A large number of extracts of medicinal plants or natural products shows beneficial to combat obesity. In the present work, a new flavonoid named (2S,1″R,2″R)-4′-hydroxy-7-methoxy-6-(1,2,3-trihydroxy-3-methyl-butyl)-flavanone (1), along with seven known compounds (2–8) were isolated from the seeds of Cullen corylifolium. Their structures, including the absolute configurations, were determined by the analysis of comprehensive spectroscopic data and computational calculation methods. All isolates were evaluated for their diacylglycerol acyltransferase (DGAT) inhibitory activity. Compounds 1–4 exhibited different level...

An analysis method was developed for the separation and quantification of hesperidin, neohesperidin, neohesperidin dihydrochalcone and hesperetin by using HPLC-UV. Single factor experiments and Box-Behnken Designs were used to optimize separation of four flavonoids, in which a gradient elution method was adopted with 99% acetonitrile and 0.1% formic acid as mobile phases at a flow rate of 0.9 mL/min. A C18 column was used with a column temperature of 35 °C. LODs and LOQs were...

Kidney injury molecule 1 (KIM-1) is markedly upregulated in the proximal tubule after injury and is maladaptive when chronically expressed. A practical sensitive chemiluminescence immunoassay combined with magnetic bead separation for the determination of KIM-1 in human urine has been developed. Based on a sandwich-type immunoassay, the analyte is captured by the monoclonal antibodies (mAb) coated in magnetic beads and those labeled with the alkaline phosphatase. The changes of KIM-1 concentration levels were monitored by...

Supplemental material, sj-pdf-1-cpj-10.1177_00099228221134441 for Electrocardiographic QTc as a Surrogate Measure of Cardiac Risk in Children, Adolescents, and Young Adults With Eating Disorders by Perry B. Dinardo, Ellen S. Rome, Ira B. Taub, Wei Liu, Kenneth Zahka and Peter F. Aziz in Clinical Pediatrics

A longstanding challenge with high Mg content Al alloys is the trade-off between corrosion resistance and strength, because the stabilization temperature is lower than the recrystallization temperature corresponding to a no stabilization temperature range. Herein, a stabilization temperature range is found with an excellent match of strength and corrosion resistance of high Mg content Al alloy via pulsed electric current. The stimulated atomic diffusion rate resulted in discontinuous grain boundary precipitates, along with a lower...

Additional file 1: Figure S1. Alignment of the CPGs of sixsubgenus Gynopodium species by Mauve. The Magnolia omeiensis genome (the reference genome) is shown at the top. Color bars indicate locally collinear blocks, and connecting lines indicate correspondingblocks across genomes.

Additional file 3: Figure S3. Phylogenetic relationship of the family Magnoliaceae (20 Magnolia species and two Liriondendron species) based on the CPGs.Phylogenies were inferred by maximum likelihood analysis. Numbers above thelines indicate the bootstrap values from the maximum likelihood analysis.

Supplementary Material 1

Additional file 1: Figure S1. The Tamura 3-parameter model T92+G+I was used to produce theMaximum likelihood tree of 16S rRNA.

Adherence to the use of child restraint system (CRS) can greatly reduce child casualties in road traffic accidents. This study aims to develop the behavior maintenance model of CRS use based on the health action process approach (HAPA) theory to explore the maintenance mechanisms, suggesting appropriate intervention strategies. Adopting the multi-stage stratified cluster random sampling method, a cross-sectional survey for guardians recruited from kindergarten and primary school was conducted using the questionnaire developed based on...

To observe the effect of ultrasound-guided platelet-rich plasma (PRP) injection in the treatment of herpes zoster neuralgia (HZN). Eighty patients with HZN were randomly divided into observation group and control group, with 40 cases in each group. The observation group was treated with ultrasound-guided PRP injection of target nerves combined with drugs. The control group was treated with drugs alone. The pain scores of before treatment (T0), and 1 week (T1), 1 month (T2), 3...

This paper first considers some testing issues for a vector of high-dimensional time series before it establishes a joint distribution for the largest eigenvalues of the

Additional file 5. Original images of Western blots in Fig. 2D, Fig. 3D, Fig. 3E, Fig. 3G, and Fig. 4B, accompanied with brief instructions.

Supplemental material, sj-pdf-1-smm-10.1177_09622802221129042 for Standardization of continuous and categorical covariates in sparse penalized regressions by Xiang Li, Yong Ma and Qing Pan in Statistical Methods in Medical Research

Supplemental material, sj-pdf-1-smm-10.1177_09622802221129042 for Standardization of continuous and categorical covariates in sparse penalized regressions by Xiang Li, Yong Ma and Qing Pan in Statistical Methods in Medical Research

Supplemental material, sj-docx-1-cre-10.1177_02692155221135729 for Effect of respiratory muscle training in patients with chronic kidney disease: A systematic review and meta-analysis of randomized controlled trials by Fan Zhang, Hui Wang, Yan Bai, Liuyan Huang and Huachun Zhang in Clinical Rehabilitation

Supplemental material, sj-docx-2-cre-10.1177_02692155221135729 for Effect of respiratory muscle training in patients with chronic kidney disease: A systematic review and meta-analysis of randomized controlled trials by Fan Zhang, Hui Wang, Yan Bai, Liuyan Huang and Huachun Zhang in Clinical Rehabilitation

Supplemental material, sj-docx-5-cre-10.1177_02692155221135729 for Effect of respiratory muscle training in patients with chronic kidney disease: A systematic review and meta-analysis of randomized controlled trials by Fan Zhang, Hui Wang, Yan Bai, Liuyan Huang and Huachun Zhang in Clinical Rehabilitation

Supplemental material, sj-docx-1-hol-10.1177_09596836221131694 for Hydro-climatic aspects of prehistoric human dynamics in the drylands of the Asian interior by Kangkang Li, Xiaoguang Qin, Bing Xu, Zhiqiang Yin, Yong Wu, Guijin Mu, Dong Wei, Xiaohong Tian, Huiqiu Shao, Chunxue Wang, Hongjuan Jia, Wen Li, Haoze Song, Jiaqi Liu and Yingxin Jiao in The Holocene

Additional file 12: Supplementary Table 1. The clinical information of six LUAD patients accepted the PD1 blockade and underwent circRNA-seq of the peripheral blood. Supplementary Table 2. The clinical characteristics of 113 LUAD patients with different expression of circZNF451. Supplementary Table 3. Target sequences of shRNA and siRNA used in this study. Supplementary Table 4. The antibodies, ELISA kits and drugs used in this study. Supplementary Table 5. The primers and probes used in this...

This report describes fossils recovered from Ganxian Cave in 2008 and 2018 by the Natural History Museum and Anthropology Museum of Guangxi. The cave sedimentary fill yielded rich mammalian fossils consisting mainly of isolated teeth of medium- to large-sized mammals (Primates, Proboscidea, Perissodactyla, Carnivora, Rodentia, Artiodactyla) of typical ‘Ailuropoda-Stegodon’ fauna, (e.g. Stegodon orientalis, Ailuropoda baconi, Pongo weidenreichi, Tapirus sinensis, Megatapirus augustus, Crocuta ultima), and more numerous extant species belonging to 28 taxa in total. The...

Additional file 1. Fig. S1. Optimized lambda selection (A) and feature selection (B) in Lasso regression. Fig. S2. Feature importance values in the Stacking model. Fig. S3. Schematic diagrams of the process for introducing missing values and interpolation in MCAR (A) and MNAR (B). Table S1. Parameter optimization results of KNN and MICE in MCAR. Table S2. Parameter optimization results of KNN and MICE in MNAR. Table S3. The optimized parameters of AE and RRLR....

Additional file 5. Glossary of Terms.

Additional file 5: Supplementary Methods

Additional file 1: Table S1. Clinical improvements in rituximab cohort and control cohort