Additional file 1. Detailed methods or results covered in this article.

Additional file 1.

Additional file 2: Figure S2. Selection of the optimal number of clusters.

Additional file 3: Figure S2. Genetic barriers for the snail populations based on Monmonier’s algorithm.

Additional file 3: Figure S2. Genetic barriers for the snail populations based on Monmonier’s algorithm.

Additional file 5: Figure S4. A Kruskal-Wallis comparison analysis of median distances among different groups.

Additional file 8: Table S5. Clinical information of TCGA dataset.

Additional file 8: Table S5. Clinical information of TCGA dataset.

Additional file 2: Figure S2. A Venn diagram shows the overlap of 22 optimal hub genes between GEO differential genes and WGCNA grey gene set.

Supplemental material, sj-docx-1-aim-10.1177_09645284221117847 for Electroacupuncture alleviates cognitive dysfunction and neuronal pyroptosis in septic mice by Yan Li, Zhaoying Li, Fujuan He, Chenguang Qin, Rui Fan, Fangxiang Zhang and Bin Wang in Acupuncture in Medicine

Melittin is a natural polypeptide present in bee venom, with significant anti-tumor activity. Melittin has been reported to induce cell death in lung carcinoma cell line A549 cells, suggesting an excellent potential for treating lung cancer. However, the core mechanism underlying melittin-induced cell death in A549 cells remains unclear. This work reports that melittin induces reactive oxygen species (ROS) burst, upregulates intracellular Fe2+ levels, disrupts the glutathione-glutathione peroxidase 4 antioxidant system, and increases lipid peroxide...

Illustration of beam drift, and configuration of algorithm

supplementary statement

Additional file 1: Fig. S1. Repeated verification of the interaction between HCR and astrin. (A) Astrin Co-IP HCR and CEP72 and HCR Co-IP astrin and CEP72. HeLa cell lysates were immunoprecipitated with astrin, HCR, or control rabbit IgG antibodies and analyzed by western blotting with anti-astrin, anti-HCR antibodies. Anti-GM130 and anti-beta actin antibodies were used as negative control. The blotting of astrin, HCR and GM130 antibodies were incubated on the same membrane by repeatedly washing...

Additional file 3: Fig. S3. Apoptosis or cycle changes in astrin-KO cells and identification of astrin-KO cell line. (A) Parental, astrin-KO and HCR-KO HeLa cells were analyzed with astrin, HCR, cyclin B1 and Cleaved PARP antibodies. HeLa cells treated with DMSO, Paclitaxel, Bafilomycin and MG132 were analyzed with Cleaved PARP and HCR antibodies. (B) Parental and astrin-KO HeLa cells were immunoblotted with an astrin antibody.

Additional file 5: Fig. S8. Co-localization of HCR with centrin1 and PCM1. HeLa cells transfected with HCR-GFP were co-stain with centrin1 (red) and gamma-tubulin (cyan) and DAPI (blue) or PCM1 (red) and gamma-tubulin (cyan) and DAPI (blue); scale bars, 10 μm. Fig. S9. HCR interacts with CEP131. HeLa cell lysates were immunoprecipitated with antibodies specific for HCR, astrin, or negative control rabbit IgG. The precipitates were analyzed by immunoblotting with antibodies against HCR and CEP131....

Additional file 8. Verification reports of HCR-KO cell line.

Additional file 9. Verification reports of astrin-KO cell line.

Supplemental Document

supplemental document of the manuscript

Supplementary Material 1

Supplementary Material 1

Additional file 5: Fig. S2. Expression levels of lncRNAs and PCgenes in sunflower endosperm.

Additional file 1: Table S1. ICD codes used to define medical diagnoses and surgical procedures.

Additional file 1: Figure S1. Pairwise sequence identify matrix for plant PR10/Bet v1-like protein sequences. Red and green boxes indicate high and low pairwise identity. Figure S2. Architecture of conserved regions in plant PR10/Bet v1-like proteins.