Supplemental material, sj-docx-7-taj-10.1177_20406223221143245 for Chest X-ray features facilitate screening for pulmonary hypertension caused by fibrosing mediastinitis by Mingfang Zhou, Bo Li, Yaling Chen, Aqian Wang, Yining Zhu, Yu Li, Hongling Su, Jingchun Fan, Yan Zhang and Yunshan Cao in Therapeutic Advances in Chronic Disease

Two new aspidosperma-type monoterpenoid indole alkaloids, 16-O-methylvoafinine (1) and 14,15-diepi-voafinidine (2) were isolated from the aerial parts of Ervatamia officinalis. Their structures were established by comprehensive spectroscopic analysis including 1D and 2D NMR, HR-ESI-MS, and electronic circular dichroism calculation. The isolated compounds were evaluated for cytotoxic activities against HepG2, MCF-7, and A549 cell lines by CCK-8 assay.

A new series of 5-trifluoromethylpyrimidine derivatives were designed and synthesised as EGFR inhibitors. Three tumour cells A549, MCF-7, PC-3 and EGFR kinase were employed to evaluate their biological activities. The results were shown that most of the target compounds existed excellent antitumor activities. In particular, the IC50 values of compound 9u (E)-3-((2-((4-(3-(3-fluorophenyl)acrylamido)phenyl)amino)-5-(trifluoromethyl)pyrimidin-4-yl)amino)-N-methylthiophene-2-carboxamide against A549, MCF-7, PC-3 cells and EGFR kinase reached to 0.35 μM, 3.24 μM, 5.12 μM, and 0.091 μM, respectively. Additionally, further researches...

A series of pleuromutilin derivatives containing alkylamine and nitrogen heterocycle groups were designed and synthesised under mild conditions. The in vitro antibacterial activity of these semisynthetic derivatives against four strains of Staphylococcus aureus (MRSA ATCC 43300, S.aureus ATCC 29213, S.aureus AD3, and S.aureus 144) were evaluated by the broth dilution method. Compound 13 was found to have excellent antibacterial activity against MRSA (MIC = 0.0625 μg/mL). Furthermore, compound 13 was further studied by the time-killing...

The integration of nanofiltration with catalysis enables high-efficient removal of micropollutants due to its remarkable high selectivity and superior degradation efficacy. Meanwhile, the catalysis-assisted nanofiltration can largely alleviate the issues of inferior low-molecular-weight micropollutants selectivity, membrane fouling and tradeoff limitation. Two different technical routes, catalytic coupled nanofiltration system and catalytic nanofiltration membrane, have been developed based on nanofiltration module and material design for micropollutants separation enhancement. In this review, we first summarize the underlying governing...

Chemotherapy is the primary treatment for advanced non-small-cell lung cancer (NSCLC). However, related dose-dependent toxicity limits its clinical use. Therefore, it is necessary to explore new strategies for improving the clinical outcomes while reducing the side effects of chemotherapy in the treatment of NSCLC. In this study, we designed and synthesized epidermal growth factor (EGF)-modified doxorubicin nanoparticles (EGF@DOX-NPs) that selectively targets the epidermal growth factor receptor (EGFR) overexpressed in lung tumor cells. When administered in...

Purpose: Oncology studies employing digital dissection methodologies have provided some insight on the biological features of tumor microenvironment of Triple-negative breast cancer (TNBC), but molecular diagnostics rarely have therapeutic impact. We aimed to identify a novel prognostic biomarker to investigate immune characteristics of TNBC using transcriptomic features. Patients and Methods: We extracted whole transcriptome from breast cancer tissue of 30 TNBC patients and then used bioinformatics approaches to characterize the different immune cell contents in...

Diseases caused by infections, such as helminth infections or viral infections, pose a major human health problem worldwide. In particular, one key reason why helminths cause long-term health and economic burdens is that there is currently no effective targeted vaccine for human use. B cell responses – resulting in the production of antibody – underpin most successful vaccine designs. This thesis uncovered MLL1 as a novel regulator of B cell responses to different pathogens, and...

Accurate traffic flow prediction on the urban road network is an indispensable function of Intelligent Transportation Systems (ITS), which is of great significance for urban traffic planning. However, the current traffic flow prediction methods still face many challenges, such as missing values and dynamic spatial relationships in traffic flow. In this study, a dynamic temporal graph neural network considering missing values (D-TGNM) is proposed for traffic flow prediction. First, inspired by the Bidirectional Encoder Representations...

Additional file 1.

Supplemental Document

The aim of the study was to determine molecular genetic and clinical characterization of acute myeloid leukemia (AML) with trisomy 8 as the sole chromosome abnormality, a recurrent but rare chromosomal abnormality in AML. Interphase fluorescence in situ hybridization, reverse transcriptase-quantitative polymerase chain reaction for gene rearrangement and next-generation sequencing (NGS) were performed on sole trisomy 8 AML patients. A total of 35 AML patients with trisomy 8 as the sole chromosome abnormality were screened....

The aim of the study was to determine molecular genetic and clinical characterization of acute myeloid leukemia (AML) with trisomy 8 as the sole chromosome abnormality, a recurrent but rare chromosomal abnormality in AML. Interphase fluorescence in situ hybridization, reverse transcriptase-quantitative polymerase chain reaction for gene rearrangement and next-generation sequencing (NGS) were performed on sole trisomy 8 AML patients. A total of 35 AML patients with trisomy 8 as the sole chromosome abnormality were screened....

Identifying optimal treatments for patients living with rare diseases is challenging due to the small numbers of individuals affected. One design used to address this challenge is known as the small n, sequential, multiple assignment, randomized trial (snSMART). To investigate the efficacy of an active drug measured by a continuous outcome tested at a low and high dose compared to placebo, we propose a new two-stage snSMART design. In stage 1, patients are randomized to...

Additional file 2. Supplementary methods and materials.

Heterotopic pregnancy (HP) is a rare but potentially life-threatening event with a high risk of maternal death, which also jeopardise the coexisting intrauterine pregnancy (IUP), thus an early accurate diagnosis and prompt treatment can decrease adverse complications. We aimed to explore the early predictors for pregnancy outcomes of HP. We reviewed patients with HP following assisted reproductive technology in our institution between January 2013 and December 2020. The relationships between pregnancy outcomes and clinical features...

Additional file 1.

Traditional robust parameter design methods mainly focus on the optimization of quantitative quality characteristics. However, computer experiments involving qualitative and quantitative mixed input and output occur frequently in the manufacturing industry, which prompted the authors to develop an effective meta-modeling and optimization technique for such experiments. This article combines the latent variable Gaussian process (LVGP) model and fuzzy set theory to create a mixed multi-response LVGP (MMR-LVGP) model involving qualitative and quantitative mixed input and...

The genus Sinocyclocheilus, comprises a large radiation of freshwater cavefishes, are well known for their presence of regressive features (e.g. variable eye reduction). Fewer constructive features are known, such as the expansion of the lateral line system (LLS), which is involved in detecting water movements. The precise relationship between LLS expansion and cave adaptation is not well understood. Here, we examine morphology and LLS-mediated behaviour in Sinocyclocheilus species characterized by broad variation in eye size,...

Supplemental Material for Construction of a Carcinoembryonic Antigen Surface-Enhanced Raman Spectroscopy (SERS) Aptamer Sensor Based on the Silver Nanorod Array Chip by Rui Li, Lijun Li, Yan Zhang, Xin Lin, Heyuanxi Guo, Chubing Lin, and Jun Feng in Applied Spectroscopy

Supplemental Material for Predictive value of adenoid-nasopharyngeal ratio in the diagnosis of secretory otitis media by Ping Li, Tao Li, Liang Yu, Aiping Chen, Yisha Wu, Yuzhu Wan and Li Shi in Ear, Nose & Throat Journal

A phytochemical investigation of Menispermum dauricum led to the isolation of five oxoisoaporphine-type alkaloids (1–5) and five aporphine-type alkaloids (6–10), including a novel oxoisoaporphine-type alkaloid: menispeimin A (1). Their structures were elucidated by spectroscopic studies including MS, 1 D and 2 D NMR, and confirmed by comparing with literature data. Among them, alkaloids 4–10 were obtained for the first time from Menispermum genus. Natural products 2, 4 and 6 exhibited significant cytotoxic activity against A549,...

With the aim of shedding further light on the role of environmental factors in amyotrophic lateral sclerosis (ALS) etiology, we hereby conducted a historical narrative review to critically appraise the published reports on ALS geographical clusters using the modern interpretation of the Bradford Hill criteria for causation. Our research hypothesis was that the more criteria were met, the greater was the evidence supporting a causal association. We found that cluster studies that met the greatest...

A high-dimensional neural network interatomic potential was developed and used in molecular dynamics simulations of condensed phase Ni and Ni systems with liquid–solid phase coexistence. The reference data set was generated by sampling the potential energy surface over a broad temperature-pressure domain using ab initio MD simulations to train a unified potential. Excellent agreement was achieved between bulk face-centred cubic nickel thermal expansion simulations and relevant experimental data. The same potential also yields accurate structures...

supplementary materials