Additional file 2: Supplementary tables.

Detailed discussion and supplemental data

Additional file 1: Table S1. List of all the participants. Table S2. The association between health behaviors and mental health among college students. Table S3. Mental health scores stratified by depression, anxiety symptoms. Table S4. The mediation effect of coping style on the relationship between lifestyle health behaviors and mental health symptoms in college students. Table S5. Model characteristics for the conditional process analysis. Table S6. Bootstrapped conditional direct and indirect effects. Fig. S1. The...

supporting information

Detailed discussion and supplemental data

Detailed discussion and supplemental data

Experimental characterization

supporting information

Additional file 3: Supplementary Table S3. Clinicopathological features of ESCC patients for plasma miR-637 analysis.

Additional file 6: Supplementary Fig. S3. Characteristics of KYSE70 cells with stable WASH knockdown. A, B Knockdown of WASH expression in KYSE70 cells through stable transduction with WASH shRNA (shWASH) or negative control shRNA (shNC) was validated by qRT-PCR (A) and Western blotting (B) assays, respectively. C The expression level of IL-8 was determined by qRT-PCR assay in KYSE70 cells with shRNA-mediated stable knockdown of WASH. Data are presented as mean ± standard deviation. ***p...

Additional file 8: Supplementary Fig. S5. Plasma IL-8 has no relationship with TNM stage and lymph node metastasis in ESCC patients. The expression levels of IL-8 in the plasma of ESCC patients were detected by ELISA assay and analyzed with TNM stage (A) and lymph node metastasis (B). ns, not significant.

Additional file 1: Supplementary Table S1. Primer Sequences for qRT-PCR assays.

Additional file 4: Supplementary Fig. S1. Expression of stemness-related genes detected by qRT-PCR assays. A, B Regulation of SOX4, SOX9, Nanog, CD44 and ABCG2 expression in KYSE70 cells and KYSE450 cells after treatment with miR-637 mimic (A) or miR-637 inhibitor (B), respectively. C, D KYSE450 cells were stably transduced with lentivirus overexpressing (OE) negative control (NC) or IL-8. Identification of IL-8 overexpression (C) and stemness-related gene expression after treatment with miR-637 mimic (D). Data are...

Additional file 5: Supplementary Fig. S2. IL-8 is involved in miR-637-regulated cancer stemness of ESCC cell lines. KYSE70 cells (A, B) and KYSE450 cells (C, D) were transfected with negative control (NC) or miR-637 mimic in the presence of control or recombinant human IL-8 (100 ng/ml). Tumor sphere formation (A, C) and qRT-PCR (B, D) assays were performed respectively. Scale bar, 200 μm. Data are presented as mean ± standard. *p < 0.05, **p <...

Detailed discussion and supplemental data

Experimental and characterization details

Additional file 1. RHC and BPA procedure.

Additional file 1. STROBE Statement—Checklist of items that should be included in reports of cross-sectional studies Title of the study: Investigating health service availability and readiness for antenatal testing and treatment for HIV and syphilis in Papua New Guinea.

supplementary material 1

supplementary material 1

A wave of Omicron infections rapidly emerged in China in 2022, but large-scale data concerning the safety profile of vaccines and Coronavirus disease 2019 (COVID-19) infection features in liver transplant (LT) recipients have not been collected. Therefore, the aim of this study was to assess the protectiveness and safety profile of the inactivated vaccines in LT patients against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron variant infections. A multi-centre retrospective study was conducted in...

Additional file 1: Supplementary figures.

Additional file 2: Supplementary tables.

Additional file 8: Supplementary Fig. S5. Plasma IL-8 has no relationship with TNM stage and lymph node metastasis in ESCC patients. The expression levels of IL-8 in the plasma of ESCC patients were detected by ELISA assay and analyzed with TNM stage (A) and lymph node metastasis (B). ns, not significant.

Additional file 2: Figure S1. The baseline echocardiographic and hemodynamic characteristics stratified by the modified COMERA 2.0 risk score. Figure S2. The baseline echocardiographic and hemodynamic characteristics stratified by the hybrid COMERA 2.0 risk score.