This study aimed to clone the full-length open reading frame (ORF) of goat ANGPTL8 gene sequence, reveal its molecular and expression characteristics, and explore its effect on the differentiation of goat intramuscular preadipocytes. The full-length ORF sequence of goat ANGPTL8 gene was cloned by RT-PCR technology, and bioinformatics analysis was performed by related biological software. RT-qPCR was used to detect the expression of ANGPTL8 mRNA in goat tissues. Further use of RNA interference to study...

The intracellular pathogen Salmonella enterica serovar Typhimurium (S. Typhimurium) exploits host macrophage as a crucial survival and replicative niche. To minimize host immune response stimulated by flagellin, the expression of flagellar genes is downregulated during S. Typhimurium growth within host macrophages. However, the underlying mechanisms are largely unknown. In this study, we show that STM14_1285 (named AsiR), a putative RpiR-family transcriptional regulator, which is downregulated within macrophages as previously reported and also confirmed here, positively...

Preeclampsia (PE) is a disorder that occurs during the pregnancy and could affect the maternal and perinatal mortality as well as morbidity. The aim of our study is to investigate the associations between the hypertension susceptibility genes ITGA9, MOV10 and CACNB2 with PE in Chinese Han population. A case–control study including 178 PE patients and 202 healthy controls was conducted to assess the associations between three loci (ITGA9 rs155524, MOV10 rs2932538 and CACNB2 rs4373814) and...

To compare perioperative outcomes and long-term renal function changes between prior stenting (PS) and not prior stenting (NPS) before flexible ureteroscopy lithotripsy (f-URS) for solitary kidney patients. Solitary kidney patients with 10–30 mm renal stones were enrolled in this historical control study. Perioperative parameters and complications were compared. Stone-free was defined as the absence of any residual stones on a CT scan. Renal function changes were evaluated by estimated glomerular filtration rate (eGFR) and adjusted...

We studied the boundary layer effects on flame temperature measurements using line-of-sight (LOS) absorption spectroscopy. The primary objective is to quantify the measurement uncertainty of the LOS-determined temperature, which is influenced by the thermal and species boundary layers existing in standard laminar premixed flames. The boundary layer thickness (δ), central flame temperature (Tc) and species concentration (Xc) are the major factors investigated in this work. Typical absorption lines of H2O in the wavelength range of...

The functions of a large number of genes, including gene families with domains of unknown functions (DUF), still remain unclear. In this study, we analyzed four members of OsDUF1664 (OsDUF1664.1-OsDUF1664.4) in rice Nipponbare. By phylogenetic analysis, DUF1664 members in rice and Arabidopsis were classified into three major groups (I, II, III). Under drought conditions, the expression level of OsDUF1664.3 in rice was significantly elevated. Overexpression of OsDUF1664.3 in Escherichia coli led to a significant enhancement...

Supplemental Material, sj-pdf-1-jfi-10.1177_0192513X211042843 for Interparental Conflict and Chinese Children’s Social Development by Fengjiao Xu, Xinyi Chen, Haiyan Xing and Hongmei Wang in Journal of Family Issues

Supplemental Material, sj-pdf-1-ssc-10.1177_08944393211042575 for Strategic Framing Matters But Varies: A Structural Topic Modeling Approach to Analyzing China’s Foreign Propaganda About the 2019 Hong Kong Protests on Twitter by Maggie Mengqing Zhang, Xiao Wang and Yang Hu in Social Science Computer Review

A new type of metal-free catalyst was successfully prepared by doping boron (B) in the carbon nanotube. The catalyst had 99.4% removal of phenol in 60 min at pH 7 by activating peroxymonosulfate (PMS). In order to explore the origin of the high catalytic activity, the samples were characterized by Raman and electron paramagnetic resonance (EPR), and the reactive oxygen species (ROS) in the process of catalytic degradation were investigated. The Raman results showed that...

As a form of pollution source control and a low-impact development measure, bioretention is a convenient, economical, and effective method for the removal of heavy metals from stormwater runoff, which can adapt to the randomness and uncontrollability of non-point source pollution. However, few studies have assessed the performance of bioretention in the simultaneous removal of multiple heavy metals and the impact of heavy metal migration on the bioretention life cycle. In this study, the removal...

Acute kidney injury (AKI) is one of the most common complications after cardiac surgery. However, effective biomarker used for early diagnosis of AKI has not been identified. Platelet-leukocyte aggregates (PLAs) participate in inflammation and coagulation, leading to vascular lesions and tissue destruction. We designed a prospective study to assess whether PLAs can serve as a good biomarker for early diagnosis of AKI after cardiac surgery. Patients with rheumatic heart disease scheduled to undergo valve replacement...

In this study, we prepared a folic acid-functionalized SMMC-7721 liver cancer cell membrane (CM)-encapsulated paclitaxel nanocrystals system (FCPN) for hepatoma treatment. Transmission electron microscopy (TEM) characterization showed that FCPN was irregular spherical shapes with a particle size larger than 200 nm and a coated thickness of approximately 20 nm. In an in vitro release experiment, FCPN indicated a slowly release effect of paclitaxel (PTX). Cell experiments demonstrated that FCPN was taken up by SMMC-7721 cells...

A new type of metal-free catalyst was successfully prepared by doping boron (B) in the carbon nanotube. The catalyst had 99.4% removal of phenol in 60 min at pH 7 by activating peroxymonosulfate (PMS). In order to explore the origin of the high catalytic activity, the samples were characterized by Raman and electron paramagnetic resonance (EPR), and the reactive oxygen species (ROS) in the process of catalytic degradation were investigated. The Raman results showed that...

The Wanbaogou granitic plutons are exposed along with coeval volcanic rocks, and provide an opportunity to investigate Silurian tectonism in the South Kunlun belt, northwest China. This study presents new whole-rock geochemical, and zircon U–Pb geochronological and Lu–Hf isotope data for Silurian intrusive rocks of the Wanbaogou area. We analysed four samples: a porphyritic monzogranite and dioritic dike from the Wanbaogou pluton and a granodiorite and monzogranite from the Wudaogou pluton. Zircon U–Pb dating yielded...

A new cyclopentenone derivative, atrovinol (1), together with ten known compounds (2-11) were isolated from Trichoderma atroviride HH-01, an endophytic fungus from Illigera rhodantha (Hernandiaceae). Their structures were identified by HRESIMS, 1 D/2D NMR, and electronic circular dichroism (ECD) spectra. Compound 1 exhibited moderate inhibitory activity against Staphylococcus aureus and Bacillus subtilis with MIC values of 8.0 µg/mL and 16.0 µg/mL, respectively.

In order to explore new prediction methods and key genes for gastric cancer. Firstly, we downloaded the 6 original sequencing data of gastric cancer on the Illumina HumanHT-12 platform from Array Expression and Gene Expression Omnibus, and used bioinformatics methods to identify 109 up-regulated genes and 271 down-regulated genes. Further, we performed univariate Cox regression analysis of prognostic-related genes, then used Lasso regression to remove collinearity, and finally used multivariate Cox regression to analyze independent...

In order to explore new prediction methods and key genes for gastric cancer. Firstly, we downloaded the 6 original sequencing data of gastric cancer on the Illumina HumanHT-12 platform from Array Expression and Gene Expression Omnibus, and used bioinformatics methods to identify 109 up-regulated genes and 271 down-regulated genes. Further, we performed univariate Cox regression analysis of prognostic-related genes, then used Lasso regression to remove collinearity, and finally used multivariate Cox regression to analyze independent...

For revealing molecular markers related to the traits of multiple lumbar vertebrae in sheep, we analyze the relationship between NR6A1 gene polymorphism and lumbar vertebrae number traits in Xinjiang Kazakh sheep. Lumbar muscle tissues were collected from 6-lumbar spine (L6) Kazak sheep and 7-lumbar spine (L7) Kazak sheep and the intron-8 of NR6A1 gene was amplified by PCR. The SNP locus was detected by the PCR-SSCP method. One-Way ANOVA and an Independent Chi-square Test is...

Natural plant-derived protein with excellent bioactivities has attracted much attention so a functional protein with molecular weight of 15.2 kDa was extracted from Millettia speciosa Champ. leaf for the first time. Under the pH of 12.0, solid-liquid ratio of 1:40 (w/v), extraction time of 2.0 h, and extraction temperature of 50 °C, the highest extracting efficiency (79.25 ± 0.78%) of the Millettia speciosa Champ. leaf protein (MLP) was achieved. The main structure of MLP contained...

The rapid growth of global energy demand necessitates high-performance water-based drilling fluids (WDFs) with excellent-plugging performance in the deep excavation of shale gas formation. Herein, we report an organic-inorganic nanocomposite (NS-D) as a plugging agent in WDFs to plug the nanoporous of shale and abate the hydration expansion of shale by integrating the advantages of inorganic and polymer nano plugging agents. The results of Fourier Transform infrared spectroscopy (FTIR) and proton nuclear magnetic resonance (H-NMR)...

Recovering phosphorus (P) from excess sludge of wastewater treatment plants (WWTPs) has attracted considerable attention. An efficient P release method is undoubtedly critical for a satisfactory recovery performance. In this study, the effectiveness of three sludge pretreatment methods, i.e. anaerobic digestion (AD), EDTA-anaerobic digestion (EA) and ultrasound combined with EA (U-EA), on P release and struvite recovery from excess sludge was investigated. The results showed that different pretreatment methods resulted in the different characteristics of...

Recovering phosphorus (P) from excess sludge of wastewater treatment plants (WWTPs) has attracted considerable attention. An efficient P release method is undoubtedly critical for a satisfactory recovery performance. In this study, the effectiveness of three sludge pretreatment methods, i.e. anaerobic digestion (AD), EDTA-anaerobic digestion (EA) and ultrasound combined with EA (U-EA), on P release and struvite recovery from excess sludge was investigated. The results showed that different pretreatment methods resulted in the different characteristics of...

A growing body of evidence suggests the pivotal role of long non-coding RNA (lncRNA) in influenza virus infection. Based on next-generation sequencing, we previously demonstrated that Lnc45 was distinctively stimulated by H5N1 influenza virus in mice. In this study, we systematically investigated the specific role of Lnc45 during influenza A virus (IAV) infection. Through qRT-PCR, we first demonstrated that Lnc45 is highly up-regulated by different subtypes of IAV strains, including H5N1, H7N9, and H1N1 viruses....

Long non-coding RNAs (lncRNAs) play important regulatory roles in hepatocellular carcinoma (HCC). However, the function of LOC107985656 in HCC progression remains unclear. The lncRNA, mRNA and miRNA levels in HCC tissues or cells were measured using real-time quantitative polymerase chain reaction (RT-qPCR). The proliferation of cancer cells was evaluated using 3-(4, 5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) viability and colony formation assays. Bioinformatics prediction, dual luciferase assay and RNA pull-down assay were performed to analyze the relationships between...

Cisplatin (DDP) is a first-line chemotherapeutic drug applied for the treatment of oral squamous cell carcinoma (OSCC). The anticancer activity of DDP is tightly linked to its intracellular uptake. It is unwise to increase the DDP intake by increasing the dose or shortening the dosing interval because of the severe systemic toxicity (nephrotoxicity, ototoxicity and neurotoxicity) in DDP application. The main uptake pathways of DDP include passive diffusion and active transporter transport. Therefore, finding additional...