3,738 Works

The Crohn Disease-associated ATG16L1T300A polymorphism regulates inflammatory responses by modulating TLR- and NLR-mediated signaling

Ping Gao, Hongtao Liu, Huarong Huang, Yu Sun, Baoqian Jia, Baidong Hou, Xuyu Zhou, Warren Strober & Fuping Zhang
The mechanisms by which the ATG16L1T300A polymorphism affects cell function and causes an increased risk for the development of Crohn disease remain incompletely understood. Here we report that healthy individuals and mice bearing this polymorphism, even as heterozygotes, manifest enhanced TLR, and NLR cytokine and chemokine responses due to increased activation of NFKB. We elucidated the mechanism of the NFKB abnormality and found that in the ATG16L1T300A cell, there is enhanced polyubiquitination of TRAF6 or...

The Crohn Disease-associated ATG16L1T300A polymorphism regulates inflammatory responses by modulating TLR- and NLR-mediated signaling

Ping Gao, Hongtao Liu, Huarong Huang, Yu Sun, Baoqian Jia, Baidong Hou, Xuyu Zhou, Warren Strober & Fuping Zhang
The mechanisms by which the ATG16L1T300A polymorphism affects cell function and causes an increased risk for the development of Crohn disease remain incompletely understood. Here we report that healthy individuals and mice bearing this polymorphism, even as heterozygotes, manifest enhanced TLR, and NLR cytokine and chemokine responses due to increased activation of NFKB. We elucidated the mechanism of the NFKB abnormality and found that in the ATG16L1T300A cell, there is enhanced polyubiquitination of TRAF6 or...

Circular RNA 0000654 facilitates the growth of gastric cancer cells through absorbing microRNA-149-5p to up-regulate inhibin-beta A

Han Liu & Wen Dai
Circular (circ) RNAs are differentially expressed in gastric cancer (GC) and participate in the biological growth of tumor cells. Given that, investigations were performed to unravel the function of circ_0000654 in GC. GC tissue and normal tissue specimens were obtained, in which circ_0000654, microRNA (miR)-149-5p, and inhibin-beta A (INHBA) levels were examined. GC cell line (BGC-823) was transfected to alter circ_0000654 and miR-149-5p expression, thereby observing cell malignancy. Stably-transfected BGC-823 cells were injected into nude...

Circular RNA 0000654 facilitates the growth of gastric cancer cells through absorbing microRNA-149-5p to up-regulate inhibin-beta A

Han Liu & Wen Dai
Circular (circ) RNAs are differentially expressed in gastric cancer (GC) and participate in the biological growth of tumor cells. Given that, investigations were performed to unravel the function of circ_0000654 in GC. GC tissue and normal tissue specimens were obtained, in which circ_0000654, microRNA (miR)-149-5p, and inhibin-beta A (INHBA) levels were examined. GC cell line (BGC-823) was transfected to alter circ_0000654 and miR-149-5p expression, thereby observing cell malignancy. Stably-transfected BGC-823 cells were injected into nude...

A metal tolerance protein, MTP10, is required for the calcium and magnesium homeostasis in Arabidopsis

Haiman Ge, Qiaolin Shao, Jinlin Chen, Jiahong Chen, Xueqin Li, Yu Tan, Wenzhi Lan, Lei Yang & Yuan Wang
Nutrient antagonism typically refers to the fact that too high a concentration of one nutrient inhibits the absorption of another nutrient. In plants, Ca2+ (Calcium) and Mg2+ (Magnesium) are the two most abundant divalent cations, which are known to have antagonistic interactions. Hence, maintaining their homeostasis is crucial for plant growth and development. In this study, we showed that MTP10 (Metal Tolerance Protein 10) is an important regulator for maintaining homeostasis of Mg and Ca...

A prognostic nomogram for intrahepatic progression-free survival in patients with colorectal liver metastases after ultrasound-guided percutaneous microwave ablation

Si Qin, Huabin Hu, Rui Cui, Jing Lin, Yiming Liu, Yimin Wang, Yao Chen & Guangjian Liu
The present study identified predictors of intrahepatic progression-free survival (ihPFS) after ultrasound (US)-guided percutaneous microwave ablation (MWA) for colorectal liver metastases (CRLMs) and developed a nomogram to predict ihPFS. From January 2013 to December 2018, a total of 314 patients (224 men and 90 women; mean age, 57.1 ± 11.8 years; range, 23–86 years) with 645 CRLMs (mean diameter, 16.6 ± 6.5 mm; range, 6.0–45.0 mm) treated with US-guided percutaneous MWA were included and analyzed....

Defective autophagy contributes to endometrial epithelial-mesenchymal transition in intrauterine adhesions

Zhenhua Zhou, Huiyan Wang, Xiwen Zhang, Minmin Song, Simin Yao, Peipei Jiang, Dan Liu, Zhiyin Wang, Haining Lv, Ruotian Li, Ying Hong, Jianwu Dai, Yali Hu & Guangfeng Zhao
Intrauterine adhesions (IUA), characterized by endometrial fibrosis, is a common cause of uterine infertility. We previously demonstrated that partial epithelial-mesenchymal transition (EMT) and the loss of epithelial homeostasis play a vital role in the development of endometrial fibrosis. As a pro-survival strategy in maintaining cell and tissue homeostasis, macroautophagy/autophagy, conversely, may participate in this process. However, the role of autophagy in endometrial fibrosis remains unknown. Here, we demonstrated that autophagy is defective in endometria of...

Primary graft failure following allogeneic hematopoietic stem cell transplantation: risk factors, treatment and outcomes

Juan Chen, Aiming Pang, Yuanqi Zhao, Li Liu, Runzhi Ma, Jialin Wei, Xin Chen, Yi He, Donglin Yang, Rongli Zhang, Weihua Zhai, Qiaoling Ma, Erlie Jiang, Mingzhe Han, Jiaxi Zhou & Sizhou Feng
Graft failure (GF) is an intractable complication of transplantation, which can severely affect the efficacy of the graft; however, the characteristics, incidence, and risk factors of primary GF have not been well described. This study aimed to analyze the risk factors and outcomes of primary GF to swiftly identify high-risk patients for GF. We performed a case-control study with a case-control ratio of 1:4 with 869 patients who underwent allogeneic hematopoietic stem cell transplantation (allo-HSCT)...

AFAP1 antisense RNA 1 promotes retinoblastoma progression by sponging microRNA miR-545-3p that targets G protein subunit beta 1

Wenting Tang, Li Zhang, Jing Li & Yu Guan
The oncogenic role of actin filament-associated protein 1 antisense RNA 1 (AFAP1-AS1) has been reported in retinoblastoma (RB). However, the underlying regulatory mechanisms remain poorly understood. In this study, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and Western blotting were performed to analyze the expression of AFAP1-AS1, microRNA miR-545-3p, or G protein subunit beta 1 (GNB1). Cell Counting Kit-8 (CCK-8) and Transwell migration assays were used to detect cell proliferation and migration. In addition, caspase-3 activity...

Classification and expression analysis of cucumber (Cucumis sativus L.) callose synthase (CalS) family genes and localization of CsCalS4, a protein involved in pollen development

Hongyun Wang, Shoujun Cao, Tao Li, Lili Zhang, Jiangang Yao, Xiubo Xia & Ruiqing Zhang
Callose plays an important role in plant development and response to multiple biotic and abiotic stresses. Callose synthase (CalS; EC 2.4.1.34), the main enzyme in callose synthesis, has been studied in a number of crops but not in cucumber (Cucumis sativus L.). In this study, 10 genes encoding putative callose synthase were identified and classified into four clades. Expression levels of the 10 CsCalS genes, which varied considerably, were detected in most analyzed organs. Transcription...

Discovery of novel paeonol-based derivatives against skin inflammation in vitro and in vivo

Jing Wu, Ren De Zhu, Guo Min Cao, Jun Cheng Du, Xin Liu, Liang Zhuo Diao, Zhao Yan Zhang, Yang Sheng Hu, Xin Hua Liu & Jing Bo Shi
T-LAK-cell-originated protein kinase (TOPK), a novel member of the mitogen-activated protein kinase family, is considered an effective therapeutic target for skin inflammation. In this study, a series (A − D) of paeonol derivatives was designed and synthesised using a fragment growing approach, and their anti-inflammatory activities against lipopolysaccharide (LPS)-induced nitric oxide production in RAW264.7 cells were tested. Among them, compound B12 yielded the best results (IC50 = 2.14 μM) with low toxicity (IC50 > 50...

Angiogenesis and anti-leukaemia activity of novel indole derivatives as potent colchicine binding site inhibitors

Yongfang Yao, Tao Huang, Yuyang Wang, Longfei Wang, Siqi Feng, Weyland Cheng, Longhua Yang & Yongtao Duan
The screened compound DYT-1 from our in-house library was taken as a lead (inhibiting tubulin polymerisation: IC50=25.6 µM, anti-angiogenesis in Zebrafish: IC50=38.4 µM, anti-proliferation against K562 and Jurkat: IC50=6.2 and 7.9 µM, respectively). Further investigation of medicinal chemistry conditions yielded compound 29e (inhibiting tubulin polymerisation: IC50=4.8 µM and anti-angiogenesis in Zebrafish: IC50=3.6 µM) based on tubulin and zebrafish assays, which displayed noteworthily nanomolar potency against a variety of leukaemia cell lines (IC50= 0.09–1.22 µM), especially...

A transcriptomic analysis of neuropathic pain in the anterior cingulate cortex after nerve injury

Yu Zhang, Shiwei Jiang, Fei Liao, Zhifeng Huang, Xin Yang, Yu Zou, Xin He, Qulian Guo & Changsheng Huang
The anterior cingulate cortex (ACC) is a core brain region processing pain emotion. In this study, we performed RNA sequencing analysis to reveal transcriptomic profiles of the ACC in a rat chronic constriction injury (CCI) model. A total of 1628 differentially expressed genes (DEGs) were identified by comparing sham-operated rats with rats of 12 hours, 1, 3, 7, and 14 days after surgery, respectively. Although these inflammatory-related DEGs were generally increased after CCI, different kinetics...

Kavalactones isolated from Alpinia zerumbet (Pers.) Burtt. et Smith with protective effects against human umbilical vein endothelial cell damage induced by high glucose

Hualin You, Min He, Di Pan, Guanqin Fang, Yan Chen, Xu Zhang, Xiangchun Shen & Nenling Zhang
A new kavalactone, 4'-hydroxyl dihydro-5, 6-dehydrokavain (1) was isolated from the petroleum ether partition of leaves of Alpinia zerumbet (Pers.) Burtt. et Smith, together with four known kavalactone dimers, rel-1,trans-3-bis-(4-methoxy-2-oxopyran-6-yl)-cis-2,trans-4-diphenyl cyclobutene (2), aniba dimer A (3), aniba dimer C (4), 6,6'-(3,4-diphenylcyclobutane-1,2-diyl)bis(4-methoxy-2H-pyran-2-one (5). The structure of compound 1 was characterized by its MS, 1D-NMR, and 2D-NMR data, and the structures of the known compounds were determined by comparison of their spectroscopic data with those reported by...

Hepatoprotective mechanism of Silybum marianum on nonalcoholic fatty liver disease based on network pharmacology and experimental verification

Guoyan Jiang, Chunhong Sun, Xiaodong Wang, Jie Mei, Chen Li, Honghong Zhan, Yixuan Liao, Yongjun Zhu & Jingxin Mao
The study aimed to identify the key active components in Silybum marianum (S. marianum) and determine how they protect against nonalcoholic fatty liver disease (NAFLD). TCMSP, DisGeNET, UniProt databases, and Venny 2.1 software were used to identify 11 primary active components, 92 candidate gene targets, and 30 core hepatoprotective gene targets in this investigation, respectively. The PPI network was built using a string database and Cytoscape 3.7.2. The KEGG pathway and GO biological process enrichment,...

Nitrogen addition frequency and propagule pressure influence Solidago canadensis invasion into native plant community

Xuan-Shao Liu, Jing-Fang Cai, Lin Liu, Kai Sun, Fan Jiang, Yi-Luan Shen, Si-Ha A & Hong-Li Li
Propagule pressure (i.e., the number of propagules) has long been recognized to play an essential role in plant invasion. But it is not clear whether propagule pressure influences the invasion of exotic plants into native plant communities when different frequencies of nitrogen are added. We established an experiment with three plant communities that included native plant communities alone (four grasses, two legumes and two forbs) or native plant communities with one or five invasive plants,...

TET2 regulates osteoclastogenesis by modulating autophagy in OVX-induced bone loss

Chen Yang, Huaqiang Tao, Haifeng Zhang, Yu Xia, Jiaxiang Bai, Gaoran Ge, Wenming Li, Wei Zhang, Long Xiao, Yaozeng Xu, Zhirong Wang, Ye Gu, Huilin Yang, Yu Liu & Dechun Geng
Increased bone resorption by osteoclasts after estrogen deficiency is the main cause of postmenopausal osteoporosis. TET2 (tet methylcytosine dioxygenase 2) is a DNA demethylase that regulates cellular function and differentiation potential. Macroautophagy/autophagy maintains cellular homeostasis by recycling unnecessary and damaged organelles. This study revealed that TET2 promoted bone loss in oophorectomized (OVX) mice and that TET2 promoted osteoclast differentiation by regulating autophagy. Tet2 knockdown inhibited autophagy and osteoclast differentiation in vitro. Mechanistically, Tet2 knockdown increased...

Pharmacokinetic study of Tangwang Mingmu granule for the management of diabetic retinopathy based on network pharmacology

Yucheng Wang, Beibei Xue, Xiaoli Wang, Qilong Wang, Erwei Liu & Xiaopeng Chen
Tangwang Mingmu granule (TWMM), a traditional Chinese medicine, has been widely used in the treatment of diabetic retinopathy (DR), the most common microvascular complication in diabetes mellitus. To establish a method to select target compounds from herbs for a pharmacokinetic study using network pharmacology, which could be applied in clinical settings. First, UPLC/Q Exactive Q-Orbitrap and GCMS 2010 were used to determine the non-volatile and volatile ingredients of TWMM. Based on the identified compounds, network...

Pharmacokinetic study of Tangwang Mingmu granule for the management of diabetic retinopathy based on network pharmacology

Yucheng Wang, Beibei Xue, Xiaoli Wang, Qilong Wang, Erwei Liu & Xiaopeng Chen
Tangwang Mingmu granule (TWMM), a traditional Chinese medicine, has been widely used in the treatment of diabetic retinopathy (DR), the most common microvascular complication in diabetes mellitus. To establish a method to select target compounds from herbs for a pharmacokinetic study using network pharmacology, which could be applied in clinical settings. First, UPLC/Q Exactive Q-Orbitrap and GCMS 2010 were used to determine the non-volatile and volatile ingredients of TWMM. Based on the identified compounds, network...

Water-soluble chitosan and phytoremediation efficiency of two Brassica napus L. cultivars in cadmium-contaminated farmland soils

Jian-Lin Bian, Wei Cao, Jun-Mei Guo, Jun-Xing Yang, Xue-Dong Wang, Jie Wang, Jun Huang, Tian-Xiang Xia & Cun-Yan Xia
Pot and field trials were conducted to investigate Cd uptake and phytoremediation efficiency of two Brassica napus cultivars (QY-1 and SYH) with applied water-soluble chitosan (WSC, Pot: 0, 2% and 4%; Field: 0 and 10 g·m−2) grown in Cd-contaminated soils. The results from the pot and field trials generally showed that WSC treatments significantly increased Cd concentrations in shoot and root tissues by 33.77–159.71% (except for SYH/JY) and 7.42–168.71% of two B. napus cultivars compared...

Bidirectional regulation of structural damage on autophagy in the C. elegans epidermis

Rong Fu, Xiaowan Jiang, Yuyan Yang, Chunxia Wang, Yun Zhang, Yi Zhu & Huimin Zhang
A variety of disturbances such as starvation, organelle damage, heat stress, hypoxia and pathogen infection can influence the autophagic process. However, how the macroautophagy/autophagy machinery is regulated intrinsically by structural damage of the cell remains largely unknown. In this work, we utilized the C. elegans epidermis as the model to address this question. Our results showed that structural damage by mechanical wounding exerted proximal inhibitory effect and distant promotional effect on autophagy within the same...

Upstream open reading frames mediate autophagy-related protein translation

Ying Yang, Damián Gatica, Xu Liu, Runliu Wu, Rui Kang, Daolin Tang & Daniel J. Klionsky
Macroautophagy/autophagy, a highly conserved catabolic pathway that maintains proper cellular homeostasis is stringently regulated by numerous autophagy-related (Atg) proteins. Many studies have investigated autophagy regulation at the transcriptional level; however, relatively little is known about translational control. Here, we report the upstream open reading frame (uORF)-mediated translational control of multiple Atg proteins in Saccharomyces cerevisiae and in human cells. The translation of several essential autophagy regulators in yeast, including Atg13, is suppressed by canonical uORFs...

The response surface optimization of β-mannanase produced by Weissella cibaria F1 and its potential in juice clarification

Hairui Ji, Huiying Cao, Li Zhao, Ruiying Na, Wenxiang Ping, Jingping Ge & Dan Zhao
A β-mannanase-producing lactic acid bacteria (LAB) was identified as Weissella cibaria F1 according to physiological and biochemical properties, morphological observations, partial sequence of 16S rRNA gene and API 50 CHL test. In order to improve the yield of β-mannanase, the response surface methodology (RSM) was originally used to optimize the fermentation conditions. The optimization results showed that when the konjac powder, glucose, and initial pH were 9.46 g/L, 14.47 g/L and 5.67, respectively, the β-mannanase...

Sodium houttuyfonate enhances the mono-therapy of fluconazole on oropharyngeal candidiasis (OPC) through HIF-1α/IL-17 axis by inhibiting cAMP mediated filamentation in Candida albicans-Candida glabrata dual biofilms

Mengli Chen, Ting Cheng, Chen Xu, Min Pan, Jiadi Wu, Tianming Wang, Daqiang Wu, Guiming Yan, Changzhong Wang & Jing Shao
Candida albicans and Candida glabrata are two common opportunistic fungi that can be co-isolated in oropharyngeal candidiasis (OPC). Hypha is a hallmark of the biofilm formation of C. albicans, indispensable for the attachment of C. glabrata, which is seldom in mycelial morphology. Increasing evidence reveals a hypoxic microenvironment in interior fungal biofilms, reminding of a fact that inflammation is usually accompanied by oxygen deprivation. As a result, it is assumed that the disaggregation of hypha-mediated...

Sodium houttuyfonate enhances the mono-therapy of fluconazole on oropharyngeal candidiasis (OPC) through HIF-1α/IL-17 axis by inhibiting cAMP mediated filamentation in Candida albicans-Candida glabrata dual biofilms

Mengli Chen, Ting Cheng, Chen Xu, Min Pan, Jiadi Wu, Tianming Wang, Daqiang Wu, Guiming Yan, Changzhong Wang & Jing Shao
Candida albicans and Candida glabrata are two common opportunistic fungi that can be co-isolated in oropharyngeal candidiasis (OPC). Hypha is a hallmark of the biofilm formation of C. albicans, indispensable for the attachment of C. glabrata, which is seldom in mycelial morphology. Increasing evidence reveals a hypoxic microenvironment in interior fungal biofilms, reminding of a fact that inflammation is usually accompanied by oxygen deprivation. As a result, it is assumed that the disaggregation of hypha-mediated...

Registration Year

  • 2022
    3,738

Resource Types

  • Text
    3,738

Affiliations

  • Shanghai Jiao Tong University
    3,738
  • Chinese Academy of Medical Sciences & Peking Union Medical College
    2,400
  • Zhejiang University
    2,037
  • Sichuan University
    1,906
  • Fudan University
    1,906
  • Sun Yat-sen University
    1,802
  • Capital Medical University
    1,777
  • Huazhong University of Science and Technology
    1,699
  • Chinese Academy of Sciences
    1,549
  • Nanjing Medical University
    1,537