The adsorption of phages to hosts is the first step of phage infection. Studies have shown that tailed phages use tail fibres or spikes to recognize bacterial receptors and mediate adsorption. However, whether other phage tail components can also recognize host receptors is unknown. To identify potential receptors, we screened a transposon mutagenesis library of the marine pathogen Vibrio parahaemolyticus and discovered that a vp0980 mutant (vp0980 encodes a predicted transmembrane protein) could not be...

The present study aimed to assess the role of miR-1275 in cardiac ischemia reperfusion injury. H9 human embryonic stem cell (hESC)-derived cardiomyocytes stimulated by oxygen-glucose deprivation/reoxygenation (OGD/R) were used to simulate myocardial injury in vitro. miR-1275 expression levels in cells were measured by RT-qPCR. The release of lactate dehydrogenase (LDH) and creatine kinase (CK) was examined through LDH and CK ELISA kits. Cell apoptosis was detected through flow cytometry. A Fura-2 Calcium Flux Assay Kit...

Co-occurrence of hypervirulence and KPC-2 carbapenem resistant phenotypes in a highly-transmissible ST11 clone ofKlebsiella pneumoniae has elicited deep concerns from public health stand point. To address this puzzle, we conducted a large-scale epidemiological, clinical and genomic study of K. pneumonia ST11 clones with both hypervirulence and carbapenem resistance in two tertiary hospitals in Zhejiang province. Most of the patients (15/23) were diagnosed with exclusively carbapenem-resistant K. pneumoniae (CRKP) infections. Ten death cases were reported, some...

COVID-19 is caused by SARS-CoV-2 infection and was initially discovered in Wuhan. This outbreak quickly spread all over China and then to more than 20 other countries. SARS-CoV-2 fluorescent microsphere immunochromatographic test strips were prepared by the combination of time-resolved fluorescence immunoassay with a lateral flow assay. The analytical performance and clinical evaluation of this testing method was done and the clinical significance of the testing method was verified. The LLOD of SARS-CoV-2 antibody IgG...

Supplemental material, sj-pdf-2-ppa-10.1177_09520767211034664 for The evolution of collaborative networks: A social network analysis of Chinese environmental protection policy by Yanwei Li and Jing Huang in Public Policy and Administration

Cyclin-dependent kinase 9 (CDK9) phosphorylates RNA polymerase II to promote productive transcription elongation. Here we show that short-term CDK9 inhibition affects the splicing of thousands of mRNAs. CDK9 inhibition impairs global splicing and there is no evidence for a coordinated response between the alternative splicing and the overall transcriptome. Alternative splicing is a feature of aggressive prostate cancer (CRPC) and enables the generation of the anti-androgen resistant version of the ligand-independent androgen receptor, AR-v7. We...

Aminoglycosides exhibit ototoxicity by damaging mitochondria, which in turn generate reactive oxygen species that induce hair cell death and subsequent hearing loss. It is well known that damaged mitochondria are degraded by mitophagy, an important mitochondrial quality control system that maintains mitochondrial homeostasis and ensures cell survival. However, it is unclear whether dysregulation of mitophagy contributes to aminoglycoside-induced hair cell injury. In the current study, we found that PINK1-PRKN-mediated mitophagy was impaired in neomycin-treated hair...

Phosphorus (P) as an essential nutrient for life sustains the productivity of food systems; yet misdirected P often accumulates in wastewater and triggers water eutrophication if not properly treated. Although technologies have been developed to remove P, little attention has been paid to the recovery of P from wastewater. This work provides a comprehensive review of the state-of-the-art P removal technologies in the science of wastewater treatment. Our analyses focus on the mechanisms, removal efficiencies,...

Additional file 1: Table S1. Search strategy of journal publications on HTA of selected medical devices. Table S2. Search strategy of journal publications on discussing HTA of medical devices. Figure S1. PRISMA flowchart of literature review of journal publications on discussing HTA of medical devices.

The programmed death ligand 1 (PD-L1) is a pivotal biomarker of immunotherapy in triple negative breast cancer (TNBC). TP53 is reported as a positive regulatory predictor of immune efficacy. The correlation of p53 expression or mutation and PD-L1 expression is explored. By immunohistochemistry, PD-L1 expression between p53 mutation (missense and nonsense) and wild type; p53 no-expression/loss vs. expression were compared. There was a significant association between p53 mutation, especially missense mutation with higher histological grade,...

Studies have shown the inhibitory effect of microRNA-34a on proliferation, migration, and invasion of oral squamous cell carcinoma. However, the lack of a safe and effective delivery system limits the clinical application of microRNA-34a in oral cancer treatment. An exosome is a small extracellular vesicle that mediates intercellular communication by delivering proteins, nucleic acids, and other contents, and functions as a natural drug delivery carrier. Here, we aimed to explore whether exosomes could be used...

Drug delivery and triggered release in tumor cells would realize the ultimate goal of precise cancer treatment. An APE1 triggered DNA nanoprism was designed, aiming at the applications of both drug delivery and precise triggered drug release in cancer cell. We demonstrate that the AP-Prism was successfully used as a vehicle based on the intracellular endogenous enzyme APE1 triggered for controlled drug delivery and triggered release. The box like DNA prism was self-assembled by annealing...

Additional file 2. Supplementary Figures.

Additional file 8: Appendix 7. Grading of Recommendations, Assessment, Development and Evaluations (GRADE).

Additional file 1: Appendix 1. PRISMA Checklist. Appendix 2. Protocol. Appendix 3. Search Strategy. Appendix 4. Outcomes. Appendix 5. Definitions of exercise types and non-exercise training control. Appendix 6. Statistical methods in details. Appendix 7. Assessment of the transitivity. Appendix 8. Characteristics of studies and subjects included in the review. Appendix 9. Risk of Bias. Appendix 10. Results from network meta-analyses. Appendix 11. Evaluation of heterogeneity and inconsistency. Appendix 12. Publication bias. Appendix 13. Network...

Additional file 1: Table S1. Search strategy.

Additional file 3: Fig. S1. Risk of bias summary. Fig. S2. Risk of bias graph for each included study. Fig. S3. Sensitivity analyses for PFS. Fig. S4. Sensitivity analyses for OS. Fig. S5. Meta-regression for OS by maintenance dose. Fig. S6. Funnel plots for PFS and OS.

Foeniculins A-C (1–3) together with a pair of enantiomers (±)-foeniculin D (4) were isolated from endophytic fungus Diaporthe foeniculina BZM-15. Their structures including absolute configurations were unambiguously established by extensive interpretation of the NMR and HR-ESI-MS data, ECD measurements powered by molecular calculations, as well as Mo2(OAc)4 mediated CD methodology. The cytotoxic activity assay disclosed that these compounds didn’t show any noticeable cytotoxic activity.

Supplemental material, sj-docx-1-oed-10.1177_25158414221139002 for A medication-wide association study to identify medications associated with incident clinically significant diabetic retinopathy by Ruilin Xiong, Wei Wang, Xianwen Shang, Yixiong Yuan, Yifan Chen, Lei Zhang, Katerina V Kiburg, Zhuoting Zhu and Mingguang He in Therapeutic Advances in Ophthalmology

The present study was aimed to evaluate the expression profile of Zinc finger C3H1 domain-containing protein (ZFC3H1) using bioinformatic analysis of public datasets from The Cancer Genome Atlas database (TCGA). The results showed that the expression levels of ZFC3H1 were notably lower than the corresponding non-cancerous tissues in prostate adenocarcinoma (PRAD), and patients in the high ZFC3H1-expression group showed poor survival. We hypothesized that the low expression of ZFC3H1 in tumor tissue might have be...

Supplemental material, sj-docx-1-tan-10.1177_17562864231154976 for Short-term outcome prediction for myasthenia gravis: an explainable machine learning model by Huahua Zhong, Zhe Ruan, Chong Yan, Zhiguo Lv, Xueying Zheng, Li-Ying Goh, Jianying Xi, Jie Song, Lijun Luo, Lan Chu, Song Tan, Chao Zhang, Bitao Bu, Yuwei Da, Ruisheng Duan, Huan Yang, Sushan Luo, Ting Chang and Chongbo Zhao in Therapeutic Advances in Neurological Disorders

Spontaneous imbibition is one of the important means to enhance oil recovery in low permeability reservoirs, and it has good field application effects. Through the interfacial tension (IFT), contact angle (CA), and adsorption experiments, the effects of SDBS surfactant on IFT and reservoir wettability were studied, and the adsorption rule of SDBS at the mineral surface was obtained. On this basis, the imbibition recovery and mechanism of cores with different permeability are studied. The study...

This supplemental document describes details of the optimum relationship between etalon angle, periscope angle, cavity length, and periscope length, and details of the maximum MHF tuning range for the designed periscope.

Psoriasis is a chronic inflammatory skin disease. Mast cells are significantly increased and activated in psoriatic lesions and are involved in psoriatic inflammation. Some endogenous substances can interact with the surface receptors of mast cells and initiate the release of downstream cytokines that participate in inflammatory reactions. Neuroblast differentiation-associated protein (AHNAK) is mainly expressed in the skin, esophagus, kidney, and other organs and participates in various biological processes in the human body. AHNAK and its...

Additional file 1. Supplementary figures, materials, and methods.