288 Works

Two new N-acetyl-ᴅ-glucosamine derivatives from the medical algae-derived endophytic fungus Penicillium chrysogenum

Jing Chen, Li-Na Huo, Yuan Gao, Yun-Li Zhang & Ye Chen
Two new N-acetyl-ᴅ-glucosamine derivatives, penichryfurans A (1) and B (2), were obtained from the fermentation of an endophytic fungus Penicillium chrysogenum which inhabited the marine medical red alga Grateloupia turuturu. Their structures were established by detailed spectroscopic analysis of 1D and 2D NMR in combination with HRESIMS data. The absolute configurations were determined by the (Mo2(OAc)4)-induced CD and comparison of the calculated and experimental electronic curcular dichroism (ECD) spectra. Both compounds were evaluated for their...

Lignans and phenylpropanoids from the roots of Ficus hirta and their cytotoxic activities

Xian-Sheng Ye, Wen-Jing Tian, Xiang-Zhong Liu, Mi Zhou, De-Quan Zeng, Ting Lin, Guang-Hui Wang, Xin-Sheng Yao & Hai-Feng Chen
One undescribed lignan, one new natural product, along with fourteen known compounds, were isolated from the roots of Ficus hirta. The structures of the isolates were elucidated by comprehensive spectroscopic technologies, including UV, IR, HRESIMS, and NMR. The absolute configuration of 1 was determined by comparison of experimental and calculated ECD data. The cytotoxicity of all the compounds against HeLa and HepG2 cell lines was evaluated and compound 7 showed considerable cytotoxic effect towards HepG2...

Four new secoiridoids from the stem barks of Syringa reticulata (Bl.) Hara

Shuang Guo, Yan Liu, Yan-Ping Sun, Juan Pan, Wei Guan, Xiao-Mao Li, Si-Yi Wang, Adnan Mohammed Algradi, Bing-You Yang & Hai-Xue Kuang
Four new secoiridoids, syrretoside A (1), syrretoside B (2), 5β, 8β-syrretaglucone C(3), 5β, 8α-syrretaglucone C (4), together with eight known secoiridoids (5–12), were isolated from the stem barks of Syringa reticulata (Bl.) Hara. The structures of isolated compounds were established based on the physical and chemical means, NMR spectroscopy, high-resolution mass spectrometry (HR-ESI-MS), and circular dichroism spectrum (CD), as well as in comparison with the literature. The cytotoxicity of isolated compounds was investigated using CCK8...

Preparation and characterization of young Prunus persica fruit fraction and its anti-inflammatory effect on a transgenic zebrafish model

Xuanming Zhang, Lizhen Wang, Xiaobin Li, Shanshan Zhang, Hairong Hou, Mengqi Zhang, Qiuxia He & Kechun Liu
Young Prunus persica fruits (YPF) contain substances that are distinct from those found in the mature fruits. Response surface methodology was used to explore the influences of extraction conditions including ultrasonic time (X1), ethanol proportion (X2), liquid-to-solid ratio (X3) and temperature (X4) on UV-absorbing components from YPF. To purify the extract, the adsorption/desorption properties of 280 nm-absorbing components on AB-8 resin were investigated. A total of 11 metabolites (amino acids, glycosylated amino acids and phenolics)...

Structure, antioxidant property and protection on PC12 of a polysaccharide isolated and screened from Abelmoschus esculentus L.Moench (okra)

Li-Chao Pan, Yang-Yang Sun, Xiao-Ling Zhang, Zhen-Yuan Zhu, Chun-Yu Liu, Hui-Qing Sun, Xue-Qing Geng, Wei Jiang & Jia-He Wang
AeP-P-2, a pectic polysaccharide, was extracted from the fruit pod of okra. It composed of rhamnose (Rha), arabinose (Ara), glucose (Glc), galactose (Gal) and galacturonic acid (GalA) with the ratio of 4.75:2.01:1.00:4.91:7.24. The main structural feature of AeP-P-2 are 1,4-linked galacturonan units (homogalacturonan backbone) and (1 → 2) and (1 → 2,4) linked Rha (rhamnogalacturonan I region). And the other side chains contained →1)-linked Ara, (1 → 5)-linked Ara, (1 → 4)-linked Glc, (1 →...

Periconceptional folate and gestational diabetes mellitus: a systematic review and meta-analysis of cohort studies

Qingyun Liu, Shanshan Wei, Jiafan Lei, Liangping Luo & Feng Wang
To examine the relationship between periconceptional folate exposure and risk of gestational diabetes mellitus (GDM). Several electronic databases, including PubMed, Embase, China National Knowledge Infrastructure (CNKI), China Biology Medicine (CBM), and Cochrane Library, were searched for all relevant cohort studies by January 2021. Studies on relationship between folate exposure (intake or status) and GDM risk were included. Quality of included studies was assessed using Newcastle-Ottawa Scale. Random effects meta-analysis was performed to estimate overall odds...

Upregulated HMGB1 levels in maternal–fetal interface of patients with unexplained recurrent spontaneous abortion from different sources

Jing Wang, Damin Zhu, Jiaqian Yin, Cong Ma, Xiaoqing Peng, Huijuan Zou, Yunxia Cao & Xiaofeng Xu
To investigate the expression and sources of high mobility group box 1 (HMGB1) protein in the maternal–fetal interface of patients with unexplained recurrent spontaneous abortion (URSA), and further to verify the role of HMGB1 in the etiology of URSA. 55 women at early pregnancy with URSA and 55 women undergoing selective termination of normal early pregnancy as control were included. The abortion tissues including villi and decidua were collected. The expression of HMGB1, CD45, CK7,...

In-hospital acute kidney injury and atrial fibrillation: incidence, risk factors, and outcome

Guoqin Wang, Lijiao Yang, Nan Ye, Weijing Bian, Changsheng Ma, Dong Zhao, Jing Liu, Yongchen Hao, Na Yang & Hong Cheng
The incidence and the risk factors of in-hospitalized acute kidney injury (AKI) in patients hospitalized for atrial fibrillation (AF) were unclear. The Improving Care for Cardiovascular Disease in China-AF (CCC-AF) project is an ongoing registry and quality improvement project, with 240 hospitals recruited across China. We selected 4527 patients hospitalized for AF registered in the CCC-AF from January 2015 to January 2019. Patients were divided into the AKI and non-AKI groups according to the changes...

Facile design of lidocaine-loaded polymeric hydrogel to persuade effects of local anesthesia drug delivery system: complete in vitro and in vivo toxicity analyses

Yan Li, Erxian Zhao, Li Li, Liying Bai & Wei Zhang
The principal goal of the present investigation was to enterprise new and effective drug delivery vesicle for the sustained delivery of local anesthetic lidocaine hydrochloride (LDC), using a novel combination of copolymeric hydrogel with tetrahydroxyborate (COP–THB) to improve bioactivity and therapeutic potential. To support this contention, the physical and mechanical properties, rheological characteristics, and component release of candidate formulations were investigated. An optimized formulation of COP–THB containing LDC to an upper maximum concentration of 1.5%...

TNF-induced necroptosis initiates early autophagy events via RIPK3-dependent AMPK activation, but inhibits late autophagy

Wenxian Wu, Xiaojing Wang, Yadong Sun, Niklas Berleth, Jana Deitersen, David Schlütermann, Fabian Stuhldreier, Nora Wallot-Hieke, María José Mendiburo, Jan Cox, Christoph Peter, Ann Kathrin Bergmann & Björn Stork
Macroautophagy/autophagy and necroptosis represent two opposing cellular s tress responses. Whereas autophagy primarily fulfills a cyto-protective function, necroptosis is a form of regulated cell death induced via death receptors. Here, we aimed at investigating the molecular crosstalk between these two pathways. We observed that RIPK3 directly associates with AMPK and phosphorylates its catalytic subunit PRKAA1/2 at T183/T172. Activated AMPK then phosphorylates the autophagy-regulating proteins ULK1 and BECN1. However, the lysosomal degradation of autophagosomes is blocked...

CUL3 (cullin 3)-mediated ubiquitination and degradation of BECN1 (beclin 1) inhibit autophagy and promote tumor progression

Xuan Li, Kai-Bin Yang, Wei Chen, Jia Mai, Xiao-Qi Wu, Ting Sun, Rui-Yan Wu, Lin Jiao, Dan-Dan Li, Jiao Ji, Hai-Liang Zhang, Yan Yu, Yu-Hong Chen, Gong-Kan Feng, Rong Deng, Jun-Dong Li & Xiao-Feng Zhu
Macroautophagy/autophagy plays an important role during the development of human cancer. BECN1 (beclin 1), a core player in autophagy regulation, is downregulated in many kinds of malignancy. The underlying mechanism, however, has not been fully illuminated. Here, we found that CUL3 (cullin 3), an E3 ubiquitin ligase, could interact with BECN1 and promote the K48-linked ubiquitination and degradation of this protein; In addition, CUL3 led to a decrease in autophagic activity through downregulating BECN1. We...

Two types of immune infiltrating cells and six hub genes can predict the occurrence of myasthenia gravis in patients with thymoma

Ziyou Tao, Chao Lu, Shuai Gao, Peng Zhang, Yuan Chen, Yuanguo Wang, Zhaoyu Yang, Kai Xiong & Yuxin Liu
Thymoma is the most common primary mass in anterior mediastinum. Although associated with low malignancy, it is often accompanied by myasthenia gravis resulting in poor prognosis. Due to the dual factors of tumor immune tolerance and autoimmune reaction, it is urgent to understand the immune status of MG with thymoma. In this study, RNA sequencing data were obtained from the TCGA and GEO cohorts to identify differentially expressed messenger RNAs and infiltrated immune cells. A...

Ephedrine alleviates middle cerebral artery occlusion-induced neurological deficits and hippocampal neuronal damage in rats by activating PI3K/AKT signaling pathway

Lixian Huang, Bo Zhao, Qunxian Li, Jing Wu, Hui Jiang & Qingbin Li
Inflammation and oxidative stress are crucial in ischemic stroke. Ephedrine (EPH) has been proven to have anti-inflammatory and anti-oxidative stress effects. The present study analyzes whether EPH possessed neuroprotective effects and explored the underlying mechanisms of EPH based on an experimental model of middle cerebral artery occlusion (MCAO). We found that intraperitoneal injection with EPH attenuated the neurological deficit, cerebral infarction, and cerebral edema induced by MCAO in rats. Besides, EPH treatment alleviated MCAO-induced brain...

Up-regulation of S100P predicts the poor long-term survival and construction of prognostic signature for survival and immunotherapy in patients with pancreatic cancer

Wenbo Zou, Lincheng Li, Zizheng Wang, Nan Jiang, Fei Wang, Minggen Hu & Rong Liu
Pancreatic cancer is associated with a high mortality rate, and the prognosis is positively related to immune status. In this study, we constructed a prognostic signature from survival- and immune-related genes (IRGs) to guide treatment and assess prognosis of patients with pancreatic cancer. The transcriptomic data were obtained from The Cancer Genome Atlas (TCGA) database, and IRGs were extracted from the ImmPort database. Univariate and LASSO regression analysis were used to obtain survival-related IRGs. Finally,...

Long non-coding RNA LINC00958 promotes colorectal cancer progression by enhancing the expression of LEM domain containing 1 via microRNA miR-3064-5p

Zhaoxia Luo, Shunxin Hao, Jian Yuan, Kai Zhu, Shuo Liu, Jing Zhang & Lei Yao
Colorectal cancer is a common cause of cancer-related death worldwide. Thus, there is an urgent need to determine the mechanism of progression of colorectal cancer. In this study, we investigated the function and mechanism of long non-coding RNA LINC00958, providing a new biomarker for colorectal cancer. The expression of LINC00958, miR-3064-5p, and LEM domain containing 1 (LEMD1) in colorectal cancer tissues and cell lines was analyzed using reverse transcription quantitative polymerase chain reaction (RT-qPCR). The...

Association study of hypertension susceptibility genes ITGA9, MOV10, and CACNB2 with preeclampsia in Chinese Han population

Qian Xin, Gang Xin, Li Li, Wenjuan Sun, Wen Jiang, Jue Wang, Yun Luan, Ying Zhang, Ling Cheng, Shuhong Duan, Fanzhen Hong, Qinghong Ji & Weihong Ma
Preeclampsia (PE) is a disorder that occurs during the pregnancy and could affect the maternal and perinatal mortality as well as morbidity. The aim of our study is to investigate the associations between the hypertension susceptibility genes ITGA9, MOV10 and CACNB2 with PE in Chinese Han population. A case–control study including 178 PE patients and 202 healthy controls was conducted to assess the associations between three loci (ITGA9 rs155524, MOV10 rs2932538 and CACNB2 rs4373814) and...

The safety and tolerability of alkaloids from Alstonia scholaris leaves in healthy Chinese volunteers: a single-centre, randomized, double-blind, placebo-controlled phase I clinical trial

Zhong-Ping Gou, Yun-Li Zhao, Lin-Ling Zou, Ying Wang, Shi-Qing Shu, Xiao-Hong Zhu, Li Zheng, Qi Shen, Zhu Luo, Jia Miao, Yong-Sheng Wang, Xiao-Dong Luo & Ping Feng
Capsule of alkaloids from the leaf of Alstonia scholaris (L.) R.Br. (Apocynaceae) (CALAS) is a new investigational botanical drug (No. 2011L01436) for bronchitis, post-infectious cough and asthma. To observe the clinical safety and tolerability of CALAS. Subjects were assigned to eight cohorts, and each received randomly CALAS or placebo in one of single ascending dose (SAD) of 8, 40, 120, 240, 360, 480, or in one of multiple ascending dose (MAD) of 40 or 120...

Yiguanjian decoction inhibits macrophage M1 polarization and attenuates hepatic fibrosis induced by CCl4/2-AAF

Ying Xu, Wen Xu, Wei Liu, Gaofeng Chen, Shili Jiang, Jiamei Chen, Xun Jian, Hua Zhang, Ping Liu & Yongping Mu
Our previous studies indicated that Yiguanjian decoction (YGJ) has an anti-hepatic-fibrosis effect and could regulate macrophage status. To elucidate the mechanism of YGJ in regulating macrophages. Liver cirrhosis was induced by CCl4 for 12 weeks combined with 2-acetylaminofluorene (2-AAF) for the last 4 weeks in male Wistar rats. YGJ (3.56 mg/kg) orally administered in the last 4 weeks, and SORA (1 mg/kg) as control. In vitro, RAW264.7 cells were treated with lipopolysaccharides (LPSs) to induce...

Salidroside orchestrates metabolic reprogramming by regulating the Hif-1α signalling pathway in acute mountain sickness

Xiaoning Yan, Jie Liu, Meixia Zhu, Lirong Liu, Yijun Chen, Yinhuan Zhang, Menghan Feng, Zhixin Jia & Hongbin Xiao
Rhodiola crenulata (Hook. f. et Thoms.) H. Ohba (Crassulaceae) is used to prevent and treat acute mountain sickness. However, the mechanisms underlying its effects on the central nervous system remain unclear. To investigate the effect of Rhodiola crenulata on cellular metabolism in the central nervous system. The viability and Hif-1α levels of microglia and neurons at 5% O2 for 1, 3, 5 and 24 h were examined. We performed the binding of salidroside (Sal), rhodiosin,...

Salidroside orchestrates metabolic reprogramming by regulating the Hif-1α signalling pathway in acute mountain sickness

Xiaoning Yan, Jie Liu, Meixia Zhu, Lirong Liu, Yijun Chen, Yinhuan Zhang, Menghan Feng, Zhixin Jia & Hongbin Xiao
Rhodiola crenulata (Hook. f. et Thoms.) H. Ohba (Crassulaceae) is used to prevent and treat acute mountain sickness. However, the mechanisms underlying its effects on the central nervous system remain unclear. To investigate the effect of Rhodiola crenulata on cellular metabolism in the central nervous system. The viability and Hif-1α levels of microglia and neurons at 5% O2 for 1, 3, 5 and 24 h were examined. We performed the binding of salidroside (Sal), rhodiosin,...

Targeted therapy of rheumatoid arthritis via macrophage repolarization

Xu Zhou, Dandan Huang, Runkong Wang, Mingquan Wu, Liyang Zhu, Wei Peng, He Tu, Xuangeng Deng, He Zhu, Zhong Zhang, Xinming Wang & Xi Cao
The polarization of macrophages plays a critical role in the physiological and pathological progression of rheumatoid arthritis (RA). Activated M1 macrophages overexpress folate receptors in arthritic joints. Hence, we developed folic acid (FA)-modified liposomes (FA-Lips) to encapsulate triptolide (TP) (FA-Lips/TP) for the targeted therapy of RA. FA-Lips exhibited significantly higher internalization efficiency in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells than liposomes (Lips) in the absence of folate. Next, an adjuvant-induced arthritis (AIA) rat model was established...

Β-Cyclodextrin-graft-poly(amidoamine) dendrons as the nitric oxide deliver system for the chronic rhinosinusitis therapy

Tao Liu, Guowei Li, Xidong Wu, Shaohua Chen, Siyi Zhang, Hong Han, Hongbin Zhang, Xiaoning Luo, Xiang Cai & Dong Ma
Chronic rhinosinusitis (CRS) is a rather prevalent condition with a chronic inflammatory process, which is hard to cure. Herein, a new antibacterial drug, nitric oxide (NO), was used for the attempt on CRS therapy. To achieve this, a star copolymer (β-CD-PAMAM) consisting of the β-cyclodextrin (β-CD) core and seven PAMAM-G3 arms, which was designed as a low-cytotoxicity and high NO loading carrier, were synthesized and characterizied. The obtained β-CD-PAMAM/NONOate showed the effect in inhibiting and...

Prevalence and predictors of post-traumatic stress disorder in patients with cured coronavirus disease 2019 (COVID-19) one month post-discharge

Yumeng Ju, Jin Liu, Roger M. K. Ng, Bangshan Liu, Mi Wang, Wentao Chen, Mei Huang, Aiping Yang, Kongliang Shu, Yun Zhou, Li Zhang, Mei Liao, Jiyang Liu & Yan Zhang
Background: Coronavirus disease 2019 (COVID-19) can place an immense psychological strain on the infected patient. The psychological distress can linger after the initial recovery from the infection. Objective: This study aimed to evaluate the prevalence and predictors of provisional post-traumatic stress disorder (PTSD) in patients with cured COVID-2019. Methods: The baseline survey was conducted from 10 to 25 February 2020 in patients with COVID-19 in a designated hospital. Demographic and clinical characteristics were acquired, and...

Aspirin relieves the calcification of aortic smooth muscle cells by enhancing the heat shock response

Quanquan Shen, Qian Chen, Yang Liu, Xiang Xue, Xiaogang Shen, Qiang He, Guokun Wang & Fei Han
Vascular calcification is a major complication of chronic renal failure, which has been identified as an active process partly driven by osteogenic transition of vascular smooth muscle cells (VSMCs). Aspirin could prevent cardiomyocyte damage by inducing heat shock response. This study investigates the effect of aspirin on alleviating VSMC calcification. An in vitro VSMC calcification model was established by 10-day calcification induction in osteogenic medium. VSMCs were grouped as following: control group (normal medium), calcified...

A pH-responsive complex based on supramolecular organic framework for drug-resistant breast cancer therapy

Yun-Chang Zhang, Pei-Yu Zeng, Zhi-Qiang Ma, Zi-Yue Xu, Ze-Kun Wang, Beibei Guo, Feng Yang & Zhan-Ting Li
Chemotherapy is one of the main ways to treat breast cancer clinically. However, the multidrug resistance to anti-tumor drugs limits their clinical use. To overcome these drawbacks, development of drug delivery systems (DDSs) has attracted more and more attention in cancer therapy. At present, the preparation and purification process are complicated for many reported DDSs, while clinic calls for new DDSs that are more convenient for preparation. Here, a new pH-responsive supramolecular organic framework drug...

Registration Year

  • 2021

Resource Types

  • Text


  • Huazhong University of Science and Technology
  • Shanghai Jiao Tong University
  • Chinese Academy of Medical Sciences & Peking Union Medical College
  • Sun Yat-sen University
  • Zhejiang University
  • Chinese Academy of Sciences
  • Fudan University
  • Sichuan University
  • Capital Medical University
  • Nanjing Medical University