2,775 Works

Implementation of novel boolean logic gates for IMPLICATION and XOR functions using riboregulators

Chaoxin Chen, Qi Wu, Qingying Ke, Ting Wang, Yifan Zhang, Feiwen Wei, Xiaolong Wang & Guanglei Liu
To date, several different types of synthetic genetic switches, including riboregulators, riboswitches, and toehold switches, have been developed to construct AND, OR, NOT, NAND, NOR, and NOT IMPLICATION (NIMP) gates. The logic gate can integrate multiple input signals following a set of algorithms and generate a response only if strictly defined conditions are met. However, there are still some logic gates that have not been implemented but are necessary to build complex genetic circuits. Here,...

Aetiology of severe community acquired pneumonia in adults identified by combined detection methods: a multi-centre prospective study in China

Jieming Qu, Jing Zhang, Yu Chen, Yi Huang, Yusang Xie, Min Zhou, Yuping Li, Dongwei Shi, Jinfu Xu, Qiuyue Wang, Bei He, Ning Shen, Bin Cao, Danyang She, Yi Shi, Xin Su, Hua Zhou, Hong Fan, Feng Ye, Qiao Zhang, Xinlun Tian & Guoxiang Lai
Severe Community Acquired Pneumonia (SCAP) challenges public health globally. Considerable improvements in molecular pathogen testing emerged in the last few years. Our prospective study combinedly used traditional culture, antigen tests, PCR and mNGS in SCAP pathogen identification with clinical outcomes. From June 2018 to December 2019, we conducted a multi-centre prospective study in 17 hospitals of SCAP patients within 48 hours of emergency room stay or hospitalization in China. All clinical data were uploaded into...

Aetiology of severe community acquired pneumonia in adults identified by combined detection methods: a multi-centre prospective study in China

Jieming Qu, Jing Zhang, Yu Chen, Yi Huang, Yusang Xie, Min Zhou, Yuping Li, Dongwei Shi, Jinfu Xu, Qiuyue Wang, Bei He, Ning Shen, Bin Cao, Danyang She, Yi Shi, Xin Su, Hua Zhou, Hong Fan, Feng Ye, Qiao Zhang, Xinlun Tian & Guoxiang Lai
Severe Community Acquired Pneumonia (SCAP) challenges public health globally. Considerable improvements in molecular pathogen testing emerged in the last few years. Our prospective study combinedly used traditional culture, antigen tests, PCR and mNGS in SCAP pathogen identification with clinical outcomes. From June 2018 to December 2019, we conducted a multi-centre prospective study in 17 hospitals of SCAP patients within 48 hours of emergency room stay or hospitalization in China. All clinical data were uploaded into...

Short divalent ethacrynic amides as pro-inhibitors of glutathione S-transferase isozyme Mu and potent sensitisers of cisplatin-resistant ovarian cancers

Bangtian Xu, Tingting Tong, Xin Wang, Fang Liu, Xiang Zhang, Xiaolei Hu, Xinpeng Li, Xiaolan Yang & Fei Liao
The linking of ethacrynic acid with ethylenediamine and 1,4-butanediamine gave EDEA and BDEA, respectively, as membrane-permeable divalent pro-inhibitors of glutathione S-transferase (GST). Their divalent glutathione conjugates showed subnanomolar inhibition and divalence-binding to GSTmu (GSTM) (PDB: 5HWL) at ∼0.35 min−1. In cisplatin-resistant SK-OV-3, COC1, SGC7901 and A549 cells, GSTM activities probed by 15 nM BDEA or EDEA revealed 5-fold and 1.0-fold increases in cisplatin-resistant SK-OV-3 and COC1 cells, respectively, in comparison with the susceptible parental cells....

Short divalent ethacrynic amides as pro-inhibitors of glutathione S-transferase isozyme Mu and potent sensitisers of cisplatin-resistant ovarian cancers

Bangtian Xu, Tingting Tong, Xin Wang, Fang Liu, Xiang Zhang, Xiaolei Hu, Xinpeng Li, Xiaolan Yang & Fei Liao
The linking of ethacrynic acid with ethylenediamine and 1,4-butanediamine gave EDEA and BDEA, respectively, as membrane-permeable divalent pro-inhibitors of glutathione S-transferase (GST). Their divalent glutathione conjugates showed subnanomolar inhibition and divalence-binding to GSTmu (GSTM) (PDB: 5HWL) at ∼0.35 min−1. In cisplatin-resistant SK-OV-3, COC1, SGC7901 and A549 cells, GSTM activities probed by 15 nM BDEA or EDEA revealed 5-fold and 1.0-fold increases in cisplatin-resistant SK-OV-3 and COC1 cells, respectively, in comparison with the susceptible parental cells....

Stachydrine is effective and selective against blast phase chronic myeloid leukaemia through inhibition of multiple receptor tyrosine kinases

Ruixin Gu, Wei Zhang & Dandan Xu
Resistance to BCR-ABL tyrosine kinase inhibitor (TKI) is the cause of treatment failure in blast phase chronic myeloid leukaemia (BP-CML). Agents that act synergistically with BCR-ABL TKI are required to improve response. This work investigated the effects of stachydrine in CML. CML cells were treated with control or stachydrine at 20, 40 and 80 µM. Proliferation and apoptosis were examined after 72 h treatment. Combination studies were performed in four groups: control, TKI, stachydrine and...

The path to diagnosis in ALS: delay, referrals, alternate diagnoses, and clinical progression

Stefan Sennfält, Ulf Kläppe, Sebastian Thams, Kristin Samuelsson, Rayomand Press, Fang Fang & Caroline Ingre
Objective: To provide a detailed and differentiated description of the path to receiving the correct amyotrophic lateral sclerosis (ALS) diagnosis, including delay times, referrals, alternate diagnoses, and clinical progression. Methods: Medical records until the date of ALS diagnosis were reviewed and linked to the Swedish Motor Neuron Disease Quality Registry. Results: The study included 353 Stockholm ALS patients diagnosed in 2016–2021. Patients were divided into four groups: 117 (33.1%) with lower extremity (LE), 85 (24.1%)...

The path to diagnosis in ALS: delay, referrals, alternate diagnoses, and clinical progression

Stefan Sennfält, Ulf Kläppe, Sebastian Thams, Kristin Samuelsson, Rayomand Press, Fang Fang & Caroline Ingre
Objective: To provide a detailed and differentiated description of the path to receiving the correct amyotrophic lateral sclerosis (ALS) diagnosis, including delay times, referrals, alternate diagnoses, and clinical progression. Methods: Medical records until the date of ALS diagnosis were reviewed and linked to the Swedish Motor Neuron Disease Quality Registry. Results: The study included 353 Stockholm ALS patients diagnosed in 2016–2021. Patients were divided into four groups: 117 (33.1%) with lower extremity (LE), 85 (24.1%)...

Identification of HPCAL1 as a specific autophagy receptor involved in ferroptosis

Xin Chen, Xinxin Song, Jingbo Li, Ruoxi Zhang, Chunhua Yu, Zhuan Zhou, Jiao Liu, Siyan Liao, Daniel J. Klionsky, Guido Kroemer, Jinbao Liu, Daolin Tang & Rui Kang
Selective macroautophagy/autophagy maintains cellular homeostasis through the lysosomal degradation of specific cellular proteins or organelles. The pro-survival effect of selective autophagy has been well-characterized, but the mechanism by which it drives cell death is still poorly understood. Here, we use a quantitative proteomic approach to identify HPCAL1 (hippocalcin like 1) as a novel autophagy receptor for the selective degradation of CDH2 (cadherin 2) during ferroptosis. HPCAL1-dependent CDH2 depletion increases susceptibility to ferroptotic death by reducing...

Discovery of novel sulphonamide hybrids that inhibit LSD1 against bladder cancer cells

Jia Liu, Xingwang Zhu, Liu Yu & Minghuan Mao
Aim:A series of sulphonamide hybrids were designed, synthesised, and identified as potential lysine-specific demethylase 1 (LSD1) inhibitors. Materials and methods: Bladder cancer cell lines were cultured to evaluate the antiproliferative activity. Inhibitory evaluation of sulphonamide hybrids against LSD1 were performed. Conclusion: sulphonamide derivative L8 exhibited the antiproliferative activity against HTB5, HTB3, HT1376, and HTB1 cells with IC50 values of 1.87, 0.18, 0.09, and 0.93 μM, respectively. Compound L8 as a selective and reversible LSD1 inhibitor...

Discovery of novel sulphonamide hybrids that inhibit LSD1 against bladder cancer cells

Jia Liu, Xingwang Zhu, Liu Yu & Minghuan Mao
Aim:A series of sulphonamide hybrids were designed, synthesised, and identified as potential lysine-specific demethylase 1 (LSD1) inhibitors. Materials and methods: Bladder cancer cell lines were cultured to evaluate the antiproliferative activity. Inhibitory evaluation of sulphonamide hybrids against LSD1 were performed. Conclusion: sulphonamide derivative L8 exhibited the antiproliferative activity against HTB5, HTB3, HT1376, and HTB1 cells with IC50 values of 1.87, 0.18, 0.09, and 0.93 μM, respectively. Compound L8 as a selective and reversible LSD1 inhibitor...

Timosaponin B-II alleviates osteoarthritis-related inflammation and extracellular matrix degradation through inhibition of mitogen-activated protein kinases and nuclear factor-κB pathways in vitro

Xinwei Liu, Dulei Xiang, Wenming Jin, Gen Zhao, Han Li, Bing Xie & Xiaochuan Gu
Osteoarthritis (OA), an inflammatory response in chondrocytes, leads to extracellular matrix (ECM) degradation and cartilage destruction. Timosaponin B-II (TB-II) is the main bioactive component of Rhizoma Anemarrhenae with reported antioxidant and anti-inflammatory effects. This study investigated the anti-OA function and mechanism of TB-II on IL-1β-stimulated SW1353 cells and primary rat chondrocytes. We firstly screened the concentration of TB-II in SW1353 cells and primary rat chondrocytes using CCK-8 assay. Thereafter, SW1353 cells and chondrocytes were, respectively,...

Identification of biomarkers for early diagnosis of multiple myeloma by weighted gene co-expression network analysis and their clinical relevance

Mengling Xu, Ye Meng, Qian Li, Alice Charwudzi, Hui Qin & Shudao Xiong
Multiple myeloma is an incurable hematologic malignancy, its early diagnosis is important. However, the biomarker for early diagnosis is limited; hence more need to be identified. The present study aimed to explore the easily tested new biomarker in multiple myeloma by weighted gene co-expression network analysis (WGCNA). Differentially expressed genes (DEGs) were screened using GSE47552. WGCNA was used to screen hub genes. Subsequently. Hub genes of multiple myeloma were obtained by intersection of DEGs and...

Aspartate aminotransferase-to-platelet ratio index for predicting late recurrence of hepatocellular carcinoma after radiofrequency ablation

Xinyuan Zhang, Yujing Xin, Yi Yang, Yi Chen, Xiao-jing Cao, Yanan Wang, Qingsheng Fan, Xiang Zhou & Xiao Li
Our study aimed to explore the prognostic value of the aspartate aminotransferase–platelet ratio index (APRI) and to develop a new nomogram for patients with hepatocellular carcinoma (HCC) who experience late recurrence after radiofrequency ablation (RFA). To date, no study has explored the value of APRI for assessing the late recurrence of HCC after RFA. The prognostic value of APRI was evaluated and validated in our multicenter retrospective analysis. A total of 466 HCC patients undergoing...

Aspartate aminotransferase-to-platelet ratio index for predicting late recurrence of hepatocellular carcinoma after radiofrequency ablation

Xinyuan Zhang, Yujing Xin, Yi Yang, Yi Chen, Xiao-jing Cao, Yanan Wang, Qingsheng Fan, Xiang Zhou & Xiao Li
Our study aimed to explore the prognostic value of the aspartate aminotransferase–platelet ratio index (APRI) and to develop a new nomogram for patients with hepatocellular carcinoma (HCC) who experience late recurrence after radiofrequency ablation (RFA). To date, no study has explored the value of APRI for assessing the late recurrence of HCC after RFA. The prognostic value of APRI was evaluated and validated in our multicenter retrospective analysis. A total of 466 HCC patients undergoing...

Analysis of the genetic diversity of grain legume germplasm resources in China and the development of universal SSR primers

Runnan Zhou, Siqi Yang, Bo Zhang, Zhaoming Qi, Dawei Xin, Anyu Su, Sinan Li, Peng Cheng, Yunqi Bai, Zhengong Yin, Binshuo Zhang, Yujing Zhao, Ying Zhao, Qingshan Chen & Xiaoxia Wu
The research objects of this paper are Vigna angularis, Vigna radiata and Phaseolus vulgaris, which are important grain legume resources in China. However, the genetic structure between the three species is not clear. Therefore, this study developed a set of universal gSSR (simple sequence repeat) primers based on the whole-genome sequences of adzuki beans, mung beans and common beans and analysed the genetic diversity of the test resources based on universal gSSR loci. A total...

Analysis of the genetic diversity of grain legume germplasm resources in China and the development of universal SSR primers

Runnan Zhou, Siqi Yang, Bo Zhang, Zhaoming Qi, Dawei Xin, Anyu Su, Sinan Li, Peng Cheng, Yunqi Bai, Zhengong Yin, Binshuo Zhang, Yujing Zhao, Ying Zhao, Qingshan Chen & Xiaoxia Wu
The research objects of this paper are Vigna angularis, Vigna radiata and Phaseolus vulgaris, which are important grain legume resources in China. However, the genetic structure between the three species is not clear. Therefore, this study developed a set of universal gSSR (simple sequence repeat) primers based on the whole-genome sequences of adzuki beans, mung beans and common beans and analysed the genetic diversity of the test resources based on universal gSSR loci. A total...

A novel genetic variant potentially altering the expression of MANBA in the cerebellum associated with attention deficit hyperactivity disorder in Han Chinese children

Xinzhen Chen, Ting Yao, Jinliang Cai, Qi Zhang, Shanyawen Li, Huiru Li, Xihang Fu & Jing Wu
To obtain additional insight into the genetic factors of attention deficit hyperactivity disorder (ADHD). First, we performed a transcriptome-wide association study (TWAS) integrating human cerebellum-specific variant-expression/splicing correlations to identify ADHD susceptibility genes. Then, the associations between expression/splicing quantitative trait loci (eQTLs/sQTLs) of the transcriptome-wide significant genes and ADHD were observed in a case-control study of Han Chinese children. Furthermore, dual luciferase reporter gene assays were performed to validate the regulatory function of ADHD risk variants....

Identification of circular RNAs and functional competing endogenous RNA networks in human proximal tubular epithelial cells treated with sodium-glucose cotransporter 2 inhibitor dapagliflozin in diabetic kidney disease

Yi Song, Feng Guo, Yifan Liu, Fengjuan Huang, Xunjie Fan, Lin Zhao & Guijun Qin
Diabetic kidney disease (DKD) is a serious diabetes complication. Sodium-glucose cotransporter 2 inhibitors (SGLT2i) are novel anti-diabetes drugs that confer clinical renal protection. However, the molecular mechanisms involved remain unclear. Here, human proximal tubular epithelial cells (PTECs) were treated with normal glucose, high glucose, and anti-diabetes agents, including SGLT2i (dapagliflozin), metformin, and dipeptidyl peptidase-4 inhibitor (DPP-4i, vildagliptin) and microarray analysis was performed. Firstly, a total of 2,710 differentially expressed circular RNAs (circRNAs) were identified. Secondly,...

Association between Tumor Necrosis factor-Alpha(TNF-a) polymorphisms and Schizophrenia: an updated meta-analysis

Sidi He, Lei Zhang, Shunying Yu, Wenjuan Yu, Yimin Yu, Jingjing Huang & Huafang Li
Previous studies have explored associations between Tumour Necrosis factor-Alpha (TNF-a) polymorphisms and Schizophrenia. Their results were controversial. We conducted a meta-analysis to clarify the association between TNF-a − 308 G/A(rs1800629), −1031T/C(rs1799964), −863C/A(rs1800630) and −857 C/T (rs1799724) polymorphisms and Schizophrenia. All the studies that investigated the association between TNF-a polymorphisms and Schizophrenia published before 15 October 2020 were included in. The literature were comprehensively searched and identified in 2 English databases and 2 Chinese databases. The...

Transcriptomic analysis of the innate immune signatures of a SARS-CoV-2 protein subunit vaccine ZF2001 and an mRNA vaccine RRV

Qian Wang, Ziyang Song, Jinghuan Yang, Qian He, Qunying Mao, Yu Bai, Jianyang Liu, Chaoqiang An, Xujia Yan, Bopei Cui, Lifang Song, Dong Liu, Miao Xu & Zhenglun Liang
Analysis of large-scale gene expression post vaccination can provide an overview of immune responses. We used transcriptional approaches to comprehensively analyze the innate immune response signatures elicited by protein subunit (PS) vaccine ZF2001 and an mRNA vaccine named RRV. A fine-grained time-dependent dissection of large-scale gene expression post immunization revealed that ZF001 induced MHC class II-related genes, including cd74 and H2-Aa, more expeditiously than the RRV. Notably, the RRV induced MHC class I-related genes such...

PARP12 is required for mitochondrial function maintenance in thermogenic adipocytes

Fan Hu, Chang Li, Yafen Ye, Xuhong Lu, Miriayi Alimujiang, Ningning Bai, Jingjing Sun, Xiaojing Ma, Xiaohua Li & Ying Yang
PARP12 is a member of poly-ADP-ribosyl polymerase (PARPs), which has been characterized for its antiviral function. Yet its physiological implication in adipocytes remains unknown. Here, we report a central function of PARP12 in thermogenic adipocytes. We show that PARP12 is highly expressed in brown adipose tissue and is mainly localized to the mitochondria. Knockdown of PARP12 in vitro reduced UCP1 expression. In parallel, the deficiency of PARP12 reduced mitochondrial respiration in adipocytes, while overexpression of...

Nitric oxide generated by Piriformospora indica-induced nitrate reductase promotes tobacco growth by regulating root architecture and ammonium and nitrate transporter gene expression

Han Li, Shenghua Fu, Jingwei Zhu, Weichang Gao, Lin Chen, Xiang Li, Shaoyu Zhang, Sha Zheng, Heng Zhang & Yanxia Liu
Nitric oxide (NO) is involved not only in the regulation of plant growth, development, and stress responses but also in the regulation of plant-microbe interactions. Here, we demonstrate that Piriformospora indica can induce tobacco nitrate reductase to produce a NO signal in roots which enhances nitrogen uptake capacity by inducing the expression of ammonium and nitrate transporter genes and the development of lateral root and root hair, thereby promoting tobacco growth. In addition, the NO...

Catalytic performances of fluorine-doped graphene/Pb composite for thermal decomposition of ammonium perchlorate

Qiang Ling, Hanwen Liang, Rong Wu, Zhihao Wang, Lei Zhao, Qingping Ke, Xiangchun Liu & Ping Cui
Fluorine-doped graphene supported PbO (F-rGO/PbO) composite was prepared through a facile water bath method to study an improvement strategy for its catalytic performance in thermal decomposition of ammonium perchlorate (AP). The characterization results showed that the PbO with the size of 20 ∼ 30 nm are highly dispersed on the surface of F-doped graphene (F-rGO). Compared with other samples, the F-rGO/PbO exhibited superior catalytic performance, as 3 wt% of catalyst added in AP, the peak...

A short report of novel RARG-HNRNPM fusion gene in resembling acute promyelocytic leukemia

Yang Song, Jiangxue Hou, Li Wan, Kaiqi Liu, Chunlin Zhou, Shuning Wei, Guangji Zhang, Dong Lin, Yan Li, Qiuyun Fang, Yuntao Liu, Benfa Gong, Xiaoyuan Gong, Ying Wang, Hui Wei, Jianxiang Wang & Yingchang Mi
Resembling acute promyelocytic leukemia (APL) is a unique subtype of APL who sharing clinical, morphological, and immunophenotypic features with typical APL, but lacking evidence of PML-RARA fusion gene and usually insensitive to arsenic trioxide (ATO) and all-trans retinoic acid (ATRA). For years, RARA, RARB and RARG rearrangement were found in resembling APL continually. The confirmed partner genes of RARG rearrangement included CPSF6, NUP98, NPM1, PML, and HNRNPC. These patients were a group of resembling APL...

Registration Year

  • 2022
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Affiliations

  • Huazhong University of Science and Technology
    2,775
  • Chinese Academy of Medical Sciences & Peking Union Medical College
    1,870
  • Shanghai Jiao Tong University
    1,699
  • Fudan University
    1,571
  • Zhejiang University
    1,565
  • Sichuan University
    1,412
  • Sun Yat-sen University
    1,339
  • Capital Medical University
    1,335
  • Nanjing Medical University
    1,293
  • Chinese Academy of Sciences
    1,258