Additional file 9: Table S7. Subgroup analysis of the relation between clinical factors and overall survival (OS)

Additional file 10: Table S8. Subgroup analysis of the correlation between clinical factors and overall response rate (ORR)

Additional file 2: Fig. S1. Sample collection, single-cell clustering and marker gene visualization for major lineages. Fig. S2. Immunohistochemistry (IHC) staining of immune cells. Fig. S3. Cluster marker genes, tumor cell identification, cellular fractions and hematoxylin eosin (H&E) staining for epithelial cells. Fig. S4. Upregulated genes and signatures for malignant cells among TN, MPR and NMPR patients and serum estradiol dynamics. Fig. S5. Marker genes, differentially expressed genes and RNA velocity for T/NK subsets. Fig....

Additional file 1: Supplementary data 1. No significant differences in laboratory parameters between serous and mucinous BEOT patients.

Additional file 2: Supplementary data 2. Comparison of AUC of single and combined use of CA125, NLR and PLR.

Additional file 2: Table S1. Independent genome-wide significant lead SNPs associated with pulmonary embolism (PE) in the discovery stage and replication stage. Table S2. The allele frequency of identified loci in 1000Genomes. Table S3. Replication of associations for the known loci in our cohort. Table S4. Association results for genes that were significant in FUMA gene-based analysis. Table S5. Polygenic risk score variants. Table S6. Polygenic risk score (PRS) quantile and odds ratio (OR).

Additional file 2: Table S2. Surgical evaluation before and after neoadjuvant therapy.

Additional file 2: Supplementary Figure 1. The relative expression levels of LINC00240 in human GES-1, MKN-28, MKN-45, AGS, BGC-823, HGC-27 and MGC-803 cell lines. ***P < 0.001. Supplementary Figure 2. Silencing of LINC00240 significantly promoted apoptosis of gastric cancer cells (A), but did not impact cell cycle (B). Supplementary Figure 3. Expression of apoptotic proteins (A), DDX21 and USP10 (B) in gastric cancer xenografts.

Supplemental material, sj-docx-1-jic-10.1177_08850666231170778 for Different Concentrations of Albumin Versus Crystalloid in Patients with Sepsis and Septic Shock: A Meta-Analysis of Randomized Clinical Trials by Li Geng, Xiaoxue Tian, Zifeng Gao, Aiqin Mao, Lei Feng and Chao He in Journal of Intensive Care Medicine

Additional file 4. Images of the original blots presented in this study.

Additional file 1: Fig. S1. Peptides translated by detected circRNAs are present in mouse testes. a. The specific peptides translated by circRNAs were detected by mass spectrometry and the corresponding spectra are shown. Fig. S2. The detected circRNAs are present in mouse testes. a. Linear splicing forms of the gene of interest were detected specifically in mouse testes by convergent primers. Both divergent primers detected circular forms in cDNA but not in gDNA of mouse...

Additional file 1: Figure S1. Flowchart of the patient enrollment in this study. Figure S2. Survival analysis of the cell densities of intratumoral-infiltrating CD8 + T-cell subsets in patients with NSCLC. Figure S3. The risk-correlation analysis of recurrence-free survival based on the density of CD8 + T-cell subsets in NSCLC. Figure S4. The risk-correlation analysis of lymph node metastases based on the mean nearest neighbor distance between CD8 + T cells and neighboring cells concerned...

Additional file 2. Overview of stained sections from all tissue microarray before and after fluorescence imaging.

Supplementary Table S1: ROB 2.0 for quality assessment of RCTs

Supplementary Material 4

Supplementary Material 4

Spermatogenesis is an important physiological process associated with male infertility. As a kind of post-transcriptional regulation, RNA editings (REs) change the genetic information at the mRNA level. But whether there are REs and what’s the role of REs during the process are still unclear. In this study, we integrated published RNA-Seq datasets and established a landscape of RNA REs during the development of mouse spermatogenesis. Totally, 7530 editing sites occurred in 2012 genes among all...

Additional file 1: Fig S1.

This phase III clinical trial aimed to assess the safety and demonstrate the immunogenicity of a candidate freeze-dried purified Vero cell-based rabies vaccine (PVRV-WIBP) developed for human use. A cohort of 40 participants in stage 1 and 1956 subjects in stage 2 with an age range of 10–50 years were recruited for the phase III clinical trial. For safety analysis in stage 1, 20 participants received either 4-dose or 5-dose regimen of PVRV-WIBP. In stage...

Additional file 3: Table S1. The number of experimental animals.

Additional file 2: Table S1. Independent genome-wide significant lead SNPs associated with pulmonary embolism (PE) in the discovery stage and replication stage. Table S2. The allele frequency of identified loci in 1000Genomes. Table S3. Replication of associations for the known loci in our cohort. Table S4. Association results for genes that were significant in FUMA gene-based analysis. Table S5. Polygenic risk score variants. Table S6. Polygenic risk score (PRS) quantile and odds ratio (OR).

Supplementary Material 1

Two new tigliane- and daphnane-type diterpenoids, given the trivial names daphnegens A–B (1–2) were isolated from the buds of Daphne genkwa. Their structures were assigned on the basis of extensive spectroscopic. The absolute configurations of both compounds were determined by comparison of their calculated and experimental CD curves. In addition, compounds 1–2 were tested for their cytotoxic activities against MCF-7 and HepG-2 human cancer cell lines, and compound 2 showed remarkable cytotoxic activity against HepG-2...

Drug resistance has greatly limited the clinical efficacy of lenvatinib in hepatocellular carcinoma (HCC). However, the underlying molecular mechanisms of lenvatinib resistance remain largely undetermined. Further in-depth exploration of mechanisms underlying lenvatinib resistance is still required for the majority of HCC patients. In this study, an integrated unbiased whole-genome CRISPR-Cas9 screen with database analysis indicated LAPTM5 (lysosomal protein transmembrane 5) as the critical contributor to lenvatinib resistance in HCC. We revealed that LAPTM5 could promote...

IRON-REGULATED TRANSPORTER 1 (IRT1) is critical for iron uptake in roots, and its exocytosis to the plasma membrane (PM) is regulated by the iron status sensed by the histidine-rich domain (HRM). However, studies on the fate of IRT1 after fusion with PM in response to iron conditions are still limited. In this study, we found that K165 and K196 regulate the monoubiquitination of MxIRT1 (mUb-MxIRT1), which acts as a receptor delivering signals from HRM to...