Soil salinization significantly restricts crop yield, animal foraging, and biomass production for biofuels. Identifying an efficient farming system to mitigate these concerns has become a major research focus. The present study addresses the potential of rotating triticale and sweet sorghum at various planting densities to alleviate soil salt stress, enhance production, improve agronomic characteristics, and boost soil microbial communities in a saline agricultural environment in East China. Several indicators in 2017 and 2018. Results show...

Additional file 2. Supplementary methods and materials.

Additional file 3. Primers of target genes.

Additional file 3. Primers of target genes.

Additional file 1.Table S1. Primers Sequences used in q-PCR assay.

Additional file 1: Additional Figure 1. Study design and study timeline. AE, adverse event; OHSS, ovarian hyperstimulation syndrome; GnRH, gonadotropin-releasing hormone

Additional file 1. Other details of this study.

The establishment of structure activity relationship (SAR) for rakicidin derivatives is pretty vital to develop rakicidins as a new type of anti-cancer agents. Herein, two novel rakicidin derivatives, compounds B1-1 (1) and B1-2 (2), a cyclic depsipeptide and a chain lipopeptide, respectively, were isolated from culture broth of Micromonospora chalcea FIM-R160609, and their structures were elucidated clearly by extensive NMR and HR-ESI-MS analyses. Following, their cytotoxic activities were evaluated against HCT-8 and PANC-1 human cancer...

Additional file 1:Supplemental Table 1. Ramsay sedation scale. Supplemental Table 2. American Academy of Sleep Medicine (AASM) sleep score.

In this paper, we present a Markov chain Monte Carlo (MCMC) simulation algorithm for estimating parameters in the kernel density estimation of bivariate insurance claim data via transformations. Our data set consists of two types of auto insurance claim costs and exhibit a high-level of skewness in the marginal empirical distributions. Therefore, the kernel density estimator based on original data does not perform well. However, the density of the original data can be estimated through...

Haloacetic acids (HAAs) are a class of disinfection by-products (DBPs), widely occurring in drinking water, wastewater and swimming pool water with concentrations at sub-μg/L to mid-μg/L levels. HAAs arose public attention due to their cytotoxicity, genotoxicity and carcinogenicity. Monitoring HAA concentration levels in aqueous environments is required. This review presents an overview of all techniques or methods for sample pretreatment, analysis and removal of HAAs in aqueous environments since 2000. For the pretreatment methods, the...

Additional file 1. Figure S1: Mendelian error rate of all families. Figure S2: The number of de novo mutations in probands and siblings followed Poisson distribution. Figure S3: Gene (protein synthesis) expression in different brain regions at various periods. Figure S4: Gene (energy) expression in different brain regions at various periods. Figure S5: Gene (pressure) expression in different brain regions at various periods. Figure S6: Gene (amino acid) expression in different brain regions at various...

Although various dual-target tubulin inhibitors have been designed and synthesised, no dual tubulin-NEDDylation inhibitors as antiproliferative agents were reported so far. In this work, a series of trimethoxyphenyl analogues as potential dual tubulin-NEDDylation inhibitors were synthesised and evaluated for their antiproliferative activity. Among them, compound C11 exhibited the most potent inhibitory activity with IC50 values of 1.17, 2.48, and 1.47 μM against HepG2, PC3, and MCF7 cells, respectively. In addition, it displayed the potent inhibitory...

The present work aims to prove the concept of tumor-targeted drug delivery mediated by platelets. Doxorubicin (DOX) attached to nanodiamonds (ND-DOX) was investigated as the model payload drug of platelets. In vitro experiments first showed that ND-DOX could be loaded in mouse platelets in a dose-dependent manner with a markedly higher efficiency and capacity than free DOX. ND-DOX-loaded platelets (Plt@ND-DOX) maintained viability and ND-DOX could be stably held in the platelets for at least 4...

Radiofrequency ablation (RFA) and microwave ablation (MWA) are widely used in combination with transarterial chemoembolization (TACE) for intermediate-stage hepatocellular carcinoma (HCC) in clinical practice. We aim to compare the efficacy and safety of TACE combined with RFA or MWA versus TACE monotherapy for intermediate-stage HCC. We searched PubMed, Embase, Web of Science, and the Cochrane Library for relevant studies. The outcomes included overall survival (OS), progression-free survival (PFS), local tumor control (LTC) rate, and major...

Copper diethyldithiocarbamate (Cu(DDC)2) has been demonstrated to possess excellent antitumor activity. However, the extremely poor water solubility of Cu(DDC)2 bring difficulty for its formulation research. In this study, we aim to develop a novel nanocarrier for Cu(DDC)2 delivery to overcome this obstacle and enhance antitumor activity. The SP94 modified asymmetrical bilayer lipid-encapsulated Cu(DDC)2 nanoparticles (DCDP) was established by combining the method of inverse microemulsion aggregation and thin-film dispersion. In vitro cellular assays and in vivo...

To date, several different types of synthetic genetic switches, including riboregulators, riboswitches, and toehold switches, have been developed to construct AND, OR, NOT, NAND, NOR, and NOT IMPLICATION (NIMP) gates. The logic gate can integrate multiple input signals following a set of algorithms and generate a response only if strictly defined conditions are met. However, there are still some logic gates that have not been implemented but are necessary to build complex genetic circuits. Here,...

Clear cell renal cell carcinoma, the most common type of renal cancer, is associated with poor survival. Ubiquitin-specific peptidase 2 regulates the molecular mechanisms of cancer cells. However, its mechanism in clear cell renal cell carcinoma remains unclear. Quantitative real-time polymerase chain reaction, enzyme-linked immunosorbent assay, and immunohistochemistry were performed to assess ubiquitin-specific peptidase 2 expression in human clear cell renal cell carcinoma samples. Ubiquitin-specific peptidase 2 was weakly expressed in clear cell renal cell...

Sponge iron (SI) is widely used in water treatment. As effluents from wastewater treatment plant (WWTP) require advanced treatment methodology, three forms of constructed wetlands (CWs): wetlands with sponge iron (SI), copper sulfate modified sponge iron (Cu/SI), and sponge iron coupled with solid carbon sources (C/SI), have been investigated in this paper for the removal effects of organic matter and nutrients in WWTP effluents, and the corresponding mechanisms have been analyzed. The results showed the...

Additional file 2: Figure S1. Original blot and images. Figure S2. HMGB2 expression is not correlated with survival of patients with LUSC.

Additional file 7: Table S2. The primer sequences used in the study.

Additional file 3: Fig. S3. The time dependent ROC curves for other three prognostic models. A Time dependent ROC curves for immune-related gene signature in the TCGA GBM cohort. B Time dependent ROC curves for pyroptosis-related gene signature in the TCGA GBM cohort. C Time dependent ROC curves for autophagy-related gene signature in the TCGA GBM cohort.

Additional file 4: Fig. S4. Validation of independence of our risk score as a prognostic factor. A The forest plot showed the univariate cox analysis using risk score, age, radiotherapy, TMZ chemotherapy, and gender as variates in the GSE16011 GBM cohort. B The forest plot showed the multivariate cox analysis using risk score, age, radiotherapy, and TMZ chemotherapy as variates in the GSE16011 GBM cohort. C Stratified OS analysis in GSE16011 GBM patients with different...

Additional file 4. miRNA sequences.

Additional file 5. Coding Sequence of Irak1 and Traf6 Genes.