Different primers/probes sets have been developed all over the world for the nucleic acid detection of SARS-CoV-2 by quantitative real time polymerase chain reaction (qRT-PCR) as a standard method. In our recent study, we explored the feasibility of droplet digital PCR (ddPCR) for clinical SARS-CoV-2 nucleic acid detection compared with qRT-PCR using the same primer/probe sets issued by Chinese Center for Disease Control and Prevention (CDC) targeting viral ORF1ab or N gene, which showed that...

Quantitative real time PCR (RT-PCR) is widely used as the gold standard for clinical detection of SARS-CoV-2. However, due to the low viral load specimens and the limitations of RT-PCR, significant numbers of false negative reports are inevitable, which results in failure to timely diagnose, cut off transmission, and assess discharge criteria. To improve this situation, an optimized droplet digital PCR (ddPCR) was used for detection of SARS-CoV-2, which showed that the limit of detection...

Supplemental figures

Additional file 2. MEDLINE draft search strategy.

Additional file 1: Fig. S1. Fragment recruitment analysis. Fig. S2. The relative abundance of transporter proteins in metaproteome. Fig. S3. Profiles of transporter genes in the assembled MAGs within Epsilonbacteraeota and Gammaproteobacteria.

Additional file 4: Fig. S4. CSGs plotted annotated on bacterial phylogeny comprising sponge-derived (Ircinia and non-Ircinia) and Tara Oceans MAGs, following tree construction and plotting scheme as outlined for Fig. 3.

Additional file 2: Figure S1. Crosstalk effect consideration detects genes known to be associated with LUAD. Figure S2. CGC genes are in top of the RS rank list, but not top of the |log2FC| rank list. Figure S3. The proportion of risk genes, overlooked by other methods. Figure S4. Comparison of DEG proportions between pathways specifically identified by CTpathway and non-specific pathways. Figure S5. Significant pathways in data sets with a small number of DEGs,...

Supplemental material, sj-docx-1-tag-10.1177_17562848221100106 for Impact of NAFLD on clinical outcomes in hepatocellular carcinoma treated with sorafenib: an international cohort study by Jessica Howell, Amit Samani, Binish Mannan, Saur Hajiev, Leila Motedayen Aval, Rebecca Abdelmalak, Vincent C. Tam, Dominik Bettinger, Robert Thimme, Tamar H. Taddei, David E. Kaplan, Max Seidensticker and Rohini Sharma in Therapeutic Advances in Gastroenterology

Additional file 2. Additional Methods. Fig. S1. The Kaplan–Meier curves of LVEF (A-B) and LVMI (C-D) for risks of death and MACE in the discovery cohort. Fig. S2. Correlation network of metabolic signatures for MACE risk and clinical factors. Fig. S3. Representative total ion flow diagrams between different QC samples under positive ion mode (A) and negative ion mode (B). Fig. S4. The visualization image analysis of the quality improvement procedures for typical features (Kynurenine)...

Additional file 2: Supplementary Fig. 1. The classification plot of samples from three cell lines based on all the DEGs. Cells were treated with (3, 12, 24 h.p.i.) or without (normal group) ZIKV infection; Supplementary Fig. 2. The expression heatmap of 33 common DEGs among JEG-3, U-251 MG, and HK-2 cells; Supplementary Fig 3. The enriched GO BPs of DEGs from 3 cell lines, including (a) JEG-3, (b) U-251 MG, (c) HK-2 cells. Supplementary Table...

Additional file 1: Figure S1. Proportions of different types of alteration in resection (A) and biopsy (B) specimens. CN_amp: copy number amplification; InDel: small insertion and deletion; LGR: large genomic rearrangement.

Additional file 1: Figure S1. Proportions of different types of alteration in resection (A) and biopsy (B) specimens. CN_amp: copy number amplification; InDel: small insertion and deletion; LGR: large genomic rearrangement.

Additional file 2: Figure S2. Comparative analyses of the sequencing depth (A) and allele frequency (B) between the matched alterations and specific alterations.

Additional file 3: Figure S3. The difference (A) and correlation (B) between resection specimen-based TMB and CNB-based TMB. RS: resection specimen; CNB: core needle biopsy; TMB: tumor mutation burden.

Additional file 1. Tetrandrine ameliorates cognitive deficits and mitigates tau aggregation in cell and animal models of tauopathies.

Additional file 1. Supplemental Tables.

Supplemental material, sj-docx-1-tah-10.1177_20406207221134409 for Association between haploidentical hematopoietic stem cell transplantation combined with an umbilical cord blood unit and graft-versus-host disease in pediatric patients with acquired severe aplastic anemia by Di Yao, Yuanyuan Tian, Jie Li, Bohan Li, Jun Lu, Jing Ling, Defei Zheng, Yanhua Yao, Peifang Xiao, Lijun Meng and Shaoyan Hu in Therapeutic Advances in Hematology

Additional file 1: Figure S1. Illustration of 3’UTR-seq method and quality control. A. Overview of the QuantSeq data analysis pipeline. B. Genome browser screenshot of extended 3’UTRs for genes Pou1f1 (left) and Ghrhr (right). Gene name and gene model are shown on the bottom of each panel. Each track represents overlapping signal from 5-6 biological replicates. C. Summary of qPCR vs. 3’UTR-seq comparisons across samples in all samples. Bottom panel: bar plots showing the numbers...

Sponges are animals that inhabit many aquatic environments while filtering small particles and ejecting metabolic wastes. They are composed of cells in a bulk extracellular matrix, often with an embedded scaffolding of stiff, siliceous spicules. We hypothesize that the mechanical response of this heterogeneous tissue to hydrodynamic flow influences cell proliferation in a manner that generates the body of a sponge. Towards a more complete picture of the emergence of sponge morphology, we dissected a...

We identified relapsing fever (RF) Borrelia in 1.45% (145/10426) of the ticks and 1.40% (40/2850) of the wild mammals in a field investigation in China. Three RF Borrelia species, including human-pathogenic Borrelia miyamotoi, Borrelia persica and unclassified Babesia sp. were determined. Main species determined from ticks was B. miyamotoi (44.14%), followed by the unclassified Borrelia sp. (42.76%), and Borrelia theileri (13.10%). In wild mammals, main species found was B. persica (57.50%), followed by the unclassified...

Additional file 1: Supplementary Figure 1. Light activated sensory neurons release inflammatory mediators. DRG sensory neurons harvested from Vglut2-Cre/ChR2-eYFP mice were cultured for 48–72 h and then stimulated with blue light (470 nm) at 20 Hz, 10% duty cycle for 15 min. Supernatant was harvested at indicated time points, and levels of HMGB1 (A), substance P (B) and CGRP (C) were quantified. N = 4-5 per group. *P < 0.05, **P < 0.01, ***P <...

Additional file 3: Supplementary Figure 3. Acute optogenetic stimulation does not induce hyperalgesia or the release of inflammatory mediators in wild type mice or primary sensory neurons. Wild type (C57BL/6) mice were anesthetized and subjected to optogenetic stimulation using 470 nm LED (blue) or 595 nm LED (yellow light) for 15 min on the dorsum of the right hind paw. (A) Mechanical hypersensitivity was assessed 5 h later, using von Frey filaments. Blue or yellow...

Iron oxide nanoparticles (IONPs) are mostly intended to be administrated intravenously, understanding the interaction of IONPs with vascular endothelial cells is extremely crucial for developing safe application regimes of IONPs. In this work, interactions of three kinds of IONPs to endothelial cells were investigated both in human umbilical vein endothelial cells (HUVECs) and in healthy mice. Both meso-2,3-dimercaptosuccinic acid (DMSA) coated Fe3O4 NPs (DMSA-Fe3O4 NPs) and DMSA-Fe2O3 NPs induced cell growth inhibition, while polyglucose sorbitol...

Icaritin (ICT) and hydrous icaritin (HICT) are two similar flavonoids compounds isolated from Epimedium Genus. This is the first comparative study on their in vitro and in vivo antitumor effects. Nanorods (NRs) were prepared for ICT and HICT by anti-solvent precipitation method using D-alpha tocopherol acid polyethylene glycol succinate (TPGS) as a stabilizer. The prepared ICT-NRs and HICT-NRs had similar diameter (155.5 nm and 201.7 nm), high drug loading content (43.30 ± 0.22% and 41.08...

Exposure to diabetes mellitus during pregnancy increases the risk of offspring obesity and cardiometabolic disease. Limited information exists regarding growth patterns among preterm infants exposed to maternal diabetes. This study describes growth differences during early childhood among late preterm infants of mothers with and without diabetes during pregnancy. In a retrospective longitudinal analysis, weight trajectories from birth to age 2 years were compared between diabetes exposure groups (N = 1554) using mixed effects modeling and...