3,087 Works

Second generation β-elemene nitric oxide derivatives with reasonable linkers: potential hybrids against malignant brain glioma

Renren Bai, Junlong Zhu, Ziqiang Bai, Qing Mao, Yingqian Zhang, Zi Hui, Xinyu Luo, Xiang-Yang Ye & Tian Xie
Elemene is a second-line broad-spectrum anti-tumour drug that has been used in China for more than two decades. However, its main anti-tumour ingredient, β-elemene, has disadvantages, including excessive lipophilicity and relatively weak anti-tumour efficacy. To improve the anti-tumour activity of β-elemene, based on its minor molecular weight character, we introduced furoxan nitric oxide (NO) donors into the β-elemene structure and designed six series of new generation β-elemene NO donor hybrids. The synthesised compounds could effectively...

Network pharmacology and in vitro experimental verification to explore the mechanism of Sanhua decoction in the treatment of ischaemic stroke

Wei Zhang, Li Zhang, Wen jun Wang, Shanbo Ma, Mingming Wang, Minna Yao, Ruili Li, Wei wei Li, Xian Zhao, Dongmei Hu, Yi Ding & Jingwen Wang
Stroke is an illness with high morbidity, disability and mortality that presents a major clinical challenge. Sanhua decoction (SHD) has been widely used to treat ischaemic stroke in the clinic. However, the potential mechanism of SHD remains unknown. To elucidate the multitarget mechanism of SHD in ischaemic stroke through network pharmacology and bioinformatics analyses. Network pharmacology and experimental validation approach was used to investigate the bioactive ingredients, critical targets and potential mechanisms of SHD against...

Kavalactones isolated from Alpinia zerumbet (Pers.) Burtt. et Smith with protective effects against human umbilical vein endothelial cell damage induced by high glucose

Hualin You, Min He, Di Pan, Guanqin Fang, Yan Chen, Xu Zhang, Xiangchun Shen & Nenling Zhang
A new kavalactone, 4'-hydroxyl dihydro-5, 6-dehydrokavain (1) was isolated from the petroleum ether partition of leaves of Alpinia zerumbet (Pers.) Burtt. et Smith, together with four known kavalactone dimers, rel-1,trans-3-bis-(4-methoxy-2-oxopyran-6-yl)-cis-2,trans-4-diphenyl cyclobutene (2), aniba dimer A (3), aniba dimer C (4), 6,6'-(3,4-diphenylcyclobutane-1,2-diyl)bis(4-methoxy-2H-pyran-2-one (5). The structure of compound 1 was characterized by its MS, 1D-NMR, and 2D-NMR data, and the structures of the known compounds were determined by comparison of their spectroscopic data with those reported by...

Network pharmacology and in vitro experimental verification to explore the mechanism of Sanhua decoction in the treatment of ischaemic stroke

Wei Zhang, Li Zhang, Wen jun Wang, Shanbo Ma, Mingming Wang, Minna Yao, Ruili Li, Wei wei Li, Xian Zhao, Dongmei Hu, Yi Ding & Jingwen Wang
Stroke is an illness with high morbidity, disability and mortality that presents a major clinical challenge. Sanhua decoction (SHD) has been widely used to treat ischaemic stroke in the clinic. However, the potential mechanism of SHD remains unknown. To elucidate the multitarget mechanism of SHD in ischaemic stroke through network pharmacology and bioinformatics analyses. Network pharmacology and experimental validation approach was used to investigate the bioactive ingredients, critical targets and potential mechanisms of SHD against...

DNA topoisomerase II alpha promotes the metastatic characteristics of glioma cells by transcriptionally activating β-catenin

Yi Liu, Jun Ma, Jiu-Shan Song, Hai-Ying Zhou, Jing-Hui Li, Cheng Luo, Xin Geng & He-Xiang Zhao
DNA topoisomerase II alpha (TOP2A) reportedly plays a crucial role in several cancers, however, the precise regulatory role of TOP2A in metastatic characteristics of glioma is still poorly understood. Herein, we sought to elucidate the mechanisms by which TOP2A affects the metastatic phenotypes of glioma. We observed that a high level of TOP2A expression was dramatically linked with inferior survival in glioma patients while silencing of TOP2A impaired glioma cell proliferation and aggressiveness. TOP2A was...

DNA topoisomerase II alpha promotes the metastatic characteristics of glioma cells by transcriptionally activating β-catenin

Yi Liu, Jun Ma, Jiu-Shan Song, Hai-Ying Zhou, Jing-Hui Li, Cheng Luo, Xin Geng & He-Xiang Zhao
DNA topoisomerase II alpha (TOP2A) reportedly plays a crucial role in several cancers, however, the precise regulatory role of TOP2A in metastatic characteristics of glioma is still poorly understood. Herein, we sought to elucidate the mechanisms by which TOP2A affects the metastatic phenotypes of glioma. We observed that a high level of TOP2A expression was dramatically linked with inferior survival in glioma patients while silencing of TOP2A impaired glioma cell proliferation and aggressiveness. TOP2A was...

One novel naphthalene derivative and other constituents with anti-complementary activities from the aerial parts of Dracocephalum moldavica

Jia-Ming Wang, Jin-Feng Sun, Long Jin, Meng-Jie Wang, Yan-Yan Huang, Mei Jin, Wei Zhou & Gao Li
One novel naphthalene derivative, 2-octa-2′,4′,6′-atriynenaphthalene (1), together with eighteen known compounds (2–19) were isolated from the aerial parts of Dracocephalum moldavica L. Compounds 2, 8, 10, 13, 15–17 and 19 were obtained from the family Lamiaceae for the first time, and compounds 11 and 18 were firstly identified from the genus of Dracocephalum. All the isolates were evaluated for anti-complementary activities through the classical and alternative pathways, and the targets of the most active compounds...

Circ_0017639 facilitates proliferative, migratory, and invasive potential of non-small cell lung cancer (NSCLC) cells via PI3K/AKT signaling pathway

Hong-Bo Zhang, Xiang-Ming Qiu, Yi-Chao Zhang, Ting-Ting Huang, Zhan-Jie Zuo & Tao Zhang
Non-small cell lung cancer (NSCLC) has increased morbidity and mortality rate worldwide. The current NSCLS therapies are associated with poor outcomes and need further improvement. CircRNAs were shown to regulate NSCLC progression. However, little is known re garding the functions and mechanisms of circ_0017639 in NSCLC, which requires further extensive studies. The circ_0017639 expression in NSCLC tissues and cell lines was evaluated via qRT-RCR. Moreover, using ectopic plasmid incorporation and shRNA assays, we analyzed the...

Risk factors influencing cure of ultrasound-guided microwave ablation for primary hyperparathyroidism

Fangyi Liu, Li Zang, Yang Liu, Xiaoling Yu, Zhigang Cheng, Zhiyu Han, Jie Yu & Ping Liang
To evaluate the potential risk factors influencing cure rate of ultrasound-guided microwave ablation (MWA) for primary hyperparathyroidism (PHPT). Seventy five patients (25 males and 50 females; mean age, 56.80 ± 12.34; age range, 26–85) with PHPT undergoing MWA under ultrasound guidance were enrolled between May 2017 and December 2020. The cure rate and complications were evaluated after treatment. The potential factors influencing cure rate of ultrasound-guided MWA for PHPT were analyzed by univariate and multivariate...

Bioactive polyacetylenes from Bidens pilosa L and their anti-inflammatory activity

Zhi Yan, Zhenshan Chen, Ling Zhang, Xiaoming Wang, Yaowen Zhang & Zhenhua Tian
A new polyacetylenic glucoside together with three known compounds were isolated from the whole herb of Bidens pilosa L. The structure elucidation of these four compounds was employed by combining NMR and HR-MS data as 4-O-β-D-glucopyranosyloxy-1-hydroxy- 6-(E)-tetradecene-8,10,12-triyne (1), 3-O-β-D-glucopyranosyloxy-1-hydroxy-6-(E)-tetradec- ene-8,10,12-triyne (2), 2-O-β-D-glucopyranosyloxy-1-hydroxy-5-(E)-tetradecene-7,9,11-triy- ne (3) and icthyothereol acetate (4). Additionally, bioactivity study showed that these compounds have potential anti-inflammatory activity. This study has certain guiding significance for the development and utilisation of polyacetylene compounds in Bidens...

The Effect of the Acidic Gas Pollutant on Wool Keratin Fiber Structure

Zhe Jiang, Rubing Bai, Jinfang Zhang, Ende Li, Yifan Cui, Ning Zhang, Nan Zhang & Qiang Wang
Increase in air pollution caused by acidic gases, such as nitrogen dioxide, call for the need to study potential effects on the structure of textile materials. Wool is a natural protein fiber which is easily damaged due to rich reaction positions and cross-linked bonds in the fiber. This study reports, for the first time, effect of nitrogen dioxide, as a model acid gas pollutant, on the structure of wool. On the macro level, wool fabric...

Nucleosides and amino acids, isolated from Cordyceps sinensis, protected against cyclophosphamide-induced myelosuppression in mice

Yu Zhang, Jie Liu, Yan Wang, Chengpeng Sun, Wenjia Li, Jianjian Qiu, Yanling Qiao, Fan Wu, Xiaokui Huo, Yue An, Baojing Zhang, Shuangcheng Ma, Jian Zheng & Xiaochi Ma
The material basis of Cordyceps sinensis (Berk.) Sacc has not yet been well understood and natural C. sinensis resources are very rare. The present study aimed to clarify the substance basis and compare the protective effect of natural and artificially-cultivated C. sinensis against cyclophosphamide (CTX)-induced myelosuppression. Both natural and artificially-cultivated C. sinensis effectively improved CTX-induced decrease of peripheral blood counts and hemopoietic growth factors, pathological changes, and apoptosis of bone marrow. Importantly, artificially-cultivated C. sinensis...

Defective autophagy contributes to endometrial epithelial-mesenchymal transition in intrauterine adhesions

Zhenhua Zhou, Huiyan Wang, Xiwen Zhang, Minmin Song, Simin Yao, Peipei Jiang, Dan Liu, Zhiyin Wang, Haining Lv, Ruotian Li, Ying Hong, Jianwu Dai, Yali Hu & Guangfeng Zhao
Intrauterine adhesions (IUA), characterized by endometrial fibrosis, is a common cause of uterine infertility. We previously demonstrated that partial epithelial-mesenchymal transition (EMT) and the loss of epithelial homeostasis play a vital role in the development of endometrial fibrosis. As a pro-survival strategy in maintaining cell and tissue homeostasis, macroautophagy/autophagy, conversely, may participate in this process. However, the role of autophagy in endometrial fibrosis remains unknown. Here, we demonstrated that autophagy is defective in endometria of...

The association between histological prostatitis and benign prostatic hyperplasia: a single-center retrospective study

Jinze Li, Yunxiang Li, Dehong Cao, Yin Huang, Lei Peng, Chunyang Meng & Qiang Wei
To investigate the relationship between histological prostatitis (HP) and clinical parameters related to benign prostatic hyperplasia (BPH) in male. The clinical data of 196 BPH patients who underwent transurethral resection of the prostate were collected. According to the results of hematoxylin-eosin (H&E) staining of prostate tissue, patients were divided into two groups: BPH with HP group and BPH without HP group. Differences in acute urinary retention (AUR), prostate volume (PV), serum sex hormones, lower urinary...

A novel big data mining framework for reconstructing large-scale daily MAIAC AOD data across China from 2000 to 2020

Binjie Chen, Yang Ye, Cheng Tong, Jinsong Deng, Ke Wang & Yang Hong
Satellite-based aerosol optical depth (AOD) retrieval products are essential in air pollution and climate change research. Unfortunately, cloud contaminations and unfavorable surface conditions result in a considerable proportion of missing AOD data. Numerous studies have been conducted to reconstruct large-scale AOD data gaps by utilizing adjacent spatiotemporal information or modeling AOD data via various external geographical data. However, the erratic variation of AOD and the inconvenience of external data weaken the accuracy and efficiency of...

Reproducibility of the Infinium methylationEPIC BeadChip assay using low DNA amounts

Steffan Noe Christiansen, Jeppe Dyrberg Andersen, Marie-Louise Kampmann, Jing Liu, Mikkel Meyer Andersen, Jacob Tfelt-Hansen & Niels Morling
The Infinium MethylationEPIC BeadChip (EPIC) is a reliable method for measuring the DNA methylation of more than 850,000 CpG positions. In clinical and forensic settings, it is critical to be able to work with low DNA amounts without risking reduced reproducibility. We evaluated the EPIC for a range of DNA amounts using two-fold serial dilutions investigated on two different days. While the β-value distributions were generally unaffected by decreasing DNA amounts, the median squared Pearson’s...

Primary graft failure following allogeneic hematopoietic stem cell transplantation: risk factors, treatment and outcomes

Juan Chen, Aiming Pang, Yuanqi Zhao, Li Liu, Runzhi Ma, Jialin Wei, Xin Chen, Yi He, Donglin Yang, Rongli Zhang, Weihua Zhai, Qiaoling Ma, Erlie Jiang, Mingzhe Han, Jiaxi Zhou & Sizhou Feng
Graft failure (GF) is an intractable complication of transplantation, which can severely affect the efficacy of the graft; however, the characteristics, incidence, and risk factors of primary GF have not been well described. This study aimed to analyze the risk factors and outcomes of primary GF to swiftly identify high-risk patients for GF. We performed a case-control study with a case-control ratio of 1:4 with 869 patients who underwent allogeneic hematopoietic stem cell transplantation (allo-HSCT)...

AFAP1 antisense RNA 1 promotes retinoblastoma progression by sponging microRNA miR-545-3p that targets G protein subunit beta 1

Wenting Tang, Li Zhang, Jing Li & Yu Guan
The oncogenic role of actin filament-associated protein 1 antisense RNA 1 (AFAP1-AS1) has been reported in retinoblastoma (RB). However, the underlying regulatory mechanisms remain poorly understood. In this study, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and Western blotting were performed to analyze the expression of AFAP1-AS1, microRNA miR-545-3p, or G protein subunit beta 1 (GNB1). Cell Counting Kit-8 (CCK-8) and Transwell migration assays were used to detect cell proliferation and migration. In addition, caspase-3 activity...

Water-soluble chitosan and phytoremediation efficiency of two Brassica napus L. cultivars in cadmium-contaminated farmland soils

Jian-Lin Bian, Wei Cao, Jun-Mei Guo, Jun-Xing Yang, Xue-Dong Wang, Jie Wang, Jun Huang, Tian-Xiang Xia & Cun-Yan Xia
Pot and field trials were conducted to investigate Cd uptake and phytoremediation efficiency of two Brassica napus cultivars (QY-1 and SYH) with applied water-soluble chitosan (WSC, Pot: 0, 2% and 4%; Field: 0 and 10 g·m−2) grown in Cd-contaminated soils. The results from the pot and field trials generally showed that WSC treatments significantly increased Cd concentrations in shoot and root tissues by 33.77–159.71% (except for SYH/JY) and 7.42–168.71% of two B. napus cultivars compared...

The role of gut microbiota in patients with benign and malignant brain tumors: a pilot study

Haixiao Jiang, Wei Zeng, Xiaoli Zhang, Yunlong Pei, Hengzhu Zhang & Yuping Li
Gut microbiota is associated with the growth of various tumors, including malignant gliomas, through the brain-gut axis. Moreover, the gut microbiota in patients with malignant tumors may considerably differ from those with benign tumors. However, the associations of gut microbiota with benign and malignant brain tumors remain unclear. Hence, in order to explore these underlying relationships, patients with benign meningioma (n = 32), malignant glioma (n = 27), and healthy individuals (n = 41) were...

Intercity innovation collaboration and the role of high-speed rail connections: evidence from Chinese co-patent data

Li Yao & Jun Li
This study explores the extent to which changes in transport infrastructure counterbalance pre-existing geographical friction and foster innovation collaboration, using the Chinese high-speed rail (HSR) construction as a quasi-natural experiment. Using a comprehensive dataset of city-pair co-patents from 2005 to 2018, we show that HSR connections significantly increase intercity co-patents, patent quality and collaborative partnerships, and such effects are strongest for city-pairs within 250 km and decrease for longer distances. Moreover, the HSR effect is...

A transcriptomic analysis of neuropathic pain in the anterior cingulate cortex after nerve injury

Yu Zhang, Shiwei Jiang, Fei Liao, Zhifeng Huang, Xin Yang, Yu Zou, Xin He, Qulian Guo & Changsheng Huang
The anterior cingulate cortex (ACC) is a core brain region processing pain emotion. In this study, we performed RNA sequencing analysis to reveal transcriptomic profiles of the ACC in a rat chronic constriction injury (CCI) model. A total of 1628 differentially expressed genes (DEGs) were identified by comparing sham-operated rats with rats of 12 hours, 1, 3, 7, and 14 days after surgery, respectively. Although these inflammatory-related DEGs were generally increased after CCI, different kinetics...

Hyperthermia promotes exosome secretion by regulating Rab7b while increasing drug sensitivity in adriamycin-resistant breast cancer

Di Xu, Wen-Juan Tang, Yi-Zhi Zhu, Zhen Liu, Kai Yang, Ming-Xing Liang, Xiu Chen, Yang Wu, Jin-Hai Tang & Wei Zhang
To investigate the mechanism through which hyperthermia promotes exosome secretion and drug sensitivity in adriamycin-resistant breast cancer. We first evaluated the effect of hyperthermia on adriamycin-resistant breast cancer viability and used transmission electron microscopy, nanoparticle tracking analysis, and a bicinchoninic acid kit to validate the effect of hyperthermia on exosome secretion. The effective targeting molecules and pathways changed by hyperthermia were explored by RNA microarray and verified in vitro. The adriamycin-resistant MCF-7/ADR cells co-incubated with...

Phospholipase C is a novel regulator at the early stages of microspore embryogenesis in Nicotiana tabacum

Pan Luo, Aixi Jiang, Yi Zhou, Mingchun Yang, Xiaotong Zhou, Yong Yang, Jun Yu & Xingchun Tang
Microspore transfers the developmental fate into embryogenesis in vitro regulated by determinant factors of stress-induced. However, the key regulators of microspore embryogenesis (ME) are still largely undiscovered to reveal the mechanism of cell fate transition. Here, we report that Phospholipase C (PLC) is involved at the early stages of ME in Nicotiana tabacum. NtPLC2/3/4 are expressed at the initial stages of ME. The expression levels of NtPLC2/3 are transient activated after 3 days in culture,...

A multi-omics investigation into the mechanisms of hyper-virulence in Mycobacterium tuberculosis

Rahim Rajwani, Chala Galata, Annie Wing Tung Lee, Pui-Kin So, Kenneth Siu Sing Leung, Kingsley King Gee Tam, Sheeba Shehzad, Timothy Ting Leung Ng, Li Zhu, Hiu Yin Lao, Chloe Toi-Mei Chan, Jake Siu-Lun Leung, Lam-Kwong Lee, Kin Chung Wong, Wing Cheong Yam & Gilman Kit-Hang Siu
Clinical manifestations of tuberculosis range from asymptomatic infection to a life-threatening disease such as tuberculous meningitis (TBM). Recent studies showed that the spectrum of disease severity could be related to genetic diversity among clinical strains of Mycobacterium tuberculosis (Mtb). Certain strains are reported to preferentially invade the central nervous system, thus earning the label “hypervirulent strains”.However, specific genetic mutations that accounted for enhanced mycobacterial virulence are still unknown. We previously identified a set of 17...

Registration Year

  • 2022
    3,087

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  • Text
    3,087

Affiliations

  • Sichuan University
    3,087
  • Chinese Academy of Medical Sciences & Peking Union Medical College
    2,125
  • Shanghai Jiao Tong University
    1,906
  • Fudan University
    1,768
  • Zhejiang University
    1,714
  • Sun Yat-sen University
    1,601
  • Capital Medical University
    1,497
  • Chinese Academy of Sciences
    1,459
  • Huazhong University of Science and Technology
    1,412
  • Southern Medical University
    1,254