2,819 Works

An overview of the methods for analyzing the chemical forms of metals in plants

Jiawei Yang, Lin Sun, Xing Shen, Min Dai, Imran Ali, Changsheng Peng & Iffat Naz
Currently, the occurrence of toxic levels of metals in soils is a serious environmental issue worldwide. Phytoremediation is getting much attention to control metals soil pollution because it is economic and environmentally friendly. However, the methods used to detect metals in plants are not uniform and have depicted poor comparability of the research investigations. Therefore, the present overview is designed to discuss the possible chemical forms of metals in various environmental matrixes and the detection...

Regulation of microRNA miR-197-3p/CDC28 protein kinase regulatory subunit 1B (CKS1B) axis by Circular RNA hsa_circ_0000285 promotes glioma progression

Tao Deng, Yang Liu, Yanlong Yang, Leyong Yuan, Fangfang Liu, Xiaobo Wang, Qiuying Zhang & Mingshui Xie
Circular RNA circ_0000285 is differentially expressed in several malignancies; however, its role in gliomas is under investigation. Reverse transcription quantitative polymerase chain reaction was conducted to evaluate the expression of circ_0000285, miR-197-3p, and CDC28 protein kinase regulatory subunit 1B (CKS1B) in glioma tissues and cells. Cell Counting Kit-8 and Transwell invasion assays coupled with Western blotting analysis using anti-Bax and anti-Bcl-2 antibodies were performed to evaluate cell proliferation, invasion, and apoptosis. Luciferase reporter and AGO2...

Nucleosides and amino acids, isolated from Cordyceps sinensis, protected against cyclophosphamide-induced myelosuppression in mice

Yu Zhang, Jie Liu, Yan Wang, Chengpeng Sun, Wenjia Li, Jianjian Qiu, Yanling Qiao, Fan Wu, Xiaokui Huo, Yue An, Baojing Zhang, Shuangcheng Ma, Jian Zheng & Xiaochi Ma
The material basis of Cordyceps sinensis (Berk.) Sacc has not yet been well understood and natural C. sinensis resources are very rare. The present study aimed to clarify the substance basis and compare the protective effect of natural and artificially-cultivated C. sinensis against cyclophosphamide (CTX)-induced myelosuppression. Both natural and artificially-cultivated C. sinensis effectively improved CTX-induced decrease of peripheral blood counts and hemopoietic growth factors, pathological changes, and apoptosis of bone marrow. Importantly, artificially-cultivated C. sinensis...

Intercity innovation collaboration and the role of high-speed rail connections: evidence from Chinese co-patent data

Li Yao & Jun Li
This study explores the extent to which changes in transport infrastructure counterbalance pre-existing geographical friction and foster innovation collaboration, using the Chinese high-speed rail (HSR) construction as a quasi-natural experiment. Using a comprehensive dataset of city-pair co-patents from 2005 to 2018, we show that HSR connections significantly increase intercity co-patents, patent quality and collaborative partnerships, and such effects are strongest for city-pairs within 250 km and decrease for longer distances. Moreover, the HSR effect is...

DNA topoisomerase II alpha promotes the metastatic characteristics of glioma cells by transcriptionally activating β-catenin

Yi Liu, Jun Ma, Jiu-Shan Song, Hai-Ying Zhou, Jing-Hui Li, Cheng Luo, Xin Geng & He-Xiang Zhao
DNA topoisomerase II alpha (TOP2A) reportedly plays a crucial role in several cancers, however, the precise regulatory role of TOP2A in metastatic characteristics of glioma is still poorly understood. Herein, we sought to elucidate the mechanisms by which TOP2A affects the metastatic phenotypes of glioma. We observed that a high level of TOP2A expression was dramatically linked with inferior survival in glioma patients while silencing of TOP2A impaired glioma cell proliferation and aggressiveness. TOP2A was...

DNA topoisomerase II alpha promotes the metastatic characteristics of glioma cells by transcriptionally activating β-catenin

Yi Liu, Jun Ma, Jiu-Shan Song, Hai-Ying Zhou, Jing-Hui Li, Cheng Luo, Xin Geng & He-Xiang Zhao
DNA topoisomerase II alpha (TOP2A) reportedly plays a crucial role in several cancers, however, the precise regulatory role of TOP2A in metastatic characteristics of glioma is still poorly understood. Herein, we sought to elucidate the mechanisms by which TOP2A affects the metastatic phenotypes of glioma. We observed that a high level of TOP2A expression was dramatically linked with inferior survival in glioma patients while silencing of TOP2A impaired glioma cell proliferation and aggressiveness. TOP2A was...

Long non-coding RNA terminal differentiation-induced non-coding RNA regulates cisplatin resistance of choroidal melanoma by positively modulating extracellular signal-regulated kinase 2 via sponging microRNA-19b-3p

Wei Li, Aiqin Nie, Longyu Jin, Yubo Cui, Ning Xie & Gaohua Liang
In the present study, we aimed to investigate the role of long non-coding RNA terminal differentiation-induced non-coding RNA (TINCR) in cisplatin (DDP) resistance of choroidal melanoma (CM) and the potential molecular mechanisms. CM and non-CM tissues were collected from 60 CM patients. DDP-resistant CM cells were obtained by selection with linearly increased DDP treatment. The expression levels of TINCR, microR-19b-3p (miR-19b-3p), and extracellular signal-regulated kinase 2 (ERK-2) were detected by quantitative real-time PCR. Cholecystokinin octapeptide...

AFAP1 antisense RNA 1 promotes retinoblastoma progression by sponging microRNA miR-545-3p that targets G protein subunit beta 1

Wenting Tang, Li Zhang, Jing Li & Yu Guan
The oncogenic role of actin filament-associated protein 1 antisense RNA 1 (AFAP1-AS1) has been reported in retinoblastoma (RB). However, the underlying regulatory mechanisms remain poorly understood. In this study, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and Western blotting were performed to analyze the expression of AFAP1-AS1, microRNA miR-545-3p, or G protein subunit beta 1 (GNB1). Cell Counting Kit-8 (CCK-8) and Transwell migration assays were used to detect cell proliferation and migration. In addition, caspase-3 activity...

Primary graft failure following allogeneic hematopoietic stem cell transplantation: risk factors, treatment and outcomes

Juan Chen, Aiming Pang, Yuanqi Zhao, Li Liu, Runzhi Ma, Jialin Wei, Xin Chen, Yi He, Donglin Yang, Rongli Zhang, Weihua Zhai, Qiaoling Ma, Erlie Jiang, Mingzhe Han, Jiaxi Zhou & Sizhou Feng
Graft failure (GF) is an intractable complication of transplantation, which can severely affect the efficacy of the graft; however, the characteristics, incidence, and risk factors of primary GF have not been well described. This study aimed to analyze the risk factors and outcomes of primary GF to swiftly identify high-risk patients for GF. We performed a case-control study with a case-control ratio of 1:4 with 869 patients who underwent allogeneic hematopoietic stem cell transplantation (allo-HSCT)...

Therapeutic targeting of the USP2-E2F4 axis inhibits autophagic machinery essential for zinc homeostasis in cancer progression

Wenjing Xiao, Jianqun Wang, Xiaojing Wang, Shuang Cai, Yanhua Guo, Lin Ye, Dan Li, Anpei Hu, Shikai Jin, Boling Yuan, Yi Zhou, Qilan Li, Qiangsong Tong & Liduan Zheng
Macroautophagy/autophagy is a conserved cellular process associated with tumorigenesis and aggressiveness, while mechanisms regulating expression of autophagic machinery genes in cancers still remain elusive. Herein, we identified E2F4 (E2F transcription factor 4) as a novel transcriptional activator of cytoprotective autophagy crucial for zinc homeostasis in cancer cells. Gain- and loss-of-function studies showed that E2F4 promoted autophagy in a cell cycle-dependent manner, resulting in facilitated degradation of MT (metallothionein) proteins, elevated distribution of Zn2+ within autophagosomes,...

Therapeutic targeting of the USP2-E2F4 axis inhibits autophagic machinery essential for zinc homeostasis in cancer progression

Wenjing Xiao, Jianqun Wang, Xiaojing Wang, Shuang Cai, Yanhua Guo, Lin Ye, Dan Li, Anpei Hu, Shikai Jin, Boling Yuan, Yi Zhou, Qilan Li, Qiangsong Tong & Liduan Zheng
Macroautophagy/autophagy is a conserved cellular process associated with tumorigenesis and aggressiveness, while mechanisms regulating expression of autophagic machinery genes in cancers still remain elusive. Herein, we identified E2F4 (E2F transcription factor 4) as a novel transcriptional activator of cytoprotective autophagy crucial for zinc homeostasis in cancer cells. Gain- and loss-of-function studies showed that E2F4 promoted autophagy in a cell cycle-dependent manner, resulting in facilitated degradation of MT (metallothionein) proteins, elevated distribution of Zn2+ within autophagosomes,...

Design, synthesis and biological evaluation of a series of dianilinopyrimidines as EGFR inhibitors

Longjia Yan, Qin Wang, Li Liu & Yi Le
This paper described our efforts to develop dianilinopyrimidines as novel EGFR inhibitors. All the target compounds were tested for inhibitory effects against wild type EGFR (EGFRwt) and three tumour cells, including A549, PC-3, and HepG2. Some of the compounds performed well in antitumor activities. Especially, compound 4c 2-((2-((4-(3-fluorobenzamido)phenyl)amino)-5-(trifluoromethyl) pyrimidin-4-yl)amino)-N-methylthiophene-3-carboxamide showed higher anti-tumour activities than Gefitinib. The IC50 values of compound 4c against A549, PC-3, and HepG2. reached 0.56 μM, 2.46 μM, and 2.21 μM, respectively....

Combined radiofrequency ablation or microwave ablation with transarterial chemoembolization can increase efficiency in intermediate-stage hepatocellular carcinoma without more complication: a systematic review and meta-analysis

Yao Yang, Hongli Yu, Lingyu Qi, Chenxi Liu, Yuemin Feng, Jianni Qi, Jie Li & Qiang Zhu
Radiofrequency ablation (RFA) and microwave ablation (MWA) are widely used in combination with transarterial chemoembolization (TACE) for intermediate-stage hepatocellular carcinoma (HCC) in clinical practice. We aim to compare the efficacy and safety of TACE combined with RFA or MWA versus TACE monotherapy for intermediate-stage HCC. We searched PubMed, Embase, Web of Science, and the Cochrane Library for relevant studies. The outcomes included overall survival (OS), progression-free survival (PFS), local tumor control (LTC) rate, and major...

The role of gut microbiota in patients with benign and malignant brain tumors: a pilot study

Haixiao Jiang, Wei Zeng, Xiaoli Zhang, Yunlong Pei, Hengzhu Zhang & Yuping Li
Gut microbiota is associated with the growth of various tumors, including malignant gliomas, through the brain-gut axis. Moreover, the gut microbiota in patients with malignant tumors may considerably differ from those with benign tumors. However, the associations of gut microbiota with benign and malignant brain tumors remain unclear. Hence, in order to explore these underlying relationships, patients with benign meningioma (n = 32), malignant glioma (n = 27), and healthy individuals (n = 41) were...

UHPLC-QTOF-MS/MS based characterization of anti-tumor constituents in Ceratocarpus arenarius L. and identification of EGFR-TK inhibitors by virtual screening

Le Pan, Li Li, Lu Xu, Jiaojiao Zhang, Jiashan Li, Ming Gao, JinMen Yu, Lu Jin & Dongyu Lei
The constituents of Ceratocarpus arenarius L., as a traditional anticancer medicine of Kazakh, were firstly profiled with UHPLC-QTOF-MS/MS. The potential compounds against EGFR-TK were virtually screened. As a result, forty-four compounds were analyzed, including 18 flavonoids, 8 steroids, 4 phenolic acids, 9 fatty acids, 1 coumarin and 4 other compounds. Among them, 9 flavonoids, N-trans-Feruloyltyramine (5), stigmasterol (11) and carthamone (38) were recognized as potential key anti-tumor constituents of C. arenarius through docking to active...

Reproducibility of the Infinium methylationEPIC BeadChip assay using low DNA amounts

Steffan Noe Christiansen, Jeppe Dyrberg Andersen, Marie-Louise Kampmann, Jing Liu, Mikkel Meyer Andersen, Jacob Tfelt-Hansen & Niels Morling
The Infinium MethylationEPIC BeadChip (EPIC) is a reliable method for measuring the DNA methylation of more than 850,000 CpG positions. In clinical and forensic settings, it is critical to be able to work with low DNA amounts without risking reduced reproducibility. We evaluated the EPIC for a range of DNA amounts using two-fold serial dilutions investigated on two different days. While the β-value distributions were generally unaffected by decreasing DNA amounts, the median squared Pearson’s...

Identification and potential mechanism of different components from the aerial part of Bupleurum chinense DC. for epileptic treatment

Xiaomao Li, Yan Liu, Siyi Wang, Wei Guan, Juan Pan, Haixue Kuang & Bingyou Yang
The present study aimed to assess the effects of epileptic treatment of the aerial part of Bupleurum chinense DC. in kainic acid (KA)-induced epilepsy rats and LPS-induced BV2 cells, as well as to identify the active chemical constituents. The in vivo and vitro results showed that 20% ethanol elution fractions of the aerial part of B. chinense DC. (BCE-20) and 70% ethanol elution fractions of the aerial part of B. chinense DC. (BCE-70) could improve...

Network pharmacology and in vitro experimental verification to explore the mechanism of Sanhua decoction in the treatment of ischaemic stroke

Wei Zhang, Li Zhang, Wen jun Wang, Shanbo Ma, Mingming Wang, Minna Yao, Ruili Li, Wei wei Li, Xian Zhao, Dongmei Hu, Yi Ding & Jingwen Wang
Stroke is an illness with high morbidity, disability and mortality that presents a major clinical challenge. Sanhua decoction (SHD) has been widely used to treat ischaemic stroke in the clinic. However, the potential mechanism of SHD remains unknown. To elucidate the multitarget mechanism of SHD in ischaemic stroke through network pharmacology and bioinformatics analyses. Network pharmacology and experimental validation approach was used to investigate the bioactive ingredients, critical targets and potential mechanisms of SHD against...

Synthesis and bioactivities evaluation of oleanolic acid oxime ester derivatives as α-glucosidase and α-amylase inhibitors

Xu-Yang Deng, Jun-Jie Ke, Ying-Ying Zheng, Dong-Li Li, Kun Zhang, Xi Zheng, Jing-Ying Wu, Zhuang Xiong, Pan-Pan Wu & Xue-Tao Xu
Different oleanolic acid (OA) oxime ester derivatives (3a-3t) were designed and synthesised to develop inhibitors against α-glucosidase and α-amylase. All the synthesised OA derivatives were evaluated against α-glucosidase and α-amylase in vitro. Among them, compound 3a showed the highest α-glucosidase inhibition with an IC50 of 0.35 µM, which was ∼1900 times stronger than that of acarbose, meanwhile compound 3f exhibited the highest α-amylase inhibitory with an IC50 of 3.80 µM that was ∼26 times higher...

A transcriptomic analysis of neuropathic pain in the anterior cingulate cortex after nerve injury

Yu Zhang, Shiwei Jiang, Fei Liao, Zhifeng Huang, Xin Yang, Yu Zou, Xin He, Qulian Guo & Changsheng Huang
The anterior cingulate cortex (ACC) is a core brain region processing pain emotion. In this study, we performed RNA sequencing analysis to reveal transcriptomic profiles of the ACC in a rat chronic constriction injury (CCI) model. A total of 1628 differentially expressed genes (DEGs) were identified by comparing sham-operated rats with rats of 12 hours, 1, 3, 7, and 14 days after surgery, respectively. Although these inflammatory-related DEGs were generally increased after CCI, different kinetics...

Network pharmacology and in vitro experimental verification to explore the mechanism of Sanhua decoction in the treatment of ischaemic stroke

Wei Zhang, Li Zhang, Wen jun Wang, Shanbo Ma, Mingming Wang, Minna Yao, Ruili Li, Wei wei Li, Xian Zhao, Dongmei Hu, Yi Ding & Jingwen Wang
Stroke is an illness with high morbidity, disability and mortality that presents a major clinical challenge. Sanhua decoction (SHD) has been widely used to treat ischaemic stroke in the clinic. However, the potential mechanism of SHD remains unknown. To elucidate the multitarget mechanism of SHD in ischaemic stroke through network pharmacology and bioinformatics analyses. Network pharmacology and experimental validation approach was used to investigate the bioactive ingredients, critical targets and potential mechanisms of SHD against...

Bioactive polyacetylenes from Bidens pilosa L and their anti-inflammatory activity

Zhi Yan, Zhenshan Chen, Ling Zhang, Xiaoming Wang, Yaowen Zhang & Zhenhua Tian
A new polyacetylenic glucoside together with three known compounds were isolated from the whole herb of Bidens pilosa L. The structure elucidation of these four compounds was employed by combining NMR and HR-MS data as 4-O-β-D-glucopyranosyloxy-1-hydroxy- 6-(E)-tetradecene-8,10,12-triyne (1), 3-O-β-D-glucopyranosyloxy-1-hydroxy-6-(E)-tetradec- ene-8,10,12-triyne (2), 2-O-β-D-glucopyranosyloxy-1-hydroxy-5-(E)-tetradecene-7,9,11-triy- ne (3) and icthyothereol acetate (4). Additionally, bioactivity study showed that these compounds have potential anti-inflammatory activity. This study has certain guiding significance for the development and utilisation of polyacetylene compounds in Bidens...

Hyperthermia promotes exosome secretion by regulating Rab7b while increasing drug sensitivity in adriamycin-resistant breast cancer

Di Xu, Wen-Juan Tang, Yi-Zhi Zhu, Zhen Liu, Kai Yang, Ming-Xing Liang, Xiu Chen, Yang Wu, Jin-Hai Tang & Wei Zhang
To investigate the mechanism through which hyperthermia promotes exosome secretion and drug sensitivity in adriamycin-resistant breast cancer. We first evaluated the effect of hyperthermia on adriamycin-resistant breast cancer viability and used transmission electron microscopy, nanoparticle tracking analysis, and a bicinchoninic acid kit to validate the effect of hyperthermia on exosome secretion. The effective targeting molecules and pathways changed by hyperthermia were explored by RNA microarray and verified in vitro. The adriamycin-resistant MCF-7/ADR cells co-incubated with...

Effects of glycerol and glucose on docosahexaenoic acid synthesis in Aurantiochyrium limacinum SFD-1502 by transcriptome analysis

Huaqiu Zhang, Xiangying Zhao, Chen Zhao, Jiaxiang Zhang, Yang Liu, Mingjing Yao & Jianjun Liu
Docosahexaenoic acid (DHA) has numerous functions in adjusting the organic health and pragmatic value in medicine and food field. In this study, we compared glycerol and glucose as the only carbon source for DHA production by Aurantiochytrium. When the glycerol concentration was 120 g/L, the maximum DHA yield was 11.08 g/L, and the DHA yield increased significantly, reaching 47.67% of the total lipid content. When the cells grew in glucose, the DHA proportion was 37.39%....

Water-soluble chitosan and phytoremediation efficiency of two Brassica napus L. cultivars in cadmium-contaminated farmland soils

Jian-Lin Bian, Wei Cao, Jun-Mei Guo, Jun-Xing Yang, Xue-Dong Wang, Jie Wang, Jun Huang, Tian-Xiang Xia & Cun-Yan Xia
Pot and field trials were conducted to investigate Cd uptake and phytoremediation efficiency of two Brassica napus cultivars (QY-1 and SYH) with applied water-soluble chitosan (WSC, Pot: 0, 2% and 4%; Field: 0 and 10 g·m−2) grown in Cd-contaminated soils. The results from the pot and field trials generally showed that WSC treatments significantly increased Cd concentrations in shoot and root tissues by 33.77–159.71% (except for SYH/JY) and 7.42–168.71% of two B. napus cultivars compared...

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  • 2022
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Affiliations

  • Capital Medical University
    2,819
  • Chinese Academy of Medical Sciences & Peking Union Medical College
    1,798
  • Shanghai Jiao Tong University
    1,777
  • Zhejiang University
    1,591
  • Fudan University
    1,585
  • Sichuan University
    1,499
  • Sun Yat-sen University
    1,451
  • Huazhong University of Science and Technology
    1,335
  • Chinese Academy of Sciences
    1,314
  • Nanjing Medical University
    1,239