410 Works

Sex Difference in Lipid Levels in First-Diagnosed Drug-Naïve Depression Patients: A Case-Control and 12-Weeks Follow-up Study

Rui Yang, Lu Wang, Song Cao, Ming Chen, Chu-Jun Wu, Floyd Silva, Man-Jun Shen, Jin-Dong Chen, Mi-Mi Tang & Bi-Lian Liu
Patients with depression have a high prevalence of developing dyslipidemia. In this study, we aim to investigate the difference of serum lipids, including total cholesterol (TCH), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), and triglycerides (TG), between the depressed patients and healthy controls. Sex differences in lipids and their psychological correlations were also included. The study included 56 healthy controls (males/females =26/30) and 110 first-diagnosed drug-naïve outpatients (males/females =35/75). A total of 42 patients...

Circular RNA hsa_circ_0075542 acts as a sponge for microRNA-1197 to suppress malignant characteristics and promote apoptosis in prostate cancer cells

Yuefu Han, Xingqiao Wen, Xiaojuan Li, Dong Chen, Lian Peng, Bin Lai & Hongcai Huang
Numerous differentially expressed circular RNAs (circRNAs) have been identified; however, their roles have not been fully elucidated. Since dysregulated circRNAs may have clinical applications, it is vital to study their expression characteristics, function, and mechanism in prostate cancer cells. The role, regulatory mechanism, and expression of hsa_circ_0075542 were analyzed using quantitative reverse transcription polymerase chain reaction. The results indicated that the expression of hsa_circ_0075542 was downregulated in prostate tumor tissues. The functions of prostate cancer...

Three new tyrosol derivatives from Huangjing wine

Yan-Bin Ren, Xing-Wen Wang, Jia-Xuan Bai, Chang Liu, Si-Lin Yu, Yue Zhou, Cong-Cong Lin, Da-Hong Yao, Jian Huang & Jin-Hui Wang
Phytochemical investigation on the concentrate of Huangjing wine, resulted in the isolation of three new tyrosol derivatives 4′′′-hydroxyphenethyl 2-(R)-hydroxy-3-phenylpropionate (1), 4′′′-hydroxyphenethyl(4′-hydroxy-3′-methoxyphenyl)propionate (2) and 4′′-hydroxyphenethyl ethyl succinate (3), together with 5 known compounds, ferulic acid (4), L-phenyllactic acid (5), hydroxytyrosol (6), dihydroferulic acid (7), cyclo(L-Pro-D-Tyr) (8). Their structures were elucidated using spectroscopic analysis and by comparison with the literature data. All compounds displayed antioxidant effect in the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical. Among them, the new compound 2...

Taberdines L and M, two new alkaloids from Tabernaemontana divaricata

Lingling Han, Kepu Huang, Chen Chen, Wentao Zhu, Yehan Ma, Xiaojiang Hao, Hongping He & Yu Zhang
Two new alkaloids, taberdines L (1) and M (2), together with six known alkaloids, were isolated from the twigs and leaves of Tabernaemontana divaricata. Taberdine L (1) represents the first optically active natural member of the allo iboga class of alkaloids with the ethyl side chain in a bridgehead position. Their structures including absolute configuration were elucidated by a combination of MS, NMR, and ECD calculation. The in vitro cytotoxic activities of 1 and 2...

Additional file 2 of Using a WeChat mini-program-based lactation consultant intervention to increase the consumption of mother’s own milk by preterm infants in the neonatal intensive care unit: a study protocol for a cluster randomized controlled trial

Jie Huo, Xinping Wu, Chuanli Gu, Zhangbin Yu, Jun Zhang, Xiaohui Chen, Jingai Zhu, Feng Liu, Beibei Liu, Qianqian Li & Shuping Han
Additional file 2.

Study on longitudinal dynamics of heavy haul trains running on long and steep downhills

Wentao Liu, Shuai Su, Tao Tang & Yuan Cao
This paper investigates the longitudinal dynamics performance of the combined heavy haul trains, especially for the cyclic air braking strategy on the long and steep downhill segments. Firstly, the mathematical models for calculating the longitudinal forces in the longitudinal train dynamics simulations are formulated. The brake delay time between neighbouring cars and the pressure variation of the brake cylinders in the braking and releasing processes are considered in the air braking force model. Besides, the...

Effects of percutaneous balloon mitral valvuloplasty in patients with mitral stenosis and atrial fibrillation: a systematic review and meta-analysis

Bojiang Liu, Yunyun Wang, Yingwu Liu, Peng Wu & Tong Li
The study aimed to systematically review the existing literature and assess the effects of percutaneous balloon mitral valvuloplasty (PBMV) in patients with mitral stenosis and atrial fibrillation (AF) as opposed to sinus rhythm (SR). Eligible studies were identified from six electronic databases before June 2021. The primary outcome was mitral valve area (MVA), and secondary outcomes were hemodynamic measurements, in-hospital complications, and long-term outcomes. Relative risks (RRs) or weighted mean differences (WMDs) with 95% confidence...

Comparative analysis of circular RNA enrichment methods

Huajuan Shi, Ying Zhou, Erteng Jia, Zhiyu Liu, Min Pan, Yunfei Bai, Xiangwei Zhao & Qinyu Ge
The circRNAs sequencing results vary due to the different enrichment methods and their performance is needed to systematic comparison. This study investigated the effects of different circRNA enrichment methods on sequencing results, including abundance and species of circRNAs, as well as the sensitivity and precision. This experiment was carried out by following four common circRNA enrichment methods: including ribosomal RNA depletion (rRNA–), polyadenylation and poly (A+) RNA depletion followed by RNase R treatment (polyA+RNase R),...

Foliar nutrient diagnosis norms for litchi (Litchi chinensis Sonn. cv. Feizixiao) in South China

Donglin Luo, Wei Wang, Cuihua Bai, Luwei Zhu, Huan Li, Changmin Zhou, Quanmin Qiu & Lixian Yao
Foliar nutrient diagnosis is a potent tool for nutrient management in crops. Litchi (Litchi chinensis Sonn.) plantation in China dominates the worldwide litchi cultivation, and Feizixiao is the most widely cultivated species in China. Herein, 538 leaf samples were collected at fruit swelling stage and the terminal shoot maturing stage during 2016–2017, and the fruit yields of all the sampled trees were recorded. The suitability of four nutrient diagnosis methods including critical value approach (CVA),...

Cyclodextrin metal-organic framework as vaccine adjuvants enhances immune responses

Congcong Li, Chaoxi Chen, Yucai Wei, Min Tan, Shuo Zhai, Juebo Zhao, Lu Wang & Tao Dai
It is urgently needed to develop novel adjuvants for improving the safety and efficacy of vaccines. Metal-organic frameworks (MOFs), with high surface area, play an important role in drug delivery. With perfect biocompatibility and green preparation process, the γ-cyclodextrin metal-organic framework (γ-CD-MOF) fabricated with cyclodextrin and potassium suitable for antigen delivery. In this study, we modified γ-CD-MOF with span-85 to fabricate the SP-γ-CD-MOF as animal vaccine adjuvants. The ovalbumin (OVA) as the model antigen was...

Two new dammarane-type ginsenosides from Panax ginseng

Xueni Niu, Xiaoyu Fan, Chongning Lv & Jincai Lu
Two new dammarane-type ginsenosides elucidated as 3-O-[β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl-(1→2)-β-D-glucopyranosyl]-20-O-[α-D-glucopyranosyl-(1→4)-β-D-glucopyranosyl]-20(S)-protopanaxadiol, named Ginsenoside Rb6(2); (Z)-6-O-β-D-glucopyranosyl-dammar-20(22), 24-diene-3β, 6α, 12β-triol, named (20Z)-Ginsenoside Rh4 (4); along with five known ginsenosides(1, 3, 5, 6, 7) were isolated from the radix and rhizome of wild ginseng (Panax ginseng). The structures were determined by chemical and comprehensive spectroscopic analysis, including 1D, 2D NMR, HR-ESI-MS and hydrolysis reaction.

Cloning, homology modeling, heterologous expression and bioinformatic analysis of Ure2pA glutathione S-transferase gene from white rot fungus Trametes gibbosa

Jian Zhang, Yujie Chi, Shuxuan Li, Xinzhi Gu & Yi Ye
Glutathione S-transferases (GSTs) are a class of enzymes with detoxification function. GSTs can be used for the degradation of toxic aromatic compounds, such as dyes. In order to promote their application and cost-effective dye degradation and to understand their structure and function more deeply, we cloned and expressed a gst2 gene from Trametes gibbosa—a white rot fungus. T.gibbosa gst2 has a 672-bp open reading frame (ORF) and encodes 223 amino acids. The recombinant Tg-gst2 in...

Diversification and prevalence of the quinolone resistance crpP genes and the crpP-carrying Tn6786-related integrative and conjugative elements in Pseudomonas aeruginosa

Zhichen Zhu, Huiying Yang, Zhe Yin, Ying Jing, Yuee Zhao, Hongyu Fu, Hong Du & Dongsheng Zhou
The quinolone resistance crpP genes can mediate decreased susceptibility to quinolones. However, diversification and prevalence of crpP genes and crpP-carrying integrative and conjugative elements (ICEs) still need to be elucidated. In this study, genome sequencing was conducted for 200 Chinese Pseudomonas aeruginosa isolates, 16 of which were fully sequenced. All the 37 available CrpP variants were collected for phylogenetic analysis, 10 CrpP enzymes were chosen to conduct cloning and antimicrobial susceptibility test, and 22 crpP-carrying...

Value of quantitative ultrasound and bioelectrical impedance analysis in detecting low bone mineral density in hemodialysis

Ting Xiang, Li Zhou, Ping Fu, Xue-Ping Yan & Xiao-Qing Zeng
Patients on maintenance hemodialysis (MHD) are highly predisposed to low bone mineral density (BMD). This study aims to assess the value of quantitative ultrasound (QUS), bioelectrical impedance analysis (BIA), and their combination in detecting high-risk patients for low BMD in MHD. Patients’ BMD of the total hip, femoral neck, and lumbar spine were measured using dual-energy X-ray absorptiometry (DXA). Bone mineral content (BMC) was assessed using BIA. Calcaneal BMD was measured using QUS. Patients with...

Analysis of the eighth intron polymorphism of NR6A1 gene in sheep and its correlation with lumbar spine number

Taotao Mi, Kaiping Liu, Tao Guo, Lei Li, Yue Wang, Cunyuan Li, Yuying Cui, Jihong Dai, Yunfeng Zhang & Shengwei Hu
For revealing molecular markers related to the traits of multiple lumbar vertebrae in sheep, we analyze the relationship between NR6A1 gene polymorphism and lumbar vertebrae number traits in Xinjiang Kazakh sheep. Lumbar muscle tissues were collected from 6-lumbar spine (L6) Kazak sheep and 7-lumbar spine (L7) Kazak sheep and the intron-8 of NR6A1 gene was amplified by PCR. The SNP locus was detected by the PCR-SSCP method. One-Way ANOVA and an Independent Chi-square Test is...

Study on the working mechanism of fluid loss additive for chlorination titanium blast furnace slag

Ping Zhou, Yuanpeng Wu, Huiting Liu, Ming Li, Weiyuan Xiao, Meng Liu, Jianzhang Hao, Yanming Li & Chengxin Li
The chlorination titanium blast furnace slag belongs to industrial waste, but after grinding, they can be used to prepare cementing fluids to realize the secondary utilization of solid waste resources. As a common additive, fluid loss additives are mostly used in oil well cement systems, and there is still a lot of room for development when applied to chlorination titanium blast furnace slag systems. In this paper, the AMPS/DN-1/DMC/AA tetramer fluid loss additive was synthesized...

Effect of feed efficiency on growth performance, body composition, and fat deposition in growing Hu lambs

Xiaoxue Zhang, Guoze Li, Fadi Li, Deyin Zhang, Lvfeng Yuan, Yuan Zhao, Yukun Zhang, Xiaolong Li, Qizhi Song & Weimin Wang
The present study aimed to investigate the relationship between growth performance, body composition, and fat deposition factors, and feed efficiency in growing lambs. We measured average daily feed intake (ADFI) and body weight (BW) from 653 Hu sheep that were fed a pellet diet. The residual feed intake (RFI) not significantly genetic and phenotypic correlated with the metabolic body weight (MBW) and average daily gain (ADG), but it was significantly genetic and phenotypic correlated with...

Extraction and characterization of a functional protein from Millettia speciosa Champ. leaf

Si-Yuan Luo, Zhi Huang, Xi Chen, Min-Hua Zong & Wen-Yong Lou
Natural plant-derived protein with excellent bioactivities has attracted much attention so a functional protein with molecular weight of 15.2 kDa was extracted from Millettia speciosa Champ. leaf for the first time. Under the pH of 12.0, solid-liquid ratio of 1:40 (w/v), extraction time of 2.0 h, and extraction temperature of 50 °C, the highest extracting efficiency (79.25 ± 0.78%) of the Millettia speciosa Champ. leaf protein (MLP) was achieved. The main structure of MLP contained...

In silico identification and experimental validation of cellular uptake and intracellular labeling by a new cell penetrating peptide derived from CDN1

Xiangli Guo, Linlin Chen, Lidan Wang, Jingping Geng, Tao Wang, Jixiong Hu, Jason Li, Changbai Liu & Hu Wang
Bioactive therapeutic molecules are generally impermeable to the cell membrane, hindering their utility and efficacy. A group of peptides called cell-penetrating peptides (CPPs) were found to have the capability of transporting different types of cargo molecules across the cell membrane. Here, we identified a short peptide named P2, which has a higher proportion of basic residues than the CDN1 (cyclin-dependent kinase inhibitor 1) protein it is derived from, and we used bioinformatic analysis and experimental...

In silico identification and experimental validation of cellular uptake and intracellular labeling by a new cell penetrating peptide derived from CDN1

Xiangli Guo, Linlin Chen, Lidan Wang, Jingping Geng, Tao Wang, Jixiong Hu, Jason Li, Changbai Liu & Hu Wang
Bioactive therapeutic molecules are generally impermeable to the cell membrane, hindering their utility and efficacy. A group of peptides called cell-penetrating peptides (CPPs) were found to have the capability of transporting different types of cargo molecules across the cell membrane. Here, we identified a short peptide named P2, which has a higher proportion of basic residues than the CDN1 (cyclin-dependent kinase inhibitor 1) protein it is derived from, and we used bioinformatic analysis and experimental...

Mineral element contents and gene expression in Sophora tonkinensis during florescence

Lin-xuan Li, Zhu Qiao, Jin-yuan Cai, Xiao-yu Gu, Ying Liang, Namuhan Chen, Min-hui Li, Xiao-yun Guo, Jian-hua Miao & Kun-hua Wei
As a medicinal plant, Sophora tonkinensis is mainly distributed in Guangxi, Yunnan, Guizhou and northern Vietnam. In recent years, due to the strong demand for S. tonkinensis, wild resources have become decreased, which has placed great pressure on their protection. Therefore, the introduction of S. tonkinensis was carried out in Guangxi. Although the flowering period of S. tonkinensis is long, its seed setting rate is low. To promote flower production, it is necessary to do...

Biosynthesized CuO as a Green and Efficient Nanophotocatalyst in the Solvent-Free Synthesis of Some Chromeno[4, 3-b]Chromenes. Studying anti- Gastric Cancer Activity

Juan Liu, Jun Wang, Effat Esmaeili, Nasrin Mollania, Hengameh Atharifar, Maryam Keywanlu & Reza Tayebee
Gastric cancer is a foremost one among many regular carcinoma cases globally and most of the enhanced treatments could not significantly decrease the mortality range of this cancer. The current work is planned to develop a new rote for the synthesis of a nanophotocatalyst and substituted chromenones that are effective drugs against gastric cancer. Thus, CuO as a new heterogeneous green nanophotocatalyst is biosynthesized from Cressa leaf extract and the prepared nanophotocatalyst is applied in...

Enhanced oral bioavailability of koumine by complexation with hydroxypropyl-β-cyclodextrin: preparation, optimization, ex vivo and in vivo characterization

Qing Hu, Xiaoling Fu, Yanping Su, Yanfang Wang, Sihuan Gao, Xiaoqin Wang, Ying Xu & Changxi Yu
Koumine (KME) is an active alkaloid extracted from Gelsemium elegans, and its diverse bioactivities have been studied for decades. However, KME exhibits poor solubility and low oral bioavailability, which hampers its potential therapeutic exploitation. This work aimed to develop optimized inclusion complexes to improve the bioavailability of KME. The KME/hydroxypropyl-β-cyclodextrin (KME/HP-β-CD) inclusion complexes were prepared by the solvent evaporation method and later optimized using the Box-Behnken design. The optimal KME/HP-β-CD was characterized by scanning electron...

Identification and validation of potential mRNA- microRNA- long-noncoding RNA (mRNA-miRNA-lncRNA) prognostic signature for cervical cancer

Jie Wang & Chen Zhang
Cervical cancer is one of the most common causes of cancer deaths in women due to poor prognosis and high mortality rates. A novel mRNA-miRNA-lncRNA signature linked to prognosis of cervical cancer is needed to help clinicians judge the prognosis of individual patients more accurately. On the basis of GEO datasets, a total of 161 upregulated and 242 downregulated DE-mRNAs were identified firstly. Among them, eight potential biomarkers were found to have prognostic values with...

Cytotoxicity and polyol pathway inhibitory activities of chemical constituents isolated from the pericarp of Toona sinensis

Rongshen Wang, Di Liu, Xiaoxiao Liu, Fan Liu, Lulu Xuan, Ying Tang & Wanzhong Li
Toona sinensis is a medicinal and edible plant that belongs to the genus Toona of family Meliaceae. Phytochemical investigations carried out on this plant, seven apotirucallane-type triterpenoids (1–7), two cycloartane-type triterpenoids (8–9), four sterols (10–13), two sesquiterpenes (14–15), four phenols (16–19), and one lignin (20) were isolated from the pericarp of T. sinensis by silica gel column and preparative middle pressure liquid chromatography. Their structures were identified by interpretation of NMR and comparison with those...

Registration Year

  • 2021

Resource Types

  • Text


  • Chinese Academy of Medical Sciences & Peking Union Medical College
  • Shanghai Jiao Tong University
  • Zhejiang University
  • Sichuan University
  • Fudan University
  • Huazhong University of Science and Technology
  • Chinese Academy of Sciences
  • Capital Medical University
  • Sun Yat-sen University
  • University of Chinese Academy of Sciences