2,663 Works

Two new cytisine-type alkaloids from the seeds of Thermopsis lanceolata

Peng Zhang, Ji-Bin Zou, Qiao An, Ping Yi, Chun-Mao Yuan, Lie-Jun Huang, Wei Gu, Zhan-Xing Hu & Xiao-Jiang Hao
Two new (1 and 2) cytisine-type alkaloids that were chemically inseparable isomers (present in a 1:1 ratio) were identified from the seeds of Thermopsis lanceolata R. Br. Their structures were elucidated by comprehensive spectroscopic data analysis (IR, UV, NMR, HRESIMS) and ECD calculation. Compound 1 displayed significant anti-tobacco mosaic virus (TMV) activity, while compounds 1 and 2 displayed moderate insecticidal activities against Aphis fabae with LC50 value of 43.15 and 46.47 mg/L, respectively.

Discovery of novel paeonol-based derivatives against skin inflammation in vitro and in vivo

Jing Wu, Ren De Zhu, Guo Min Cao, Jun Cheng Du, Xin Liu, Liang Zhuo Diao, Zhao Yan Zhang, Yang Sheng Hu, Xin Hua Liu & Jing Bo Shi
T-LAK-cell-originated protein kinase (TOPK), a novel member of the mitogen-activated protein kinase family, is considered an effective therapeutic target for skin inflammation. In this study, a series (A − D) of paeonol derivatives was designed and synthesised using a fragment growing approach, and their anti-inflammatory activities against lipopolysaccharide (LPS)-induced nitric oxide production in RAW264.7 cells were tested. Among them, compound B12 yielded the best results (IC50 = 2.14 μM) with low toxicity (IC50 > 50...

Controlled synthesis of cube-like ZnSnO3 decorated by nickel-based films and electrochemical applications on lithium-sulfur batteries

Mianjiao Jiang, Jun Wu, Hongmei Guo, Hongfang Zhang, Manli Zhu & Xiaobang Xu
Transition metal oxide was considered as an effective cathode additive to depress ‘shuttle effect’ in lithium-sulfur (Li-S) batteries via forming a strong polar chemical bond with polysulfides. Here, we firstly synthesized unique S@Ni/NiO/ZnSnO3 microcubes as composite cathodes for Li-S batteries, in which Ni/NiO films were deposited onto surface of as-prepared ZnSnO3 microcubes via a hydrothermal reaction and later calcinations process. The S@Ni/NiO/ZnSnO3 composite showed attractive electrochemical performances with a high initial discharge-specific capacity of 1070...

New cytotoxic ergosterols from a plant-associated fungus Colletotrichum magnisporum

Yanqi Hao, Lan Wei, Li Li, Yanlei Wang, Ning Li, Yingni Pan & Yi Sun
Three new ergosterols, colletosterols A-C (1–3), together with two known analogues 4 and 5, were isolated from the endophytic fungus Colletotrichum magnisporum associated with the leaves of Rauvolfia verticillata by a bioassay-guided fractionation method. The new structures were elucidated on the basis of extensive spectroscopic analyses and electronic circular dichroism (ECD) calculations. All the ergosterols were evaluated for their cytotoxic activities against A549 and HeLa cell lines. Compounds 1–3 exhibited notable cytotoxicity with the IC50...

New cytotoxic ergosterols from a plant-associated fungus Colletotrichum magnisporum

Yanqi Hao, Lan Wei, Li Li, Yanlei Wang, Ning Li, Yingni Pan & Yi Sun
Three new ergosterols, colletosterols A-C (1–3), together with two known analogues 4 and 5, were isolated from the endophytic fungus Colletotrichum magnisporum associated with the leaves of Rauvolfia verticillata by a bioassay-guided fractionation method. The new structures were elucidated on the basis of extensive spectroscopic analyses and electronic circular dichroism (ECD) calculations. All the ergosterols were evaluated for their cytotoxic activities against A549 and HeLa cell lines. Compounds 1–3 exhibited notable cytotoxicity with the IC50...

BUB1B (BUB1 Mitotic Checkpoint Serine/Threonine Kinase B) promotes lung adenocarcinoma by interacting with Zinc Finger Protein ZNF143 and regulating glycolysis

Xiaolei Zhou, Yanli Yuan, Hongping Kuang, Bingxiang Tang, Hui Zhang & Manlin Zhang
Lung adenocarcinoma (LUAD) is one of the most common causes of cancer death in men. BUB1B (BUB1 mitotic checkpoint serine/threonine kinase B) has been reported to contribute to the initiation and development of several cancers. Here, we aimed to explore the potential role of BUB1B in LUAD. We found BUB1B was upregulated in LUAD, suggesting its potential role as a biomarker for LUAD diagnosis. Significantly, LUAD patients with high BUB1B expression had a shorter survival...

The Effect of the Acidic Gas Pollutant on Wool Keratin Fiber Structure

Zhe Jiang, Rubing Bai, Jinfang Zhang, Ende Li, Yifan Cui, Ning Zhang, Nan Zhang & Qiang Wang
Increase in air pollution caused by acidic gases, such as nitrogen dioxide, call for the need to study potential effects on the structure of textile materials. Wool is a natural protein fiber which is easily damaged due to rich reaction positions and cross-linked bonds in the fiber. This study reports, for the first time, effect of nitrogen dioxide, as a model acid gas pollutant, on the structure of wool. On the macro level, wool fabric...

Ubiquitin-specific peptidase 2 inhibits epithelial-mesenchymal transition in clear cell renal cell carcinoma metastasis by downregulating the NF-κB pathway

Jiachen Duan, Mengyuan Jin, Dongjing Yang, Jihua Shi, Jie Gao, Danfeng Guo, Hongwei Tang, Shuijun Zhang & Baoping Qiao
Clear cell renal cell carcinoma, the most common type of renal cancer, is associated with poor survival. Ubiquitin-specific peptidase 2 regulates the molecular mechanisms of cancer cells. However, its mechanism in clear cell renal cell carcinoma remains unclear. Quantitative real-time polymerase chain reaction, enzyme-linked immunosorbent assay, and immunohistochemistry were performed to assess ubiquitin-specific peptidase 2 expression in human clear cell renal cell carcinoma samples. Ubiquitin-specific peptidase 2 was weakly expressed in clear cell renal cell...

Quantifying the relationship between biofilm reduction and thermal tissue damage on metal implants exposed to alternating magnetic fields

Bibin Prasad, Sumbul Shaikh, Reshu Saini, Qi Wang, Serena Zadoo, Varun Sadaphal, David E. Greenberg & Rajiv Chopra
Metal implant infections are a devastating problem due to the formation of biofilm which impairs the effectiveness of antibiotics and leads to surgical replacement as definitive treatment. Biofilm on metal implants can be reduced using heat generated by alternating magnetic fields (AMF). In this study, the relationship between implant surface biofilm reduction and surrounding tissue thermal damage during AMF exposure is investigated through numerical simulations. Mathematical models of biofilm reduction with heat were created based...

Nepetin reduces virulence factors expression by targeting ClpP against MRSA-induced pneumonia infection

Shisong Jing, Xinran Ren, Li Wang, Xiangri Kong, Xingye Wang, Xiren Chang, Xuerui Guo, Yan Shi, Jiyu Guan, Tiedong Wang, Bingmei Wang, Wu Song & Yicheng Zhao
The resistance of Staphylococcus aureus (S. aureus) to various antibiotics has increased dramatically due to the misuse of antibiotics, and thus the development of new anti-infective drugs with new targets is urgently needed to combat resistance. Caseinolytic peptidase P is a case in hydrolase that regulates the virulence level of S. aureus. Here, we found that nepetin, a small-molecule compound from traditional Chinese herbal flavonoids, effectively inhibits ClpP activity. Nepetin suppressed the virulence of S....

Stachydrine is effective and selective against blast phase chronic myeloid leukaemia through inhibition of multiple receptor tyrosine kinases

Ruixin Gu, Wei Zhang & Dandan Xu
Resistance to BCR-ABL tyrosine kinase inhibitor (TKI) is the cause of treatment failure in blast phase chronic myeloid leukaemia (BP-CML). Agents that act synergistically with BCR-ABL TKI are required to improve response. This work investigated the effects of stachydrine in CML. CML cells were treated with control or stachydrine at 20, 40 and 80 µM. Proliferation and apoptosis were examined after 72 h treatment. Combination studies were performed in four groups: control, TKI, stachydrine and...

Wood vinegar alleviates photosynthetic inhibition and oxidative damage caused by Pseudomonas syringae pv. tabaci (Pst) infection in tobacco leaves

Yuan Ye, Sun Hongwei, Wang Yue, Xu Zisong, Han Shixin, He Guoqiang, Yin Kuide & Huihui Zhang
We used chlorophyll fluorescence technology and biochemical methods to analyze the effects of wood vinegar (WV) on the photosynthetic mechanism and reactive oxygen species metabolism of tobacco (Nicotiana tabacum L.) leaves infected by Pseudomonas syringae pv. tabaci (Pst). The results showed that Pst infection reduced the chlorophyll content and the activities of PSII and PSI, which inhibited the normal photosynthesis of tobacco leaves. However, pretreatment with WV alleviated the degradation of chlorophyll. Treatment with WV...

The vehicle routing problem with release dates and flexible time windows

Xinrui Sun, Kunpeng Li & Wenli Li
In the context of the increasing demands for home delivery of fresh food, this article addresses the terminal distribution problem in an urban environment, which is called the vehicle routing problem with flexible time windows and order release dates (VRPFTWRD). This study develops a mathematical model and presents a set of valid inequalities based on the problem characteristics. According to the model and the inequalities, a branch-and-cut algorithm (B&C) is proposed for solving the problem....

Hydrogen sulfide protects retina from blue light-induced photodamage and degeneration via inhibiting ROS-mediated ER stress-CHOP apoptosis signal

Sen Zhu, Xuan Li, Bingrong Dang, Fen Wu, Kexin Gou, Chunming Wang & Changjun Lin
Background: Hydrogen sulfide (H2S) is a small reducing gas molecule with various biological functions such as anti-oxidative, anti-apoptotic and anti-inflammatory activities. In this study, we investigated the therapeutic effects of exogenous H2S in the experimental models of retinal photodamage in vivo and in vitro. Methods: Rats with open eyelids were pretreated with H2S (80~120 μmol/kg) for 10 days and then continuously exposed to blue light (435~445nm, 11.2W/m2) for 8 h to establish in vivo experimental...

Multi-source information fusion technology for risk assessment of water inrush from coal floor karst aquifer

Bo Li, Wenping Zhang, Jie Long, Juan Fan, Mengyu Chen, Tao Li & Pu Liu
In order to effectively solve the technical problem of the prediction of coal floor water inrush, according to the spatial distribution differences of geological conditions, combined with the typical coal mine, a partition calculation map of the development depth of the mineral pressure failure zone was made, and the mining process under different geological conditions has been numerically simulated and analyzed. And the empirical formula is modified by the numerical simulation calculation results, the calculation...

Multi-source information fusion technology for risk assessment of water inrush from coal floor karst aquifer

Bo Li, Wenping Zhang, Jie Long, Juan Fan, Mengyu Chen, Tao Li & Pu Liu
In order to effectively solve the technical problem of the prediction of coal floor water inrush, according to the spatial distribution differences of geological conditions, combined with the typical coal mine, a partition calculation map of the development depth of the mineral pressure failure zone was made, and the mining process under different geological conditions has been numerically simulated and analyzed. And the empirical formula is modified by the numerical simulation calculation results, the calculation...

CircRNA circ_0005273 contributes to the cisplatin resistance of cervical cancer cells by sponging miR-133b

Lixia Wu, Jing Gao, Subin Liu, Yuling Jia, Cairan Li & Lihong Duan
Circular RNAs (circRNAs) have been found to play important roles in drug resistance of human neoplasms. The aim of this study was to explore the effect of circ_0005273 on cisplatin (DDP) resistance of cervical cancer (CC) cells and identify its underlying mechanism. The quantitative real-time polymerase chain reaction (qRT-PCR) was performed to analyse circ_0005273 and miR-133b expressions, and cell counting kit-8 (CCK-8), Hoechst 33258 staining and caspase-3 activity analysis were performed to evaluate cell proliferation...

CircRNA circ_0005273 contributes to the cisplatin resistance of cervical cancer cells by sponging miR-133b

Lixia Wu, Jing Gao, Subin Liu, Yuling Jia, Cairan Li & Lihong Duan
Circular RNAs (circRNAs) have been found to play important roles in drug resistance of human neoplasms. The aim of this study was to explore the effect of circ_0005273 on cisplatin (DDP) resistance of cervical cancer (CC) cells and identify its underlying mechanism. The quantitative real-time polymerase chain reaction (qRT-PCR) was performed to analyse circ_0005273 and miR-133b expressions, and cell counting kit-8 (CCK-8), Hoechst 33258 staining and caspase-3 activity analysis were performed to evaluate cell proliferation...

Elevated D-Dimer levels correlate with the development of hepatorenal syndrome and a poor outcome in patients with cirrhosis

Baode Chen, Jing Liu, Yongtao Li, Xuelin He, Cheng Zhou, Yu Chen & Min Zheng
Whether hemostatic status was correlated with the diverse types of acute kidney injury in cirrhotic patients is unclear. The present study aimed to investigate the relationship between hemostatic markers and the diverse types of acute kidney injury (AKI) in liver cirrhosis. Cirrhotic patients with consecutive treatment at the First Affiliated Hospital of Medicine School, Zhejiang University, were pooled in a cohort. Their demographic and clinical data, biochemistry parameters and hemostatic markers were assessed to identify...

UHPLC-QTOF-MS/MS based characterization of anti-tumor constituents in Ceratocarpus arenarius L. and identification of EGFR-TK inhibitors by virtual screening

Le Pan, Li Li, Lu Xu, Jiaojiao Zhang, Jiashan Li, Ming Gao, JinMen Yu, Lu Jin & Dongyu Lei
The constituents of Ceratocarpus arenarius L., as a traditional anticancer medicine of Kazakh, were firstly profiled with UHPLC-QTOF-MS/MS. The potential compounds against EGFR-TK were virtually screened. As a result, forty-four compounds were analyzed, including 18 flavonoids, 8 steroids, 4 phenolic acids, 9 fatty acids, 1 coumarin and 4 other compounds. Among them, 9 flavonoids, N-trans-Feruloyltyramine (5), stigmasterol (11) and carthamone (38) were recognized as potential key anti-tumor constituents of C. arenarius through docking to active...

Clinical significance of mitogen-activated protein kinase kinase kinases in hepatitis B virus -related hepatocellular carcinoma and underlying mechanism exploration

Zhengqian Liu, Xin Zhou, Peng Zheng, Chenheng Bu, Xiao’ou Yan, Haizhou Yu & Yong Xu
The purpose of this research was to explore the diagnostic/prognostic significance and prospective molecular mechanisms of mitogen-activated protein kinase kinase kinases (MAP3Ks) in hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC). Diagnostic/prognostic significance of MAP3Ks was screened in the GSE1450 data set and validated in the Guangxi cohort. Various bioinformatics tools were used to explore the biological functions of prognosis-related genes. Subsequently, molecular biology assays were used to verify the biological functions and molecular mechanisms of...

Abietane diterpenoids with neuroprotective activities from Phlegmariurus carinatus

Yang Liu, Qiang Wang, Dong-Kun Zheng, De Zhang, Zhen Xie, Jia-Wei Hu, Xin-Hua Xie, Jing Li & Shu-Ping Jiang
Two new abietane diterpenoids, phlecarinatone A (1) and phlecarinatone B (2), along with two known analogues (3 and 4), were isolated from Phlegmariurus carinatus. The structures of 1 − 4 were unambiguously elucidated by comprehensive spectroscopic analyses. Abietane diterpenoids were isolated from the plant for the first time. All isolates were tested for their neuroprotective activities against H2O2-induced SH-SY5Y cells injury, and compound 2 showed moderate effect at the concentrations ranging from 5 ∼ 20...

Disulfiram/Copper induces antitumor activity against gastric cancer via the ROS/MAPK and NPL4 pathways

Yao Liu, Xin Guan, Meiling Wang, Naixue Wang, Yutong Chen, Baolei Li, Zhuxuan Xu, Fangwei Fu, Cheng Du & Zhendong Zheng
Disulfiram (DSF) is an anti-alcoholism medication with superior antitumor activity and clinical safety; its antitumor mechanisms in gastric cancer (GC) have not been fully explored. In the present work, low nontoxic concentrations of copper (Cu) ions substantially enhanced DSF’s antitumor activity, inhibiting the proliferation and growth of GC cell lines. DSF/Cu elevated the generation of reactive oxygen species (ROS), and apoptosis was induced in an ROS-dependent manner. This process might involve primary inhibition GC by...

Increased mitophagy protects cochlear hair cells from aminoglycoside-induced damage

Yuhua Zhang, Qiaojun Fang, Hongfeng Wang, Jieyu Qi, Shan Sun, Menghui Liao, Yunhao Wu, Yangnan Hu, Pei Jiang, Cheng Cheng, Xiaoyun Qian, Mingliang Tang, Wei Cao, Shang Xiang, Chen Zhang, Jianming Yang, Xia Gao, Zheng Ying & Renjie Chai
Aminoglycosides exhibit ototoxicity by damaging mitochondria, which in turn generate reactive oxygen species that induce hair cell death and subsequent hearing loss. It is well known that damaged mitochondria are degraded by mitophagy, an important mitochondrial quality control system that maintains mitochondrial homeostasis and ensures cell survival. However, it is unclear whether dysregulation of mitophagy contributes to aminoglycoside-induced hair cell injury. In the current study, we found that PINK1-PRKN-mediated mitophagy was impaired in neomycin-treated hair...

Help or punishment: acute stress moderates basal testosterone's association with prosocial behavior

Huagen Wang, Zhen Zhen, Ruida Zhu, Bohai Yu, Shaozheng Qin & Chao Liu
The gonadal hormone testosterone is well-recognized to facilitate various behaviors for obtaining social status. A good reputation (i.e. competitive, generous, and trustworthy) is of crucial importance for acquiring high social status. It is unclear which type of reputation is preferred by individuals under the influence of testosterone. Given that the recent dual-hormone hypothesis emphasizes the modulating effect of stress (cortisol) on the influence of testosterone, it would be intriguing to test the role of stress-induced...

Registration Year

  • 2022
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    2,663

Affiliations

  • Chinese Academy of Sciences
    2,663
  • Chinese Academy of Medical Sciences & Peking Union Medical College
    1,746
  • Shanghai Jiao Tong University
    1,553
  • University of Chinese Academy of Sciences
    1,510
  • Fudan University
    1,463
  • Zhejiang University
    1,462
  • Sichuan University
    1,461
  • Sun Yat-sen University
    1,364
  • Capital Medical University
    1,314
  • Huazhong University of Science and Technology
    1,262