Lentiviruses harbour high genetic variability for efficient evasion from host immunity. An attenuated equine infectious anaemia (EIA) vaccine was developed decades ago in China and presented remarkably robust protection against EIA. The vaccine was recently proven to have high genomic diversity, particular in env. However, how and to what extent the high env diversity relates to immune protection remains unclear. In this study, we compared immune protections and responses of three groups of horses stimulated...

Bovine viral diarrhea virus (BVDV) is the causative agent of bovine viral diarrhea-mucosal disease, which significantly affects the production performance of cattle, causing serious economic losses to the cattle industries worldwide. Up to now, some mechanisms involved in host–BVDV interaction are still not fully understood. The discovery of long non-coding RNAs (lncRNAs) has provided a new perspective on gene regulation in diverse biological contexts, particularly in viral infection and host immune responses. However, little is...

Drug-resistant pathogenic Staphylococcus aureus (S. aureus) has severely threatened human health and arouses widespread concern. Sortase A (SrtA) is an essential virulence factor of S. aureus, which is responsible for the covalent anchoring of a variety of virulence-related proteins to the cell wall. SrtA has always been regarded as an ideal pharmacological target against S. aureus infections. In this research, we have determined that orientin, a natural compound isolated from various medicinal plants, can effectively...

Porcine epidemic diarrhea virus (PEDV) is emerging as a major threat to the global swine industry. Clinical PEDV infection is associated with severe intestinal lesions, resulting in absorptive dysfunction and high mortality rates in suckling piglets. The extracellular matrix (ECM) is an important component of intestinal tissue, providing a structural framework and conveying tissue-specific signals to nearby enterocytes. In this study, we investigated the extensive ECM remodeling observed in intestinal epithelial cells infected with PEDV...

Satellite-based aerosol optical depth (AOD) retrieval products are essential in air pollution and climate change research. Unfortunately, cloud contaminations and unfavorable surface conditions result in a considerable proportion of missing AOD data. Numerous studies have been conducted to reconstruct large-scale AOD data gaps by utilizing adjacent spatiotemporal information or modeling AOD data via various external geographical data. However, the erratic variation of AOD and the inconvenience of external data weaken the accuracy and efficiency of...

Sponge iron (SI) is widely used in water treatment. As effluents from wastewater treatment plant (WWTP) require advanced treatment methodology, three forms of constructed wetlands (CWs): wetlands with sponge iron (SI), copper sulfate modified sponge iron (Cu/SI), and sponge iron coupled with solid carbon sources (C/SI), have been investigated in this paper for the removal effects of organic matter and nutrients in WWTP effluents, and the corresponding mechanisms have been analyzed. The results showed the...

Additional file 1: Table S1. Clinicopathological characteristics of patients with gastric cancer in this study. Table S2. Different microorganisms in Proximal T and Distal T compared to their respective non tumor samples. Table S3. Significant differences in metabolites between Distal T and Distal N. Table S4. Significant differences in metabolites between Proximal T and Proximal N. Table S5. Metabolic pathway enrichment of differential metabolites between Distal T and Distal N. Table S6. Metabolic pathway enrichment...

Additional file 2: Figure S2. The microbial relative abundance of proximal, distal tumor tissues and matched non-tumor tissues at the genus level. Proximal T,proximal GC tumor tissues; Proximal N,proximal GC non-tumor tissues; Distal T, distal GC tumor tissues; Distal N, distal GC non-tumor tissues.

Additional file 2: Figure S2. Selection of the optimal number of clusters.

Additional file 15: Supplementary methods. Description of normalization methods used data normalization.

Additional file 4: Table S2. Performance of the SVM model on tumor types.

Additional file 3. Features of the selected 910 E complete sequences for temporal phylogenic reconstruction covering all spatio-temporal distributions and representing all designated subgenotypes and clades in genotypes I, IV, and V from 5003 DENV-1 strains worldwide.

Suppplement 1

Supplemental material, sj-pdf-2-imr-10.1177_03000605221134467 for Efficacy of median nerve electrical stimulation on the recovery of patients with consciousness disorders: a systematic review and meta-analysis by Peng Wang, Wei Cao, Hong Zhou, Huan Xin Zhang, Lunzhong Zhang, Li Liu, Yunlong Sui, Zhen Zhang, Xiaoyu Yin, Fan Yang and Li Kong in Journal of International Medical Research

Supplemental material, sj-pdf-3-imr-10.1177_03000605221134467 for Efficacy of median nerve electrical stimulation on the recovery of patients with consciousness disorders: a systematic review and meta-analysis by Peng Wang, Wei Cao, Hong Zhou, Huan Xin Zhang, Lunzhong Zhang, Li Liu, Yunlong Sui, Zhen Zhang, Xiaoyu Yin, Fan Yang and Li Kong in Journal of International Medical Research

Supplemental material, sj-pdf-5-imr-10.1177_03000605221134467 for Efficacy of median nerve electrical stimulation on the recovery of patients with consciousness disorders: a systematic review and meta-analysis by Peng Wang, Wei Cao, Hong Zhou, Huan Xin Zhang, Lunzhong Zhang, Li Liu, Yunlong Sui, Zhen Zhang, Xiaoyu Yin, Fan Yang and Li Kong in Journal of International Medical Research

Additional file 1: Fig. S1. TG analysis of POMs, POM-NH2 and GOD@POMs. Fig. S2. UV-vis-NIR absorption of GOD@POMs dispersed in H2O or PBS at 24 and 72 h. Fig. S3. TEM image of aggregated GOD@POMs after reaction with glucose. Fig. S4. UV-vis-NIR spectra and the corresponding digital photo of GOD@POMs dispersed in PBS with different pH values ranging from 4.0 to 7.3. Fig. S5. Glucose concentration and time-dependent oxidation of DPBF by GOD@POMs. Fig. S6....

Additional file 1: Fig. S1. TG analysis of POMs, POM-NH2 and GOD@POMs. Fig. S2. UV-vis-NIR absorption of GOD@POMs dispersed in H2O or PBS at 24 and 72 h. Fig. S3. TEM image of aggregated GOD@POMs after reaction with glucose. Fig. S4. UV-vis-NIR spectra and the corresponding digital photo of GOD@POMs dispersed in PBS with different pH values ranging from 4.0 to 7.3. Fig. S5. Glucose concentration and time-dependent oxidation of DPBF by GOD@POMs. Fig. S6....

Objective: An increasing number of studies suggest that sleep disordered breathing (SDB) may be associated with postoperative atrial fibrillation (POAF), but these studies present discrepant results. Thus, this meta-analysis aimed to synthesize the data associating SDB with POAF in patients who underwent cardiac surgery. Methods: A literature search was performed in the Scopus, PubMed, Web of Science, EMBASE, CENTRAL, Weipu, Wanfang Data, and China National Knowledge Infrastructure databases before August 2022. Data were extracted, and...

Suppplement 1

UV irradiation leads to various usage issues of engineered wood products (EWPs), including discoloration and declined mechanical performance. The radical generation and physico-chemical changes of the wood-phenol formaldehyde (PF) adhesive interphase during alternating UV irradiation were investigated in this paper, aiming to understand the oxidation and degradation of EWPs in daily applications. Electron spin resonance spectroscopy demonstrated that UV irradiation activates the phenol ring in lignin and PF adhesive, generating the reactive oxygen species (ROSs)...

Additional file 10. Figure S7: Prognostic value of presurgical ctDNA detection. A). The recurrence-free survival analysis of patients stratified by presurgical ctDNA detection. B). The multi-variant Cox regression for presurgical ctDNA detection. Abbreviations: RFS – recurrence-free survival, LNM – lymph node metastasis, LUAD – lung adenocarcinoma, LUSC - lung squamous-cell carcinoma.

Additional file 11. Methods.

Additional file 4. Figure S1: Availability of plasma samples. The availability of plasma samples for analysis at each schedule collection time point. Blue and red blocks denote samples collected before and after disease recurrence, respectively. Abbreviations: LUAD – lung adenocarcinoma, LUSC - lung squamous-cell carcinoma, RFS – recurrence-free survival, AT – adjuvant therapy, RT – radiotherapy, LNM – lymph node metastasis.

Additional file 8. Figure S5: Prognostic values of ctDNA detection based on clonal and subclonal mutations. A). The recurrence-free survival analysis of patients stratified by ctDNA detection based on only clonal mutation profiles. B). The recurrence-free survival analysis of patients stratified by ctDNA detection based on all clonal and subclonal mutations.