Additional file 4. Illustration of the seven-step cancer-immunity cycle. The seven-step cancer-immunity cycle encompasses the production of tumor antigens (step 1), presentation of tumor antigen (step 2), priming, and activation (step 3), T cells transfer to tumors (step 4), immune cell infiltration into tumors (step 5), tumor cell detection by T cells (step 6), and tumor cells apoptosis (step 7).

Additional file 7: Figure S4. Comparison of the three immunotypes according to the degree of CD8 infiltration. A: Comparison of the three immunotypes at the front of the tumor (CD68, Foxp3, PD-L1 and Ki67). C: Comparison of the three immunotypes at the center of the tumor (CD68, Foxp3, PD-L1 and Ki67). (*p< 0.05, p ≥ 0.05, not significant).

In this study, the biotransformation of asiatic acid by Cunninghamella echinulata CGMCC 3.970 and Circinella muscae CGMCC 3.2695 was investigated. Scaled-up biotransformation reactions yielded eight metabolites. Their structures were established based on extensive NMR and HR-ESI-MS data analyses and four of them are new compounds. C. echinulata could catalyze the regioselecitve hydroxylation, carbonylation and lactonization to yield five metabolites. C. muscae could selectively catalyze hydroxylation, acetylation and glycosylation to yield four products. Furthermore, all the...

Potentilla kleiniana Wight et Arn（PK, ‘Wu Pi Feng’ in Chinese） was recorded as Miao ethnic medicine for treatment of fever, cough, ulcer, and erysipelas for thousands years. This study aimed to evaluate the antiviral activity of four PK extracts and seven compounds by using HIV-1 protease (HIV-1 PR). In addition, Ultra-High Performance Liquid Chromatography and High Resolution Mass Spectrometry (UPLC-HRMS) was employed to identify the bioactive components. The toxicity assessment of the extracts was done...

Additional file 1.

Additional file 1.

An electro-swirling coupling device coupled with electric field and centrifugation can achieve demulsification and dewatering of emulsion efficiently and economically. But, the vortex structure and its implication on separation performance under the coupling condition are unclear. Accordingly, a vortex analysis for the coupling separator was conducted numerically, and the effect of eccentricity on the efficiency was investigated. The results show that the iso-vortex surface is twisted and fluctuates. And there is a vibrational radial deviation...

Additional file 1: Table S1. COVID-19 daily reported cases and response policies by local government. Table S2. Variable definitions and parameter values. Table S3. PHMS-Index calculation. Table S4. Intervention simulation corresponding parameters. Table S5. Parameter estimation of actual spillover rate and spillover threshold. Table S6. Rt and PHMS-Index values at different stages. Table S7. Mean, standard deviation, Person correlation coefficient of Rt and PHSM_Index. Table S8. Simulation of different mask wear rates in 4 cities....

A marine antifouling compound, N-octyl-2-hydroxybenzamide (OHBA), inspired by ceramide and paeonol molecules, was created. First, methyl salicylate was synthesized with salicylic acid and methanol, followed by n-octylamine through an ester-amine condensation reaction. Fourier transform infrared spectroscopy, nuclear magnetic resonance spectroscopy, and mass spectrometry confirmed the characteristic structure of the OHBA compound. Bioassays showed that OHBA inhibits the growth of typical marine fouling organisms, such as Vibrio azureus, Navicula subminuscula, Ulva pertusa, Mytilus edulis, and Amphibalanus...

Additional file 3: Fig. S3. The impacts of somatic copy number alterations in PDAC cohort, related to Fig. 2. A Bar plots showing pathway enrichment of cis/trans-effect between CNA and protein (left) or phosphoprotein (right). B Distribution of IRF6 status between the younger patients’ group (≤ 60) and the older patients’ group (> 60) (left). The boxplot reveals the comparison of the mRNA (middle) and protein (right) abundance of IRF6 between the younger patients (≤...

Additional file 8: Fig. S8. The effects of diabetes on the proteogenomic characteristics of PDAC, related to Fig. 4. A The boxplot representing the comparison of blood glucose concentration between patients with diabetes and without diabetes. B The Kaplan-Meier curves for overall survival based on diabetic patients with or without medical treatment. C The heatmap showing the distribution of 10q25.3 deletion and MTOR mutations across different samples in TCGA cohort. D The bar plot showing...

In this study, a superconducting high gradient magnetic separation (S-HGMS) technology was used to separate and extract quartz from sea sand. Under optimal conditions (i.e., a magnetic flow ratio of 0.085 T·s/m, slurry flow velocity of 400 ml/min, and pulp concentration of 40 g/L), the SiO2 grade increased from 79.45% of the raw material to 91.35% of quartz concentrate, and the removal rate of Al and Fe reached 91.97% and 82.10%, respectively. Weakly magnetic particles...

Additional file 5: Figure S3. The wild rice sites. The three in situ natural reserve sites (A) AJSI, (B) STSI and (C) ZTI and three ex situ artificial protection nurseries (D) AJS, (E) STS and (F) ZT. (In Figure C, professor Dazhou Chen who is one of the initiators for Dongxiang wild rice conservation is observing the growth status of wild rice).

Additional file 2: Table S2. Details of the sampling sites.

The association between T-cell large granular lymphocytes (T-LGL) and ITP is uncertain. The aims of this study were to determine the prevalence of T-LGL in patients with ITP and to describe its association with ITP disease severity. We analyzed flow cytometry results for T-LGL (using a threshold of 0.3 x109 or greater cells/L) or positive T-cell receptor clonality in patients with ITP and nonimmune thrombocytopenia. Descriptive statistics were used to characterize the association between T-LGL...

Supplementary Material 2

Additional file 12: Table S5. Primer Sets for qRT‒PCR and Mouse Genotyping.

Additional file 13: Table S6. Antibodies Used for Immunoblots.

Additional file 1. Supplementary Figure.

Additional file 1. Supplementary Figure.

Additional file 2. Supplementary Table.

Supplemental Material for Does Long-Term Shift Work Increase the Risk of Dementia? A Systematic Review and Meta-Analysis by Yang Hai, Ying Xue, and Yu-hong Wang in American Journal of Alzheimer's Disease & Other Dementias®

Supplemental material, sj-docx-1-hol-10.1177_09596836221145362 for Lake level evidence for a mid-Holocene East Asian summer monsoon maximum and the impact of an abrupt late-Holocene drought event on prehistoric cultures in north-central China by Zhiping Zhang, Zhongwei Shen, Shanjia Zhang, Jie Chen, Shengqian Chen, Dongxue Li, Shuai Zhang, Xiangjun Liu, Duo Wu, Yongwei Sheng, Qiuhong Tang, Fahu Chen and Jianbao Liu in The Holocene

In continuation of our previous work of small boron clusters [Phys. Chem. Chem. Phys. 23, 24118 (2021)], we systematically examined the neutral and negatively charged boron clusters Bn/Bn′ − (nε{8–38}) with planar or quasi-planar conformations, whose exotic properties have aroused considerable interest in the literature. We make use of two total energy partition schemes, conceptual density functional theory, and the information-theoretic approach to appreciate the cooperativity and reactivity properties. We found that neutral boron clusters...

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