Following acute infection, individuals COVID-19 may still shed SARS-CoV-2 RNA. However, limited information is available regarding the active shedding period or whether infectious virus is also shed. Here, we monitored the clinical characteristics and virological features of 38 patients with COVID-19 (long-term carriers) who recovered from the acute disease, but still shed viral RNA for over 3 months. The median carrying history of the long-term carriers was 92 days after the first admission, and the...

Following acute infection, individuals COVID-19 may still shed SARS-CoV-2 RNA. However, limited information is available regarding the active shedding period or whether infectious virus is also shed. Here, we monitored the clinical characteristics and virological features of 38 patients with COVID-19 (long-term carriers) who recovered from the acute disease, but still shed viral RNA for over 3 months. The median carrying history of the long-term carriers was 92 days after the first admission, and the...

Bladder cancer (BLCA) is a common malignancy and has a poor prognosis. Single nucleotide polymorphisms (SNPs) in genes are closely associated with the tumorigenesis and tumor development and yet are not well illustrated in BLCA. In this study, we downloaded SNP‐related data and transcriptome profiling of BLCA from the Cancer Genome Atlas (TCGA) database and identified high frequency mutation genes using Maftools package and differentially expressed genes (DEGs) between BLCA and normal bladder tissues by...

Heavy metal ion pollution has become a serious problem. In this paper, a new type of adsorbent, reduced graphene oxide grafted by 4-sulfophenylazo groups (RGOS), was synthesized to adsorb heavy metal ions in an aqueous solution via two kinds of adsorption modes, ion exchange and coordination. The maximum adsorption capacities of the RGOS for Pb(II), Cu(II), Ni(II), Cd(II) and Cr(III) were 689, 59, 66, 267 and 191 mg/g, respectively. Adsorption equilibrium time of RGOS for...

The present study explored the cooperative effect of both alanine (Ala) and gentamicin (Gent) on metabolic mechanisms by which exogenous Ala potentiates Gent to kill antibiotic-resistant Vibrio alginolyticus. To test this, GC-MS-based metabolomics was used to characterize Ala-, Gent- and both-induced metabolic profiles, identifying nitric oxide (NO) production pathway as the most key clue to understand metabolic mechanisms. Gent, Ala and both led to low, lower and lowest activity of total nitric oxide synthase (tNOS)...

To clarify the effects of mechanically dry direct-seeded rice (MD-DSR) through drilling and controlled-release compound fertilizer (CRF) on rice production, field experiments were carried out with mixed controlled-release urea; controlled-release potassium; and common urea, phosphorus and potash fertilizers as test materials. The experiment was implemented with a split-plot design, with fertilization modes as the main plots and two varieties (indica and japonica) as the subplots. The fertilization modes were a one-time application of CRF (denoted...

Bananas (Musa spp.) enriched in resistant starch displayed multiple health benefits for diabetes. Here, we showed that banana starch administered to diabetic rats improved animal depressive-like behaviours of mood disorders. Four weeks of banana starch diet to high-sugar, high-fat, and STZ-induced diabetic rats lowered their blood insulin resistance, but increased their plasma HDL cholesterol and 5-HT levels, compared to the control groups of rats. The banana starch diet reconstructed the gut microbiota and increased their...

An immunochromatographic assay (ICA) based on surface-enhanced Raman scattering (SERS) for ultrasensitive and quantitative detection of florfenicol (FF) in food samples was developed. Au nanocubers (AuNCs) with long wavelength absorption were synthesised, characterised and used as the substrate in SERS-ICA. Immunoprobe was prepared by linking Raman reporter and the antibody against FF on AuNCs. The SERS-ICA was completed in 15 min. By measuring the Raman intensities on T lines, quantitative detection of FF was achieved....

Additional file1: Figure S1. Representative immunohistochemical images of the expression of omentin1 in adipose tissues of mice injected with AAV-NC or AAV-omentin1, and the statistical results of staining intensity of omentin1 were presented, scale bar = 250 μm (n = 3). The experiments were performed in triplicate. Results were expressed as mean ± SD. ##P < 0.01, ####P < 0.0001 vs. the sham group; ****P < 0.0001 vs. the model group, one-way ANOVA. Figure S2....

Additional file 2: Includes 9 figures of additional results. The names of the figures are: Figure S1. Performance (AUC) of different methods benchmarked using the rare nsSNV test set (RNTS, test set 1); Figure S2. Performance (AUC) of different methods benchmarked using the de-novo rare ClinVar test set (DN-RCTS, test set 3); Figure S3. Performance (precision-recall curve) of different methods benchmarked using the de-novo rare ClinVar test set (DN-RCTS, test set 3); Figure S4. Performance...

Additional file 1: Appendix 1. PRISMA Checklist. Appendix 2. Protocol. Appendix 3. Search Strategy. Appendix 4. Outcomes. Appendix 5. Definitions of exercise types and non-exercise training control. Appendix 6. Statistical methods in details. Appendix 7. Assessment of the transitivity. Appendix 8. Characteristics of studies and subjects included in the review. Appendix 9. Risk of Bias. Appendix 10. Results from network meta-analyses. Appendix 11. Evaluation of heterogeneity and inconsistency. Appendix 12. Publication bias. Appendix 13. Network...

Supplemental material, sj-docx-1-mrx-10.1177_01979183221126726 for Cumulative Experiences of Immigration Enforcement Policy and the Physical and Mental Health Outcomes of Asian and Latinx Immigrants in the United States by Maria-Elena De Trinidad Young, Lei Chen, May Sudhinaraset, Altaf Saadi, Kathryn G. Kietzman, and Steven P. Wallace in International Migration Review

Additional file 1. Depression severity according to status and consumption of cigarette smoking or alcohol drinking.

Additional file 3. Odds of depression symptoms according to status and consumption of alcohol drinking stratified by age.

Supplemental material, sj-docx-1-jbm-10.1177_03936155221132572 for A study on the correlation between M2 macrophages and regulatory T cells in the progression of colorectal cancer by Yanlei Chen, Yu Gao, Xueqian Ma, Yanping Wang, Jinhao Liu, Chunyu Yang, Yue Wang, Cuifen Bao, Xiaoyu Song, Yang Feng, Yan Sun and Shifeng Qiao in The International Journal of Biological Markers

This study aimed to identify physiological and pharmacogenomic covariates and develop a population pharmacokinetic model of high-dose methotrexate (HD-MTX) in Chinese paediatric patients with acute lymphoblastic leukaemia (ALL) and malignant lymphoma.A total of 731 MTX courses and 1658 MTX plasm concentrations from 205 paediatric patients with ALL and malignant lymphoma were analysing using a non-linear mixed-effects model technique. 47 SNPs in 16 MTX-related genes were genotyped and screened as covariates. A PPK model was established...

Additional file 1: Table S1. Scoring system for histological changes in the colon. Table S2. The sequences of primers used in this study. Figure S1. ERβ deficiency did not influence the sensorimotor function, memory function, or social interactions in mice following induced experimental colitis. (A) Nest score in the nest building test was performed among the four groups to detect the sensorimotor functions. (B) Spatial memory was assessed by percentage of spontaneous alterations in the...

Additional file 1. PRISMA-P 2015 checklist.

Additional file 2. Databases and search strategies.

Additional file 1.

Additional file 3: Supplemental Table 3. Cognitive performance of participants using different reading materials.

Organic aerosol emitted from cooking is a major concern for indoor air quality. During the House Observations of Microbial and Environmental Chemistry (HOMEChem) campaign, we simulated cooking, cleaning and occupancy activities in a realistic residential setting and measured resulting gas- and particle-phase emissions using a High-Resolution Time-of-Flight Chemical Ionization Mass Spectrometer with a Filter Inlet for Gases and Aerosols (FIGAERO-HR-ToF-CIMS). We identified ∼480 molecular formulas for compounds emitted on cooking-centered days and attributed them to...

Additional file 1: TableS1 Comparison of GMV volume between two groups.

Human activities interfere with natural Phosphorus (P) cycles by introducing increased levels of P emissions to air, land, and water. A supply-side analysis of P supply chains and associated P emissions can provide insights into underlying economic activities and transitions responsible for human-induced P emissions. Taking the mainland China as the case, this study constructs time-series physical input-output tables to describe P supply chains during 1949–2012. Subsequently, it identifies critical products and influencing factors of...

Additional file 1. Table S1. The primers used for real-time PCR.