Additional file 3. SF4. Immunohistochemical staining of p53 protein was in normal and tumor tissues. In the HPA database. Immunohistochemical staining of p53 protein was lighter in normal tissues than in tumor tissues.

Additional file 2: Supplementary Fig 2. Conformational changes in the NR5A1 mutant. The residual at position 22 changes from a hydrophobic to hydrophilic amino acid with the G22C substitution; both the WT and NR5A1-Mut proteins show the formation of a hydrogen bond with Thr29.

Additional file 5: Supplementary Table 2. Prediction of the impact of the c.64G>T (p.G22C) NR5A1 variant.

Additional file 2: Table S1. Independent genome-wide significant lead SNPs associated with pulmonary embolism (PE) in the discovery stage and replication stage. Table S2. The allele frequency of identified loci in 1000Genomes. Table S3. Replication of associations for the known loci in our cohort. Table S4. Association results for genes that were significant in FUMA gene-based analysis. Table S5. Polygenic risk score variants. Table S6. Polygenic risk score (PRS) quantile and odds ratio (OR).

Additional file 1: Figure S1. The Tamura 3-parameter model T92+G+I was used to produce theMaximum likelihood tree of 16S rRNA.

Supplementary materials

Additional file 3: Figure S3. The Jones-Taylor-Thornton model JTT was used to produce theMaximum likelihood tree of PhoD.

more details about the experiment

detailed description of some measurements

Supplemental material, sj-docx-1-tam-10.1177_17588359221136759 for Immune checkpoint inhibitors plus chemotherapy in patients with locally advanced or metastatic pulmonary sarcomatoid carcinoma: a multicentric real-world study by Fei Zhou, Haoyue Guo, Xiaolong Zhou, Huikang Xie, Tian Tian, Wencheng Zhao, Guanghui Gao, Anwen Xiong, Lei Wang, Wei Li, Xiaoxia Chen, Yan Zhang, Jue Fan, Fengying Wu, Yongchang Zhang and Caicun Zhou in Therapeutic Advances in Medical Oncology

Supplemental material, sj-docx-2-tam-10.1177_17588359221136759 for Immune checkpoint inhibitors plus chemotherapy in patients with locally advanced or metastatic pulmonary sarcomatoid carcinoma: a multicentric real-world study by Fei Zhou, Haoyue Guo, Xiaolong Zhou, Huikang Xie, Tian Tian, Wencheng Zhao, Guanghui Gao, Anwen Xiong, Lei Wang, Wei Li, Xiaoxia Chen, Yan Zhang, Jue Fan, Fengying Wu, Yongchang Zhang and Caicun Zhou in Therapeutic Advances in Medical Oncology

Photodynamic therapy (PDT) has been extensively investigated as a spatiotemporally noninvasive and controllable modality for cancer treatment. However, the intracellular antioxidant systems mainly consisting of thioredoxin (Trx) and glutathione (GSH) significantly counteract and prevent reactive oxygen species (ROS) accumulation, resulting in a serious loss of PDT efficiency. To address this challenge, we propose that PDT can be improved by precisely blocking antioxidant systems. After molecular engineering and synergistic cytotoxic optimization, a DSPE-PEG2K-modified dual-drug nanoassembly (PPa@GA/DSPE-PEG2K...

Additional file 1. Analysis and interpretation process of fetal ES data.

Additional file 2: Table S1. Clinical characteristics of the fetal cases. Table S2. Fetuses with diagnostic or VUS results obtained additional new phenotypes during prenatal and/or postnatal period. Table S3. Fetuses with positive diagnostic results detected by ES. Table S4. Diagnostic rates in different malformation subgroups. Table S5. Diagnostic rates in relation to NT measurement range. Table S6. Number of variants analyzed in step 1 and 2- case by case. Table S7. Number of variants...

Additional file 1: Table S1. Summary of daily mortality and TV indices in warm, moderate, and cold season in France during the study period. Figure S1. Sensitivity analyses to change lag days for daily mean temperature and relative humidity (from 21 to 28 days). Figure S2. Sensitivity analyses to change df for daily mean temperature and relative humidity (3–6 df). Figure S3. Sensitivity analyses to replace daily mean temperature to daily maximum temperature or daily...

Additional file 2: Table S1. siRNA oligonucleotides. Table S2. Gene-specific primers used in qRT-PCR. Table S3. piRNA specific primers used in qRT-PCR. Table S4. Antibodies used in this study.

The nuclear receptor (FXR) plays essential roles in maintaining bile acid and lipid homeostasis by regulating diverse target genes. And its agonists were promising agents for treating various liver diseases. Nevertheless, the potential side effect of chronic FXR activation by specific agonists is not fully understood. In this study, we investigated the mechanism of FXR agonist WAY-362450 induced liver enlargement during treating liver diseases. We demonstrated that chronic ingestion of WAY-362450 induced liver hypertrophy instead...

Additional file 2. Model consent form (Chinese).

Additional file 3. SPIRIT checklist.

Additional file 6: Dataset 4. Lists for the up-regulated COPD-associated DEGs in monocytes mentioned in Fig. S3I.

We extend three robust tests – Wald-type, the likelihood ratio-type and F-type in functional linear models with the scalar dependent variable and the functional covariate. Based on the percentage of variance explained criterion, we use the functional principal components analysis and re-express a functional linear model to a finite regression. We investigate the theoretical properties of these robust testing procedures and assess the finite sample properties through the numerical simulation. In our experiments, the power...

Additional file 1: Fig S1. Representative flow cytometry gating strategy for early-exhausted (PD-1int) and terminally-exhausted (PD-1hi) CD8+ T cells isolated from the tumor of a LLC mouse.

The sense of agency (SoA) refers to the feeling of being in control of one’s actions and the subsequent consequence of these actions. Emotional context seems to alter the strength of sense of agency. The present study explored the influence of acute psychosocial stress on the SoA by means of the Trier Social Stress Test (TSST). Self-assessment manikin (SAM) and objective physiological indicators (e.g. heart rate, electrodermal activity, and salivary cortisol levels) were utilized to...

The sense of agency (SoA) refers to the feeling of being in control of one’s actions and the subsequent consequence of these actions. Emotional context seems to alter the strength of sense of agency. The present study explored the influence of acute psychosocial stress on the SoA by means of the Trier Social Stress Test (TSST). Self-assessment manikin (SAM) and objective physiological indicators (e.g. heart rate, electrodermal activity, and salivary cortisol levels) were utilized to...

Additional file 1. Additional materials and methods. Figure S1. MMAE inhibits the growth of pancreatic cancer cells. Figure S2. Effect of Mc-vc-PABC linker on growth of pancreatic cancer cells. Figure S3. Expression of the MUC1 protein in Capan-2 and CFPAC-1 xenograft tumor tissues.