Radiofrequency ablation (RFA) and microwave ablation (MWA) are widely used in combination with transarterial chemoembolization (TACE) for intermediate-stage hepatocellular carcinoma (HCC) in clinical practice. We aim to compare the efficacy and safety of TACE combined with RFA or MWA versus TACE monotherapy for intermediate-stage HCC. We searched PubMed, Embase, Web of Science, and the Cochrane Library for relevant studies. The outcomes included overall survival (OS), progression-free survival (PFS), local tumor control (LTC) rate, and major...

Additional file 1: Table S1. Production of SFAs and UFAs in wild type Escherichia coli Rosetta overexpressing EhDH domains. Table S2. Production of SFAs and UFAs in wild-type Escherichia coli Rosetta overexpressing the mutated EhDH domains. Table S3. Primers used for DH domain amplifications. Table S4. Primers used for site-directed mutagenesis of DH domains. Table S5. Primers used for plant experiment. Figure S1. Expression of recombinant proteins when induced with 0.2 mM IPTG in Escherichia...

Additional file 1: Fig.S1. A PCR amplification of α-L-rhamnosidase gene from 11 positive clones (N1, N4, N5, N6, N8, N10, N12, N13,N14, N24 and N28). B SDS-PAGE analysis of N-glycosidase F treated N12-Rha.Lane 1, untreated N12-Rha; lane 2, N12-Rha treated with N-Glycosidase F. Fig.S2. Ramachandran plot A and Verify-3D B of N12-Rha model

Additional file 1. Fig. S1. Resting-state network components selected in AD patients. Fig. S2. Selected seeds in the different resting-state networks.

The effective electrocatalysts for hydrogen evolution reaction (HER) in alkaline aqueous is critical point to reduce energy losses for water electrolysis. Here, a highly active and stable electrochemical catalyst of platinum (Pt) nanoparticles loaded on three-dimensional Ti3C2Tx-reduced graphite oxide (rGO) hybrid support (Pt/Ti3C2Tx-rGO3D) has been developed for hydrogen evolution reaction (HER). The large surface of 3 D support provides an adequate site for deposition of Pt nanoparticles, leading to superior HER activity with low overpotential...

Resistance to chemotherapeutic drugs limits the control of gastric cancer (GC) development. The study intended to probe into the mechanism of aquaporin 3 (AQP3) on the chemoresistance of GC. Cisplatin (CDDP)-resistant cells were constructed. Parental AGS and HGC-27 cells and their respective CDDP-resistant cells were transfected with AQP3 overexpression plasmid, AQP3 short hairpin RNA (sh-AQP3) and sh-Kruppel-like factor 5 (shKLF5). The expressions of AQP3 and factors related to autophagy (LC3 I, LC3 II, Atg5, Beclin-1,...

Clear cell renal cell carcinoma, the most common type of renal cancer, is associated with poor survival. Ubiquitin-specific peptidase 2 regulates the molecular mechanisms of cancer cells. However, its mechanism in clear cell renal cell carcinoma remains unclear. Quantitative real-time polymerase chain reaction, enzyme-linked immunosorbent assay, and immunohistochemistry were performed to assess ubiquitin-specific peptidase 2 expression in human clear cell renal cell carcinoma samples. Ubiquitin-specific peptidase 2 was weakly expressed in clear cell renal cell...

Sponge iron (SI) is widely used in water treatment. As effluents from wastewater treatment plant (WWTP) require advanced treatment methodology, three forms of constructed wetlands (CWs): wetlands with sponge iron (SI), copper sulfate modified sponge iron (Cu/SI), and sponge iron coupled with solid carbon sources (C/SI), have been investigated in this paper for the removal effects of organic matter and nutrients in WWTP effluents, and the corresponding mechanisms have been analyzed. The results showed the...

SCFD1 (sec1 family domain containing 1) was recently shown to function in autophagosome-lysosome fusion, and multiple studies have demonstrated the regulatory impacts of acetylation (a post-translational modification) on macroautophagy/autophagy. Here, we demonstrate that both acetylation- and phosphorylation-dependent mechanisms control SCFD1ʹs function in autophagosome-lysosome fusion. After detecting a decrease in the extent of SCFD1 acetylation under autophagy-stimulated conditions, we found that KAT2B/PCAF catalyzes the acetylation of residues K126 and K515 of SCFD1; we also showed that...

Additional file 2: Figure S1. Original blot and images. Figure S2. HMGB2 expression is not correlated with survival of patients with LUSC.

In this study, blueberry fruits (Vaccinium corymbosum cv. ‘Bluerain’) were sprayed with different concentrations (0.25, 0.5, 0.75, and 1 mg/L) of exogenous 24-epibrassionolide (EBR) after harvest and kept at 18°C and relative humidity of 90∼95% for 8 d. By observing the decay incidence and firmness changes, the optimal treatment concentration (0.75 mg/L) was screened, and then the effects of 0.75 mg/L EBR treatment on fruit quality and antioxidant activities were further explored. The results showed...

Additional file 2. Supplementary methods and materials.

Supplemental Material for The impact of comprehensive blood conservation program on major complications after total aortic arch replacement by Weidong Yan, Qiaoni Zhang, Sizhe Gao, Gang Liu, Yuan Teng, Jing Wang, Jian Wang, Boyi Zhou, Shujie Yan, and Bingyang Ji in Perfusion

Supplemental Material for The impact of comprehensive blood conservation program on major complications after total aortic arch replacement by Weidong Yan, Qiaoni Zhang, Sizhe Gao, Gang Liu, Yuan Teng, Jing Wang, Jian Wang, Boyi Zhou, Shujie Yan, and Bingyang Ji in Perfusion

Supplement 1

Additional file 1: Supplement Table 1. Supplement Table 2. Comparison of Complication Rates in Patients with Inflammatory Arthritis and Control Patients with Osteoarthritis. Supplement Table 3. Comparison of Complication Rates Among Patients with Different types of inflammatory arthritis. Supplement Table 4. Comparison of Complication Rates in Patients in each group between emergency or elective hospitalizations.

Ferromanganese nodules are a potential energy resource because of their high contents of economically interesting elements (i.e. Mn, Ni, Cu, and Zn). These are higher in diagenetic layers than in hydrogenetic layers. The study of the causes of elemental accumulation in the diagenetic layer is useful for the exploration metal-rich nodules. A diagenetic-dominant ferromanganese nodule, from the central Clarion–Clipperton Fracture Zone of the eastern Pacific Ocean was studied from core to rim. It was divided...

Additional file 1: Fig. S1. Validation of DE-MRGs related prognostic risk model for GBM. A, E Time dependent ROC curves for 12 DE-MRGS prognostic model in the GSE147352 and GSE16011 GBM cohort. B, F The distribution of risk scores, survival time, and status of GBM patients in the GSE147352 and GSE16011 GBM cohort. C, GThe heatmap of the 12 model DE-MRGs in the GSE147352 and GSE16011 GBM cohort. D, H Kaplan–Meier curves for OS in...

Additional file 1.

Supplemental material, sj-docx-1-hol-10.1177_09596836221138336 for Synchronous change in the intensified millet cultivation and ecological environment from the early to middle Holocene on the Inner Mongolia Plateau, northern China by Keliang Zhao, Huiping Wei, Zhanhu Zhao, Yaping Zhang, Wenqing Liu, Jian Wang, Guanhan Chen, Hui Shen, Hua Du, Peng Cheng, Shan Chen, Peter Weiming Jia, Xinying Zhou and Xiaoqiang Li in The Holocene

Supplemental material, sj-docx-1-hol-10.1177_09596836221138336 for Synchronous change in the intensified millet cultivation and ecological environment from the early to middle Holocene on the Inner Mongolia Plateau, northern China by Keliang Zhao, Huiping Wei, Zhanhu Zhao, Yaping Zhang, Wenqing Liu, Jian Wang, Guanhan Chen, Hui Shen, Hua Du, Peng Cheng, Shan Chen, Peter Weiming Jia, Xinying Zhou and Xiaoqiang Li in The Holocene

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The precipitation hardening is widely applied to strengthen Al alloys. Here, we report an alternative way to achieve high-strength Al alloys via forming the dislocation gradient through pre-torsion. Remarkably, the gradient Al alloys exhibit a sharp increase in ultimate tensile strength up to 605 MPa yet nearly without reducing the uniform elongation, thereby outperforming their counterparts. The superior strength–ductility combination is ascribed to the excellent strengthening and strain hardening effects stemming from the substantial hetero-deformation-induced...

A high-dimensional neural network interatomic potential was developed and used in molecular dynamics simulations of condensed phase Ni and Ni systems with liquid–solid phase coexistence. The reference data set was generated by sampling the potential energy surface over a broad temperature-pressure domain using ab initio MD simulations to train a unified potential. Excellent agreement was achieved between bulk face-centred cubic nickel thermal expansion simulations and relevant experimental data. The same potential also yields accurate structures...