745 Works

Smart phase transformation system based on lyotropic liquid crystalline@hard capsules for sustained release of hydrophilic and hydrophobic drugs

Xuejuan Zhang, Yujun Xiao, Zhengwei Huang, Jintian Chen, Yingtong Cui, Boyi Niu, Ying Huang, Xin Pan & Chuanbin Wu
Smart phase transformation systems@hard capsule (SPTS@hard capsule) based on lyotropic liquid crystalline (LLC) were developed for oral sustained release in this study. Doxycycline hydrochloride (DOXY) and meloxicam (MLX) were used as hydrophilic and hydrophobic model drug, respectively. Two systems were added with different additives, that is, gelucire 39/01, PEG 1000 and Tween 80 to adjust their melting point and release profiles. The phase transformation of these systems could be triggered by water as well as...

Isolation of Infectious SARS-CoV-2 from Urine of a COVID-19 Patient

Jing Sun, Airu Zhu, Heying Li, Kui Zheng, Zhen Zhuang, Zhao Chen, Yongxia Shi, Zhaoyong Zhang, Si-bei Chen, Xuesong Liu, Jun Dai, Xiaobo Li, Shuxiang Huang, Xiaofang Huang, Ling Luo, Liyan Wen, Jianfen Zhuo, Yuming Li, Yanqun Wang, Lu Zhang, Yanjun Zhang, Fang Li, Liqiang Feng, Xinwen Chen, Nanshan Zhong … & Yi-min Li
SARS-CoV-2 caused a major outbreak of severe pneumonia (COVID-19) in humans. Viral RNA was detected in multiple organs in COVID-19 patients. However, infectious SARS-CoV-2 was only isolated from respiratory specimens. Here, infectious SARS-CoV-2 was successfully isolated from urine of a COVID-19 patient. The virus isolated could infect new susceptible cells and was recognized by its’ own patient sera. Appropriate precautions should be taken to avoid transmission from urine.

Isolation of infectious SARS-CoV-2 from urine of a COVID-19 patient

Jing Sun, Airu Zhu, Heying Li, Kui Zheng, Zhen Zhuang, Zhao Chen, Yongxia Shi, Zhaoyong Zhang, Si-bei Chen, Xuesong Liu, Jun Dai, Xiaobo Li, Shuxiang Huang, Xiaofang Huang, Ling Luo, Liyan Wen, Jianfen Zhuo, Yuming Li, Yanqun Wang, Lu Zhang, Yanjun Zhang, Fang Li, Liqiang Feng, Xinwen Chen, Nanshan Zhong … & Yi-min Li
SARS-CoV-2 caused a major outbreak of severe pneumonia (COVID-19) in humans. Viral RNA was detected in multiple organs in COVID-19 patients. However, infectious SARS-CoV-2 was only isolated from respiratory specimens. Here, infectious SARS-CoV-2 was successfully isolated from urine of a COVID-19 patient. The virus isolated could infect new susceptible cells and was recognized by its’ own patient sera. Appropriate precautions should be taken to avoid transmission from urine.

Isolation of infectious SARS-CoV-2 from urine of a COVID-19 patient

Jing Sun, Airu Zhu, Heying Li, Kui Zheng, Zhen Zhuang, Zhao Chen, Yongxia Shi, Zhaoyong Zhang, Si-bei Chen, Xuesong Liu, Jun Dai, Xiaobo Li, Shuxiang Huang, Xiaofang Huang, Ling Luo, Liyan Wen, Jianfen Zhuo, Yuming Li, Yanqun Wang, Lu Zhang, Yanjun Zhang, Fang Li, Liqiang Feng, Xinwen Chen, Nanshan Zhong … & Yi-min Li
SARS-CoV-2 caused a major outbreak of severe pneumonia (COVID-19) in humans. Viral RNA was detected in multiple organs in COVID-19 patients. However, infectious SARS-CoV-2 was only isolated from respiratory specimens. Here, infectious SARS-CoV-2 was successfully isolated from urine of a COVID-19 patient. The virus isolated could infect new susceptible cells and was recognized by its’ own patient sera. Appropriate precautions should be taken to avoid transmission from urine.

Epidemiology and clinical course of COVID-19 in Shanghai, China

Yinzhong Shen, Fang Zheng, Danfeng Sun, Yun Ling, Jun Chen, Feng Li, Tao Li, Zhiping Qian, Yuyi Zhang, Qingnian Xu, Li Liu, Qin Huang, Fei Shan, Lie Xu, Jun Wu, Zhaoqin Zhu, Zhigang Song, Shenyang Li, Yuxin Shi, Jianliang Zhang, Xueyun Wu, Joshua B. Mendelsohn, Tongyu Zhu & Hongzhou Lu
Background: Novel coronavirus pneumonia (COVID-19) is prevalent around the world. We aimed to describe epidemiological features and clinical course in Shanghai. Methods: We retrospectively analysed 325 cases admitted at Shanghai Public Health Clinical Center, between January 20 and February 29, 2020. Results: 47.4% (154/325) had visited Wuhan within 2 weeks of illness onset. 57.2% occurred in 67 clusters; 40% were situated within 53 family clusters. 83.7% developed fever during the disease course. Median times from...

Inflectional paradigms as interacting systems

Eric R. Rosen

Post-translational knockdown and post-secretional modification of EsxA determine contribution of EsxA membrane permeabilizing activity for mycobacterial intracellular survival

Yanqing Bao, Lin Wang & Jianjun Sun
Current genetic studies (e.g. gene knockout) have suggested that EsxA and EsxB function as secreted virulence factors that are essential for Mycobaterium tuberculosis (Mtb) intracellular survival, specifically in mediating phagosome rupture and translocation of Mtb to the cytosol of host cells, which further facilitates Mtb intracellular replicating and cell-to-cell spreading. The EsxA-mediated intracellular survival is presumably achieved by its pH-dependent membrane-permeabilizing activity (MPA). However, the data from other studies have generated a discrepancy regarding the...

A 16S rRNA gene sequencing based study of oral microbiota in migraine patients in China

Weiqing Jiang, Tingting Wang, Chen Liu, Mingzhu Deng, Xiao Ren, Fei Wang, Yaqing Zhang, Xueying Yu, Lingling Yao & Yonggang Wang
Migraine is a primary headache characterized by moderate or severe headache attacks, accompanied with reversible neurological and systemic symptoms. There are rare biomarkers for the disease. While emerging evidence has indicated the connection between gut microbiota and migraine, the relation between oral microbiota and migraine is barely known. Thus, the objective of the current study was to explore a possible correlation between oral microbiota and migraine. We compared the oral microbiota communities of migraine patients...

Additional file 2 of Transcriptional dynamics of transposable elements when converting fibroblast cells of Macaca mulatta to neuroepithelial stem cells

Dahai Liu, Li Liu, Kui Duan, Junqiang Guo, Shipeng Li, Zhigang Zhao, Xiaotuo Zhang, Nan Zhou & Yun Zheng
Additional file 2 Supplementary Figure S1. The expression levels of LTRs in the Cluster L4 of Figure 3A. Supplementary Figure S2. The primers used to validate the expressions of 13 MacERV3 LTRs. (A) The alignment of the forward primer MacERV3_LTR2_34_F and the 13 MacERV3 LTRs. (B) The alignment of the reverse primer MacERV3_LTR2_34_R and the 13 MacERV3 LTRs. (C) The precise loci of the two primers in one of the selected MacERV3 LTRs, MacERV3_LTR2_34. Supplementary...

Alterations of oral microbiota in patients with panic disorder

Zunli Xie, Weiqing Jiang, Mingzhu Deng, Wei Wang, Xian Xie, Xia Feng, Yinping Shi, Xueyan Zhang, Dong Song, Ziyu Yuan & Yonggang Wang
The main characteristics of panic disorder (PD) include recurrent panic attacks and persistent worry, accompanied by other physical and cognitive symptoms. While recent studies have revealed that gut bacteria play an important role in anxiety and depression, little is known about the relationship between oral microbiota and PD. Therefore, the objective of this study was to explore a possible correlation between oral microbiota and PD. We conducted 16S rRNA sequencing to compare differences in the...

Aggregation-induced emission tetraphenylethylene derivative as optical sensor for ammonia detection

Shoubin Zhang, Di Zhao, Liuyi Xiong, Yufeng Hu, Zhidong Lou, Yanbing Hou, Feng Teng & Qiuhong Cui
We propose a general approach for the achievement of optical sensors, where ammonia can be rapidly identified and detected through visible fluorescence changes. Here, a tetraphenylethylene derivative with carboxyl groups named TPE-2CA (4,4ʹ-(1,2-Diphenylethene-1,2-diyl) dibenzoyl acid) was applied in the sensor. The AIE (aggregation-induced emission) property of tetraphenylethylene structure provides the samples with excellent luminescent performance in aggregated state. The reaction between carboxyl groups and ammonia causes the shifts on luminescence spectra of the samples. Consequently,...

Reliability and validity of the Korean version of the Functional Status Score for the ICU after translation and cross-cultural adaptation

Jong Geol, Gee Young Suh, Yu Hui Won, Won Hyuk Chang, Stephanie Hiser, Dale M. Needham & Chi Ryang Chung
The Functional Status Score for the Intensive Care Unit (FSS-ICU) evaluates the physical function of ICU patients. The objective of the study was to translate and cross-culturally adapt the FSS-ICU into Korean and assess its reliability and validity. An expert committee supervised the forward and backward translation process and the final translated version. Experienced physiotherapists assessed patients (n= 31) from a medical ICU using the Korean version of FSS-ICU. Intra-class correlation coefficients (ICCs) and Bland–Altman’s...

BUB1B (BUB1 Mitotic Checkpoint Serine/Threonine Kinase B) promotes lung adenocarcinoma by interacting with Zinc Finger Protein ZNF143 and regulating glycolysis

Xiaolei Zhou, Yanli Yuan, Hongping Kuang, Bingxiang Tang, Hui Zhang & Manlin Zhang
Lung adenocarcinoma (LUAD) is one of the most common causes of cancer death in men. BUB1B (BUB1 mitotic checkpoint serine/threonine kinase B) has been reported to contribute to the initiation and development of several cancers. Here, we aimed to explore the potential role of BUB1B in LUAD. We found BUB1B was upregulated in LUAD, suggesting its potential role as a biomarker for LUAD diagnosis. Significantly, LUAD patients with high BUB1B expression had a shorter survival...

Stakeholders’ Perceptions of a School-Based Eye Care Programme in Baltimore, MD

Alyssa M. Kretz, Hursuong Vongsachang, David S. Friedman, Jonathan Callan, Madison Wahl, M. Rani Mukherjee, Amanda Neitzel & Megan E. Collins
To explore stakeholders’ perceptions of a school-based vision programme (SBVP). We conducted 20 focus groups with 105 parents and teachers at schools in Baltimore, MD, that participated in a SBVP. Facilitators used a semi-structured interview guide to discuss participants’ perceptions of the SBVP. Focus groups were audio-recorded, transcribed, and coded using inductive thematic analysis. Participant perceptions fell into three categories: benefits of school-based eye care, limitations of school-based eye care, and observation of impact. The...

Acetylation of SCFD1 regulates SNARE complex formation and autophagosome-lysosome fusion

Hong Huang, Qinqin Ouyang, Kunrong Mei, Ting Liu, Qiming Sun, Wei Liu & Rong Liu
SCFD1 (sec1 family domain containing 1) was recently shown to function in autophagosome-lysosome fusion, and multiple studies have demonstrated the regulatory impacts of acetylation (a post-translational modification) on macroautophagy/autophagy. Here, we demonstrate that both acetylation- and phosphorylation-dependent mechanisms control SCFD1ʹs function in autophagosome-lysosome fusion. After detecting a decrease in the extent of SCFD1 acetylation under autophagy-stimulated conditions, we found that KAT2B/PCAF catalyzes the acetylation of residues K126 and K515 of SCFD1; we also showed that...

M. tuberculosis CRISPR/Cas proteins are secreted virulence factors that trigger cellular immune responses

Jianjian Jiao, Nan Zheng, Wenjing Wei, Joy Fleming, Xingyun Wang, Zihui Li, Lili Zhang, Yi Liu, Zongde Zhang, Adong Shen, Li Chuanyou, Lijun Bi & Hongtai Zhang
The role of prokaryotic CRISPR/Cas system proteins as a defensive shield against invasive nucleic acids has been studied extensively. Non-canonical roles in pathogenesis involving intracellular targeting of certain virulence-associated endogenous mRNA have also been reported for some Type I and Type II CRISPR/Cas proteins, but no such roles have yet been established for Type III system proteins. Here, we demonstrate that M. tuberculosis (Type III-A system) CRISPR/Cas proteins Csm1, Csm3, Csm5, Csm6, and Cas6 are...

Additional file 3 of Mucosal-associated invariant T cells predict increased acute graft-versus-host-disease incidence in patients receiving allogeneic hematopoietic stem cell transplantation

Zhao Wang, Sudong Zhang, Xiaoyu Zhang, Li Liu, Lukun Zhou, Yuyan Shen, Rongli Zhang, Yi He, Donglin Yang, Erlie Jiang, Xiaoming Feng, Jiaxi Zhou, Tao Cheng, Mingzhe Han & Sizhou Feng
Additional file 3: Hub genes of each enriched gene set.

Additional file 3 of Mucosal-associated invariant T cells predict increased acute graft-versus-host-disease incidence in patients receiving allogeneic hematopoietic stem cell transplantation

Zhao Wang, Sudong Zhang, Xiaoyu Zhang, Li Liu, Lukun Zhou, Yuyan Shen, Rongli Zhang, Yi He, Donglin Yang, Erlie Jiang, Xiaoming Feng, Jiaxi Zhou, Tao Cheng, Mingzhe Han & Sizhou Feng
Additional file 3: Hub genes of each enriched gene set.

sj-docx-1-tct-10.1177_15330338221132035 - Supplemental material for Construction and Validation of Prognosis Nomogram for Metastatic Lung Squamous Cell Carcinoma: A Population-Based Study

Yuting Liu, Min Sun, Ying Xiong, Xinyue Gu, Kai Zhang & Li Liu
Supplemental material, sj-docx-1-tct-10.1177_15330338221132035 for Construction and Validation of Prognosis Nomogram for Metastatic Lung Squamous Cell Carcinoma: A Population-Based Study by Yuting Liu, Min Sun, Ying Xiong, Xinyue Gu, Kai Zhang and Li Liu in Technology in Cancer Research & Treatment

Additional file 1 of A systematic review of cost-utility analyses of screening methods in latent tuberculosis infection in high-risk populations

James Mahon, Sophie Beale, Hayden Holmes, Mick Arber, Vladyslav Nikolayevskyy, Riccardo Alagna, Davide Manissero, David Dowdy, Giovanni Battista Migliori, Giovanni Sotgiu & Raquel Duarte
Additional file 1. Search strategy.

sj-docx-1-jiv-10.1177_08862605221124263 – Supplemental material for Child Sexual Abuse in the Roman Catholic Church in France: Prevalence and Comparison With Other Social Spheres

Nathalie Bajos, Julie Ancian, Josselin Tricou, Axelle Valendru, Jeanna-Eve Pousson & Caroline Moreau
Supplemental material, sj-docx-1-jiv-10.1177_08862605221124263 for Child Sexual Abuse in the Roman Catholic Church in France: Prevalence and Comparison With Other Social Spheres by Nathalie Bajos, Julie Ancian, Josselin Tricou, Axelle Valendru, Jeanna-Eve Pousson and Caroline Moreau in Journal of Interpersonal Violence

Additional file 3 of The dynamic changes and sex differences of 147 immune-related proteins during acute COVID-19 in 580 individuals

Guillaume Butler-Laporte, Edgar Gonzalez-Kozlova, Chen-Yang Su, Sirui Zhou, Tomoko Nakanishi, Elsa Brunet-Ratnasingham, David Morrison, Laetitia Laurent, Jonathan Afilalo, Marc Afilalo, Danielle Henry, Yiheng Chen, Julia Carrasco-Zanini, Yossi Farjoun, Maik Pietzner, Nofar Kimchi, Zaman Afrasiabi, Nardin Rezk, Meriem Bouab, Louis Petitjean, Charlotte Guzman, Xiaoqing Xue, Chris Tselios, Branka Vulesevic, Olumide Adeleye … & J Brent Richards
Additional file 3: Protein correlation heatmaps.

Additional file 4 of The dynamic changes and sex differences of 147 immune-related proteins during acute COVID-19 in 580 individuals

Guillaume Butler-Laporte, Edgar Gonzalez-Kozlova, Chen-Yang Su, Sirui Zhou, Tomoko Nakanishi, Elsa Brunet-Ratnasingham, David Morrison, Laetitia Laurent, Jonathan Afilalo, Marc Afilalo, Danielle Henry, Yiheng Chen, Julia Carrasco-Zanini, Yossi Farjoun, Maik Pietzner, Nofar Kimchi, Zaman Afrasiabi, Nardin Rezk, Meriem Bouab, Louis Petitjean, Charlotte Guzman, Xiaoqing Xue, Chris Tselios, Branka Vulesevic, Olumide Adeleye … & J Brent Richards
Additional file 4: Protein clusters.

Additional file 5 of The dynamic changes and sex differences of 147 immune-related proteins during acute COVID-19 in 580 individuals

Guillaume Butler-Laporte, Edgar Gonzalez-Kozlova, Chen-Yang Su, Sirui Zhou, Tomoko Nakanishi, Elsa Brunet-Ratnasingham, David Morrison, Laetitia Laurent, Jonathan Afilalo, Marc Afilalo, Danielle Henry, Yiheng Chen, Julia Carrasco-Zanini, Yossi Farjoun, Maik Pietzner, Nofar Kimchi, Zaman Afrasiabi, Nardin Rezk, Meriem Bouab, Louis Petitjean, Charlotte Guzman, Xiaoqing Xue, Chris Tselios, Branka Vulesevic, Olumide Adeleye … & J Brent Richards
Additional file 5: Inferred protein levels over time.

Additional file 1 of The dynamic changes and sex differences of 147 immune-related proteins during acute COVID-19 in 580 individuals

Guillaume Butler-Laporte, Edgar Gonzalez-Kozlova, Chen-Yang Su, Sirui Zhou, Tomoko Nakanishi, Elsa Brunet-Ratnasingham, David Morrison, Laetitia Laurent, Jonathan Afilalo, Marc Afilalo, Danielle Henry, Yiheng Chen, Julia Carrasco-Zanini, Yossi Farjoun, Maik Pietzner, Nofar Kimchi, Zaman Afrasiabi, Nardin Rezk, Meriem Bouab, Louis Petitjean, Charlotte Guzman, Xiaoqing Xue, Chris Tselios, Branka Vulesevic, Olumide Adeleye … & J Brent Richards
Additional file 1: List of immunity-related proteins measured.

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Affiliations

  • Johns Hopkins University
    745
  • Chinese Academy of Medical Sciences & Peking Union Medical College
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    311
  • Huazhong University of Science and Technology
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