In Staphylococcus aureus, the SaeRS two-component system is essential for the bacterium’s hemolytic activity and virulence. The Newman strain of S. aureus contains a variant of SaeS sensor kinase, SaeS L18P. Previously, we showed that, in the strain Newman, SaeS L18P is degraded by the membrane-bound protease FtsH. Intriguingly, the knockout mutation of clpP, encoding the cytoplasmic protease ClpP, greatly reduces the expression of SaeS L18P. Here, we report that, in the strain Newman, the...

Two new phenolic allopyranosides, named viburluzosides A and B (1, 2), together with eight known phenolic glycosides (3 − 10) were discovered from the stems of Viburnum luzonicum Rolfe under the guidance of LC-MS analyses coupled with bioactivity evaluation. They were purified through various chromatography methods and identified by extensive spectroscopic analyses (1H and 13C NMR, HSQC, HMBC, and HRESIMS) and chemical methods. The in vitro evaluation on α-glucosidase and aldose reductase (AR) inhibitory activities...

Additional file 7: Table S1. Growth rate of strain expressing various related genes in different medium.

Additional file 2. Additional Methods. Fig. S1. The Kaplan–Meier curves of LVEF (A-B) and LVMI (C-D) for risks of death and MACE in the discovery cohort. Fig. S2. Correlation network of metabolic signatures for MACE risk and clinical factors. Fig. S3. Representative total ion flow diagrams between different QC samples under positive ion mode (A) and negative ion mode (B). Fig. S4. The visualization image analysis of the quality improvement procedures for typical features (Kynurenine)...

Supplemental figures

Additional file 2: Figure S2. Articles uploaded to the WeChat officialaccount.

Additional file 3: Figure S3. Screenshots of parents and doctorscommunicating with each other in the WeChat group.

Additional file 6: Table S3. Acceptance of WeChat among intervention group and control group.

Additional file 1. The IMP2 protein sequences.

Additional file 5. Fig. S4. p53 exerts an important negative role in HG-induced fibrosis in vitro.

Additional file 5. Fig. S4. p53 exerts an important negative role in HG-induced fibrosis in vitro.

Additional file 6. Fig. S5. Cardiac fibroblasts isolated form the heart in CTRL+AAV9-shAirn mice share the same the cellular mechanisms in vitro.

Growing studies show that lipoprotein (a) [Lp(a)] is related to calcified aortic valve diseases in general population, while the relationship between Lp(a) and aortic valve calcification (AVC) in patients with new-onset acute myocardial infarction (AMI) remains unclear. Therefore, this study was to evaluate the correlation between Lp(a) and AVC in patients with new-onset AMI. This cross-sectional study included 410 patients with new-onset AMI who were hospitalised in Zhongda Hospital affiliated to Southeast University from January...

Additional file 1. Supplemental Data Appendix.

Additional file 1. Appendix. Detailed description of covariates, supplementary analyses not shown in main text, and raw data on cause of death classification.

Additional file 2: Fig. S1. Distribution of SNP number in different chromosomes and count of MAF. A, SNP numbers. B, SNP frequency in different minor allele frequency. Fig. S2. Distributions of SNP linkage disequilibrium decay and heterozygosity. A, Linkage disequilibrium decay (r2) across different linkage disequilibrium windows in 294 wild soybean accessions. B, Histogram of heterozygosity for SNPs. Fig. S3. Boxplot representing the distribution of flowering time in five active accumulated temperature zones in Heilongjiang...

To help move society towards more sustainable states, policies have been developed in various countries to create a circular bioeconomy (CBE) in biobased sectors such as forestry and agriculture. In operationalizing CBE, initiatives must be created in which feedback loops between life-cycle stages are established to enable a “stock” of resources to be recirculated in the economy. By creating such feedback loops, CBE aims to decouple economic growth from natural resource depletion and degradation. However,...

Additional file 2: Supplementary Fig. 1. The classification plot of samples from three cell lines based on all the DEGs. Cells were treated with (3, 12, 24 h.p.i.) or without (normal group) ZIKV infection; Supplementary Fig. 2. The expression heatmap of 33 common DEGs among JEG-3, U-251 MG, and HK-2 cells; Supplementary Fig 3. The enriched GO BPs of DEGs from 3 cell lines, including (a) JEG-3, (b) U-251 MG, (c) HK-2 cells. Supplementary Table...

Additional file 1: Supplementary Table 1. The four types of social isolation question and thescoring criteria for the composite social isolation score. Supplementary Table2. The Chinese version of four types of social isolation question and thescoring criteria for the composite social isolation score. Supplementary Table3. Associationof hearing loss with depressive symptoms (GDS-15 score ≥8). Supplementary Table4. Association of hearingloss with depressive symptoms after excluding participants with hearing aids (n=20).

The antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) is a systematic of relatively rare autoimmune diseases with unknown cause. Kidney involvement is one of the most common clinical manifestations, and the degree of renal damage is closely associated with the development and prognosis of AAV. In this study, we utilized the Robust Rank Aggreg (RRA) method in R to integrate GSE104948, GSE104954, GSE108109, GSE108112, and GSE108113 profile datasets loaded from Gene Expression Omnibus (GEO) database and...

In this work, a highly effective separation approach mediated by 5-Lipoxygenase (5-LOX) was established for screening and isolation of anti-inflammatory ingredients from leaves of Lonicera japonica Thunb. (LLJT). Using 5-LOX immobilised on TiO2 nanotubes as a microreactor, the targeted screening was exploited by combining with HPLC-MS system. Four compounds confirmed as luteolin, luteoside, lonicerin, and isochlorogenic acid C and a fraction (M1) were screened out to be potent inhibitors of 5-LOX. Their anti-inflammatory activities were...

As per the National Medical Products Administration (NMPA) requirements, the quality and efficacy of generic drugs must be consistent with those of the innovator drug. We aimed to evaluate the bioequivalence and safety of generic metformin hydrochloride sustained-release (MH-SR) tablets (Boke®) developed by Beijing Wanhui Double-crane Pharmaceutical Co. Ltd., China and the innovator product metformin hydrochloride extended-release tablets (Glucophage®-XR) manufactured by Bristol-Myers Squibb Company, New York, NY, in healthy Chinese volunteers. We performed a bioequivalence...

Supplementary Materials

Hydroxy-α-sanshool (HAS), extracted from Zanthoxylum piperitum, is commonly used in oral surgery to relief pain. However, the application of HAS is limited in clinical practice due to its poor stability. This study focuses on the design of a novel nano-formulation delivery system for HAS to improve its stability and local anesthetic effect. Hydroxy-α-sanshool loaded nanostructured lipid carriers (HAS-NLCs) were prepared by melting emulsification and ultra-sonication using monostearate (GMS) and oleic acid (OA) as lipid carriers,...