1,899 Works

OCTN2-targeted nanoparticles for oral delivery of paclitaxel: differential impact of the polyethylene glycol linker size on drug delivery in vitro, in situ, and in vivo

Longfa Kou, Rui Sun, Shuyi Xiao, Xiao Cui, Jin Sun, Vadivel Ganapathy, Qing Yao & Ruijie Chen
Targeted nanocarriers have shown great promise in drug delivery because of optimized drug behavior and improved therapeutic efficacy. How to improve the targeting efficiency of nanocarriers for the maximum possible drug delivery is a critical issue. Here we developed L-carnitine-conjugated nanoparticles targeting the carnitine transporter OCTN2 on enterocytes for improved oral absorption. As a variable, we introduced various lengths of the polyethylene glycol linker (0, 500, 1000, and 2000) between the nanoparticle surface and the...

Bicomponent polymeric micelles for pH-controlled delivery of doxorubicin

Chunyun Wang, Peilan Qi, Yan Lu, Lei Liu, Yanan Zhang, Qianli Sheng, Tianshun Wang, Mengying Zhang, Rui Wang & Shiyong Song
Stimuli-responsive drug delivery systems (DDSs) are expected to realize site-specific drug release and kill cancer cells selectively. In this study, a pH-responsive micelle was designed utilizing the pH-sensitivity of borate bonds formed between dopamine and boronic acid. First, methyl (polyethylene glycol)-block-polycaprolactone (mPEG-PCL) was conjugated with 4-cyano-4-(thiobenzoylthio)pentanoic acid (CTP) to obtain a macroinitiator. Two different segments poly(dopamine methacrylamide) (PDMA) and poly(vinylphenylboronic acid) (PVBA) were then grafted to the end of mPEG-PCL. Two triblock copolymers, mPEG-PCL-PDMA and...

Layer-by-layer pH-sensitive nanoparticles for drug delivery and controlled release with improved therapeutic efficacy in vivo

Wanfu Men, Peiyao Zhu, Siyuan Dong, Wenke Liu, Kun Zhou, Yu Bai, Xiangli Liu, Shulei Gong & Shuguang Zhang
In this work, a pH-sensitive liposome–polymer nanoparticle (NP) composed of lipid, hyaluronic acid (HA) and poly(β-amino ester) (PBAE) was prepared using layer-by-layer (LbL) method for doxorubicin (DOX) targeted delivery and controlled release to enhance the cancer treatment efficacy. The NP with pH-sensitivity and targeting effect was successfully prepared by validation of charge reversal and increase of hydrodynamic diameter after each deposition of functional layer. We further showed the DOX-loaded NP had higher drug loading capacity,...

Landscape of RNA editing reveals new insights into the dynamic gene regulation of spermatogenesis

Xiaodan Wang, Xiaolong Wu, Zhenshuo Zhu, Hao Li, Tongtong Li, Qun Li, Peng Zhang, Leijie Li, Dongxue Che, Xia Xiao, Tong Liu, Jinlian Hua & Mingzhi Liao
Spermatogenesis is an important physiological process associated with male infertility. As a kind of post-transcriptional regulation, RNA editings (REs) change the genetic information at the mRNA level. But whether there are REs and what’s the role of REs during the process are still unclear. In this study, we integrated published RNA-Seq datasets and established a landscape of RNA REs during the development of mouse spermatogenesis. Totally, 7530 editing sites occurred in 2012 genes among all...

Analytical comparisons of SARS-COV-2 detection by qRT-PCR and ddPCR with multiple primer/probe sets

Xinjin Liu, Jiangpeng Feng, Qiuhan Zhang, Dong Guo, Lu Zhang, Tao Suo, Wenjia Hu, Ming Guo, Xin Wang, Zhixiang Huang, Yong Xiong, Guozhong Chen, Yu Chen & Ke Lan
Different primers/probes sets have been developed all over the world for the nucleic acid detection of SARS-CoV-2 by quantitative real time polymerase chain reaction (qRT-PCR) as a standard method. In our recent study, we explored the feasibility of droplet digital PCR (ddPCR) for clinical SARS-CoV-2 nucleic acid detection compared with qRT-PCR using the same primer/probe sets issued by Chinese Center for Disease Control and Prevention (CDC) targeting viral ORF1ab or N gene, which showed that...

Co-delivery of doxorubicin and paclitaxel by reduction/pH dual responsive nanocarriers for osteosarcoma therapy

Yongshuang Li, Hao Hou, Peng Zhang & Zhiyu Zhang
Nanoparticle-based drug delivery system offers a promising platform for combination cancer therapy. However, the inefficient drug release in cells reduces the therapeutic efficacy of cancer nanomedicines. Herein, a PEGylated poly(α-lipoic acid) copolymer (mPEG-PαLA) was prepared and used as a reduction/pH dual responsive nanocarrier to simultaneously deliver paclitaxel (PTX) and doxorubicin (DOX) for osteosarcoma therapy. The amphiphilic mPEG-PαLA could efficiently encapsulate both PTX and DOX during its self-assembly into micelles in aqueous solution to generate PTX...

Profiling serum cytokines in COVID-19 patients reveals IL-6 and IL-10 are disease severity predictors

Huan Han, Qingfeng Ma, Cong Li, Rui Liu, Li Zhao, Wei Wang, Pingan Zhang, Xinghui Liu, Guosheng Gao, Fang Liu, Yingan Jiang, Xiaoming Cheng, Chengliang Zhu & Yuchen Xia
Since the outbreak of coronavirus disease 2019 (COVID-19) in Wuhan, China, it has rapidly spread across many other countries. While the majority of patients were considered mild, critically ill patients involving respiratory failure and multiple organ dysfunction syndrome are not uncommon, which could result death. We hypothesized that cytokine storm is associated with severe outcome. We enrolled 102 COVID-19 patients who were admitted to Renmin Hospital (Wuhan, China). All patients were classified into moderate, severe...

Developing a scale to measure the social distance between tourism community residents

Min Chen, Jinhe Zhang, Jinkun Sun, Chang Wang & Jinhua Yang
Social distance is an important tool for evaluating the degree of intimacy between individuals that has been applied in various disciplines. However, while increasing attention has been paid to this topic, little effort has been invested in understanding the construct of social distance between tourism community residents. The purpose of this study was to develop a scale that would assist in understanding and evaluating the social distance between tourism community residents. First, focus group interviews,...

Immune responses of a CV-A16 live attenuated candidate strain and its protective effects in rhesus monkeys

Ting Yang, Tianhong Xie, Hua Li, Xia Song, Lei Yue, Xi Wang, Dong Shen, Kaili Ma, Qinfang Jiang, Runxiang Long, Rong Yang, Xin He, Ye Zhang, Zhongping Xie & Qihan Li
Coxsackievirus A16 (CV-A16) is a major causative pathogen of hand, foot, and mouth diseases (HFMDs). The licensed HFMD vaccine targets EV-A71 without cross-protection against CV-A16. Thus, a CV-A16 vaccine is needed. In this study, the immunogenicity and protective efficacy of a live attenuated CV-A16 candidate, K168-8Ac, were evaluated in a rhesus monkey model. Four passages of this strain (P35, P50, P60, and P70) were administered to monkeys, and its protective effect was identified. The immunized...

Human coronavirus dependency on host heat shock protein 90 reveals an antiviral target

Cun Li, Hin Chu, Xiaojuan Liu, Man Chun Chiu, Xiaoyu Zhao, Dong Wang, Yuxuan Wei, Yuxin Hou, Huiping Shuai, Jianpiao Cai, Jasper Fuk-Woo Chan, Jie Zhou & Kwok Yung Yuen
Rapid accumulation of viral proteins in host cells render viruses highly dependent on cellular chaperones including heat shock protein 90 (Hsp90). Three highly pathogenic human coronaviruses, including MERS-CoV, SARS-CoV and SARS-CoV-2, have emerged in the past 2 decades. However, there is no approved antiviral agent against these coronaviruses. We inspected the role of Hsp90 for coronavirus propagation. First, an Hsp90 inhibitor, 17-AAG, significantly suppressed MERS-CoV propagation in cell lines and physiological-relevant human intestinal organoids. Second,...

Two new sesquiterpenoid lactone derivatives from Lindera aggregata

Song-Song Wen, Yan Wang, Jia-Ping Xu, Qi Liu, Lei Zhang, Jing Zheng, Lin Li, Na Zhang, Xin Liu, Yu-Wen Xu & Zhen-Liang Sun
Two new sesquiterpenoid lactone derivatives, linderin A (1) and linderin B (2) comprising a sesquiterpenoid lactone and a methyl geranylhomogentisate moiety together with six known compounds were isolated from the roots of Lindera aggregata. Their chemical structures were elucidated using extensive spectroscopic analysis including 1 D, 2 D NMR, and HR-ESI-MS data and compared with previously reported data. The absolute configurations of 1 and 2 were assigned based on the electronic circular dichroism calculation. Compound...

Optimization of the extraction conditions and dermal toxicity of oil body fused with acidic fibroblast growth factor (OLAF)

Yongxin Guo, Yaying Li, Qian Wu, Xinxin Lan, Guodong Chu, Weidong Qiang, Muhammad Noman, Tingting Gao, Jinnan Guo, Long Han, Jing Yang, Xiaokun Li & Linna Du
Oil body (OB), a subcellular organelle that stores oil in plant seeds, is considered a new transdermal drug delivery system. With the increasing understanding of the OB and its main protein (oleosin), numerous studies have been conducted on OB as “carrier” for the expression of exogenous proteins. In our previous study, oil body fused with aFGF (OLAF) was obtained using a plant oil body expression system that had been preliminarily proven to be effective in...

Optimization of the extraction conditions and dermal toxicity of oil body fused with acidic fibroblast growth factor (OLAF)

Yongxin Guo, Yaying Li, Qian Wu, Xinxin Lan, Guodong Chu, Weidong Qiang, Muhammad Noman, Tingting Gao, Jinnan Guo, Long Han, Jing Yang, Xiaokun Li & Linna Du
Oil body (OB), a subcellular organelle that stores oil in plant seeds, is considered a new transdermal drug delivery system. With the increasing understanding of the OB and its main protein (oleosin), numerous studies have been conducted on OB as “carrier” for the expression of exogenous proteins. In our previous study, oil body fused with aFGF (OLAF) was obtained using a plant oil body expression system that had been preliminarily proven to be effective in...

Extraction and characterization of a functional protein from Millettia speciosa Champ. leaf

Si-Yuan Luo, Zhi Huang, Xi Chen, Min-Hua Zong & Wen-Yong Lou
Natural plant-derived protein with excellent bioactivities has attracted much attention so a functional protein with molecular weight of 15.2 kDa was extracted from Millettia speciosa Champ. leaf for the first time. Under the pH of 12.0, solid-liquid ratio of 1:40 (w/v), extraction time of 2.0 h, and extraction temperature of 50 °C, the highest extracting efficiency (79.25 ± 0.78%) of the Millettia speciosa Champ. leaf protein (MLP) was achieved. The main structure of MLP contained...

Computational study of novel inhibitory molecule, 1-(4-((2S,3S)-3-amino-2-hydroxy-4-phenylbutyl)piperazin-1-yl)-3-phenylurea, with high potential to competitively block ATP binding to the RNA dependent RNA polymerase of SARS-CoV-2 virus

Prem Prakash Sharma, Sumit Kumar, Sukrit Srivastava, Mitul Srivastava, Babban Jee, Nikolay Yu. Gorobets, Dhruv Kumar, Mukesh Kumar, Shailendra Asthana, Peng Zhang, Poonam, Martin Zoltner & Brijesh Rathi
For coronaviruses, RNA-dependent RNA polymerase (RdRp) is an essential enzyme that catalyses the replication from RNA template and therefore remains an attractive therapeutic target for anti-COVID drug discovery. In the present study, we performed a comprehensive in silico screening for 16,776 potential molecules from recently established drug libraries based on two important pharmacophores (3-amino-4-phenylbutan-2-ol and piperazine). Based on initial assessment, 4042 molecules were obtained suitable as drug candidates, which were following Lipinski’s rule. Molecular docking...

Acute kidney injury in idiopathic membranous nephropathy with nephrotic syndrome

Tianxin Chen, Ying Zhou, Xinxin Chen, Bo Chen & Jingye Pan
The impact of acute kidney injury (AKI) on the progression of renal function in idiopathic membranous nephropathy (iMN) with nephrotic syndrome (NS) patients have not yet been reported, we sought to investigate the incidence, clinical features and prognosis of AKI in iMN with NS patients and determine clinical predictors for progression from AKI to advanced chronic kidney disease (CKD) stage. We analyzed clinical and pathological data of iMN with NS patients retrospectively collected from Jan...

Alterations of oral microbiota in patients with panic disorder

Zunli Xie, Weiqing Jiang, Mingzhu Deng, Wei Wang, Xian Xie, Xia Feng, Yinping Shi, Xueyan Zhang, Dong Song, Ziyu Yuan & Yonggang Wang
The main characteristics of panic disorder (PD) include recurrent panic attacks and persistent worry, accompanied by other physical and cognitive symptoms. While recent studies have revealed that gut bacteria play an important role in anxiety and depression, little is known about the relationship between oral microbiota and PD. Therefore, the objective of this study was to explore a possible correlation between oral microbiota and PD. We conducted 16S rRNA sequencing to compare differences in the...

Protective effects of Dapagliflozin on the vulnerability of ventricular arrhythmia in rats with pulmonary artery hypertension induced by monocrotaline

Tianyou Qin, Bin Kong, Chang Dai, Zheng Xiao, Jin Fang, Wei Shuai & He Huang
Monocrotaline (MCT)-induced pulmonary artery hypertension (PAH) has been reported to cause right heart failure (RHF). Moreover, Right heart diseases have been determined to cause ventricular arrhythmia (VA). So we can conclude that MCT-induced PAH increases the incidence of VA. In addition, Previous studies have determined the benefits of Dapagliflozin (DA) on the cardiac system, but the responses of MCT-induced RHF to DA are not fully reported. So the present study sought to evaluate the effects...

Protective effects of Dapagliflozin on the vulnerability of ventricular arrhythmia in rats with pulmonary artery hypertension induced by monocrotaline

Tianyou Qin, Bin Kong, Chang Dai, Zheng Xiao, Jin Fang, Wei Shuai & He Huang
Monocrotaline (MCT)-induced pulmonary artery hypertension (PAH) has been reported to cause right heart failure (RHF). Moreover, Right heart diseases have been determined to cause ventricular arrhythmia (VA). So we can conclude that MCT-induced PAH increases the incidence of VA. In addition, Previous studies have determined the benefits of Dapagliflozin (DA) on the cardiac system, but the responses of MCT-induced RHF to DA are not fully reported. So the present study sought to evaluate the effects...

Assessing spatiotemporal variations and predicting changes in ecosystem service values in the Guangdong–Hong Kong–Macao Greater Bay Area

Jie Zhang, Xuecao Li, Chenchen Zhang, Le Yu, Jingzhe Wang, Xiangyin Wu, Zhongwen Hu, Zihan Zhai, Qingquan Li, Guofeng Wu & Tiezhu Shi
Rapid economic development and interference by human activities in rapid urbanization regions have caused great land use/land cover change (LUCC), which significantly affects ecosystem functions and services. It is crucial to assess the spatiotemporal evolution of ecosystem service value (ESV) in such regions, especially for the Guangdong–Hong Kong–Macao Greater Bay Area (GBA) in China. In this study, we investigated and predicted the effect of LUCC on the ESV in the GBA from 1990 to 2030...

Two types of immune infiltrating cells and six hub genes can predict the occurrence of myasthenia gravis in patients with thymoma

Ziyou Tao, Chao Lu, Shuai Gao, Peng Zhang, Yuan Chen, Yuanguo Wang, Zhaoyu Yang, Kai Xiong & Yuxin Liu
Thymoma is the most common primary mass in anterior mediastinum. Although associated with low malignancy, it is often accompanied by myasthenia gravis resulting in poor prognosis. Due to the dual factors of tumor immune tolerance and autoimmune reaction, it is urgent to understand the immune status of MG with thymoma. In this study, RNA sequencing data were obtained from the TCGA and GEO cohorts to identify differentially expressed messenger RNAs and infiltrated immune cells. A...

Two new cytisine-type alkaloids from the seeds of Thermopsis lanceolata

Peng Zhang, Ji-Bin Zou, Qiao An, Ping Yi, Chun-Mao Yuan, Lie-Jun Huang, Wei Gu, Zhan-Xing Hu & Xiao-Jiang Hao
Two new (1 and 2) cytisine-type alkaloids that were chemically inseparable isomers (present in a 1:1 ratio) were identified from the seeds of Thermopsis lanceolata R. Br. Their structures were elucidated by comprehensive spectroscopic data analysis (IR, UV, NMR, HRESIMS) and ECD calculation. Compound 1 displayed significant anti-tobacco mosaic virus (TMV) activity, while compounds 1 and 2 displayed moderate insecticidal activities against Aphis fabae with LC50 value of 43.15 and 46.47 mg/L, respectively.

Functional characterization of C-TERMINALLY ENCODED PEPTIDE (CEP) family in Brassica rapa L

Ziwen Qiu, Keqing Zhuang, Yiting Liu, Xiaomin Ge, Chen Chen, Songping Hu & Huibin Han
The small regulatory C-TERMINALLY ENCODED PEPTIDE (CEP) peptide family plays crucial roles in plant growth and stress response. However, little is known about this peptide family in Brassica species. Here, we performed a systematic analysis to identify the putative Brassica rapa L. CEP (BrCEP) gene family. In total, 27 BrCEP genes were identified and they were classified into four subgroups based on the CEP motifs similarity. BrCEP genes displayed distinct expression patterns in response to...

Functional characterization of C-TERMINALLY ENCODED PEPTIDE (CEP) family in Brassica rapa L

Ziwen Qiu, Keqing Zhuang, Yiting Liu, Xiaomin Ge, Chen Chen, Songping Hu & Huibin Han
The small regulatory C-TERMINALLY ENCODED PEPTIDE (CEP) peptide family plays crucial roles in plant growth and stress response. However, little is known about this peptide family in Brassica species. Here, we performed a systematic analysis to identify the putative Brassica rapa L. CEP (BrCEP) gene family. In total, 27 BrCEP genes were identified and they were classified into four subgroups based on the CEP motifs similarity. BrCEP genes displayed distinct expression patterns in response to...

Accumulation and translocation of selenium in a soil–rice system in Guangxi, China

Zhen Wang, Fei Pang & Wei Huang
Selenium (Se) is an essential element for the human body. Humans meet their basic Se requirements primarily through food consumption. The largest Se-rich contiguous area in China is located in the southeast of Guangxi. In this study, the Se accumulation and translocation in the Guangxi rice–soil system was assessed. Organically bound Se (O-Se) was found to be the main type of soil Se (72.5%), and had a good linear relationship with soil total Se (TSe)...

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