3,499 Works

RACK1 affects the progress of G2/M by regulating Aurora-A

Suqin Shen, Huan Feng, Yichen Le, Jun Ni, Long Yu, Jiaxue Wu & Meirong Bai
Aurora-A is a serine/threonine kinase, which is overexpressed in multiple human cancers and plays a key role in tumorigenesis and tumor development. In this study, we found that the receptor of activated C-kinase1 (RACK1), an important regulator of biological functions, interacted with Aurora-A and co-localized with Aurora-A at centrosomes. Moreover, RACK1 induces the auto-phosphorylation of Aurora-A in vitro and in vivo. Depletion of RACK1 impaired the activation of Aurora-A in late G2 phase, then inhibited...

Anti-gastric cancer effect of Salidroside through elevating miR-99a expression

Lin Yang, Yanan Yu, Qi Zhang, Xiaoyu Li, Cuiping Zhang, Tao Mao, Siliang Liu & Zibin Tian
Salidroside is an active ingredient extracted from Rhodiola rosea that has anti-tumor activities. The current paper attempted to assess the impact of Salidroside on gastric cancer (GC) and explore the potential mechanism. GC cell lines (SNU-216 and MGC803) and gastric epithelial cell line GES-1 were treated with Salidroside. CCK-8 assay, colony formation assay, flow cytometry and Transwell assay were respectively performed to evaluate GC cells phenotype. qRT-PCR and western blot were conducted to reveal the...

SPA: a peptide antagonist that acts as a cell-penetrating peptide for drug delivery

Jingjing Song, Sujie Huang, Zhengzheng Zhang, Bo Jia, Huan Xie, Ming Kai & Wei Zhang
Although cell-penetrating peptides (CPPs) has been proven to be efficient transporter for drug delivery, ideal peptide vectors for tumor therapy are still being urgently sought. Peptide antagonists have attracted substantial attention as targeting molecules because of their high tumor accumulation and antitumor activity compared with agonists. SPA, a derivative of substance P, is a potent antagonist that exhibits antitumor activity. Based on the amino acid composition of SPA, we speculate that it can translocate across...

Enhanced cytotoxicity of a redox-sensitive hyaluronic acid-based nanomedicine toward different oncocytes via various internalization mechanisms

Yunai Du, Sheng Wang, Tianhao Zhang, Dongsheng He, Jiasheng Tu & Yan Shen
Receptor-mediated active targeting and tumor microenvironment responsive systems from polymeric micelles have been studied for rapid cellular internalization and triggered drug release. Previously we have constructed redox-responsive polymeric micelles composed of vitamin E succinate conjugated hyaluronic acid (HA-ss-TOS), which are able to actively target CD44 proteins and quickly release loaded drugs upon exposure to high levels of glutathione (GSH) in tumor cells. In the present study, we found that despite different cellular internalization mechanisms, micelles...

Involvement of NF-κB signaling pathway in the regulation of PRKAA1-mediated tumorigenesis in gastric cancer

Yangmei Zhang, Xichang Zhou, Qinglin Zhang, Youwei Zhang, Xiang Wang & Long Cheng
AMP-activated alpha 1 catalytic subunit (PRKAA1) is one of the subunits of the mammalian 5′-AMP-activated protein kinase (AMPK) playing an important role in maintaining intracellular energy metabolism and associating with the risk of gastric cancer (GC). This paper aims to uncover the influences of PRKAA1 on the tumorigenesis of GC, as well as the underlying mechanisms. We found that Helicobacter pylori (H. pylori) infection markedly increased p-NF-κBp50 and NF-κBp50 expression, along with the PRKAA1 expression,...

RNA based mNGS approach identifies a novel human coronavirus from two individual pneumonia cases in 2019 Wuhan outbreak

Liangjun Chen, Weiyong Liu, Qi Zhang, Ke Xu, Guangming Ye, Weichen Wu, Ziyong Sun, Fang Liu, Kailang Wu, Bo Zhong, Yi Mei, Wenxia Zhang, Yu Chen, Yirong Li, Mang Shi, Ke Lan & Yingle Liu
From December 2019, an outbreak of unusual pneumonia was reported in Wuhan with many cases linked to Huanan Seafood Market that sells seafood as well as live exotic animals. We investigated two patients who developed acute respiratory syndromes after independent contact history with this market. The two patients shared common clinical features including fever, cough, and multiple ground-glass opacities in the bilateral lung field with patchy infiltration. Here, we highlight the use of a low-input...

RNA based mNGS approach identifies a novel human coronavirus from two individual pneumonia cases in 2019 Wuhan outbreak

Liangjun Chen, Weiyong Liu, Qi Zhang, Ke Xu, Guangming Ye, Weichen Wu, Ziyong Sun, Fang Liu, Kailang Wu, Bo Zhong, Yi Mei, Wenxia Zhang, Yu Chen, Yirong Li, Mang Shi, Ke Lan & Yingle Liu
From December 2019, an outbreak of unusual pneumonia was reported in Wuhan with many cases linked to Huanan Seafood Market that sells seafood as well as live exotic animals. We investigated two patients who developed acute respiratory syndromes after independent contact history with this market. The two patients shared common clinical features including fever, cough, and multiple ground-glass opacities in the bilateral lung field with patchy infiltration. Here, we highlight the use of a low-input...

Fabrication of doxorubicin-gated mesoporous polydopamine nanoplatforms for multimode imaging-guided synergistic chemophotothermal therapy of tumors

Min Yang, Ningnan Zhang, Tao Zhang, Xian Yin & Jie Shen
A versatile theranostic agent that integrated with therapeutic and diagnostic functions is extremely essential for cancer theranostic. Herein, a multifunctional theranostic nanoplatform (PFP@MPDA-DOX) based on perfluoropentane (PFP) encapsulated mesoporous polydopamine (MPDA) is elaborately designed, followed by gating of drug doxorubicin (DOX) for preventing cargo leaking. The MPDA with pH-responsive biodegradation behavior was served as nanocarrier, which also endows the nanoplatform with a large cavity for PFP filling. The nanoparticles were then gated with DOX molecule...

Additional file 1 of Traditional Chinese medicine Lingguizhugan decoction treating non-alcoholic fatty liver disease with spleen-yang deficiency pattern: Study protocol for a multicenter randomized controlled trial

Jingjuan Xu, Ruirui Wang, Shengfu You, Lei Zhang, Peiyong Zheng, Guang Ji & Baocheng Liu
Additional file 1. SPIRIT 2013 checklist.

Establishing a high sensitivity detection method for SARS-CoV-2 IgM/IgG and developing a clinical application of this method

Chunyan Zhang, Lei Zhou, Hao Liu, Sibing Zhang, Yaping Tian, Junli Huo, Fei Li, Yao Zhang, Bo Wei, Dan Xu, Jinwei Hu, Jiayi Wang, Yuxuan Cheng, Wenjie Shi, Xiuli Xu, Jianping Zhou, Peipei Sang, Xudong Tan, Weiwei Wang, Minjie Zhang, Bin Wang, Yujun Zhou, Kan Zhang & Kunlun He
COVID-19 is caused by SARS-CoV-2 infection and was initially discovered in Wuhan. This outbreak quickly spread all over China and then to more than 20 other countries. SARS-CoV-2 fluorescent microsphere immunochromatographic test strips were prepared by the combination of time-resolved fluorescence immunoassay with a lateral flow assay. The analytical performance and clinical evaluation of this testing method was done and the clinical significance of the testing method was verified. The LLOD of SARS-CoV-2 antibody IgG...

Robust neutralization assay based on SARS-CoV-2 S-protein-bearing vesicular stomatitis virus (VSV) pseudovirus and ACE2-overexpressing BHK21 cells

Hua-Long Xiong, Yang-Tao Wu, Jia-Li Cao, Ren Yang, Ying-Xia Liu, Jian Ma, Xiao-Yang Qiao, Xiang-Yang Yao, Bao-Hui Zhang, Ya-Li Zhang, Wang-Heng Hou, Yang Shi, Jing-Jing Xu, Liang Zhang, Shao-Juan Wang, Bao-Rong Fu, Ting Yang, Sheng-Xiang Ge, Jun Zhang, Quan Yuan, Bao-Ying Huang, Zhi-Yong Li, Tian-Ying Zhang & Ning-Shao Xia
The global pandemic of coronavirus disease 2019 (COVID-19) is a disaster for human society. A convenient and reliable neutralization assay is very important for the development of vaccines and novel drugs. In this study, a G protein-deficient vesicular stomatitis virus (VSVdG) bearing a truncated spike protein (S with C-terminal 18 amino acid truncation) was compared to that bearing the full-length spike protein of SARS-CoV-2 and showed much higher efficiency. A neutralization assay was established based...

Protamine and BSA–dextran complex emulsion improves oral bioavailability and anti-tumor efficacy of paclitaxel

Guangrui Xu, Xiaoyan Bao & Ping Yao
Food protein and polysaccharide complex emulsions are safe carriers of hydrophobic drugs and nutrients. To improve oral bioavailability and therapeutic/healthy efficacy of hydrophobic drugs and nutrients, herein, protamine (PRO), a cationic cell-penetrating peptide, was introduced into protein and polysaccharide complex emulsion. The electrostatic complex of PRO and BSA–dextran conjugate (BD) produced by Maillard reaction was used as emulsifier to produce oil-in-water emulsion (@BD/PRO). The BSA molecules were crosslinked at the oil–water interface by a heat...

Protamine and BSA–dextran complex emulsion improves oral bioavailability and anti-tumor efficacy of paclitaxel

Guangrui Xu, Xiaoyan Bao & Ping Yao
Food protein and polysaccharide complex emulsions are safe carriers of hydrophobic drugs and nutrients. To improve oral bioavailability and therapeutic/healthy efficacy of hydrophobic drugs and nutrients, herein, protamine (PRO), a cationic cell-penetrating peptide, was introduced into protein and polysaccharide complex emulsion. The electrostatic complex of PRO and BSA–dextran conjugate (BD) produced by Maillard reaction was used as emulsifier to produce oil-in-water emulsion (@BD/PRO). The BSA molecules were crosslinked at the oil–water interface by a heat...

ddPCR: a more accurate tool for SARS-CoV-2 detection in low viral load specimens

Tao Suo, Xinjin Liu, Jiangpeng Feng, Ming Guo, Wenjia Hu, Dong Guo, Hafiz Ullah, Yang Yang, Qiuhan Zhang, Xin Wang, Muhanmmad Sajid, Zhixiang Huang, Liping Deng, Tielong Chen, Fang Liu, Ke Xu, Yuan Liu, Qi Zhang, Yingle Liu, Yong Xiong, Guozhong Chen, Ke Lan & Yu Chen
Quantitative real time PCR (RT-PCR) is widely used as the gold standard for clinical detection of SARS-CoV-2. However, due to the low viral load specimens and the limitations of RT-PCR, significant numbers of false negative reports are inevitable, which results in failure to timely diagnose, cut off transmission, and assess discharge criteria. To improve this situation, an optimized droplet digital PCR (ddPCR) was used for detection of SARS-CoV-2, which showed that the limit of detection...

A polyoxyethylene sorbitan oleate modified hollow gold nanoparticle system to escape macrophage phagocytosis designed for triple combination lung cancer therapy via LDL-R mediated endocytosis

Yan Shen, Yun Xia, Ershuang Yang, Zixuan Ye, Yuan Ding, Jiasheng Tu, Yong Zhang & Pengcheng Xu
Presently, a combination of chemotherapy, radiotherapy, thermotherapy, and other treatments has become a hot topic of research for the treatment of cancer, especially lung cancer. In this study, novel hollow gold nanoparticles (HGNPs) were used as drug carriers, and in order to improve the targeting ability of HGNPs to a lung tumor site, polyoxyethylene sorbitol oleate (PSO) was chosen here as a target ligand since it can be specifically recognized by the low-density lipoprotein (LDL)...

Viral RNA level, serum antibody responses, and transmission risk in recovered COVID-19 patients with recurrent positive SARS-CoV-2 RNA test results: a population-based observational cohort study

Chao Yang, Min Jiang, Xiaohui Wang, Xiujuan Tang, Shisong Fang, Hao Li, Le Zuo, Yixiang Jiang, Yifan Zhong, Qiongcheng Chen, Chenli Zheng, Lei Wang, Shuang Wu, Weihua Wu, Hui Liu, Jing Yuan, Xuejiao Liao, Zhen Zhang, Xiaolu Shi, Yijie Geng, Huan Zhang, Huanying Zheng, Min Wan, Linying Lu, Xiaohu Ren … & Qinghua Hu
Managing recovered COVID-19 patients with recurrent-positive SARS-CoV-2 RNA test results is challenging. We performed a population-based observational study to characterize the viral RNA level and serum antibody responses in recurrent-positive patients and evaluate their viral transmission risk. Of 479 recovered COVID-19 patients, 93 (19%) recurrent-positive patients were identified, characterized by younger age, with a median discharge-to-recurrent-positive length of 8 days. After readmission, recurrent-positive patients exhibited mild (28%) or absent (72%) symptoms, with no disease progression....

Viral RNA level, serum antibody responses, and transmission risk in recovered COVID-19 patients with recurrent positive SARS-CoV-2 RNA test results: a population-based observational cohort study

Chao Yang, Min Jiang, Xiaohui Wang, Xiujuan Tang, Shisong Fang, Hao Li, Le Zuo, Yixiang Jiang, Yifan Zhong, Qiongcheng Chen, Chenli Zheng, Lei Wang, Shuang Wu, Weihua Wu, Hui Liu, Jing Yuan, Xuejiao Liao, Zhen Zhang, Xiaolu Shi, Yijie Geng, Huan Zhang, Huanying Zheng, Min Wan, Linying Lu, Xiaohu Ren … & Qinghua Hu
Managing recovered COVID-19 patients with recurrent-positive SARS-CoV-2 RNA test results is challenging. We performed a population-based observational study to characterize the viral RNA level and serum antibody responses in recurrent-positive patients and evaluate their viral transmission risk. Of 479 recovered COVID-19 patients, 93 (19%) recurrent-positive patients were identified, characterized by younger age, with a median discharge-to-recurrent-positive length of 8 days. After readmission, recurrent-positive patients exhibited mild (28%) or absent (72%) symptoms, with no disease progression....

Supplemental Material, CONSORT_a - Effectiveness of Continuous Positive Airway Pressure Versus Mandibular Advancement Device in Severe Obstructive Sleep Apnea Patients With Mandibular Retrognathia: A Prospective Clinical Trial

Haichun Lai, Wei Huang, Wei Chen & Desheng Wang
Supplemental Material, CONSORT_a for Effectiveness of Continuous Positive Airway Pressure Versus Mandibular Advancement Device in Severe Obstructive Sleep Apnea Patients With Mandibular Retrognathia: A Prospective Clinical Trial by Haichun Lai, Wei Huang, Wei Chen and Desheng Wang in Ear, Nose & Throat Journal

Prolonged shedding of severe acute respiratory syndrome coronavirus 2 in patients with COVID-19

Qian Li, Xiao-Shuang Zheng, Xu-Rui Shen, Hao-Rui Si, Xi Wang, Qi Wang, Bei Li, Wei Zhang, Yan Zhu, Ren-Di Jiang, Kai Zhao, Hui Wang, Zheng-Li Shi, Hui-Lan Zhang, Rong-Hui Du & Peng Zhou
Following acute infection, individuals COVID-19 may still shed SARS-CoV-2 RNA. However, limited information is available regarding the active shedding period or whether infectious virus is also shed. Here, we monitored the clinical characteristics and virological features of 38 patients with COVID-19 (long-term carriers) who recovered from the acute disease, but still shed viral RNA for over 3 months. The median carrying history of the long-term carriers was 92 days after the first admission, and the...

Prolonged shedding of severe acute respiratory syndrome coronavirus 2 in patients with COVID-19

Qian Li, Xiao-Shuang Zheng, Xu-Rui Shen, Hao-Rui Si, Xi Wang, Qi Wang, Bei Li, Wei Zhang, Yan Zhu, Ren-Di Jiang, Kai Zhao, Hui Wang, Zheng-Li Shi, Hui-Lan Zhang, Rong-Hui Du & Peng Zhou
Following acute infection, individuals COVID-19 may still shed SARS-CoV-2 RNA. However, limited information is available regarding the active shedding period or whether infectious virus is also shed. Here, we monitored the clinical characteristics and virological features of 38 patients with COVID-19 (long-term carriers) who recovered from the acute disease, but still shed viral RNA for over 3 months. The median carrying history of the long-term carriers was 92 days after the first admission, and the...

Immune restoration in HIV-1-infected patients after 12 years of antiretroviral therapy: a real-world observational study

Jiaye Liu, Lifeng Wang, Yuying Hou, Yan Zhao, Zhihui Dou, Ye Ma, Dawei Zhang, Yasong Wu, Decai Zhao, Zhongfu Liu, Fujie Zhang, Lei Jin, Ji-Yuan Zhang, Ruonan Xu, Ming Shi, Lei Huang, Zunyou Wu, Mengjie Han, George F. Gao & Fu-Sheng Wang
Using normalization of CD4 counts as the main evaluation parameter of complete immune restoration for HIV-1 patients under antiretroviral therapy (ART) might be not enough. A comprehensive evaluation system more accurately reflecting immune restoration are urgently needed. Totally, 91,805 HIV-1 patients from 17 tertiary hospitals in China during 2005–2018 were included in this study. Immune restoration and mortality were assessed. Patients initiated ART with baseline CD4 counts <50, 50–199, 200–349, 350–499, and ≥500 cells/μL, and...

Immune restoration in HIV-1-infected patients after 12 years of antiretroviral therapy: a real-world observational study

Jiaye Liu, Lifeng Wang, Yuying Hou, Yan Zhao, Zhihui Dou, Ye Ma, Dawei Zhang, Yasong Wu, Decai Zhao, Zhongfu Liu, Fujie Zhang, Lei Jin, Ji-Yuan Zhang, Ruonan Xu, Ming Shi, Lei Huang, Zunyou Wu, Mengjie Han, George F. Gao & Fu-Sheng Wang
Using normalization of CD4 counts as the main evaluation parameter of complete immune restoration for HIV-1 patients under antiretroviral therapy (ART) might be not enough. A comprehensive evaluation system more accurately reflecting immune restoration are urgently needed. Totally, 91,805 HIV-1 patients from 17 tertiary hospitals in China during 2005–2018 were included in this study. Immune restoration and mortality were assessed. Patients initiated ART with baseline CD4 counts <50, 50–199, 200–349, 350–499, and ≥500 cells/μL, and...

Help-seeking preferences among Chinese college students exposed to a natural disaster: a person-centered approach

Wei Shi & Brian J. Hall
Direct exposure to natural disasters is associated with increased mental disorders. Help-seeking behaviour among Chinese adults is low and the barriers and facilitators of help-seeking among Chinese adults exposed to natural disasters is understudied. Using a person-centred approach, this study describes help-seeking preferences and their correlates in a sample of Chinese college students after experiencing Typhoon Hato, the strongest storm to affect Macao, China in the past 50 years. The baseline sample was collected one...

Ezrin is essential for the entry of Japanese encephalitis virus into the human brain microvascular endothelial cells

Yanang Liu, Yang Chen, Xiaohang Wang, Ping Zhao, Yongzhe Zhu & Zhongtian Qi
Japanese encephalitis virus (JEV) remains the predominant cause of viral encephalitis worldwide. It reaches the central nervous system upon crossing the blood–brain barrier through pathogenic mechanisms that are not completely understood. Here, using a high-throughput siRNA screening assay combined with verification experiments, we found that JEV enters the primary human brain microvascular endothelial cells (HBMEC) through a caveolae-mediated endocytic pathway. The role of ezrin, an essential host factor for JEV entry based on our screening,...

Ezrin is essential for the entry of Japanese encephalitis virus into the human brain microvascular endothelial cells

Yan-Gang Liu, Yang Chen, Xiaohang Wang, Ping Zhao, Yongzhe Zhu & Zhongtian Qi
Japanese encephalitis virus (JEV) remains the predominant cause of viral encephalitis worldwide. It reaches the central nervous system upon crossing the blood–brain barrier through pathogenic mechanisms that are not completely understood. Here, using a high-throughput siRNA screening assay combined with verification experiments, we found that JEV enters the primary human brain microvascular endothelial cells (HBMEC) through a caveolae-mediated endocytic pathway. The role of ezrin, an essential host factor for JEV entry based on our screening,...

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