1,576 Works

Protective effects of Dapagliflozin on the vulnerability of ventricular arrhythmia in rats with pulmonary artery hypertension induced by monocrotaline

Tianyou Qin, Bin Kong, Chang Dai, Zheng Xiao, Jin Fang, Wei Shuai & He Huang
Monocrotaline (MCT)-induced pulmonary artery hypertension (PAH) has been reported to cause right heart failure (RHF). Moreover, Right heart diseases have been determined to cause ventricular arrhythmia (VA). So we can conclude that MCT-induced PAH increases the incidence of VA. In addition, Previous studies have determined the benefits of Dapagliflozin (DA) on the cardiac system, but the responses of MCT-induced RHF to DA are not fully reported. So the present study sought to evaluate the effects...

Protective effects of Dapagliflozin on the vulnerability of ventricular arrhythmia in rats with pulmonary artery hypertension induced by monocrotaline

Tianyou Qin, Bin Kong, Chang Dai, Zheng Xiao, Jin Fang, Wei Shuai & He Huang
Monocrotaline (MCT)-induced pulmonary artery hypertension (PAH) has been reported to cause right heart failure (RHF). Moreover, Right heart diseases have been determined to cause ventricular arrhythmia (VA). So we can conclude that MCT-induced PAH increases the incidence of VA. In addition, Previous studies have determined the benefits of Dapagliflozin (DA) on the cardiac system, but the responses of MCT-induced RHF to DA are not fully reported. So the present study sought to evaluate the effects...

Two new cytisine-type alkaloids from the seeds of Thermopsis lanceolata

Peng Zhang, Ji-Bin Zou, Qiao An, Ping Yi, Chun-Mao Yuan, Lie-Jun Huang, Wei Gu, Zhan-Xing Hu & Xiao-Jiang Hao
Two new (1 and 2) cytisine-type alkaloids that were chemically inseparable isomers (present in a 1:1 ratio) were identified from the seeds of Thermopsis lanceolata R. Br. Their structures were elucidated by comprehensive spectroscopic data analysis (IR, UV, NMR, HRESIMS) and ECD calculation. Compound 1 displayed significant anti-tobacco mosaic virus (TMV) activity, while compounds 1 and 2 displayed moderate insecticidal activities against Aphis fabae with LC50 value of 43.15 and 46.47 mg/L, respectively.

Effectiveness of lymphoplasmapheresis compared with therapeutic plasma exchange for thrombotic thrombocytopenic purpura: a retrospective evaluation

Zhimin Zhang, Xi Yuan, Yuanjun Jiang, Ning Li & Bijuan Li
Thrombotic thrombocytopenic purpura (TTP) is an acute life-threatening disease usually treated with therapeutic plasma exchange (TPE), but some patients are refractory to TPE. The study aimed to compare lymphoplasmapheresis (LPE), an innovative treatment for TTP based on plasma exchange, with TPE in TTP treatment. This retrospective study included patients with TTP treated at Xiang-Ya Hospital in China during 2009-2018. All patients with microangiopathic hemolysis and thrombocytopenia who received either LPE or TPE were included. The...

Discovery of novel paeonol-based derivatives against skin inflammation in vitro and in vivo

Jing Wu, Ren De Zhu, Guo Min Cao, Jun Cheng Du, Xin Liu, Liang Zhuo Diao, Zhao Yan Zhang, Yang Sheng Hu, Xin Hua Liu & Jing Bo Shi
T-LAK-cell-originated protein kinase (TOPK), a novel member of the mitogen-activated protein kinase family, is considered an effective therapeutic target for skin inflammation. In this study, a series (A − D) of paeonol derivatives was designed and synthesised using a fragment growing approach, and their anti-inflammatory activities against lipopolysaccharide (LPS)-induced nitric oxide production in RAW264.7 cells were tested. Among them, compound B12 yielded the best results (IC50 = 2.14 μM) with low toxicity (IC50 > 50...

Development of naringenin-O-alkylamine derivatives as multifunctional agents for the treatment of Alzheimer’s disease

Jing Yang, Yi Zhou, Yujuan Ban, Jing Mi, Ying He, Xinjuan Li, Zhengwei Liu, Keren Wang, Gaofeng Zhu, Wenmin Liu, Zhenghuai Tan & Zhipei Sang
In this study, a series of naringenin-O-alkylamine derivatives were designed and obtained by introducing an alkylamine fragment into the naringenin skeleton. The in vitro biological activity results revealed that compounds 5f and 7k showed good antioxidant activity with ORAC values of 2.3eq and 1.2eq, respectively. Compounds 5f and 7k were reversible and excellent huAChE inhibitors with IC50 values of 0.91 μM and 0.57 μM, respectively. Moreover, compounds 5f and 7k could inhibit self-induced Aβ1–42 aggregation...

Two new terpenoids with anti-inflammatory activity from the fruits of Arenga pinnata (Wurmb) Merr.

Jia-Tong Wu, Adnan Mohammed Algradi, Yan Liu, Jin-Hai Huo, Xiao-Mao Li, Bing-You Yang & Wei-Ming Wang
Two new terpenoids (1 and 2), arenterpenoid D (1) and pinnasesquiterpene A (2), along with 16 phenylpropanoids (3–18) and 8 known terpenoids (19–26) were isolated from 70% EtOH extract of the Arenga pinnata (Wurmb) Merr. fruits. Their structures were elucidated by spectroscopic methods including HR-ESI-MS, 1D, and 2D NMR. The absolute configuration of arenterpenoid D (1) was defined by X-ray crystallographic analysis. Furthermore, we evaluated the anti-inflammatory activity of all compounds, and outcomes showed that...

Two new terpenoids with anti-inflammatory activity from the fruits of Arenga pinnata (Wurmb) Merr.

Jia-Tong Wu, Adnan Mohammed Algradi, Yan Liu, Jin-Hai Huo, Xiao-Mao Li, Bing-You Yang & Wei-Ming Wang
Two new terpenoids (1 and 2), arenterpenoid D (1) and pinnasesquiterpene A (2), along with 16 phenylpropanoids (3–18) and 8 known terpenoids (19–26) were isolated from 70% EtOH extract of the Arenga pinnata (Wurmb) Merr. fruits. Their structures were elucidated by spectroscopic methods including HR-ESI-MS, 1D, and 2D NMR. The absolute configuration of arenterpenoid D (1) was defined by X-ray crystallographic analysis. Furthermore, we evaluated the anti-inflammatory activity of all compounds, and outcomes showed that...

Assessing spatiotemporal variations and predicting changes in ecosystem service values in the Guangdong–Hong Kong–Macao Greater Bay Area

Jie Zhang, Xuecao Li, Chenchen Zhang, Le Yu, Jingzhe Wang, Xiangyin Wu, Zhongwen Hu, Zihan Zhai, Qingquan Li, Guofeng Wu & Tiezhu Shi
Rapid economic development and interference by human activities in rapid urbanization regions have caused great land use/land cover change (LUCC), which significantly affects ecosystem functions and services. It is crucial to assess the spatiotemporal evolution of ecosystem service value (ESV) in such regions, especially for the Guangdong–Hong Kong–Macao Greater Bay Area (GBA) in China. In this study, we investigated and predicted the effect of LUCC on the ESV in the GBA from 1990 to 2030...

Stachydrine is effective and selective against blast phase chronic myeloid leukaemia through inhibition of multiple receptor tyrosine kinases

Ruixin Gu, Wei Zhang & Dandan Xu
Resistance to BCR-ABL tyrosine kinase inhibitor (TKI) is the cause of treatment failure in blast phase chronic myeloid leukaemia (BP-CML). Agents that act synergistically with BCR-ABL TKI are required to improve response. This work investigated the effects of stachydrine in CML. CML cells were treated with control or stachydrine at 20, 40 and 80 µM. Proliferation and apoptosis were examined after 72 h treatment. Combination studies were performed in four groups: control, TKI, stachydrine and...

Abietane diterpenoids with neuroprotective activities from Phlegmariurus carinatus

Yang Liu, Qiang Wang, Dong-Kun Zheng, De Zhang, Zhen Xie, Jia-Wei Hu, Xin-Hua Xie, Jing Li & Shu-Ping Jiang
Two new abietane diterpenoids, phlecarinatone A (1) and phlecarinatone B (2), along with two known analogues (3 and 4), were isolated from Phlegmariurus carinatus. The structures of 1 − 4 were unambiguously elucidated by comprehensive spectroscopic analyses. Abietane diterpenoids were isolated from the plant for the first time. All isolates were tested for their neuroprotective activities against H2O2-induced SH-SY5Y cells injury, and compound 2 showed moderate effect at the concentrations ranging from 5 ∼ 20...

Acetylation of SCFD1 regulates SNARE complex formation and autophagosome-lysosome fusion

Hong Huang, Qinqin Ouyang, Kunrong Mei, Ting Liu, Qiming Sun, Wei Liu & Rong Liu
SCFD1 (sec1 family domain containing 1) was recently shown to function in autophagosome-lysosome fusion, and multiple studies have demonstrated the regulatory impacts of acetylation (a post-translational modification) on macroautophagy/autophagy. Here, we demonstrate that both acetylation- and phosphorylation-dependent mechanisms control SCFD1ʹs function in autophagosome-lysosome fusion. After detecting a decrease in the extent of SCFD1 acetylation under autophagy-stimulated conditions, we found that KAT2B/PCAF catalyzes the acetylation of residues K126 and K515 of SCFD1; we also showed that...

Purification and properties of a novel trypsin inhibitor from ginkgo fruits and its antiproliferative effect in triple-negative breast cancer cells

Xiaohui Zhao, Dayu Zhou, Shiliang Ma, Kexin Zheng, Ying Li & Bo Huang
A novel low molecular mass ginkgo biloba trypsin inhibitor (GBTI) was isolated from ginkgo fruits (GF) by trypsin inhibitory activity-guided fractionation by using ammonium sulphate precipitation, followed by ultra-filtration, affinity chromatography and RP-HPLC. The molecular mass and amino acid sequence of GBTI was determined using ESI-MS and ESI-MS/MS, respectively. The structure of GBTI was identified as MKNLTVIPPICLKFPN, with a molecular mass of 1826 Da. GBTI was stable in the pH range of 4–8 and in...

Effects of Chloride, Sulfate and Magnesium Ions on the Biomineralization of Calcium Carbonate Induced by Lysinibacillus xylanilyticus DB1-12

Huaxiao Yan, Meiyu Huang, Tiantian Wang, Yudong Xu, Long Meng, Lanmei Zhao, Zuozhen Han, Jihan Wang, Maurice E. Tucker & Hui Zhao
Microbially-induced calcium carbonate precipitation (MICP) has been studied extensively. However, the effect of different sources of magnesium ions on calcium carbonate biomineralization has rarely been examined. In this study, Lysinibacillus xylanilyticus DB1-12 bacteria isolated from petroleum samples were used to biomineralize calcium ions under the action of different sources of magnesium (magnesium chloride and magnesium sulfate). The results show that calcium ion precipitation ratios were much higher in MgSO4 group experiments than in the MgCl2...

Research strategy for constructing a green infrastructure network based on spatial prioritization

Dongmeng Wang, Chang Liu, Yue Guo, Puxia Tang, Jie Jiao, Weihan Kong, Ling Zhang, Yiping Liu & Dezheng Kong
The destruction of the ecological system caused by urban expansion has led to the environmental deterioration, cities have become increasingly vulnerable. In this study, six districts and counties along the Yellow River in Zhengzhou were selected as the study area. First, green infrastructure elements were extracted by morphological spatial pattern analysis. Then, outside the urban areas, we used connectivity analysis to evaluate the importance of core areas, adopted minimum cumulative resistance model to extract potential...

Compound glycyrrhiza oral solution alleviates oxidative stress and inflammation by regulating SRC/MAPK pathway in chronic obstructive pulmonary disease

Xiaolin Xu, Mulan Huang, Xiaofeng Duan, Hongyu Liu, Wei Zhang & Dan Li
Patients with chronic obstructive pulmonary disease (COPD) suffer from persistent cough and breathlessness, which can be ameliorated by the Chinese herbal medicine glycyrrhiza. Furthermore, the SRC/MAPK pathway is activated in the process of oxidative stress and inflammation, which afflict COPD progression. Thus, this research aimed at dissecting the mechanism of compound glycyrrhiza oral solution (CGOS) relieving oxidative stress and inflammation in COPD via the SRC/MAPK pathway. After a COPD rat model was established using lipopolysaccharide...

A. caviae infection triggers IL-1β secretion through activating NLRP3 inflammasome mediated by NF-κB signaling pathway partly in a TLR2 dependent manner

Qiankun Yang, Jianguo Zhang, Feixue Liu, Huizhen Chen, Wei Zhang, Haitao Yang, Nana He, Jingquan Dong & Panpan Zhao
Aeromonas caviae, an important food-borne pathogen, induces serious invasive infections and inflammation. The pro-inflammatory IL-1β functions against pathogenic infections and is elevated in various Aeromonas infection cases. However, the molecular mechanism of A. caviae-mediated IL-1β secretion remains unknown. In this study, mouse macrophages (PMs) were used to establish A. caviae infection model and multiple strategies were utilized to explore the mechanism of IL-1β secretion. IL-1β was elevated in A. caviae infected murine serum, PMs lysates...

Discovery of benzochalcone derivative as a potential antigastric cancer agent targeting signal transducer and activator of transcription 3 (STAT3)

Jinyun Dong, Jing Yang, Wenkai Yu, Haobin Li, Maohua Cai, Jing-Li Xu, Han-Dong Xu, Yun-Fu Shi, Xiaoqing Guan, Xiang‑Dong Cheng & Jiang‑Jiang Qin
Gastric cancer remains a significant health burden worldwide. In continuation of our previous study and development of effective small molecules against gastric cancer, a series of benzochalcone analogues involving heterocyclic molecules were synthesised and biologically evaluated in vitro and in vivo. Among them, the quinolin-6-yl substituted derivative KL-6 inhibited the growth of gastric cancer cells (HGC27, MKN28, AZ521, AGS, and MKN1) with a submicromolar to micromolar range of IC50, being the most potent one in...

Four ionones and ionone glycosides from the whole plant of Rehmannia piasezkii

Jie Zhou, Guo-Ru Shi, Wan-Qi Zhang, Ruo-Yun Chen, Yan-Fei Liu & De-Quan Yu
Four new ionones and ionone glycosides (1–4) were isolated from the whole plant of Rehmannia piasezkii Maxim. Their planar structures as well as absolute configuration were confirmed via spectroscopic analysis, ECD calculation, and X-ray crystallography. Compounds 1–4 were tested for their cytotoxicity against five human tumor cell lines and ability to inhibit LPS-activated NO production in the BV2 cell line.

Molybdopterin biosynthesis pathway contributes to the regulation of SaeRS two-component system by ClpP in Staphylococcus aureus

Na Zhao, Yanan Wang, Junlan Liu, Ziyu Yang, Ying Jian, Hua Wang, Mahmoud Ahmed, Min Li, Taeok Bae & Qian Liu
In Staphylococcus aureus, the SaeRS two-component system is essential for the bacterium’s hemolytic activity and virulence. The Newman strain of S. aureus contains a variant of SaeS sensor kinase, SaeS L18P. Previously, we showed that, in the strain Newman, SaeS L18P is degraded by the membrane-bound protease FtsH. Intriguingly, the knockout mutation of clpP, encoding the cytoplasmic protease ClpP, greatly reduces the expression of SaeS L18P. Here, we report that, in the strain Newman, the...

Additional file 2 of Identification of HMGB2 associated with proliferation, invasion and prognosis in lung adenocarcinoma via weighted gene co-expression network analysis

Xie Qiu, Wei Liu, Yifan Zheng, Kai Zeng, Hao Wang, Haijun Sun & Jianhua Dai
Additional file 2: Figure S1. Original blot and images. Figure S2. HMGB2 expression is not correlated with survival of patients with LUSC.

METTL3-induced DLGAP1-AS2 promotes non-small cell lung cancer tumorigenesis through m6A/c-Myc-dependent aerobic glycolysis

Qiang Zhang, Yu Zhang, Hui Chen, Lei-Na Sun, Bin Zhang, Dong-Sheng Yue, Chang-Li Wang & Zhen-Fa Zhang
The critical roles of N6-methyladenosine (m6A) modification have been demonstrated by more and more evidence. However, the cross talk of m6A and long noncoding RNAs (lncRNAs) in non-small cell lung cancer (NSCLC) tumorigenesis is still unclear. Here, this work focused on the functions and molecular mechanism of m6A-modified lncRNA DLGAP1 antisense RNA 2 (DLGAP1-AS2) in NSCLC. Microarray analysis found that lncRNA DLGAP1-AS2 is upregulated in NSCLC cells. Clinical data showed that DLGAP1-AS2 high-expression was correlated...

Additional file 1 of Systemic immune-inflammation index predicts postoperative delirium in elderly patients after surgery: a retrospective cohort study

Yuxiang Song, Yungen Luo, Faqiang Zhang, Yulong Ma, Jingsheng Lou, Hao Li, Yanhong Liu, Weidong Mi & Jiangbei Cao
Additional file 1: Figure A1. The receiver operating characteristics (ROC) curve of SII for POD. Table A1. Association between SII as continuous variable and POD in different models. Table A2. Association between SII as categories variables used quartiles and POD in different models. Table A3. Association between SII as categories variables with a cut-off value of 650 and POD in different models. Table A4. Univariate logistic regression analyses for POD in the Model PSM.

Additional file 1 of Construct and clinical verification of a nurse-led rapid response systems and activation criteria

Yuchen Wu, Jiaming Wang, Fan Luo, Dan Li, Xue Ran, Xuanlin Ren, Lixiu Zhang & Jingyun Wei
Additional file 1.

Additional file 4 of Inactivation of the MSTN gene expression changes the composition and function of the gut microbiome in sheep

Chenchen Du, Xianhui Zhou, Ke Zhang, Shuhong Huang, Xiaolong Wang, Shiwei Zhou & Yulin Chen
Additional file 4: Supplementary Table 1. Composition and nutrient levels of basal diet.

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  • Wuhan University
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  • Shanghai Jiao Tong University
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  • Chinese Academy of Medical Sciences & Peking Union Medical College
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  • Fudan University
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  • Zhejiang University
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  • Capital Medical University
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  • Huazhong University of Science and Technology
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  • Sun Yat-sen University
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  • Peking University
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  • Sichuan University
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