A phytochemical investigation of Bidens procera L.C.Xu ex X.W.Zheng afforded two novel polyacetylenes, tridecane-2E-monoene-4,6,8-triyntylen-1,13-diol-12-O-β-glucoside (1) and tetradecane-2E,8E-diene-4,6-diyne-1,14-diol-13-O-β-glucoside (2), together with ten known compounds (3 − 12). Their chemical structures were elucidated by NMR and MS spectrums as well as the comparison of the published data. Furthermore, the chemotaxonomy of the yielded compounds was also discussed.

Additional file 3: Supplemental Table S3. Plasmid sequence of PCSK9

Additional file 6.

Additional file 1.

Patients with depression have a high prevalence of developing dyslipidemia. In this study, we aim to investigate the difference of serum lipids, including total cholesterol (TCH), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), and triglycerides (TG), between the depressed patients and healthy controls. Sex differences in lipids and their psychological correlations were also included. The study included 56 healthy controls (males/females = 26/30) and 110 first-diagnosed drug-naïve outpatients (males/females = 35/75). A total of...

A new dihydroflavone, 2(S)-isookanin-4′-methoxy-8-O-β-D-glucopyranoside (1), and a new polyacetylene glucoside, (10S)-tridecane-2E-ene-4,6,8-triyne-1-ol-10-O-β-D-glucopyranoside (2), along with seven known compounds (3–9), were isolated from the herb of Bidens parviflora Willd. The structures of all the extracted compounds were elucidated by HR-ESI-MS, 1 D and 2 D NMR spectra, as well as circular dichroism (CD).

Numerous methods based on square rasters have been proposed for polygon generalization. However, these methods ignore the inconsistent distance measurement among neighborhoods of squares, which may result in an imbalanced generalization in different directions. As an alternative raster, a hexagon has consistent connectivity and isotropic neighborhoods. This study proposed a hexagon-based method for polygon generalization using morphological operators. First, we defined three generalization operators: aggregation, elimination, and line simplification, based on hexagonal morphological operations. We...

Additional file1: Fig. S1. OUM1 knockdown impairs UM cell metastasis and proliferation. Fig. S2. GO and pathway function analysis. Fig. S3. Bioinformatics analysis of OUM1 targets. Fig. S4. PTPRZ1 might be a target of OUM1. Fig. S5. Synergistic effect of SOV and OUM1 interference on apoptosis. Fig. S6. In vivo toxicity evaluation. Table S1. Downregulated and Upregulated gene. Table S2. Primers and siRNA used in this study. Table S3. Primers used in RNA-ChIP assay. Details...

Research on microcapsules has been conducted in recent years given trends in miniaturization and novel functionalization. In this work, we designed and prepared a series of unique shape memory polyurethane (SMPU) microcapsules with stimuli-responsive functions. The microcapsule has a core-shell structure in which the surface morphology can be adjusted, and it has a certain load-bearing capacity. In addition, the SMPU microcapsule has a stimuli-responsive function for shape memory and solvent response. The temperature of its...

A phytochemical investigation of Bidens procera L.C.Xu ex X.W.Zheng afforded two novel polyacetylenes, tridecane-2E-monoene-4,6,8-triyntylen-1,13-diol-12-O-β-glucoside (1) and tetradecane-2E,8E-diene-4,6-diyne-1,14-diol-13-O-β-glucoside (2), together with ten known compounds (3 − 12). Their chemical structures were elucidated by NMR and MS spectrums as well as the comparison of the published data. Furthermore, the chemotaxonomy of the yielded compounds was also discussed.

Additional file 4: Supplemental Table S4. Primers used for qRT- PCR

Additional file 2: Supplemental Table S2. ThesiRNA sequences of NC and siPCSK9

Supplemental Material for Pretreatment Serum Lactate Dehydrogenase and Metastases Numbers as Potential Determinants of Anti-PD-1 Therapy Outcome in Nasopharyngeal Carcinoma by Wael Abdullah Sultan Ali, Xinxin Huang, Yuehan Wu, Yuxiang Ma, Hui Pan, Jun Liao, Zhang Yang, Shaodong Hong, Yunpeng Yang, Yan Huang, Yuanyuan Zhao, Wenfeng Fang, Hongyun Zhao, and Li Zhang in Cancer Control.

Our study aimed to explore the prognostic value of the aspartate aminotransferase–platelet ratio index (APRI) and to develop a new nomogram for patients with hepatocellular carcinoma (HCC) who experience late recurrence after radiofrequency ablation (RFA). To date, no study has explored the value of APRI for assessing the late recurrence of HCC after RFA. The prognostic value of APRI was evaluated and validated in our multicenter retrospective analysis. A total of 466 HCC patients undergoing...

Our study aimed to explore the prognostic value of the aspartate aminotransferase–platelet ratio index (APRI) and to develop a new nomogram for patients with hepatocellular carcinoma (HCC) who experience late recurrence after radiofrequency ablation (RFA). To date, no study has explored the value of APRI for assessing the late recurrence of HCC after RFA. The prognostic value of APRI was evaluated and validated in our multicenter retrospective analysis. A total of 466 HCC patients undergoing...

Emerging zoonoses of wildlife origin caused by previously unknown agents are one of the most important challenges for human health. The Qinghai-Tibet Plateau represents a unique ecological niche with diverse wildlife that harbors several human pathogens and numerous previously uncharacterized pathogens. In this study, we identified and characterized a novel arenavirus (namely, plateau pika virus, PPV) from plateau pikas (Ochotona curzoniae) on the Qinghai-Tibet Plateau by virome analysis. Isolated PPV strains could replicate in several...

A dynamic treatment regime (DTR) is a sequence of decision rules that adapt to the time-varying states of an individual. Black-box learning methods have shown great potential in predicting the optimal treatments; however, the resulting DTRs lack interpretability, which is of paramount importance for medical experts to understand and implement. We present a stochastic tree-based reinforcement learning (ST-RL) method for estimating optimal DTRs in a multistage multitreatment setting with data from either randomized trials or...

Patients with depression have a high prevalence of developing dyslipidemia. In this study, we aim to investigate the difference of serum lipids, including total cholesterol (TCH), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), and triglycerides (TG), between the depressed patients and healthy controls. Sex differences in lipids and their psychological correlations were also included. The study included 56 healthy controls (males/females = 26/30) and 110 first-diagnosed drug-naïve outpatients (males/females = 35/75). A total of...

Numerous methods based on square rasters have been proposed for polygon generalization. However, these methods ignore the inconsistent distance measurement among neighborhoods of squares, which may result in an imbalanced generalization in different directions. As an alternative raster, a hexagon has consistent connectivity and isotropic neighborhoods. This study proposed a hexagon-based method for polygon generalization using morphological operators. First, we defined three generalization operators: aggregation, elimination, and line simplification, based on hexagonal morphological operations. We...

Additional file 4: Supplemental Table S4. Primers used for qRT- PCR

Additional file 2: Supplemental Table S2. ThesiRNA sequences of NC and siPCSK9

Additional file 3: Supplemental Table S3. Plasmid sequence of PCSK9

Additional file1: Fig. S1. OUM1 knockdown impairs UM cell metastasis and proliferation. Fig. S2. GO and pathway function analysis. Fig. S3. Bioinformatics analysis of OUM1 targets. Fig. S4. PTPRZ1 might be a target of OUM1. Fig. S5. Synergistic effect of SOV and OUM1 interference on apoptosis. Fig. S6. In vivo toxicity evaluation. Table S1. Downregulated and Upregulated gene. Table S2. Primers and siRNA used in this study. Table S3. Primers used in RNA-ChIP assay. Details...

A phytochemical investigation of Bidens procera L.C.Xu ex X.W.Zheng afforded two novel polyacetylenes, tridecane-2E-monoene-4,6,8-triyntylen-1,13-diol-12-O-β-glucoside (1) and tetradecane-2E,8E-diene-4,6-diyne-1,14-diol-13-O-β-glucoside (2), together with ten known compounds (3 − 12). Their chemical structures were elucidated by NMR and MS spectrums as well as the comparison of the published data. Furthermore, the chemotaxonomy of the yielded compounds was also discussed.

Supplemental Material for Pretreatment Serum Lactate Dehydrogenase and Metastases Numbers as Potential Determinants of Anti-PD-1 Therapy Outcome in Nasopharyngeal Carcinoma by Wael Abdullah Sultan Ali, Xinxin Huang, Yuehan Wu, Yuxiang Ma, Hui Pan, Jun Liao, Zhang Yang, Shaodong Hong, Yunpeng Yang, Yan Huang, Yuanyuan Zhao, Wenfeng Fang, Hongyun Zhao, and Li Zhang in Cancer Control.