Streptococcus suis is a highly invasive pathogen that can cause sepsis and meningitis in pigs and humans. However, we have limited understanding of the mechanisms S. suis uses to evade innate immunity. To investigate the involvement of the two-component signal transduction system of S. suis in host immune defense, we examined the expression of 15 response regulators of S. suis following stimulation with polymorphonuclear leukocytes (PMNs). We found that several response regulators were significantly up-regulated...

Ropivacaine is a commonly used local anaesthetic, but its side effects remain largely unknown. In the present study, we investigated the side effects of ropivacaine in human neuronal SH-5Y5Y cells. We show that 0.5% and 1% ropivacaine could cause fission-like mitochondrial morphological changes. Ropivacaine exclusively induces mitochondrial fission protein DRP1, generation of ROS and causes mitochondrial dysfunction including decreasing mitochondrial membrane potential, the activity of cytochrome C oxidase and ATP production. The side effects of...

In order to devise more effective penetration enhancers, 4-O-acylterpineol derivatives which were expected to be hydrolyzed into nontoxic metabolites by esterase in the living epidermis, were synthesized from 4-terpineol (4-TER) enantiomers and straight chain fatty acids. Their promoting activities on the SR-flurbiprofen and its enantiomers were tested across full-thickness rabbit skin, as well as to correlate under in vitro and in vivo conditions. The permeation studies indicated that both d-4-O-acylterpineol and l-4-O-acylterpineol had significant enhancing...

Scabies remains a significant public health concern globally, affecting people of all ages, races, and socioeconomic groups. The epidemic characteristics of scabies are yet unravelled, especially in high-income countries and elderly population. In this study, we sought to investigate incidence and prevalence and their trends of scabies worldwide from 1990 to 2017 via the Global Burden of Disease (GBD) Study 2017. The prevalence and incidence of scabies tend to a moderate increase after age 70....

The adsorption of phages to hosts is the first step of phage infection. Studies have shown that tailed phages use tail fibres or spikes to recognize bacterial receptors and mediate adsorption. However, whether other phage tail components can also recognize host receptors is unknown. To identify potential receptors, we screened a transposon mutagenesis library of the marine pathogen Vibrio parahaemolyticus and discovered that a vp0980 mutant (vp0980 encodes a predicted transmembrane protein) could not be...

Scabies remains a significant public health concern globally, affecting people of all ages, races, and socioeconomic groups. The epidemic characteristics of scabies are yet unraveled, especially in high-income countries and elderly population. In this study, we sought to investigate incidence and prevalence and their trends of scabies worldwide from 1990 to 2017 via the Global Burden of Disease (GBD) Study 2017. The prevalence and incidence of age-standardized rate (ASR) of scabies tend to a moderate...

One of the major goals in HIV-1 vaccine development is to achieve properly folded and stabilized envelope glycoprotein (Env) trimers that mimic the native Env on the mature virion. Here, we design and characterize uncleaved prefusion-optimized (UFO) trimers for 12 Envs currently circulating in China. Biochemical and biophysical characterization of these UFO trimers identified two subtype B/Bʹ Envs, CNE6 and MG13, which exhibited the highest trimer content and stability at a level comparable to the...

One of the major goals in HIV-1 vaccine development is to achieve properly folded and stabilized envelope glycoprotein (Env) trimers that mimic the native Env on the mature virion. Here, we design and characterize uncleaved prefusion-optimized (UFO) trimers for 12 Envs currently circulating in China. Biochemical and biophysical characterization of these UFO trimers identified two subtype B/Bʹ Envs, CNE6 and MG13, which exhibited the highest trimer content and stability at a level comparable to the...

A simple, eco-friendly and convenient procedure has been developed for the synthesis of diphenyl diselinides from readily available aryl boronic acids by reaction with SeO2 in the presence of iodine under simple conditions. The methodology is reported about 19 examples and applicable to a broad scope of aryl boronic acids containing electron-rich and electron-poor substituents. Apart from being milder and environmentally benign conditions, this synthetic protocol comprises a novel, reliable methodology to give good to...

Tick-borne encephalitis virus (TBEV) accounts for approximately 10,000 annual cases of severe encephalitis in Europe and Asia and causes encephalitis in humans. In this study, we demonstrate TBEV appears to activate the interferon (IFN)-β dependent on RIG-I/MDA5. Both the IFN-β accumulation and the IFN stimulated genes (ISGs) transcription greatly delay. Further studies reveal that TBEV NS4A could block the phosphorylation and dimerization of STAT1/STAT2 to affect type I and II IFN-mediated STAT signalling. Additional data...

One new butylphthalide ligusticumolide H (1), together with the known butylphthalides senkyunolide I (2), senkyunolide H (3), (3 R,3′S)-3′-hydroxy-3-butylphthalide (4), (3 R)-4-hydroxy-3-butylphthalide (5) and senkyunolide C (6) was isolated from the rhizome of Ligusticum striatum DC. Their structures were determined by extensive spectroscopic analyses including HR-ESI-MS, 1D and 2D NMR, and the absolute configuration of 1 was established by NMR and ECD calculations. Their effect on NO production in IL-1β-stimulated chondrocytes was also evaluated.

Supplemental material, sj-docx-1-tag-10.1177_17562848231165129 for Neutrophil-to-lymphocyte ratio for predicting postoperative recurrence in Crohn’s disease patients with isolated anastomotic lesions by Rirong Chen, Chao Li, Kang Chao, Yizhe Tie, Jieqi Zheng, Huili Guo, Zhirong Zeng, Li Li, Minhu Chen and Shenghong Zhang in Therapeutic Advances in Gastroenterology

Supplemental material, sj-docx-1-ltr-10.1177_13621688231164758 for Improving EFL students’ stance-taking in academic writing with SFL-based instruction: A qualitative inquiry by Lu Zhang and Lawrence Jun Zhang in Language Teaching Research

Diabetic kidney disease (DKD) is a severe complication of type 2 diabetes mellitus (T2DM). However, the pathogenesis of DKD remains unclear, and effective treatment strategies are still lacking. Ginsenoside Rg1 (Rg1) has been reported to improve DKD, but the mechanism is unclear. NADPH oxidase 4 (NOX4) is an essential reactive oxygen species (ROS) source in the kidney. The mitogen-activated protein kinase (MAPK) signaling may exacerbate renal fibrosis. Therefore, we hypothesized that Rg1 might alleviate renal...

figures and table

figures and table

Supplemental material, sj-docx-1-ine-10.1177_15910199231164840 for Association between basilar artery stenosis features, vertebral artery stenosis and perforator stroke after stenting by Rui Huang, Bo Yang, Feng Gao, Dapeng Mo, Ming Yang, Zhikai Hou, Yifan Liu, Rongrong Cui, Kaijiang Kang, Weibin Gu, Jianfeng He, Xin Lou, Zhongrong Miao and Ning Ma in Interventional Neuroradiology

Supplemental material, sj-docx-1-cpj-10.1177_00099228231160832 for A Novel IDUA Mutation Causing Ocular Disease in 2 Siblings by Jiali Xia, Min Liu, Xingli Luo, Hong Yang and Ling Yu in Clinical Pediatrics

Localization

Localization

Supplemental material, sj-pdf-1-prf-10.1177_02676591221090588 for A five-parameter score for predicting saphenous vein graft degenerative and/or occlusive disease in recurring ischemic symptoms after one year post coronary artery bypass grafting by Xiao-Wei Li, Zhuang Cui, Jian-Yong Xiao, Ming-Dong Gao, Mei Zhang, Wen-Juan Zhang, Feng-Shi Tian, Yu Song, Ying-Wu Liu, Zhu-Hua Yao, Jun Ma, Yin Liu and Jing Gao in Perfusion

Supplemental Material for Development of a quantitative method to evaluate the printability parameters of water-based ink based on visible and near infrared spectroscopy by Yongli Bai, Xinguo Huang, Nan Peng, Shanshan Zhang, Yunfei Zhong in Journal of Near Infrared Spectroscopy

Supplemental Material for Development of a quantitative method to evaluate the printability parameters of water-based ink based on visible and near infrared spectroscopy by Yongli Bai, Xinguo Huang, Nan Peng, Shanshan Zhang, Yunfei Zhong in Journal of Near Infrared Spectroscopy

Ferroptosis is a newly characterized form of programmed cell death, which is driven by the lethal accumulation of lipid peroxides catalyzed by the intracellular bioactive iron. Targeted induction of ferroptotic cell death holds great promise for therapeutic design against other therapy-resistant cancers. To date, multiple post-translational modifications have been elucidated to impinge on the ferroptotic sensitivity. Here we report that the Ser/Thr protein kinase ATM, the major sensor of DNA double-strand break damage, is indispensable...

In preclinical studies, a new antituberculosis drug regimen markedly reduced the time required to achieve relapse-free cure. This study aimed to preliminarily evaluate the efficacy and safety of this four-month regimen, consisting of clofazimine, prothionamide, pyrazinamide and ethambutol, with a standard six-month regimen in patients with drug-susceptible tuberculosis. An open-label pilot randomized clinical trial was conducted among the patients with newly diagnosed bacteriologically-confirmed pulmonary tuberculosis. The primary efficacy end-point was sputum culture negative conversion. Totally,...