Additional file 2. Daily dietary and exercise records.

Additional file 2. Daily dietary and exercise records.

An amide-assisted arene nitration is presented, and both mono- and dinitration of protected anilines could be effected by using NaNO2 and NaNO3 as the mono- and bisnitrating agents, respectively. This divergent synthesis is transition-metal- and acid-free, and features a broad substrate scope, low cost, and ortho–para selectivity.

Mobile colistin resistance (mcr) genes represent an emerging challenge. Here we describe a novel mcr gene, mcr-10, on an IncFIA plasmid of an Enterobacter roggenkampii clinical strain. mcr-10 has the highest nucleotide identity (79.69%) with mcr-9 and encodes MCR-10 with 82.93% amino acids identical to MCR-9. mcr-10 confers 4-fold increase in colistin MIC (from 1 to 4 mg/L) when cloned into a colistin-susceptible E. roggenkampii strain. By screening GenBank, mcr-10 was found in various Enterobacteriaceae...

Coronavirus disease 2019 (COVID-19) has a wide spectrum of disease severity from mild upper respiratory symptoms to respiratory failure. The role of neutralizing antibody (NAb) response in disease progression remains elusive. This study determined the seroprevalence of 733 non-COVID-19 individuals from April 2018 to February 2020 in the Hong Kong Special Administrative Region and compared the neutralizing antibody (NAb) responses of eight COVID-19 patients admitted to the intensive care unit (ICU) with those of 42...

Staff and employees “Zero infection” has been achieved during the whole medical activities in the COVID-19 Fangcang Shelter Hospital in Wuhan, China. This study analyses the personnel and environmental protection status of the East–West Lake Fangcang Shelter Hospital. The HCWs were mostly composed of national medical rescue teams, from different provinces in China. Before the COVID-19 outbreak, 82.64% of the HCWs had already known the proper procedure of wearing masks and other personal protective equipment...

Staff and employees “Zero infection” has been achieved during the whole medical activities in the COVID-19 Fangcang Shelter Hospital in Wuhan, China. This study analyses the personnel and environmental protection status of the East–West Lake Fangcang Shelter Hospital. The HCWs were mostly composed of national medical rescue teams, from different provinces in China. Before the COVID-19 outbreak, 82.64% of the HCWs had already known the proper procedure of wearing masks and other personal protective equipment...

Tigecycline is considered one of the last-resort antimicrobials for carbapenem-resistant K. pneumoniae. Plasmid-mediated tigecycline resistance remains largely unclear. Here, by utilizing whole genome sequencing, we report a plasmid-mediated tigecycline resistance mechanism, a 6,489 bp Resistance-nodulation-division family (RND) efflux pump (tmexCD1-toprJ1 pump), that confers transferable tigecycline resistance in K pneumoniae isolated from patients and chickens. In addition, we identified high prevalence of the plasmids co-harbouring both tmexCD1-toprJ1 pump and mcr (tmexCD1-mcr co-harbouring plasmid) from human in...

Tigecycline is considered one of the last-resort antimicrobials for carbapenem-resistant K. pneumoniae. Plasmid-mediated tigecycline resistance remains largely unclear. Here, by utilizing whole genome sequencing, we report a plasmid-mediated tigecycline resistance mechanism, a 6,489 bp Resistance-nodulation-division family (RND) efflux pump (tmexCD1-toprJ1 pump), that confers transferable tigecycline resistance in K pneumoniae isolated from patients and chickens. In addition, we identified high prevalence of the plasmids co-harbouring both tmexCD1-toprJ1 pump and mcr (tmexCD1-mcr co-harbouring plasmid) from human in...

Under the ongoing COVID-19 prevention and control measures in China, increasing the laboratory severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleic acid testing capacity has become the top priority. Since the COVID-19 outbreak in Xinfadi market in Beijing in June 2020, large-scale screening of key populations has been carried out, challenging the nucleic acid testing capabilities of hospital laboratories. Therefore, within 48 hours, Peking University People’s Hospital (PKUPH) transformed a non-nucleic acid testing laboratory into...

A simple, fast, air-assisted liquid-liquid micro-extraction technique using the solidification of a floating organic droplet method was developed for the extraction of three strobilurin fungicides in water samples before analysis by high-performance liquid chromatography equipped with a diode array detector (HPLC-DAD). In this method, a low-density solvent (1-dodecanol) was used as the extraction solvent. Fine extraction solvent droplets were rapidly formed and dispersed into the water samples through air-pumping by syringe rather than an organic...

This study aimed to clone the full-length open reading frame (ORF) of goat ANGPTL8 gene sequence, reveal its molecular and expression characteristics, and explore its effect on the differentiation of goat intramuscular preadipocytes. The full-length ORF sequence of goat ANGPTL8 gene was cloned by RT-PCR technology, and bioinformatics analysis was performed by related biological software. RT-qPCR was used to detect the expression of ANGPTL8 mRNA in goat tissues. Further use of RNA interference to study...

Application of algicidal bacteria is a promising technology to control harmful algal blooms (HABs). In this study, algicidal bacteria strains Shigella sp. H3 and Alcaligenes sp. H5 were obtained via two different isolation methods from the same lake water sample, with optimal algicidal efficiencies 96% and 74% against algae mixture. The Shigella sp. H3 and Alcaligenes sp. H5 lysed algae cells through cells-to-cells direct contact and secretion of algicidal metabolites, respectively. The stronger algicidal capability...

Supplemental Material, sj-pdf-1-teu-10.1177_13548166211021175 for Effects of tourism development on economic growth: An empirical study of China based on both static and dynamic spatial Durbin models by Honghong Liu, Ye Xiao, Bin Wang and Dianting Wu in Tourism Economics

A simple, fast, air-assisted liquid-liquid micro-extraction technique using the solidification of a floating organic droplet method was developed for the extraction of three strobilurin fungicides in water samples before analysis by high-performance liquid chromatography equipped with a diode array detector (HPLC-DAD). In this method, a low-density solvent (1-dodecanol) was used as the extraction solvent. Fine extraction solvent droplets were rapidly formed and dispersed into the water samples through air-pumping by syringe rather than an organic...

Patients with depression have a high prevalence of developing dyslipidemia. In this study, we aim to investigate the difference of serum lipids, including total cholesterol (TCH), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), and triglycerides (TG), between the depressed patients and healthy controls. Sex differences in lipids and their psychological correlations were also included. The study included 56 healthy controls (males/females =26/30) and 110 first-diagnosed drug-naïve outpatients (males/females =35/75). A total of 42 patients...

Supplemental Material, sj-pdf-1-teu-10.1177_13548166211021175 for Effects of tourism development on economic growth: An empirical study of China based on both static and dynamic spatial Durbin models by Honghong Liu, Ye Xiao, Bin Wang and Dianting Wu in Tourism Economics

Jiaxing is a medium-sized city in the Yangtze River Delta (YRD), which showed complex local and surrounding pollution sources. To study the COVID-19 impact on the ambient PM2.5 in Jiaxing, we collected the PM2.5 samples from 2 January to 25 April 2020 and analysed their chemical compositions (including carbon components, water-soluble ions (WSIs), and inorganic elements). The concentration of PM2.5 was 83.13 ± 30.93 μg/m3 before COVID-19 pandemic and then remarkably decreased with COVID-19 outbreak...

Users rely heavily on mobile phones as multi-purpose devices to support all kinds of tasks. This study conceptualises mobile phone deep structure use and aims at unveiling such behaviour. Towards this end, this study goes beyond the traditional technology usage theories based on rationality and instrumentality. It is replaced by a relationship-based theory, the attachment theory, to investigate how users’ attachment to mobile phones is formed and how such attachment influences mobile phone deep structure...

A growing body of evidence suggests the pivotal role of long non-coding RNA (lncRNA) in influenza virus infection. Based on next-generation sequencing, we previously demonstrated that Lnc45 was distinctively stimulated by H5N1 influenza virus in mice. In this study, we systematically investigated the specific role of Lnc45 during influenza A virus (IAV) infection. Through qRT-PCR, we first demonstrated that Lnc45 is highly up-regulated by different subtypes of IAV strains, including H5N1, H7N9, and H1N1 viruses....

A new aromatic glycoside (1) and twelve (2–13) known aromatic glycosides were isolated from the n-butyl alcohol of the 70% EtOH extract of the aerial part of Bupleurum chinense. Among them, compounds 4–7, 9, 11–13 were isolated from Umbelliferae plants for the first time. Their structures were elucidated by NMR and MS spectroscopy, and the absolute configuration of compound 1 was elucidated by single-crystal X-ray diffraction. Compounds 6 and 10 showed moderate cytotoxic activities on...

Recovering phosphorus (P) from excess sludge of wastewater treatment plants (WWTPs) has attracted considerable attention. An efficient P release method is undoubtedly critical for a satisfactory recovery performance. In this study, the effectiveness of three sludge pretreatment methods, i.e. anaerobic digestion (AD), EDTA-anaerobic digestion (EA) and ultrasound combined with EA (U-EA), on P release and struvite recovery from excess sludge was investigated. The results showed that different pretreatment methods resulted in the different characteristics of...

Recovering phosphorus (P) from excess sludge of wastewater treatment plants (WWTPs) has attracted considerable attention. An efficient P release method is undoubtedly critical for a satisfactory recovery performance. In this study, the effectiveness of three sludge pretreatment methods, i.e. anaerobic digestion (AD), EDTA-anaerobic digestion (EA) and ultrasound combined with EA (U-EA), on P release and struvite recovery from excess sludge was investigated. The results showed that different pretreatment methods resulted in the different characteristics of...

Deciphering the transcriptional activities of pathogenicity genes is critical to understand pathogenesis mechanisms of Phytophthora melonis, a devastating pathogen of cucurbits. In this study, we have performed comparative proteomics among P. melonis mutants to evaluate the interacting pathogenicity proteins. Structural characterization of all detected proteins discovered the physicochemical factors behind their pathogenic behavior. Results revealed that gamma radiations have an extreme potential to mutate P. melonis spores, and can be a safe alternative of chemical...

Graft failure (GF) is an intractable complication of transplantation, which can severely affect the efficacy of the graft; however, the characteristics, incidence, and risk factors of primary GF have not been well described. This study aimed to analyze the risk factors and outcomes of primary GF to swiftly identify high-risk patients for GF. We performed a case-control study with a case-control ratio of 1:4 with 869 patients who underwent allogeneic hematopoietic stem cell transplantation (allo-HSCT)...