1,139 Works

TET2 regulates osteoclastogenesis by modulating autophagy in OVX-induced bone loss

Chen Yang, Huaqiang Tao, Haifeng Zhang, Yu Xia, Jiaxiang Bai, Gaoran Ge, Wenming Li, Wei Zhang, Long Xiao, Yaozeng Xu, Zhirong Wang, Ye Gu, Huilin Yang, Yu Liu & Dechun Geng
Increased bone resorption by osteoclasts after estrogen deficiency is the main cause of postmenopausal osteoporosis. TET2 (tet methylcytosine dioxygenase 2) is a DNA demethylase that regulates cellular function and differentiation potential. Macroautophagy/autophagy maintains cellular homeostasis by recycling unnecessary and damaged organelles. This study revealed that TET2 promoted bone loss in oophorectomized (OVX) mice and that TET2 promoted osteoclast differentiation by regulating autophagy. Tet2 knockdown inhibited autophagy and osteoclast differentiation in vitro. Mechanistically, Tet2 knockdown increased...

Long intergenic non-protein coding RNA 1273 confers sorafenib resistance in hepatocellular carcinoma via regulation of methyltransferase 3

Huifang Kong, Jie Sun, Wei Zhang, Huixin Zhang & Hong Li
Hepatocellular carcinoma (HCC) is often diagnosed in patients with advanced disease who are ineligible for curative surgical therapies. Sorafenib is a first-line agent approved for the treatment of advanced HCC. However, the frequent resistance of HCC cells to sorafenib greatly reduces its efficacy. Herein, we describe a novel long non-coding RNA (lncRNA) conferring sorafenib resistance. Long intergenic non-protein coding RNA 1273 (LINC01273) was significantly overexpressed in human HCC and sorafenib-resistant tissues, linking it to poor...

RTN4/Nogo-A-S1PR2 negatively regulates angiogenesis and secondary neural repair through enhancing vascular autophagy in the thalamus after cerebral cortical infarction

Peiyi Xiao, Jinmin Gu, Wei Xu, Xingyang Niu, Jian Zhang, Jingjing Li, Yicong Chen, Zhong Pei, Jinsheng Zeng & Shihui Xing
Cerebral infarction induces angiogenesis in the thalamus and influences functional recovery. The mechanisms underlying angiogenesis remain unclear. This study aimed to investigate the role of RTN4/Nogo-A in mediating macroautophagy/autophagy and angiogenesis in the thalamus following middle cerebral artery occlusion (MCAO). We assessed secondary neuronal damage, angiogenesis, vascular autophagy, RTN4 and S1PR2 signaling in the thalamus. The effects of RTN4-S1PR2 on vascular autophagy and angiogenesis were evaluated using lentiviral and pharmacological approaches. The results showed that...

Synthesis, herbicidal activity study, and molecular docking of novel acylthiourea derivatives

Binbin Jiang, Yunlong Chai, Xu He, Yan Wang, Bo Chen, Yang Li & Ranhong Li
A series of new acylthiourea derivatives were synthesized and characterized. The results of preliminary herbicidal activity tests showed that most compounds had good herbicidal activity against Digitaria adscendens and Amaranthus retroflexus, especially for compounds 4b (N-(2-chloro-4-methylsulfonylbenzoyl)-N’-(2-chlorophenyl) thiourea) and 4f (N-(2-chloro-4-methylsulfonylbenzoyl)-N’-(2,3-dichlorophenyl) thiourea). In vivo acetylhydroxylate synthase (AHAS) enzyme activity inhibition tests showed that all compounds had inhibitory activities against the enzyme at a concentration of 100 mg·L−1, with the highest inhibition rate up to 37.05% (compound...

Modulation of Atg genes expression in aged rat liver, brain, and kidney by caloric restriction analyzed via single-nucleus/cell RNA sequencing

Chuanbin Yang, Siyu Xia, Wei Zhang, Han-Ming Shen & Jigang Wang
Dysregulation of macroautophagy/autophagy has been closely implicated in aging. Caloric restriction (CR) is an effective intervention of aging partially via activation of autophagy. Recently, a high-throughput single-cell RNA-seq technique has been employed to detect the comprehensive transcriptomes of individual cells. However, the transcriptional networks of ATG (autophagy related) genes in the aging process and the modulation of ATG genes expression by CR at the single-cell level have not been elucidated. Here, by performing data analysis...

Robust statistical inference for longitudinal data with nonignorable dropouts

Yujing Shao, Wei Ma & Lei Wang
In this paper, we propose robust statistical inference and variable selection method for generalized linear models that accommodate the outliers, nonignorable dropouts and within-subject correlations. The purpose of our study is threefold. First, we construct the robust and bias-corrected generalized estimating equations (GEEs) by combining the Mallows-type weights, Huber's score function and inverse probability weighting approaches to against the influence of outliers and account for nonignorable dropouts. Subsequently, the generalized method of moments is utilized...

Proline metabolism regulation in Spartina alterniflora and SaP5CS2 gene positively regulates salt stress tolerance in transgenic Arabidopsis thaliana

Yang Zhao, Yingying He, Xixi Wang, Changfeng Qu & Jinlai Miao
It has been discovered that many plants accumulate proline in response to osmotic stress. However, the interaction between salinity stress and proline metabolism-related genes has not been unequivocally identified in Spartina alterniflora. In our research, we successfully cloned key genes: SaP5CS1 and SaP5CS2, SaOAT, SaProT and correlated with expression profiles including SaPDH were also investigated. As the key enzyme genes for proline metabolism, the up-regulated expression of SaP5CS2 played a leading part under salt stress....

Preparation of hollow Aux-Cu2O nanospheres by galvanic replacement to enhance the selective electrocatalytic CO2 reduction to ethanol

Lijie Zhang, Ying Zhang, Hongtao Wang, Jianbing Chen & Zhongqi Cao
Electrocatalytic CO2 reduction to fuel is one of the important ways to solve energy and environmental problems. In this work, the preparation of hollow Aux-Cu2O electrocatalyst and the performance of electrocatalytic CO2 reduction to ethanol were studied. Hollow Cu2O nanospheres were prepared by a soft template method, and Aux-Cu2O composites were prepared by galvanic replacement. The characterization results of XRD and XPS reveal that Cu+ is the main chemical state of Cu in the catalysts....

Additional file 1 of A CT-based nomogram for predicting the risk of adenocarcinomas in patients with subsolid nodule according to the 2021 WHO classification

Qilong Song, Biao Song, Xiaohu Li, Bin Wang, Yuan Li, Wu Chen, Zhaohua Wang, Xu Wang, Yongqiang Yu, Xuhong Min & Dongchun Ma
Additional file 1: Supplemental Table 1. The ICC values of quantitative parameters between three radiologists.

Additional file 2 of A CT-based nomogram for predicting the risk of adenocarcinomas in patients with subsolid nodule according to the 2021 WHO classification

Qilong Song, Biao Song, Xiaohu Li, Bin Wang, Yuan Li, Wu Chen, Zhaohua Wang, Xu Wang, Yongqiang Yu, Xuhong Min & Dongchun Ma
Additional file 2: Supplemental Table 2. The kappa coefficients of categorical variables between three radiologists.

An Additive Graphical Model for Discrete Data

Jun Tao, Bing Li & Lingzhou Xue
We introduce a nonparametric graphical model for discrete node variables based on additive conditional independence. Additive conditional independence is a three-way statistical relation that shares similar properties with conditional independence by satisfying the semi-graphoid axioms. Based on this relation we build an additive graphical model for discrete variables that does not suffer from the restriction of a parametric model such as the Ising model. We develop an estimator of the new graphical model via the...

Supplementary material.docx from Transcriptome analysis and mining of genes related to shade tolerance in foxtail millet (Setaria italica (L.) P. Beauv.)

Dan Liu, Yanjiao Cui, Zilong Zhao, Jing Zhang, Suying Li & Zhengli Liu
A stereo interplanting system with foxtail millet beneath chestnut trees is an effective planting method to raise the utilization of land in chestnut orchards, increase yields and improve quality of chestnut nuts. Consequently, exploration of genes involved in shade tolerance response in foxtail millet and breeding shade-tolerant varieties have become urgent issues. In this study, RNA-seq of leaf samples from two shade-tolerant varieties and three shade-intolerant varieties of foxtail millet at the booting stage was...

Additional file 4 of Microbiota and metabolites alterations in proximal and distal gastric cancer patients

Yan Yang, Daofeng Dai, Wen Jin, Yingying Huang, Yingzi Zhang, Yiran Chen, Wankun Wang, Wu Lin, Xiangliu Chen, Jing Zhang, Haohao Wang, Haibin Zhang & Lisong Teng
Additional file 4: Figure S3. Differential microbiota of gastric cancer tissues and non-tumor tissues. A Differential taxa from phylum to genus of Distal T and Distal N. B Differential taxa from phylum to genus of Proximal T and Proximal N.

Additional file 1 of Microbiota and metabolites alterations in proximal and distal gastric cancer patients

Yan Yang, Daofeng Dai, Wen Jin, Yingying Huang, Yingzi Zhang, Yiran Chen, Wankun Wang, Wu Lin, Xiangliu Chen, Jing Zhang, Haohao Wang, Haibin Zhang & Lisong Teng
Additional file 1: Table S1. Clinicopathological characteristics of patients with gastric cancer in this study. Table S2. Different microorganisms in Proximal T and Distal T compared to their respective non tumor samples. Table S3. Significant differences in metabolites between Distal T and Distal N. Table S4. Significant differences in metabolites between Proximal T and Proximal N. Table S5. Metabolic pathway enrichment of differential metabolites between Distal T and Distal N. Table S6. Metabolic pathway enrichment...

Supplementary document for Activity Enhancement of Photo-generated Carrier in CsPbBr3 Nanocrystals Improved by Cd Element - 6060264.pdf

Lianfei Yao, Xue Lou, Ning Sui, Wei Zhang, Hongwei Xiao, Xiaochun Chi, Hanzhuang Zhang & Yinghui Wang
Fitting methods and supplementary data

Psoriasis complicated with metabolic disorder is associated with traditional Chinese medicine syndrome types: a hospital-based retrospective case–control study

Xiaoying Sun, Huaibo Zhao, Ruiping Wang, Hongjin Li, Yong Wu, Kan Ze, Yonghua Su, Bin Li & Xin Li
To explore the distribution law of traditional Chinese medicine (TCM) syndrome types in patients with psoriasis vulgaris complicated by metabolic disorders based on the same pathogenic factors as blood-heat and blood-stasis in the pathogenesis of psoriasis and metabolic disorders and to further analyze the correlation between adiponectin and the distribution law. From 1 January 2018 to 31 December 2019, patients diagnosed with psoriasis in the inpatient or outpatient department of Dermatology Ward of Shanghai Yueyang...

Additional file 1 of Cooking or heating with solid fuels increased the all-cause mortality risk among mid-aged and elderly People in China

Yuxiang Yang, Yang Liu, Luolan Peng, Shuai Zhang, Changzheng Yuan, Wenyuan Li, Zuyun Liu & Yanan Ma
Supplementary Material 1

Additional file 1 of Cooking or heating with solid fuels increased the all-cause mortality risk among mid-aged and elderly People in China

Yuxiang Yang, Yang Liu, Luolan Peng, Shuai Zhang, Changzheng Yuan, Wenyuan Li, Zuyun Liu & Yanan Ma
Supplementary Material 1

Prevention of ulcerative colitis by Huangqin decoction: reducing the intestinal epithelial cell apoptosis rate through the IFN-γ/JAK/ETS signalling pathway

Xiaowei Mo, Kairui Tang, Lijing Deng, Xingyi Zhou, Xiaojuan Li, Yupei Zhang & Jing Wang
Ulcerative colitis (UC) is a chronic idiopathic inflammatory bowel disease that is closely related to inflammation and apoptosis. The traditional Chinese medicine compound preparation Huangqin decoction (HQD) has been widely used in the clinical treatment of UC, but the specific mechanism of its function is still inconclusive. To explore the pathogenesis of UC based on the IFN-γ/JAK/ETS signalling pathway, and to clarify the biological mechanism of HQD. Forty 8-week-old male C57BL/6 mice were randomly divided...

Additional file 1 of The efficacy and safety of acupuncture and nicotine replacement therapy for smoking cessation: study protocol for a randomized controlled trial

Qingsheng Wang, Runjing Dai, Hailiang Zhang, Xiaomei Jiang, Xiaojing Ma, Xiangrong Zhang, Shisan Bao, Dong Ren & Jingchun Fan
Supplementary file 1. SPIRIT Checklist for Trials.

Design, synthesis, biological evaluation and molecular docking studies of novel pleuromutilin derivatives containing nitrogen heterocycle and alkylamine groups

Qi Wang, Jie Liu, Zi-Dan Zhou, Ke-Xin Zhou, Fei Li, Qi-Wen Zhang, Shou-Kai Wang, Wei Wang, Zhen Jin & You-Zhi Tang
A series of pleuromutilin derivatives containing alkylamine and nitrogen heterocycle groups were designed and synthesised under mild conditions. The in vitro antibacterial activity of these semisynthetic derivatives against four strains of Staphylococcus aureus (MRSA ATCC 43300, S.aureus ATCC 29213, S.aureus AD3, and S.aureus 144) were evaluated by the broth dilution method. Compound 13 was found to have excellent antibacterial activity against MRSA (MIC = 0.0625 μg/mL). Furthermore, compound 13 was further studied by the time-killing...

Hyperthermia promotes exosome secretion by regulating Rab7b while increasing drug sensitivity in adriamycin-resistant breast cancer

Di Xu, Wen-Juan Tang, Yi-Zhi Zhu, Zhen Liu, Kai Yang, Ming-Xing Liang, Xiu Chen, Yang Wu, Jin-Hai Tang & Wei Zhang
To investigate the mechanism through which hyperthermia promotes exosome secretion and drug sensitivity in adriamycin-resistant breast cancer. We first evaluated the effect of hyperthermia on adriamycin-resistant breast cancer viability and used transmission electron microscopy, nanoparticle tracking analysis, and a bicinchoninic acid kit to validate the effect of hyperthermia on exosome secretion. The effective targeting molecules and pathways changed by hyperthermia were explored by RNA microarray and verified in vitro. The adriamycin-resistant MCF-7/ADR cells co-incubated with...

Factors influencing magnetic resonance imaging finding of endopelvic fascial edema after ultrasound-guided high-intensity focused ultrasound ablation of uterine fibroids

Yuhang Liu, Yang Liu, Fajin Lv, Yuqing Zhong, Zhibo Xiao & Furong Lv
Investigate the relationships between endopelvic fascial edema and its influencing factors after ultrasound-guided high-intensity focused ultrasound (USgHIFU) ablation of uterine fibroids. This retrospective study included 688 women with uterine fibroids treated by USgHIFU; based on post-treatment MRI, the patients were divided into two groups: endopelvic fascial edema group and nonedema group. The specific location of fascial edema of each patient was also recorded. Fascial edema and fibroid features and treatment parameters were set as the...

A. caviae infection triggers IL-1β secretion through activating NLRP3 inflammasome mediated by NF-κB signaling pathway partly in a TLR2 dependent manner

Qiankun Yang, Jianguo Zhang, Feixue Liu, Huizhen Chen, Wei Zhang, Haitao Yang, Nana He, Jingquan Dong & Panpan Zhao
Aeromonas caviae, an important food-borne pathogen, induces serious invasive infections and inflammation. The pro-inflammatory IL-1β functions against pathogenic infections and is elevated in various Aeromonas infection cases. However, the molecular mechanism of A. caviae-mediated IL-1β secretion remains unknown. In this study, mouse macrophages (PMs) were used to establish A. caviae infection model and multiple strategies were utilized to explore the mechanism of IL-1β secretion. IL-1β was elevated in A. caviae infected murine serum, PMs lysates...

Supplementary document for Ultraviolet-to-Infrared Broadband Photodetector and Imaging Application based on Perovskite Single Crystal - 6076905.pdf

Meng Xu, Xinyue Wang, Jiexin Weng, Jingling Shen, Yanbing Hou & Bo Zhang
Supplemental figures

Registration Year

  • 2022
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Affiliations

  • Fudan University Shanghai Cancer Center
    1,139
  • Fudan University
    955
  • Shanghai Jiao Tong University
    887
  • Chinese Academy of Medical Sciences & Peking Union Medical College
    861
  • Zhejiang University
    845
  • Sichuan University
    838
  • Sun Yat-sen University
    721
  • Chinese Academy of Sciences
    699
  • Peking University
    658
  • Capital Medical University
    625