The Sichuan basin has a great potential for hydrocarbon exploration. In recent years, Permian strata have been identified as one of the main target strata for natural gas exploration in the Sichuan basin. Not only the source rocks are abundant, but also there are good reservoirs due to the wide and complex continental oceanic interaction. However, there are still many shortcomings, especially the relationship between the tectonic evolution and the hydrocarbon accumulation model. Therefore, this...

Additional file 1: Figure S1. Characterization of BMSCs. A Morphology of BMSCs under light microscope; B, C Flow cytometry and statistical analysis of the positive rates of MSC-specific surface markers (CD29 and CD90) and a hematopoietic marker CD45 (n = 3); D The analysis of ALP level after osteogenesis induction 14d(n = 3); E The level of mineralized nodules analyzed with Alizarin red staining after osteogenesis induction 21d (n = 3); F The level of...

Additional file 2: Table S2. Hardy-Weinberg Equilibrium Test of rs28742109, rs12955018, rs987850, rs8093805, rs12965084 and rs145497186 that related to NDUFV2.

Additional file 2: Figure S1. Time-kill kinetics of polymyxin B (PolyB; 1 mg/L), rifampicin (Rif; 2 mg/L), and their combination against P. aeruginosa PAO1 at starting 600 nm (OD600) of ~ 0.5 (~ 108 CFU/mL). Figure S2. GO enrichment analysis of the significantly changed genes with the polymyxin B/rifampicin combination at (A) 1 h and (B) 24 h: (i) downregulation and (ii) upregulation of various biological processes. Figure S3. Overview of metabolic pathways of P....

Additional file 2: Table S1. Treatment administration. Table S2. Univariate and multivariate analysis of overall survival and progression-free survival. Table S3. Univariate and multivariate analysis of overall survival and progression-free survival in the PSM cohort. Table S4. Intrahepatic tumor response. Table S5. Baseline characteristics and tumor response within patients performing ELISA detection.

Additional file 4: Figure S2. Kaplan-Meier curves of overall survival in BCLC stage C patients receiving HAIC alone (A), HAIC + TKIs (B) and HAIC + TKIs + ICIs (C) in PSM cohort. Kaplan-Meier curves of progression-free survival in BCLC stage C patients receiving HAIC alone (D), HAIC + TKIs (E) and HAIC + TKIs + ICIs (F) in PSM cohort. CI, confidence interval; HR. hazard ratio; PSM, propensity score matching.

Additional file 1: Figure S1. (A) The transfection efficiencies of sheL31-1 and sheL31-2 in HCT 116 cells were detected by qRT-PCR. (B) The transfection efficiencies of sheL31-1 and sheL31-2 in HCT 116 and RKO cells were evaluated through observing the fluorescence inside cells. Magnification times: 200×. (C) The densitometric analyses of Akt, P-Akt, CCND1, CDK6 and PIK3CA proteins expression. * P < 0.05, *** P < 0.001. Figure S2. (A) The volcano plot of gene...

Supplementary Material 1

Supplementary Material 1

Additional file 1: Table S1. Primers used for strain construction and verification in this study. Table S2. Primers used for qPCR in this study. Table S3. Copy numbers of poman5A gene in the constructed strains measured by qPCR. Figure S1. Map of plasmid pM5p.

Additional file 1: Table S1. Scores of spontaneous pain-related physical parameters.

Additional file 2: Table S2. Immunohistochemical reaction protocols.

As a member of the PGAMs family, PGAM2 has been proved to catalyze the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. However, it is unclear whether PGAM2 has a role in regulating differentiation in goat intramuscular preadipocytes. Here, this study was carried to clone the open reading frame (ORF) of goat PGAM2, elucidate its molecular and expressional characteristics, and evaluate the involvement in adipogenesis of intramuscular preadipocytes. According to our...

As a member of the PGAMs family, PGAM2 has been proved to catalyze the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. However, it is unclear whether PGAM2 has a role in regulating differentiation in goat intramuscular preadipocytes. Here, this study was carried to clone the open reading frame (ORF) of goat PGAM2, elucidate its molecular and expressional characteristics, and evaluate the involvement in adipogenesis of intramuscular preadipocytes. According to our...

supplemental document

Additional file 3. Additional methods. Pathway analysis of the prioritized genes, the databases, and the dataset.

Additional file 3. Additional methods. Pathway analysis of the prioritized genes, the databases, and the dataset.

Additional file 5. Fig. S1. Gene ontology analysis of the 1249 candidate genes (1129 matched).

Additional file 9. Fig. S2. Interaction analyses of prioritized genes by different tools.

Additional File 1. Figure S1: A representative CT image at the third lumbar vertebra (L3) marked with different body composition. Skeletal muscle (SM) in red; subcutaneous adipose tissue (SAT) in blue; visceral adipose tissue (VAT) in yellow; intramuscular adipose tissue (IMAT) in green. Figure S2: Gene expression distributions of each sample for RNA sequencing. Figure S3: Representative images of SAT and VAT from normal and cachectic mice.

Additional file 11. High-quality, free open access resources for point-of-care ultrasound education

Additional file 2. Pre-course knowledge assessment of the learners

Additional file 1: Table S1. Taxa, distribution, and GenBank accession numbers for the sequences used in this study. Table S2. Comparison of the fit of different models of biogeographical range evolution and model-specific estimates for the different parameters. Table S3. Manual dispersal multipliers. Table S4. Georeferenced records of D. sinica used for species distribution modeling.

Additional file 1: Table S1. Taxa, distribution, and GenBank accession numbers for the sequences used in this study. Table S2. Comparison of the fit of different models of biogeographical range evolution and model-specific estimates for the different parameters. Table S3. Manual dispersal multipliers. Table S4. Georeferenced records of D. sinica used for species distribution modeling.

Additional file 2: Fig. S1. Evolutionary inference of nutrition types for Neottieae. Numbers in bold near branches indicate the node number, as referred to Fig. 3. Large pie charts show the relative probabilities of alternative ancestral states obtained by BayesTraits. Fig. S2. Ancestral range reconstruction for Neottieae using BioGeoBEARS under the statistical DEC model. Numbers in bold near branches indicate the node number, as referred to Fig. 3. Large pie charts show the relative probabilities...