed research can provide a better understanding of the driver’s perceived characteristics and identify the most urgent information requirements of the on-site scenario when the driver’s eye sight returns from other distractors to the driving scene. We conducted an experiment in simulated automated driving to study the participants’ ability of instantaneous hazard...

In this work, an efficient synthesis of 3-hydroxy chromones was accomplished through oxidative cyclization mediated by lipase. This enzymatic process has salient features of environmental friendliness, mild reaction condition, satisfactory yield, green reusable biocatalyst, and short reaction time. The proposed method expands the application of lipase in organic synthesis.

A stereo interplanting system with foxtail millet beneath chestnut trees is an effective planting method to raise the utilization of land in chestnut orchards, increase yields and improve quality of chestnut nuts. Consequently, exploration of genes involved in shade tolerance response in foxtail millet and breeding shade-tolerant varieties have become urgent issues. In this study, RNA-seq of leaf samples from two shade-tolerant varieties and three shade-intolerant varieties of foxtail millet at the booting stage was...

Additional file 8: Figure S1. The correlation analysis of RNA-seq data.

Additional file 4: Age-specific number of females and standardized life expectancy

Additional file 1: Table S1. Basic data of the number of dead mice treated with different doses of PFD. Table S2. Basic data of the number of dead mice treated with PFD and hUC-MSCs. Figure S1. Flow cytometry detection of surface mesenchymal stem cell markers of hUC-MSCs. Figure S2. The primitive view of the differentiation of hUC-MSCs. A: hUC-MSCs differentiated into chondrogenic cells. B: hUC-MSCs differentiated into osteogenic cells. C: hUC-MSCs differentiated into adipogenic cells....

Supplemental Material for Garmultin-A Incites Apoptosis in CB3 Cells Through miR-17-5p by Attenuating Poly (ADP-Ribose) Polymerase-1 by Jianfei Qiu, Li Chen, Jue Yang, Krishnapriya M. Varier, Babu Gajendran, Yao Yao, Wuling Liu, Jingrui Song, Qing Rao, Qun Long, Chunmao Yuan, Xiaojiang Hao, and Yanmei Li in Dose-Response

Additional file 1: Figure S1. Cytotoxicity and cytokines analysis of the CD7 CAR T-cells. Figure S2. Diagrammatic sketch of the treatments and response. Figure S3. Infusion-related hepatic toxicities. Figure S4. Flow cytometry analysis of the fraction of T-cells and NK cells in the PB after infusion. Figure S5. Flow cytometry of the T-cell fractions in the PB after infusion of CART cells. Table S1. The result of SNP array (Cytoscan 750K/HD) at diagnosis. Table S2....