217 Works

Molecular characterization and functional properties of avenin-like b gene TuALPb7As in Triticum urartu

Dong Cao, Fahui Ye, Caixia Zhao, Jicheng Shen, Ruijuan Liu, Bo Zhang, Yuhu Shen, Huaigang Zhang, Baolong Liu & Wenjie Chen
Avenin-like storage proteins affect the rheological properties and processing quality of common wheat. Triticum urartu, the A-genome donor of polyploid wheat, shows abundant variation in its glutenin subunits. In this study, the variability in avenin-like b genes was evaluated in 74 T. urartu accessions. High levels of polymorphism were demonstrated in the analyzed sequence, resulting in the discovery of 18 novel TuALPb7A alleles (TuALPb7A-A–TuALPb7A-R). There were nucleotide sequence differences in 18 locations, which, due to...

Weighted gene co-expression network analysis (WGCNA) to explore genes responsive to Streptococcus oralis biofilm and immune infiltration analysis in human gingival fibroblasts cells

Xia Chen & Jianfeng Ma
The correlation between oral bacteria and dental implants failure has been reported. However, the effect and mechanism of bacteria during dental implants is unclear. In this study, we explored key genes and candidate gene clusters in human gingival fibroblasts (HGF) cells in response to Streptococcus oralis biofilm through weighted gene co-expression network analysis (WGCNA) and differential genes analysis using gene expression matrix, GSE134481, downloaded from the Gene Expression Omnibus (GEO) database. We obtained 325 genes...

The synergistic antitumor activity of 3-(2-nitrophenyl) propionic acid-paclitaxel nanoparticles (NPPA-PTX NPs) and anti-PD-L1 antibody inducing immunogenic cell death

Xiao-Chuan Duan, Li-Yuan Peng, Xin Yao, Mei-Qi Xu, Hui Li, Shuai-Qiang Zhang, Zhuo-Yue Li, Jing-Ru Wang, Zhen-Han Feng, Guang-Xue Wang, Ai Liao, Ying Chen & Xuan Zhang
Cancer immunotherapy is a strategy that is moving to the frontier of cancer treatment in the current decade. In this study, we show evidence that 3-(2-nitrophenyl) propionic acid-paclitaxel nanoparticles (NPPA-PTX NPs), act as immunogenic cell death (ICD) inducers, stimulating an antitumor response which results in synergistic antitumor activity by combining anti-PD-L1 antibody (aPD-L1) in vivo. To investigate the antitumor immunity induced by NPPA-PTX NPs, the expression of both ICD marker calreticulin (CRT) and high mobility...

Alkaloids constituents from the roots of Phragmites australis (Cav.) Trin. ex Steud. with their cytotoxic activities

Ying Chen, Li Li, Li-Rui Jiang, Jia-Yin Tan, Li-Na Guo, Xiao-Li Wang, Wei Dong, Wen-Bao Wang, Ji-Kai Sun & Bo Song
Two new alkaloids, phranisines A–B (1–2), along with two known compounds, N-p-Coumaroyl serotonin (3) and N-p-coumaroyl-tryptamine (4), were isolated from the roots of Phragmites australis. The structures of 1–4 were established on the basis of extensive spectroscopic. The absolute configuration of compounds 1–2 were identified through quantum-chemical electronic circular dichroism (ECD) calculation compared with their experimental CD. All the isolated compounds were tested for their cytotoxic activities against HeLa and MCF-7 human cancer cell lines,...

Identification and validation of potential mRNA- microRNA- long-noncoding RNA (mRNA-miRNA-lncRNA) prognostic signature for cervical cancer

Jie Wang & Chen Zhang
Cervical cancer is one of the most common causes of cancer deaths in women due to poor prognosis and high mortality rates. A novel mRNA-miRNA-lncRNA signature linked to prognosis of cervical cancer is needed to help clinicians judge the prognosis of individual patients more accurately. On the basis of GEO datasets, a total of 161 upregulated and 242 downregulated DE-mRNAs were identified firstly. Among them, eight potential biomarkers were found to have prognostic values with...

Cytotoxicity and polyol pathway inhibitory activities of chemical constituents isolated from the pericarp of Toona sinensis

Rongshen Wang, Di Liu, Xiaoxiao Liu, Fan Liu, Lulu Xuan, Ying Tang & Wanzhong Li
Toona sinensis is a medicinal and edible plant that belongs to the genus Toona of family Meliaceae. Phytochemical investigations carried out on this plant, seven apotirucallane-type triterpenoids (1–7), two cycloartane-type triterpenoids (8–9), four sterols (10–13), two sesquiterpenes (14–15), four phenols (16–19), and one lignin (20) were isolated from the pericarp of T. sinensis by silica gel column and preparative middle pressure liquid chromatography. Their structures were identified by interpretation of NMR and comparison with those...

Integrative genomic analysis of N6-methyladenosine-single nucleotide polymorphisms (m6A-SNPs) associated with breast cancer

Zixue Xuan, Yiwen Zhang, Jinying Jiang, Xiaowei Zheng, Xiaoping Hu, Xiuli Yang, Yanfei Shao, Guobing Zhang & Ping Huang
Due to the important role of N6-methyladenosine (m6A) in breast cancer, single nucleotide polymorphisms (SNPs) in genes with m6A modification may also be involved in breast cancer pathogenesis. In this study, we used a public genome-wide association study dataset to identify m6A-SNPs associated with breast cancer and to further explore their potential functions. We found 113 m6A-SNPs associated with breast cancer that reached the genome-wide suggestive threshold (5.0E-05), and 86 m6A-SNPs had eQTL signals. Only...

Dynamics in perioperative neutrophil-to-lymphocyte*platelet ratio as a predictor of early acute kidney injury following cardiovascular surgery

Yang Li, Zhouping Zou, Yunlu Zhang, Bowen Zhu, Yichun Ning, Bo Shen, Chunsheng Wang, Zhe Luo, Jiarui Xu & Xiaoqiang Ding
In this study, we applied a composite index of neutrophil-lymphocyte * platelet ratio (NLPR), and explore the significance of the dynamics of perioperative NLPR in predicting cardiac surgery-associated acute kidney injury (CSA-AKI). During July 1st and December 31th 2019, participants were prospectively derived from the ‘Zhongshan Cardiovascular Surgery Cohort’. NLPR was determined using neutrophil counts, lymphocyte and platelet count at the two-time points. Dose-response relationship analyses were applied to delineate the non-linear odds ratio (OR)...

Novel mutation in TSPAN12 associated with familial exudative vitreoretinopathy in a Chinese pedigree

Zhen Song, Mo Li, Chang Wang, Yu Wang, Lihua Zhang, Na Li, Ruifang Yang & Ping Sun
Familial exudative vitreoretinopathy (FEVR) is a rare retinal disorder characterised by incomplete retinal vascular development. Symptoms vary widely from none to blindness even within the same family. Multiple genes related to the Wnt pathway have been found to be associated with FEVR. Recent studies identified tetraspanin 12 (TSPAN12) as a cause of the autosomal dominant inheritance form of FEVR. Here, we describe a novel TSPAN12 mutation in a Chinese family with FEVR. Targeted next-generation sequencing...

The effects of ground cover management on fruit yield and quality: a meta-analysis

Linfa Fang, Xiaojun Shi, Yong Zhang, Yehua Yang, Xueliang Zhang, Xiaozhong Wang & Yuting Zhang
An important management strategy for the development of sustainable agriculture in orchards is the management of ground cover. However, there is a knowledge gap regarding the effects of ground cover on fruit yield and quality. We performed a meta-analysis of 580 observations collected from 116 peer-reviewed field studies, to assess the effects of ground cover on fruit yield and quality indicators. Overall, ground cover significantly increased fruit yield, soluble solid content, sugar content, and vitamin...

Genomics-guided isolation and identification of active secondary metabolites of Bacillus velezensis BA-26

Buqing Wang, Chao Liu, Xuemiao Yang, Yana Wang, Feiyan Zhang, Huicai Cheng, Liping Zhang & Hongwei Liu
Here we report the discovery and identification of antibacterial substances from the secondary metabolites of a new strain Bacillus velezensis BA-26. The whole genome of B. velezensis BA-26 was sequenced. Its genome data were annotated, and four potentially unknown gene clusters and nine known secondary metabolite synthetic gene clusters were analyzed and excavated by antiSMASH software. Based on the predicted secondary metabolites from the genome of strain BA-26, the material identification of isolated and purified...

Computational study of novel inhibitory molecule, 1-(4-((2S,3S)-3-amino-2-hydroxy-4-phenylbutyl)piperazin-1-yl)-3-phenylurea, with high potential to competitively block ATP binding to the RNA dependent RNA polymerase of SARS-CoV-2 virus

Prem Prakash Sharma, Sumit Kumar, Sukrit Srivastava, Mitul Srivastava, Babban Jee, Nikolay Yu. Gorobets, Dhruv Kumar, Mukesh Kumar, Shailendra Asthana, Peng Zhang, Poonam, Martin Zoltner & Brijesh Rathi
For coronaviruses, RNA-dependent RNA polymerase (RdRp) is an essential enzyme that catalyses the replication from RNA template and therefore remains an attractive therapeutic target for anti-COVID drug discovery. In the present study, we performed a comprehensive in silico screening for 16,776 potential molecules from recently established drug libraries based on two important pharmacophores (3-amino-4-phenylbutan-2-ol and piperazine). Based on initial assessment, 4042 molecules were obtained suitable as drug candidates, which were following Lipinski’s rule. Molecular docking...

Two new sesquiterpenoid lactone derivatives from Lindera aggregata

Song-Song Wen, Yan Wang, Jia-Ping Xu, Qi Liu, Lei Zhang, Jing Zheng, Lin Li, Na Zhang, Xin Liu, Yu-Wen Xu & Zhen-Liang Sun
Two new sesquiterpenoid lactone derivatives, linderin A (1) and linderin B (2) comprising a sesquiterpenoid lactone and a methyl geranylhomogentisate moiety together with six known compounds were isolated from the roots of Lindera aggregata. Their chemical structures were elucidated using extensive spectroscopic analysis including 1 D, 2 D NMR, and HR-ESI-MS data and compared with previously reported data. The absolute configurations of 1 and 2 were assigned based on the electronic circular dichroism calculation. Compound...

Integrative genomic analysis of N6-methyladenosine-single nucleotide polymorphisms (m6A-SNPs) associated with breast cancer

Zixue Xuan, Yiwen Zhang, Jinying Jiang, Xiaowei Zheng, Xiaoping Hu, Xiuli Yang, Yanfei Shao, Guobing Zhang & Ping Huang
Due to the important role of N6-methyladenosine (m6A) in breast cancer, single nucleotide polymorphisms (SNPs) in genes with m6A modification may also be involved in breast cancer pathogenesis. In this study, we used a public genome-wide association study dataset to identify m6A-SNPs associated with breast cancer and to further explore their potential functions. We found 113 m6A-SNPs associated with breast cancer that reached the genome-wide suggestive threshold (5.0E-05), and 86 m6A-SNPs had eQTL signals. Only...

Accumulation and translocation of selenium in a soil–rice system in Guangxi, China

Zhen Wang, Fei Pang & Wei Huang
Selenium (Se) is an essential element for the human body. Humans meet their basic Se requirements primarily through food consumption. The largest Se-rich contiguous area in China is located in the southeast of Guangxi. In this study, the Se accumulation and translocation in the Guangxi rice–soil system was assessed. Organically bound Se (O-Se) was found to be the main type of soil Se (72.5%), and had a good linear relationship with soil total Se (TSe)...

Discovery of novel inhibitors of SARS-CoV-2 main protease

Lei Zheng, Yanmei Chen, Jingxiao Bao, Liping He, Suzhen Dong, Yifei Qi & John Z. H. Zhang
Corona Virus Disease 2019 (COVID-19), referred to as ‘New Coronary Pneumonia’, is a type of acute infectious disease caused by the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection. Mpro is one of the main targets for treating COVID-19. The current research on Mpro mainly focuses on the repurposing of old drugs, and there are only a few novel ligands that inhibit Mpro. In this research, we used computational free energy calculation to screen a...

Network analysis of COVID-19-related PTSD symptoms in China: the similarities and differences between the general population and PTSD sub-population

Fan Yang, Mingqi Fu, Ning Huang, Farooq Ahmed, Muhammad Shahid, Bo Zhang, Jing Guo & Paul Lodder
Background and Objectives: Prevalent Post-traumatic Stress Disorder (PTSD) negatively affected individuals during the COVID-19 pandemic. Using network analyses, this study explored the construct of PTSD symptoms during the COVID-19 pandemic in China to identify similarities and differences in PTSD symptom network connectivity between the general Chinese population and individuals reporting PTSD. Methods: We conducted an online survey recruiting 2858 Chinese adults. PTSD symptoms were measured using the PCL-5 and PTSD was determined according to the...

Hepatitis B virus middle surface antigen loss promotes clinical variant persistence in mouse models

Junyu Lin, Jing Li, Peilin Xie, Yue Han, Demin Yu, Jia Chen & Xinxin Zhang
Hepatitis B virus (HBV) middle surface antigen (MHBs) mutation or deletion occurs in patients with chronic HBV infection. However, the functional role of MHBs in HBV infection is still an enigma. Here, we reported that 7.33% (11/150) isolates of CHB patients had MHBs start codon mutations compared with 0.00% (0/146) in acute hepatitis B (AHB) patients. Interestingly, MHBs loss accounted for 11.88% (126/1061) isolates from NCBI GenBank, compared with 0.09% (1/1061) and 0.00% (0/1061) for...

Optimization, reconstruction and heterologous expression of the gene cluster encoding toluene/o-xylene monooxygenase from Pseudomonas stutzeri in Escherichia coli and its successive hydroxylation of toluene and benzene

Bo Wang, Feng Gao, Jing Xu, Jianjie Gao, Zhenjun Li, Lijuan Wang, Fujian Zhang, Yu Wang, Yongsheng Tian, Rihe Peng & Quanhong Yao
Biological treatment is a cost-effective and environmentally friendly technique for treating toluene. However, this process is limited by the type and quantity of toluene degraders that can be applied to different environments and methods. It is a feasible solution to constructing new genetic engineering organisms for toluene degradation through biotechnology. To facilitate genetic manipulation and improve gene expression, the toluene/o-xylene monooxygenase locus from Pseudomonas stutzeri was reconstructed and artificially synthesized. All six genes were optimized...

Trypanosoma evansi evades host innate immunity by releasing extracellular vesicles to activate TLR2-AKT signaling pathway

Ran Wei, Xin Li, Xiaocen Wang, Nan Zhang, Yuru Wang, Xichen Zhang, Pengtao Gong & Jianhua Li
Surra, one of the most important animal diseases with economic consequences in Asia and South America, is caused by Trypanosoma evansi. However, the mechanism of immune evasion by T. evansi has not been extensively studied. In the present study, T. evansi extracellular vesicles (TeEVs) were characterized and the role of TeEVs in T. evansi infection were examined. The results showed that T. evansi and TeEVs could activate TLR2-AKT pathway to inhibit the secretions of IL-12p40,...

Orientin mediates protection against MRSA-induced pneumonia by inhibiting Sortase A

Li Wang, Shisong Jing, Han Qu, Kai Wang, Yajing Jin, Ying Ding, Lin Yang, Hangqian Yu, Yan Shi, Qianxue Li & Dacheng Wang
Drug-resistant pathogenic Staphylococcus aureus (S. aureus) has severely threatened human health and arouses widespread concern. Sortase A (SrtA) is an essential virulence factor of S. aureus, which is responsible for the covalent anchoring of a variety of virulence-related proteins to the cell wall. SrtA has always been regarded as an ideal pharmacological target against S. aureus infections. In this research, we have determined that orientin, a natural compound isolated from various medicinal plants, can effectively...

Early Cretaceous rapid exhumation processes in the southern Great Xing’an Range, NE China: implications for extensional geodynamics

Wen Zhang, Shuangfeng Zhao, Wen Chen, Jingbo Sun, Ze Shen, Qiuyi Du & Zhi Li
The Mesozoic-Cenozoic geological evolution of the southern Great Xing’an Range (GXR) has long been controversial since it is located in the superposed region of different tectonic regimes and involves complex interactions. Here, we present low-temperature thermochronological data from granitoids emplaced in multiple magmatic stages to delineate the tectono-thermal history and further understand the geodynamic process. Most zircon and apatite (U-Th)/He ages, within error, indicate Early Cretaceous: the zircon average (U-Th)/He ages range from 146.7 ±...

GlyinsRNA: a webserver for predicting glycosylation sites on small RNAs

Chunmei Cui, Xiaobin Wu & Yuan Zhou
Versatile RNA modifications play important roles in post-transcriptional regulations of gene expression, among which glycosylation modifications on small RNAs emerge as a novel clade whose characteristics need further interrogations. Here, we demonstrated that the sequence pattern around RNA glycosylation sites was not random and could be exploited for glycosylation site prediction. A machine learning predictor, GlyinsRNA, which integrated multiple RNA sequence representation encodings, was established. GlyinsRNA achieved AUROC (area under the receiver operating characteristic curve)...

Taberdines L and M, two new alkaloids from Tabernaemontana divaricata

Lingling Han, Kepu Huang, Chen Chen, Wentao Zhu, Yehan Ma, Xiaojiang Hao, Hongping He & Yu Zhang
Two new alkaloids, taberdines L (1) and M (2), together with six known alkaloids, were isolated from the twigs and leaves of Tabernaemontana divaricata. Taberdine L (1) represents the first optically active natural member of the allo iboga class of alkaloids with the ethyl side chain in a bridgehead position. Their structures including absolute configuration were elucidated by a combination of MS, NMR, and ECD calculation. The in vitro cytotoxic activities of 1 and 2...

A new dihydroflavone and a new polyacetylene glucoside from Bidens parviflora

Fu-Jie Cai, Chuan-Hou Li, Xiao-Hui Sun, Lu Wang, Jin-Li Tian, Wei Zhao, De-Gang Kong, Qing Liu & Hong-Lei Zhou
A new dihydroflavone, 2(S)-isookanin-4′-methoxy-8-O-β-D-glucopyranoside (1), and a new polyacetylene glucoside, (10S)-tridecane-2E-ene-4,6,8-triyne-1-ol-10-O-β-D-glucopyranoside (2), along with seven known compounds (3–9), were isolated from the herb of Bidens parviflora Willd. The structures of all the extracted compounds were elucidated by HR-ESI-MS, 1 D and 2 D NMR spectra, as well as circular dichroism (CD).

Registration Year

  • 2021

Resource Types

  • Text


  • Chinese Academy of Sciences
  • Chinese Academy of Medical Sciences & Peking Union Medical College
  • Shanghai Jiao Tong University
  • University of Chinese Academy of Sciences
  • Zhejiang University
  • Fudan University
  • Huazhong University of Science and Technology
  • Sun Yat-sen University
  • Capital Medical University
  • Sichuan University