The dissipation and residues of tebuconazole in green onion (welsh onion and chive) in China were firstly investigated based on a quick, easy, cheap, effective, rugged and safe method (QuEChERS) combined with high-performance liquid chromatography-ultraviolet detector (HPLC-UV). In the method validation, the fortified recoveries ranged from 96% to 100% with relative standard deviations of ≤10%. The dissipation rate of tebuconazole follows the first-order kinetics, with the half-lives of 9.4–9.9 days. Based on the terminal residue...

In this work, a novel xonotlite nanocomposite was synthesised with abandoned eggshell by hydrothermal method, and organic-modified with toluene diisocyanate (TDI) for the removal of Pb(II) and Cd(II) from wastewater. The xonotlite and organic-modified xonotlite were characterised by X-ray diffraction (XRD), scanning electron microscopy (SEM), Brunner-Emmet-Teller (BET) and Fourier transform infrared spectroscopy (FTIR) methods. The effect of solution pH, contact time, adsorbent dose, and initial concentrations were also investigated. The organic-modified xonotlite was found to...

Different primers/probes sets have been developed all over the world for the nucleic acid detection of SARS-CoV-2 by quantitative real time polymerase chain reaction (qRT-PCR) as a standard method. In our recent study, we explored the feasibility of droplet digital PCR (ddPCR) for clinical SARS-CoV-2 nucleic acid detection compared with qRT-PCR using the same primer/probe sets issued by Chinese Center for Disease Control and Prevention (CDC) targeting viral ORF1ab or N gene, which showed that...

Quantitative real time PCR (RT-PCR) is widely used as the gold standard for clinical detection of SARS-CoV-2. However, due to the low viral load specimens and the limitations of RT-PCR, significant numbers of false negative reports are inevitable, which results in failure to timely diagnose, cut off transmission, and assess discharge criteria. To improve this situation, an optimized droplet digital PCR (ddPCR) was used for detection of SARS-CoV-2, which showed that the limit of detection...

Background: Novel coronavirus pneumonia (COVID-19) is prevalent around the world. We aimed to describe epidemiological features and clinical course in Shanghai. Methods: We retrospectively analysed 325 cases admitted at Shanghai Public Health Clinical Center, between January 20 and February 29, 2020. Results: 47.4% (154/325) had visited Wuhan within 2 weeks of illness onset. 57.2% occurred in 67 clusters; 40% were situated within 53 family clusters. 83.7% developed fever during the disease course. Median times from...

The global pandemic of coronavirus disease 2019 (COVID-19) is a disaster for human society. A convenient and reliable neutralization assay is very important for the development of vaccines and novel drugs. In this study, a G protein-deficient vesicular stomatitis virus (VSVdG) bearing a truncated spike protein (S with C-terminal 18 amino acid truncation) was compared to that bearing the full-length spike protein of SARS-CoV-2 and showed much higher efficiency. A neutralization assay was established based...

The current coronavirus disease 2019 (COVID-19) pandemic was the result of the rapid transmission of a highly pathogenic coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), for which there is no efficacious vaccine or therapeutic. Toward the development of a vaccine, here we expressed and evaluated as potential candidates four versions of the spike (S) protein using an insect cell expression system: receptor binding domain (RBD), S1 subunit, the wild-type S ectodomain (S-WT), and the...

The current coronavirus disease 2019 (COVID-19) pandemic was the result of the rapid transmission of a highly pathogenic coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), for which there is no efficacious vaccine or therapeutic. Toward the development of a vaccine, here we expressed and evaluated as potential candidates four versions of the spike (S) protein using an insect cell expression system: receptor binding domain (RBD), S1 subunit, the wild-type S ectodomain (S-WT), and the...

Co-occurrence of hypervirulence and KPC-2 carbapenem resistant phenotypes in a highly-transmissible ST11 clone ofKlebsiella pneumoniae has elicited deep concerns from public health stand point. To address this puzzle, we conducted a large-scale epidemiological, clinical and genomic study of K. pneumonia ST11 clones with both hypervirulence and carbapenem resistance in two tertiary hospitals in Zhejiang province. Most of the patients (15/23) were diagnosed with exclusively carbapenem-resistant K. pneumoniae (CRKP) infections. Ten death cases were reported, some...

An enclosed air-lift photobioreactor (ALPBR) is considered an efficient lab-scale bioreactor for microalgae cell growth and lipid biosynthesis. However, fluid-induced shear stress and mixing are two main factors that affect physiological metabolism in microalgal cell cultures. Herein, a 50-L ALPBR after being designed and manufactured was evaluated for microalgal suspension culture. Moreover, computational fluid dynamics (CFD) simulation was used to characterize the hydrodynamics of ALPBR. Specifically, two model microalgae species Chlorella vulgaris and Chlorella protothecoides...

Vip3Aa is an insecticidal protein that can effectively control certain lepidopteran pests and has been used widely in biological control. However, the mechanism of action of Vip3Aa is unclear. In the present study, we showed that Vip3Aa could cause autophagy in Sf9 cells, which was confirmed by the increased numbers of GFP-Atg8 puncta, the appearance of autophagic vacuoles, and an elevated Atg8-II protein level. Moreover, we found that the AMPK-mTOR-ULK1 pathway is involved in Vip3Aa-induced...

Supplemental Material, sj-pdf-1-ssc-10.1177_08944393211042575 for Strategic Framing Matters But Varies: A Structural Topic Modeling Approach to Analyzing China’s Foreign Propaganda About the 2019 Hong Kong Protests on Twitter by Maggie Mengqing Zhang, Xiao Wang and Yang Hu in Social Science Computer Review

Additional file 2.

This work studied the physical properties of three deep eutectic solvents (DESs) and their catalytic activity in the esterification of palmitic acid and ethanol. Three types of deep eutectic solvents (DESs) were successfully prepared using p-toluene sulfonic acid monohydrate (PTSA) as hydrogen bond donor (HBD) with dodecyl trimethyl ammonium chloride (DTAC), tetradecyl trimethyl ammonium chloride (TTAC) and octadecyl trimethyl ammonium chloride (OTAC) as hydrogen bond acceptor (HBA). The freezing points of DESs were measured in...

Skin of Niagara grapes is thin and light green, but browning and postharvest disease seriously shorten their storage life after harvest. In this study, we studied the effects of 1.0 μL L−1 1-methylcyclopropene (1-MCP) combined with 200 μL L−1 SO2 on the grape quality of Niagara grapes. It was stored in cold storage for 6 days after cold storage at low temperature and modified atmosphere packaging. 1-MCP combined with SO2 can delay the consumption of...

Skin of Niagara grapes is thin and light green, but browning and postharvest disease seriously shorten their storage life after harvest. In this study, we studied the effects of 1.0 μL L−1 1-methylcyclopropene (1-MCP) combined with 200 μL L−1 SO2 on the grape quality of Niagara grapes. It was stored in cold storage for 6 days after cold storage at low temperature and modified atmosphere packaging. 1-MCP combined with SO2 can delay the consumption of...

In the present study, we aimed to investigate the role of long non-coding RNA terminal differentiation-induced non-coding RNA (TINCR) in cisplatin (DDP) resistance of choroidal melanoma (CM) and the potential molecular mechanisms. CM and non-CM tissues were collected from 60 CM patients. DDP-resistant CM cells were obtained by selection with linearly increased DDP treatment. The expression levels of TINCR, microR-19b-3p (miR-19b-3p), and extracellular signal-regulated kinase 2 (ERK-2) were detected by quantitative real-time PCR. Cholecystokinin octapeptide...

Graft failure (GF) is an intractable complication of transplantation, which can severely affect the efficacy of the graft; however, the characteristics, incidence, and risk factors of primary GF have not been well described. This study aimed to analyze the risk factors and outcomes of primary GF to swiftly identify high-risk patients for GF. We performed a case-control study with a case-control ratio of 1:4 with 869 patients who underwent allogeneic hematopoietic stem cell transplantation (allo-HSCT)...

Macroautophagy/autophagy is a conserved cellular process associated with tumorigenesis and aggressiveness, while mechanisms regulating expression of autophagic machinery genes in cancers still remain elusive. Herein, we identified E2F4 (E2F transcription factor 4) as a novel transcriptional activator of cytoprotective autophagy crucial for zinc homeostasis in cancer cells. Gain- and loss-of-function studies showed that E2F4 promoted autophagy in a cell cycle-dependent manner, resulting in facilitated degradation of MT (metallothionein) proteins, elevated distribution of Zn2+ within autophagosomes,...

Macroautophagy/autophagy is a conserved cellular process associated with tumorigenesis and aggressiveness, while mechanisms regulating expression of autophagic machinery genes in cancers still remain elusive. Herein, we identified E2F4 (E2F transcription factor 4) as a novel transcriptional activator of cytoprotective autophagy crucial for zinc homeostasis in cancer cells. Gain- and loss-of-function studies showed that E2F4 promoted autophagy in a cell cycle-dependent manner, resulting in facilitated degradation of MT (metallothionein) proteins, elevated distribution of Zn2+ within autophagosomes,...

We have designed and prepared four new cellulose cross-linking agents using NaClO/NaBr/TEMPO and sodium periodate selective catalytic oxidation systems. First, the primary hydroxyl group of sucrose, trehalose, maltose, or lactose was carboxylated through NaClO/NaBr/TEMPO system, and then sodium periodate was used to selectively oxidize the carboxyl compound to obtain carboxyl polyaldehyde sugars. They contained multiple aldehyde groups and carboxyl groups in the molecule, had high reactivity, and can be covalently cross-linked with cellulose. To evaluate...

Increase in air pollution caused by acidic gases, such as nitrogen dioxide, call for the need to study potential effects on the structure of textile materials. Wool is a natural protein fiber which is easily damaged due to rich reaction positions and cross-linked bonds in the fiber. This study reports, for the first time, effect of nitrogen dioxide, as a model acid gas pollutant, on the structure of wool. On the macro level, wool fabric...

The demonstration of batch-to-batch consistency is indispensable for quality control of vaccines. We conducted a randomized, double-blind, parallel-controlled trial to evaluate the immunogenicity consistency of a single shot of Ad5-nCoV in healthy adults who had not previously received any COVID-19 vaccine. All eligible participants were randomly assigned equally to receive one of the three consecutive batches of Ad5-nCoV (5 × 1010 viral particles/vial, 0.5 mL). The primary endpoint was geometric mean titers (GMTs) of serum...

Corporate probability of default (PD) prediction is vitally important for risk management and asset pricing. In search of accurate PD prediction, we propose a flexible yet easy-to-interpret default-prediction single-index hazard model (DSI). By applying it to a comprehensive U.S. corporate bankruptcy database we constructed, we discover an interesting V-shaped relationship, indicating a violation of the common linear hazard specification. Most importantly, the single-index hazard model passes the Hosmer-Lemeshow goodness-of-fit calibration test while neither does a...

The reduction of CO2 into valuable chemicals through electrocatalytic techniques provides a potential strategy to alleviate energy crises and environmental pollution. In this study, 2D M3(THT)2 (M = Fe, Co, Ni, Ru, Rh, Pd, THT = 2,3,6,7,10,11-triphenylenehexathiolate) framework as CO2 reduction reaction (CO2RR) electrocatalysts was investigated by using the density functional method. The results showed that the studied catalysts are stable both thermodynamically and electrochemically. Rh3(THT)2 exhibits the best catalytic performance to produce CH4 with...