Additional file 1. A Body weight of CIA rats. B Arthritis index of CIA rats (* P-value < 0.05).

Additional file 1. All original and unprocessed images of Western blots.

SARS-CoV-2 variants continue to emerge facing established herd immunity. L452R, previously featured in the Delta variant, quickly emerged in Omicron subvariants, including BA.4/BA.5, implying a continued selection pressure on this residue. The underlying links between spike mutations and their selective pressures remain incompletely understood. Here, by analyzing 221 structurally characterized antibodies, we found that IGHV1-69-encoded antibodies preferentially contact L452 using germline-encoded hydrophobic residues at the tip of HCDR2 loop. Whereas somatic hypermutations or VDJ rearrangements...

Additional file 2: Table S2. Univariable Cox regression analysis for variables and long-term all-cause mortality.

Supplementary Material

It is a decade-long controversy that transarterial chemoembolization (TACE) has definite priority over transarterial embolization (TAE) in treating patients with hepatocellular carcinoma (HCC), since HCC cells are regularly resistant to chemotherapy by enhanced expression of proteins that confer drug resistance, and ABC transporters pump the intracellular drug out of the cell. We addressed this issue by modulating the chemo-environment. In an animal model, sevelamer, a polymeric phosphate binder, was introduced as an embolic agent to...

In this paper, we investigated the electro-optical properties of the dilute magnetic polymer dispersed liquid crystals (PDLCs) prepared with the liquid crystal colloids containing low concentrations of γ-Fe2O3 nanoparticles. The doped nanoparticles have hardly changed the morphology of PDLCs, but they have significantly changed the threshold voltage, saturation voltage, and contrast ratio of PDLCs. Such changes result from ionic adsorption in competition with the concentration of the nanoparticles. When the concentration of the nanoparticles was...

In this paper, we investigated the electro-optical properties of the dilute magnetic polymer dispersed liquid crystals (PDLCs) prepared with the liquid crystal colloids containing low concentrations of γ-Fe2O3 nanoparticles. The doped nanoparticles have hardly changed the morphology of PDLCs, but they have significantly changed the threshold voltage, saturation voltage, and contrast ratio of PDLCs. Such changes result from ionic adsorption in competition with the concentration of the nanoparticles. When the concentration of the nanoparticles was...

The Mesozoic-Cenozoic geological evolution of the southern Great Xing’an Range (GXR) has long been controversial since it is located in the superposed region of different tectonic regimes and involves complex interactions. Here, we present low-temperature thermochronological data from granitoids emplaced in multiple magmatic stages to delineate the tectono-thermal history and further understand the geodynamic process. Most zircon and apatite (U-Th)/He ages, within error, indicate Early Cretaceous: the zircon average (U-Th)/He ages range from 146.7 ±...

The oncogenic role of actin filament-associated protein 1 antisense RNA 1 (AFAP1-AS1) has been reported in retinoblastoma (RB). However, the underlying regulatory mechanisms remain poorly understood. In this study, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and Western blotting were performed to analyze the expression of AFAP1-AS1, microRNA miR-545-3p, or G protein subunit beta 1 (GNB1). Cell Counting Kit-8 (CCK-8) and Transwell migration assays were used to detect cell proliferation and migration. In addition, caspase-3 activity...

The present work aims to prove the concept of tumor-targeted drug delivery mediated by platelets. Doxorubicin (DOX) attached to nanodiamonds (ND-DOX) was investigated as the model payload drug of platelets. In vitro experiments first showed that ND-DOX could be loaded in mouse platelets in a dose-dependent manner with a markedly higher efficiency and capacity than free DOX. ND-DOX-loaded platelets (Plt@ND-DOX) maintained viability and ND-DOX could be stably held in the platelets for at least 4...

Pangolins have gained increasing global attention owing to their public health significance as potential zoonotic hosts since the identification of SARS-CoV-2-related viruses in them. Moreover, these animals could carry other respiratory viruses. In this study, we investigated the virome composition of 16 pangolins that died in 2018 with symptoms of pneumonia using metagenomic approaches. A total of eight whole virus sequences belonging to the Paramyxoviridae or Pneumoviridae families were identified, including one human parainfluenza virus...

Additional file 1. Original images for western blot.

Additional file 1: Figure S1. The relationship between a healthy lifestyle and CMM in subgroup analyses. Figure S2. The relationship between a healthy lifestyle and CMM in seven sensitivity analyses. Figure S3. The frequency distribution graph of the weighted healthy lifestyle score for the whole population. Figure S4. The frequency distribution graph of the weighted healthy lifestyle score for 2/3 of the population. Figure S5. The frequency distribution graph of the sum healthy lifestyle score...

Supplementary Material 1

Additional file 5. Original images of Western blots in Fig. 2D, Fig. 3D, Fig. 3E, Fig. 3G, and Fig. 4B, accompanied with brief instructions.

Additional file 7: Fig. S4. DNA methylation profiles of protein-coding genes (PCgenes) in sunflower endosperm from SY1.

Additional file 1: Table S1. The hepatic manifestations of the patient (Family-1-II-2) carrying TTC21B mutations at the age of 2 months

Additional file 1.

Additional file 1. Supporting information.

Additional file 1. Supporting information.

Additional file 2. The NC3Rs ARRIVE guidelines 2.0 author checklist for reporting animal research.

Additional file 2. The NC3Rs ARRIVE guidelines 2.0 author checklist for reporting animal research.

Additional file 1.

Additional file 2: Figure S1. Time-kill kinetics of polymyxin B (PolyB; 1 mg/L), rifampicin (Rif; 2 mg/L), and their combination against P. aeruginosa PAO1 at starting 600 nm (OD600) of ~ 0.5 (~ 108 CFU/mL). Figure S2. GO enrichment analysis of the significantly changed genes with the polymyxin B/rifampicin combination at (A) 1 h and (B) 24 h: (i) downregulation and (ii) upregulation of various biological processes. Figure S3. Overview of metabolic pathways of P....